The aim of this paper is to contribute to the identification and characterization of the various types of intuition put forward by Poincar6, taking his texts as a laboratory for looking for what intuition might be. I ...The aim of this paper is to contribute to the identification and characterization of the various types of intuition put forward by Poincar6, taking his texts as a laboratory for looking for what intuition might be. I will stress that these diverse conceptions are mainly formulated in the context of Poincar6＇s controversies in opposition to logicism, to formalism, and in the context of Poincar6＇s very peculiar conventionalism. I will try to demonstrate that, in each case, Poincar~ comes close to a specific tradition （Kant, of course, but also Leibniz and Peirce）.展开更多
The study considers the morphological and physiological behaviour of self-rooted sweet cherry CV （Cultivar） ＇Hedelfinger＇ wild type （H） and somaclonal （HS） grafted on ＇Gisela 6＇ and ＇Colt＇ rootstock. The s...The study considers the morphological and physiological behaviour of self-rooted sweet cherry CV （Cultivar） ＇Hedelfinger＇ wild type （H） and somaclonal （HS） grafted on ＇Gisela 6＇ and ＇Colt＇ rootstock. The somaclonal showed reduced vegetative vigour without any variation of the natural tree＇s architecture. The rootstock ＇Gisela 6＇ caused change in genotype habitus inducing a spreading shape, while ＇Colt＇ increased trunk diameter and height. Fruit quality and size were not affected by genotype nor rootstock. ＇Gisela 6＇, from these preliminary data, had proved the most suitable rootstock for both genotypes since it reduced the tree size and vigor and induced early bearing and the production of a greater number of fruiting spurs.展开更多
Aim The present study aimed to investigate anti-inflammatory activity of 6-AP on murine model of aller- gic contact dermatitis （ACD）. Methods 6＇-acetylpaeoniflorin （6-AP） was synthesized from paeoniflorin （Pae）...Aim The present study aimed to investigate anti-inflammatory activity of 6-AP on murine model of aller- gic contact dermatitis （ACD）. Methods 6＇-acetylpaeoniflorin （6-AP） was synthesized from paeoniflorin （Pae） via acetylation and the structure was characterized by 1H-NMR, 13C-NMR and EI-MS. ACD model was established by repeated application of dinitrochlorobenzene （DNCB） to induce skin immune inflammation. The mice were oral- ly administered 6-AP （35, 70, 140 mg. kg-1 ·d^-l）, Pae （70 rag. kg-1·d^-1） and prednisone （Pre, 5 rag. kg^- 1· d^-1 ） from day 1 to day 7 after Cutaneous inflammation was evaluated by ear swelling and histological exami- nation. Splenocyte proliferation was assayed by the 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyl-2H tetrazolium bromide assay. The cytokine production in the splenocytes was measured by enzyme-linked immunosorbent assay. Results Topical application of DNCB to the skin provoked obvious swelling and inflammatory cell infiltration. 6- AP significantly inhibited ear swelling, decreased inflammatory cell infiltration and epidermal keratinization. Ad- dtionally, 6-AP obviously alleviated the hyperplasia of red pulp and germinal center （GC） appearance, decreased spleen index, decreased spleen index, and inhibited splenocyte proliferation in ACD model, compared to that of Pae. Further, the study indicated that 6-AP treatment could increase IL-10 level, while reduce IL-17 level in splenocytes simultaneously. The correlation analysis displayed significantly positive correlations between IL-17 level and the severity of skin inflammation, while negative correlations between IL-10 level and skin inflammation. Con- clusion 6-AP has a significantly higher anti-inflammatory effect than Pae, and it may be a useful treatment for ACD.展开更多
Aim Paeoniflorin （Pae） is the principal bioactive component of total glucosides of peony （TGP）, which has been widely used in therapy for rheumatoid arthritis （RA）. Paeoniflorin-6＇-O-benzene sulfonate （code： ...Aim Paeoniflorin （Pae） is the principal bioactive component of total glucosides of peony （TGP）, which has been widely used in therapy for rheumatoid arthritis （RA）. Paeoniflorin-6＇-O-benzene sulfonate （code： CP-25） , a novel compound that is a newly ester derivatives of Pae, was evaluated in rats with adjuvant-induced ar- thritis （AA） to study its potential anti-arthritic activity. Methods AA rats were randomly divided into different groups and then treated with CP-25 （25, 50, 100 mg· kg^-1） and methotrexate （0. 5 mg · kg^-1）, from day 16 to day 32 after immunization. Arthritis severity was evaluated by clinical manifestation and histopathological examina- tion. The cells proliferation was determined by CCK-8 assay. Activities of IL-1β, IL-6, IL-17, IL-10, TGF-β1, TNF-oL, IIANKL and OPG were assessed by ELISA. The subsets of CD4 ＋T cells were assayed by flow cytometry. Results CP-25 treatment effectively reduced clinical severity scores and blinded histopathological scores compared with AA groups. CP-25-treated rats exhibited a decrease in the pro-inflammatory cytokines （IL-1β, IL-6, IL-17, and TNF-α） , coupled with an increase in the anti-inflammatory cytokines IL-10 and TGF-β1 in serum and macro- phages of AA rats. The flow cytometry analyses of CD4 ＋T cells dramatically demonstrated the immunomodulatory effects of CP-25 on abnormal immune dysfunction. Apart from the anti-inflammatory activity, treatment with CP-25 inhibited the fibroblast-like synoviocyte （FLS） activation and function. Furthermore, CP-25 treatment of AA rats restored the balance between RANKL and OPG in favor of its anti-osteoclastic effects. Conclusions Data presen- ted here demonstrated that administration of CP-25 significantly inhibited the progression of rat AA, with reductions both in arthritic inflammation and bone damage. The protective effects of CP-25 in AA highlight an attribute that is potential as an ideal new anti-arthritic agent for the treatment with human RA.展开更多
目的:研究溶质载体家族6成员9(solute carrier family 6 member 9,SLC6A9)表达对结直肠癌细胞增殖、迁移和5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:TCGA数据库分析、实时荧光定量PCR和Western blot分析检测SLC6A9在结...目的:研究溶质载体家族6成员9(solute carrier family 6 member 9,SLC6A9)表达对结直肠癌细胞增殖、迁移和5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:TCGA数据库分析、实时荧光定量PCR和Western blot分析检测SLC6A9在结肠癌组织、正常结肠细胞系(NCM460)和结直肠癌细胞系(SW620、HCT116、HT29、Lovo和SW480)中的表达。将SCL6A9过表达质粒及阴性对照(SLC6A9 OE、Vector)转染HT29细胞,将SCL6A9小干扰RNA及阴性对照(SLC6A9 siRNA1#、siRNA2#和Scramble)转染SW620细胞。划痕愈合实验和Transwell实验检测各组细胞的迁移、侵袭能力。Western blot和细胞免疫荧光检测EMT相关蛋白E-cadherin、Vimentin的表达水平。利用CCK-8法和构建裸鼠移植瘤模型检测SLC6A9过表达对结直肠癌细胞5-FU药物敏感性的影响。结果:与正常结肠组织和NCM460细胞相比,SLC6A9在结肠癌组织和结直肠癌细胞系中低表达(均P<0.05)。SLC6A9过表达引起E-cadherin蛋白表达增加,Vimentin蛋白水平降低,抑制结直肠癌细胞的迁移、侵袭(P<0.05)。SLC6A9低表达引起E-cadherin蛋白表达降低,Vimentin蛋白水平增加,促进结直肠癌细胞的迁移、侵袭能力(P<0.05)。SLC6A9过表达提高了5-FU的药物敏感性,并使肿瘤生长缓慢,质量减轻(P<0.05)。而SLC6A9低表达降低了5-FU的药物敏感性(P<0.05)。结论:SLC6A9过表达能够抑制结直肠癌细胞的迁移、侵袭和EMT进程,并增强5-FU对结直肠癌细胞的药物敏感性。展开更多
文摘The aim of this paper is to contribute to the identification and characterization of the various types of intuition put forward by Poincar6, taking his texts as a laboratory for looking for what intuition might be. I will stress that these diverse conceptions are mainly formulated in the context of Poincar6＇s controversies in opposition to logicism, to formalism, and in the context of Poincar6＇s very peculiar conventionalism. I will try to demonstrate that, in each case, Poincar~ comes close to a specific tradition （Kant, of course, but also Leibniz and Peirce）.
文摘The study considers the morphological and physiological behaviour of self-rooted sweet cherry CV （Cultivar） ＇Hedelfinger＇ wild type （H） and somaclonal （HS） grafted on ＇Gisela 6＇ and ＇Colt＇ rootstock. The somaclonal showed reduced vegetative vigour without any variation of the natural tree＇s architecture. The rootstock ＇Gisela 6＇ caused change in genotype habitus inducing a spreading shape, while ＇Colt＇ increased trunk diameter and height. Fruit quality and size were not affected by genotype nor rootstock. ＇Gisela 6＇, from these preliminary data, had proved the most suitable rootstock for both genotypes since it reduced the tree size and vigor and induced early bearing and the production of a greater number of fruiting spurs.
文摘Aim The present study aimed to investigate anti-inflammatory activity of 6-AP on murine model of aller- gic contact dermatitis （ACD）. Methods 6＇-acetylpaeoniflorin （6-AP） was synthesized from paeoniflorin （Pae） via acetylation and the structure was characterized by 1H-NMR, 13C-NMR and EI-MS. ACD model was established by repeated application of dinitrochlorobenzene （DNCB） to induce skin immune inflammation. The mice were oral- ly administered 6-AP （35, 70, 140 mg. kg-1 ·d^-l）, Pae （70 rag. kg-1·d^-1） and prednisone （Pre, 5 rag. kg^- 1· d^-1 ） from day 1 to day 7 after Cutaneous inflammation was evaluated by ear swelling and histological exami- nation. Splenocyte proliferation was assayed by the 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyl-2H tetrazolium bromide assay. The cytokine production in the splenocytes was measured by enzyme-linked immunosorbent assay. Results Topical application of DNCB to the skin provoked obvious swelling and inflammatory cell infiltration. 6- AP significantly inhibited ear swelling, decreased inflammatory cell infiltration and epidermal keratinization. Ad- dtionally, 6-AP obviously alleviated the hyperplasia of red pulp and germinal center （GC） appearance, decreased spleen index, decreased spleen index, and inhibited splenocyte proliferation in ACD model, compared to that of Pae. Further, the study indicated that 6-AP treatment could increase IL-10 level, while reduce IL-17 level in splenocytes simultaneously. The correlation analysis displayed significantly positive correlations between IL-17 level and the severity of skin inflammation, while negative correlations between IL-10 level and skin inflammation. Con- clusion 6-AP has a significantly higher anti-inflammatory effect than Pae, and it may be a useful treatment for ACD.
文摘Aim Paeoniflorin （Pae） is the principal bioactive component of total glucosides of peony （TGP）, which has been widely used in therapy for rheumatoid arthritis （RA）. Paeoniflorin-6＇-O-benzene sulfonate （code： CP-25） , a novel compound that is a newly ester derivatives of Pae, was evaluated in rats with adjuvant-induced ar- thritis （AA） to study its potential anti-arthritic activity. Methods AA rats were randomly divided into different groups and then treated with CP-25 （25, 50, 100 mg· kg^-1） and methotrexate （0. 5 mg · kg^-1）, from day 16 to day 32 after immunization. Arthritis severity was evaluated by clinical manifestation and histopathological examina- tion. The cells proliferation was determined by CCK-8 assay. Activities of IL-1β, IL-6, IL-17, IL-10, TGF-β1, TNF-oL, IIANKL and OPG were assessed by ELISA. The subsets of CD4 ＋T cells were assayed by flow cytometry. Results CP-25 treatment effectively reduced clinical severity scores and blinded histopathological scores compared with AA groups. CP-25-treated rats exhibited a decrease in the pro-inflammatory cytokines （IL-1β, IL-6, IL-17, and TNF-α） , coupled with an increase in the anti-inflammatory cytokines IL-10 and TGF-β1 in serum and macro- phages of AA rats. The flow cytometry analyses of CD4 ＋T cells dramatically demonstrated the immunomodulatory effects of CP-25 on abnormal immune dysfunction. Apart from the anti-inflammatory activity, treatment with CP-25 inhibited the fibroblast-like synoviocyte （FLS） activation and function. Furthermore, CP-25 treatment of AA rats restored the balance between RANKL and OPG in favor of its anti-osteoclastic effects. Conclusions Data presen- ted here demonstrated that administration of CP-25 significantly inhibited the progression of rat AA, with reductions both in arthritic inflammation and bone damage. The protective effects of CP-25 in AA highlight an attribute that is potential as an ideal new anti-arthritic agent for the treatment with human RA.
文摘目的:研究溶质载体家族6成员9(solute carrier family 6 member 9,SLC6A9)表达对结直肠癌细胞增殖、迁移和5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:TCGA数据库分析、实时荧光定量PCR和Western blot分析检测SLC6A9在结肠癌组织、正常结肠细胞系(NCM460)和结直肠癌细胞系(SW620、HCT116、HT29、Lovo和SW480)中的表达。将SCL6A9过表达质粒及阴性对照(SLC6A9 OE、Vector)转染HT29细胞,将SCL6A9小干扰RNA及阴性对照(SLC6A9 siRNA1#、siRNA2#和Scramble)转染SW620细胞。划痕愈合实验和Transwell实验检测各组细胞的迁移、侵袭能力。Western blot和细胞免疫荧光检测EMT相关蛋白E-cadherin、Vimentin的表达水平。利用CCK-8法和构建裸鼠移植瘤模型检测SLC6A9过表达对结直肠癌细胞5-FU药物敏感性的影响。结果:与正常结肠组织和NCM460细胞相比,SLC6A9在结肠癌组织和结直肠癌细胞系中低表达(均P<0.05)。SLC6A9过表达引起E-cadherin蛋白表达增加,Vimentin蛋白水平降低,抑制结直肠癌细胞的迁移、侵袭(P<0.05)。SLC6A9低表达引起E-cadherin蛋白表达降低,Vimentin蛋白水平增加,促进结直肠癌细胞的迁移、侵袭能力(P<0.05)。SLC6A9过表达提高了5-FU的药物敏感性,并使肿瘤生长缓慢,质量减轻(P<0.05)。而SLC6A9低表达降低了5-FU的药物敏感性(P<0.05)。结论:SLC6A9过表达能够抑制结直肠癌细胞的迁移、侵袭和EMT进程,并增强5-FU对结直肠癌细胞的药物敏感性。
