Hydrothermal Syntheses, Crystal Structures and Luminescence Properties of Cu(Ⅱ) and Cd(Ⅱ) Complexes Assembled by 6-Hydroxypicolinic Acid and 1,10-Phenanthroline

Two new complexes based on 6-hydroxypicolinic acid(H_2picO) and 1,10-phenanthroline(phen), such as [Cu_4(picO)_4(phen)_4]·12 H_2O(1) and {[Cd_4(picO)_4(phen)_4(H_2O)_2]·5H_2O}_n(2), have been synthesized and characterized by IR, elemental analyses, thermogravimetric analyses and X-ray diffraction technique. Single-crystal X-ray diffraction analyses revealed that the two complexes both crystallize in the triclinic system, space group P(16)-. For complex 1, the picO ligands adopt a tridentate coordination mode to link copper(Ⅱ) ions into dimmers. PicO ligands also adopt a tridentate mode in complex 2, which connects the cadmium(Ⅱ) to form a one-dimensional chain. Moreover, luminescent properties of 1 and 2 were also investigated.

Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

BACKGROUND Leukemia stem cells(LSCs)are found to be one of the main factors contributing to poor therapeutic effects in acute myeloid leukemia(AML),as they are protected by the bone marrow microenvironment(BMM)against conventional therapies.Gossypol acetic acid(GAA),which is extracted from the seeds of cotton plants,exerts anti-tumor roles in several types of cancer and has been reported to induce apoptosis of LSCs by inhibiting Bcl2.AIM To investigate the exact roles of GAA in regulating LSCs under different microenvironments and the exact mechanism.METHODS In this study,LSCs were magnetically sorted from AML cell lines and the CD34+CD38-population was obtained.The expression of leucine-rich pentatricopeptide repeat-containing protein(LRPPRC)and forkhead box M1(FOXM1)was evaluated in LSCs,and the effects of GAA on malignancies and mitochondrial RESULTS LRPPRC was found to be upregulated,and GAA inhibited cell proliferation by degrading LRPPRC.GAA induced LRPPRC degradation and inhibited the activation of interleukin 6(IL-6)/janus kinase(JAK)1/signal transducer and activator of transcription(STAT)3 signaling,enhancing chemosensitivity in LSCs against conventional chemotherapies,including L-Asparaginase,Dexamethasone,and cytarabine.GAA was also found to downregulate FOXM1 indirectly by regulating LRPPRC.Furthermore,GAA induced reactive oxygen species accumulation,disturbed mitochondrial homeostasis,and caused mitochondrial dysfunction.By inhibiting IL-6/JAK1/STAT3 signaling via degrading LRPPRC,GAA resulted in the elimination of LSCs.Meanwhile,GAA induced oxidative stress and subsequent cell damage by causing mitochondrial damage.CONCLUSION Taken together,the results indicate that GAA might overcome the BMM protective effect and be considered as a novel and effective combination therapy for AML.

非小细胞肺癌组织中circBIRC6、APPBP2表达及临床预后意义

目的 探讨非小细胞肺癌(NSCLC)组织中环状核糖核酸杆状病毒IAP重复序列6(circBIRC6)、β淀粉样蛋白前体蛋白结合蛋白2(APPBP2)表达及临床预后意义。方法 收集2018年6月~2020年1月90例在河北北方学院附属第一医院行手术切除的NSCLC组织及癌旁组织,采用实时荧光定量聚合酶链式反应检测circBIRC6、APPBP2表达,并分析二者与NSCLC患者临床病理特征的关系。通过Pearson相关性分析NSCLC组织中circBIRC6与APPBP2 mRNA表达的相关性,Kaplan-Meier法绘制不同表皮生长因子受体(EGFR)基因突变、TNM分期和circBIRC6、APPBP2 mRNA表达的NSCLC患者生存曲线,多因素Cox回归分析NSCLC患者预后的影响因素。结果 与癌旁组织比较,NSCLC组织中circBIRC6、APPBP2 mRNA表达升高(P<0.05)。NSCLC组织中circBIRC6与APPBP2 mRNA表达呈正相关(r=0.817,P<0.001)。NSCLC患者不同分化程度、TNM分期、淋巴结转移组织中circBIRC6、APPBP2 mRNA表达比较有差异(P<0.05)。随访3年,90例NSCLC患者总生存率为55.56%(50/90)。EGFR基因突变阳性/阴性NSCLC患者总生存率比较无差异(P>0.05);TNM分期Ⅰ~Ⅱ期NSCLC患者总生存率高于Ⅲ期NSCLC患者(P<0.05);circBIRC6、APPBP2 mRNA高表达组生存率低于circBIRC6、APPBP2 mRNA低表达组(P<0.05)。低分化、TNM分期Ⅲ期、淋巴结转移和circBIRC6≥10.97、APPBP2 mRNA≥2.48为NSCLC患者死亡的独立危险因素[OR(95%CI)=3.586(1.080~11.909)、3.632(1.193~11.057)、3.197(1.060~9.640)、3.223(1.086~9.570)、2.767(1.022~7.492)]。结论 NSCLC组织中circBIRC6、APPBP2 mRNA高表达,与分化程度、TNM分期、淋巴结转移和预后有关。

齐刺环跳穴干预坐骨神经损伤大鼠海马IL-1β、IL-6、TNF-α及GFAP表达影响的实验研究

目的 通过观察齐刺环跳穴对坐骨神经损伤大鼠海马白细胞介素-1β(Interleukin-1β,IL-1β)、白细胞介素-6(Interleukin-6,IL-6)、肿瘤坏死因子-α(Tumor Necrosis Factor-alpha, TNF-α)及胶质纤维酸性蛋白(Glial Fibrillary Acidic Protein, GFAP)表达的影响,探讨齐刺环跳穴治疗坐骨神经痛的中枢镇痛机制。方法 60只SD大鼠随机分为假手术组、模型组、齐刺组、单刺组及药物组,每组12只,采用钳夹法制备大鼠坐骨神经损伤模型。造模第2天开始进行针刺及药物干预,连续14 d。观察各组大鼠干预前后坐骨神经功能指数(Sciatic nerve Function Index, SFI)、热缩足反射潜伏期(Paw Withdrawal Latency, PWL)的变化,ELISA法检测海马组织IL-1β、IL-6、TNF-α蛋白表达水平,实时定量PCR法及免疫组化法检测海马组织GFAP表达水平。结果 干预前,与假手术组比较,其余各组大鼠PWL值、SPI值显著降低(P<0.01),与模型组比较,齐刺组干预后大鼠PWL值、SPI值显著改善(P<0.01)。ELISA法、RT-PCR法及免疫组化检测结果显示,模型组、齐刺组、单刺组、药物组IL-1β、IL-6、TNF-α、GFAP表达较假手术组显著升高(P<0.01);与模型组相比,齐刺组、单刺组、药物组IL-1β、IL-6、TNF-α、GFAP表达显著下降,齐刺组表达低于单刺组及药物组(P<0.01)。结论 齐刺环跳穴缓解坐骨神经痛的镇痛机制可能与下调海马炎症因子表达,抑制星形胶质细胞活化,从而降低中枢痛觉敏化程度有关。

Different n-6/n-3 polyunsaturated fatty acid ratios affect postprandial metabolism in normal and hypertriglyceridemic rats

Postprandial metabolism plays major roles in many pathological conditions.The n-6/n-3 polyunsaturated fatty acid(PUFA)ratio is closely related to various physiological disorders.This study aimed to investigate the effects of high fat meals with different n-6/n-3 PUFA ratios on postprandial metabolism in normal control(NC)and hypertriglyceridemia(HTG)rats.The postprandial response of triglyceride(TG)in HTG groups was higher than that in NC groups after different n-6/n-3 PUFA ratio meals.The HTG groups showed higher postprandial total cholesterol(TC)responses than NC groups after 1:1 and 20:1 ratio meals.The 5:1 n-6/n-3 PUFA ratio elicited lower postprandial responses of tumor necrosis factorα(TNF-α)than 1:1 and 10:1 ratios in HTG groups.The postprandial malondialdehyde(MDA)response was lower after a 5:1 n-6/n-3 PUFA ratio meal than 1:1 and 20:1 ratio meals in HTG groups.The 1:1 ratio resulted in a lower postprandial reactive oxygen species(ROS)level than 5:1 and 10:1 n-6/n-3 PUFA ratios in NC groups.The results showed that a low n-6/n-3 PUFA ratio improved postprandial dysmetabolism induced by a high fat meal in NC and HTG rats.A high n-6/n-3 PUFA ratio increased the difference in postprandial metabolism between NC and HTG rats.

Ellagic acid ameliorates cisplatin-induced acute kidney injury by regulating inflammation and SIRT6/TNF-α signaling

De spite cisplatin has been widely used in the treatment of various cancers,the noteworthy nephrotoxicity greatly constrained its clinical value.For this reason,finding novel targeted therapies to attenuate the nephrotoxicity of cisplatin should be pretty significant.Our previous study found that histone deacetylase sirtuin 6(SIRT6)could be an ideal target for the treatment of cisplatin-induced acute kidney injury.In this study,we explored the protective effects of ellagic acid,a natural polyphenol compound that activates SIRT6,on cisplatin-induced nephrotoxicity.Pre-treatment of ellagic acid attenuated cytotoxicity of cisplatin in primary renal cells and TCMK-1 cells.Moreover,ellagic acid ameliorated renal dysfunction,apoptosis and fibrosis induced by cisplatin in mice.Furthermore,ellagic acid reduced nephrotoxicity-associated inflammatory factor interleukin(IL)-1βand IL-6 expression both in vitro and in vivo.Mechanistically,ellagic acid reversed cisplatin-reduced SIRT6 expression and diminished cisplatin-induced tumor necrosis factor(TNF)-αexpression.And SIRT6 knockdown abrogated the protective effects of ellagic acid on cisplatin-induced cell apoptosis,indicating the renal-protective effects of ellagic acid are mainly dependent on ellagic acid-mediated SIRT6 activation.Our results provide preclinical rationale for using ellagic acid as a feasible and promising agent to ameliorate cisplatin-induced acute kidney injury,and support ellagic acid as a potential adjunctive therapy for future cancer treatment.

敲低PRDX6对利福平诱导HepG2细胞胆汁酸转运体适应性表达的影响

目的探讨敲低过氧化物还原酶-6(PRDX6)在利福平(RFP)诱导人肝癌细胞(HepG2)损伤及胆汁酸转运体适应性表达中的作用。方法将处于对数生长期的细胞均匀接种于6孔板中,使用特异PRDX6-siRNA、control-siRNA分别转染HepG2细胞构建敲低组及对照组。给予细胞100μmol/L RFP诱导24 h后,Western blot和qRT-PCR检测各组细胞PRDX6、多药耐药蛋白1(MDR1)、多药耐药相关蛋白2、3、4(MRP2、MRP3、MRP4)、Na+/牛磺胆酸协同转运蛋白(NTCP)的蛋白及基因表达水平;Annexin V-FITC/PI双染法检测各组细胞凋亡率;CCK-8法检测各组细胞增殖变化;试剂盒检测各组细胞培养上清液中细胞损伤标志物丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、总胆汁酸(TBA)相对含量变化。结果RFP可诱导HepG2细胞MRP2、MRP3、MRP4、MDR1、NTCP及PRDX6的蛋白和基因表达水平升高(P<0.05),而敲低PRDX6后,MRP2、MRP3、MRP4、MDR1、NTCP的蛋白和基因表达水平均有不同程度的降低(P<0.05)。此外,PRDX6敲低后HepG2细胞凋亡率升高(P<0.05),细胞增殖能力下降(P<0.05),细胞培养上清液中细胞损伤标志物(ALT、AST、TBIL、DBIL、TBA)水平升高(P<0.05)。结论RFP可增加HepG2细胞胆汁酸转运体及PRDX6的蛋白和基因表达量,敲低PRDX6并用RFP诱导后胆汁酸转运体的蛋白及基因表达量降低,同时细胞损伤加重,表明PRDX6在RFP诱导的HepG2细胞适应性反应中发挥保护作用。

Betulinic acid-mediating miRNA-365 inhibited the progression of pancreatic cancer

Background:The dilemma of pancreatic cancer treatment has become a global challenge.For this reason,effective,feasible,and new medical methods are currently much-needed.Betulinic acid(BA)has been valued as a potential therapy for pancreatic cancer.However,the mechanism by which BA exerts an inhibitory effect on the development of pancreatic cancer remains elusive.Methods:A rat model and two cell models of pancreatic cancer were established,and the effect of BA on pancreatic cancer was verified in vivo and in vitro by using MTT,Transwell,flow cytometry,RT-PCR,Elisa and immunohistochemistry.At the same time,miR-365 inhibitors were introduced to test whether BA played a role in mediating miR-365.Results:BA can significantly inhibit the proliferation and invasion of pancreatic cancer cells and promote apoptosis.In vivo experiments,BA can significantly lower the number of cancer cells and tumor volume in the rat model of pancreatic cancer.In vitro,it was found that BA inhibited the protein level and phosphorylation level of AKT/STAT3 by mediating the expression of miR365/BTG2/IL-6.Like BA,miR-365 inhibitors also significantly inhibited cell viability and invasion ability,and inhibited the protein level and phosphorylation level of AKT/STAT3 by changing the expression of BTG2/IL-6,and their combination had a synergistic effect.Conclusion:BA inhibits AKT/STAT3 expression and phosphorylation by modulating miR-365/BTG2/IL-6 expression,and BA inhibits the progression of pancreatic cancer through the aforementioned mechanism.

Silencing of the Nonspecific Phospholipase C6(NPC6)Gene Induces Ricinoleic Acid Accumulation in Castor Seeds

Castor,scientifically known as Ricinus communis L.,is among the top ten oil crops globally.It is considered a renewable resource and is commonly referred to as‘green oil’.Castor seeds contain castor oil as their main component,which is predominantly composed of ricinoleic acid.This study utilized RNAi technology to silence the NPC6 gene in NO.2129 castor,resulting in the creation of mutant plants L1 and L2.The weight of 100 dry seed kernels from L1 and L2 exceeds that from NO.2129.The crude fat and ricinoleic acid levels of L1 and L2 were higher than those of NO.2129 at various developmental stages.In the proteomics analysis of 60-day-old castor seeds,a total of 21 differentially expressed proteins were identified,out of which 19 were successfully recognized.Eleven of the differentially expressed proteins identified were legumins,which play a crucial role in nutrient storage within the seed.Silencing the NPC6 gene results in the accumulation of ricinoleic acid in castor seeds.The findings of this study not only enhance our knowledge of NPC6’s role in regulating castor seed oil synthesis but also offer fresh perspectives for investigating oil synthesis and accumulation in other plant species.

Q[6]-PW_(12)自组装体制备及其催化合成乙酸乙酯

以六元瓜环(Q[6])及Keggin型磷钨酸(PW_(12))为原料,制备Q[6]-PW_(12)自组装体。将Q[6]-PW_(12)自组装体作为催化剂应用于乙酸与乙醇酯化反应合成乙酸乙酯。结果表明,Q[6]与PW_(12)发生了自组装作用,且Q[6]-PW_(12)自组装体具有多孔结构特征;Q[6]-PW_(12)自组装体对酯化反应合成乙酸乙酯具有催化作用,且自组装体催化剂经过多次循环使用后依然效果良好。

血清ADMA、IL-6、内毒素及乳酸在新生儿败血症所致感染性休克中的表达及临床意义

目的探讨血清不对称二甲基精氨酸(ADMA)、白细胞介素-6(IL-6)、内毒素及乳酸在新生儿败血症所致感染性休克中的表达及临床意义。方法选取我院收治的58例新生儿败血症所致感染性休克患儿为休克组,62例新生儿败血症未发生感染性休克患儿为败血症组,60例无感染新生儿为对照组。检测所有研究对象血清中的ADMA、IL-6、内毒素及乳酸水平。比较三组研究对象的ADMA、IL-6、内毒素及乳酸水平;分析ADMA、IL-6、内毒素及乳酸水平对新生儿败血症所致感染性休克患儿的诊断价值;分析ADMA水平与IL-6、内毒素及乳酸水平的关系;分析ADMA、IL-6、内毒素及乳酸水平与休克组预后的关系。结果休克组的ADMA、IL-6、内毒素及乳酸水平均高于败血症组及对照组(P<0.05);败血症组的ADMA、IL-6、内毒素水平均高于对照组(P<0.05);败血症组和对照组的乳酸水平无显著差异(P>0.05)。受试者工作特征曲线(ROC)结果显示,ADMA、IL-6、内毒素及乳酸诊断新生儿败血症所致感染性休克的曲线下面积(AUC)分别为0.93、0.89、0.89、0.92。Pearson相关性分析结果显示,ADMA水平与IL-6、内毒素及乳酸水平呈正相关(P<0.05)。ADMA、IL-6、内毒素及乳酸高水平与新生儿败血症所致感染性休克预后不良有关。结论血清ADMA、IL-6、内毒素及乳酸在新生儿败血症所致感染性休克中表达水平较高,可作为该疾病诊断的生物标志物并预测预后。

基于IL-6/STAT3信号通路探讨山楂酸对小鼠结肠癌CT26细胞增殖、凋亡的影响

目的从IL-6/STAT3信号通路探讨山楂酸(MA)对小鼠结肠癌CT26细胞增殖、凋亡的影响。方法体外培养CT26细胞,设置空白组、10、20、30、35、40μmoL·L^(-1)MA组。干预24 h后,采用CCK-8法检测山楂酸对细胞活力的影响,采用RT-PCR检测IL-6 mRNA表达水平,采用流式细胞仪检测各组细胞凋亡率,采用Western blotting检测各组细胞中STAT3通路相关蛋白的表达水平。设置空白组、IL-6(20 ng·μL^(-1))刺激组、不同浓度MA与IL-6共刺激组,采用Western blotting检测各组细胞p-STAT3、STAT3蛋白的表达水平。设置空白组、MA组、磷酸酶抑制剂(正钒酸钠)组和MA+正钒酸钠组,采用Western blotting检测各组细胞p-STAT3、STAT3蛋白的表达水平。结果与空白组相比,CCK-8结果显示20、30、35、40、45、50μmoL·L^(-1)浓度的山楂酸均能降低CT26细胞活力(P<0.05),IC50=37.32μmoL·L^(-1);RT-PCR结果显示30、35、40μmoL·L^(-1)给药组细胞中IL-6 mRNA水平下降(P<0.05);流式细胞术结果显示30、35、40μmoL·L^(-1)给药组细胞凋亡率增加(P<0.05);Western blotting结果显示35、40μmoL·L^(-1)给药组细胞中STAT3通路相关蛋白p-STAT3、Bcl-2蛋白表达水平下降(P<0.05),MA+正钒酸钠组能恢复MA单独给药组中被降低的p-STAT3蛋白水平(P<0.05)。结论MA可能通过降低IL-6的表达水平,抑制STAT3的磷酸化,从而降低抗凋亡蛋白Bcl-2的表达,促进肿瘤细胞凋亡,抑制其增殖。

生物质植酸改性聚氨酯涂层锦纶6织物的制备及其阻燃性能

针对聚氨酯涂层锦纶6织物阻燃性能差、熔滴严重的问题,通过采用生物质植酸对水性聚氨酯进行改性,开发环保高效的阻燃体系对锦纶6织物进行涂层改性。测试分析了涂层锦纶6织物的阻燃性能、耐水洗性能、耐静水压、热稳定性和阻燃机制等。研究结果表明:聚氨酯涂层锦纶6织物在垂直燃烧测试中完全燃烧,损毁长度为30 cm,熔滴引燃脱脂棉,植酸/植酸钠改性聚氨酯涂层锦纶6织物在垂直燃烧中能够自熄,无熔滴产生,损毁长度降低至12.4 cm,极限氧指数升高至29.0%;经过10次水洗后涂层锦纶6织物仍能自熄,具有较好的阻燃性能和耐水洗性能;涂层锦纶6织物的热分解提前,经热分解后磷元素保留在残炭中,参与成炭,植酸盐主要通过固相阻燃机制提高涂层锦纶6织物的阻燃性能。

Additive neurorestorative effects of exercise and docosahexaenoic acid intake in a mouse model of Parkinson’s disease

There is a need to develop interventions to slow or reverse the degeneration of dopamine neurons in Parkinson’s disease after diagnosis.Given that preclinical and clinical studies suggest benefits of dietary n-3 polyunsaturated fatty acids,such as docosahexaenoic acid,and exercise in Parkinson’s disease,we investigated whether both could synergistically interact to induce recovery of the dopaminergic pathway.First,mice received a unilateral stereotactic injection of 6-hydroxydopamine into the striatum to establish an animal model of nigrostriatal denervation.Four weeks after lesion,animals were fed a docosahexaenoic acid-enriched or a control diet for the next 8 weeks.During this period,the animals had access to a running wheel,which they could use or not.Docosahexaenoic acid treatment,voluntary exercise,or the combination of both had no effect on(i)distance traveled in the open field test,(ii)the percentage of contraversive rotations in the apomorphine-induction test or(iii)the number of tyrosine-hydroxylase-positive cells in the substantia nigra pars compacta.However,the docosahexaenoic acid diet increased the number of tyrosine-hydroxylase-positive terminals and induced a rise in dopamine concentrations in the lesioned striatum.Compared to docosahexaenoic acid treatment or exercise alone,the combination of docosahexaenoic acid and exercise(i)improved forelimb balance in the stepping test,(ii)decreased the striatal DOPAC/dopamine ratio and(iii)led to increased dopamine transporter levels in the lesioned striatum.The present results suggest that the combination of exercise and docosahexaenoic acid may act synergistically in the striatum of mice with a unilateral lesion of the dopaminergic system and provide support for clinical trials combining nutrition and physical exercise in the treatment of Parkinson’s disease.

6-苄基氨基嘌呤结合柠檬酸复合处理对水晶葡萄采后品质的影响

为探究柠檬酸、6-苄基氨基嘌呤(6-benzylaminopurine,6-BA)及复合处理对水晶葡萄采后品质的影响,分别使用30 mg/L 6-BA、1%柠檬酸及两者复合液对水晶葡萄进行浸泡处理后贮藏于(1±0.3)℃,85%~90%相对湿度保鲜库内40 d,每10 d对各项指标进行测定。结果表明,复合处理能有效抑制水晶葡萄腐烂率、褐变率及脱落率的增加,减缓硬度、多酚、维生素C及可溶性固形物含量的下降和多酚氧化酶(polyphenol oxidase,PPO)活性的升高,保持可溶性蛋白质和游离氨基酸含量接近采摘当天时的含量,使水晶葡萄采后保持良好的感官和营养品质。贮藏第40天时,复合处理组果实腐烂率、褐变率及PPO活性分别比对照组低51.01%、48.94%、36.80%,而果实硬度、维生素C含量、多酚含量分别是对照组的1.42倍、2.05倍、2.91倍,显著延缓了水晶葡萄软化和褐变。因此,6-BA和柠檬酸复合处理更有利于维持水晶葡萄贮藏期间的综合品质。

铜改性抗菌防螨聚酰胺6纤维的制备及其性能

为解决纳米铜抗菌剂在纤维中分散性、界面相容性差的问题,采用油酸对纳米球形铜抗菌剂进行包覆处理,并与聚酰胺6(PA6)基体共混挤出造粒制得抗菌防螨PA6切片,再经熔融单组分纺丝和熔融复合纺丝制得铜改性抗菌防螨PA6纤维。对铜抗菌剂的形貌结构和界面相容性,抗菌防螨PA6切片的热稳定性和可纺性以及纤维的铜含量、力学性能和抗菌防霉防螨性能进行分析。结果表明:油酸包覆球形铜抗菌剂的分散性较好,且与PA6基体相容性良好,抗菌防螨PA6切片的热稳定性、可纺性良好;铜改性抗菌防螨PA6纤维的颜色均匀性一致,纤维制成率高达88%,铜改性PA6拉伸变形丝的断裂伸长率为29.95%,断裂强度达到4.43 cN/dtex;洗涤50次前后铜改性抗菌防螨PA6纤维对白色念珠菌、金黄色葡萄球菌、大肠杆菌的抑菌率均大于99%,其织物防霉等级达到0级,螨虫驱避率达到89%,抗菌防霉防螨性能高效耐久。

m^(6)A-脂肪酸代谢相关lncRNA对结肠癌预后及免疫疗效的预测价值

目的 探讨结肠癌中m^(6)A-脂肪酸代谢相关lncRNA,并分析其与患者预后及肿瘤免疫微环境的相关性,为结肠癌患者诊治提供新的分子靶点。方法 从TCGA数据库中获取结肠癌患者的转录组数据及临床信息。通过Pearson相关分析筛选出m^(6)A-脂肪酸代谢相关lncRNA。采用单因素、LASSO及多因素Cox回归分析构建基于lncRNA表达的风险模型;通过Kaplan-Meier生存曲线、ROC曲线及列线图验证风险模型的准确性。比较高风险组及低风险组结肠癌患者免疫细胞浸润情况。结果 从TCGA数据库中鉴定出670个m^(6)A相关lncRNA及1 011个脂肪酸代谢相关lncRNA,其中39个交叉的lncRNA具有预后意义。通过LASSO及多因素回归构建了包含5个m^(6)A-脂肪酸代谢相关lncRNA的风险模型,风险评分较高者预后较差。多因素Cox回归分析表明风险评分是影响患者预后的独立相关因素(HR=11.8,95%CI 3.6~38.7)。ROC曲线显示其预测患者1年、3年、5年生存率的曲线下面积分别为0.758 (95%CI 0.611~0.905)、0.793 (95%CI 0.708~0.877)和0.815 (95%CI 0.722~0.907)。相较于高风险组,低风险组肿瘤有更多的M1型巨噬细胞浸润(P<0.05),免疫检查点基因(CD44、TNFRSF9、CD40LG、CD48、CD244、IDO1、HAVCR2、CD27、ICOS、LAIR1、TMIGD2、CD28、TIGIT)表达增高(P<0.05)。结论 该m^(6)A-脂肪酸代谢相关lncRNA风险模型可用于预测结肠癌患者预后及免疫疗效。

氨基酸表面活性剂及多元醇对月桂醇聚醚-6羧酸钠泡沫性能的影响

以月桂醇聚醚-6羧酸钠为研究对象,考察了复配不同种类的氨基酸表面活性剂及不同多元醇对其泡沫性能的影响。结果表明:月桂酰肌氨酸钠、椰油酰氨基丙酸钠与月桂醇聚醚-6羧酸钠复配具有较好的发泡力,而椰油酰谷氨酸二钠更有利于提升月桂醇聚醚-6羧酸钠的泡沫稳定性。在配方稳定性上,复配10%的月桂酰肌氨酸钠即可改善月桂醇聚醚-6羧酸钠的低温稳定性,使得样品在低温下仍为透明澄清液体。此外,不同多元醇的添加不会影响月桂醇聚醚-6羧酸钠的发泡力,而在泡沫稳定性上,添加甘油、麦芽糖醇则优于甲基丙二醇、聚乙二醇-8、丙二醇。

n-6多不饱和脂肪酸生理功能及其在肉牛生产中的应用

n-6多不饱和脂肪酸是动物的必需脂肪酸,与机体脂代谢、免疫机能和繁殖性能密切相关。研究发现,饲粮补充n-6多不饱和脂肪酸不仅可促进母牛妊娠建立而提高受胎率,且有利于胎儿发育、子代健康和生长发育,并改善育肥牛生产性能和胴体品质。鉴于此,本文综述了n-6多不饱和脂肪酸的生理功能,并详细阐述其在肉牛生产中的应用,为生产上科学合理地应用n-6多不饱和脂肪酸改善肉牛繁殖机能、提升健康和生产性能提供理论基础。

Gallic acid与DA-6强化黑麦草修复复合重金属(Cd、Pb、Cu、Zn)污染土壤的研究

用盆栽实验研究了Gallic acid与DA-6单独及耦合使用对黑麦草修复复合重金属污染土壤的影响。结果表明,Gallic acid与DA-6单独及耦合使用,能使黑麦草对重金属污染土壤中Cd、Zn的修复产生明显的促进作用,但对Pb、Cu的修复没有明显变化。当添加5 mmol/kg的Gallic acid时,黑麦草对Cd、Zn的富集量分别达到了15.9 mg/kg与1 660.8 mg/kg,富集系数较未添加Gallic acid时提高了93.9%,233.9%;同时添加10μmol/kg DA-6与5 mmol/kg Gallic Acid之后,黑麦草对Cd、Zn的富集量分别达到了18.8 mg/kg,1 680.5 mg/kg,富集系数较未添加DA-6时提高了18.2%与1.2%。