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Differential Expression of Retrotransposon WIS 2-1A Response to Vacuum, Low-Energy N^+ Implantation and ^(60)Coγ-ray Irradiation in Wheat
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作者 赵慧茹 谷运红 +2 位作者 押辉远 焦浈 秦广雍 《Plasma Science and Technology》 SCIE EI CAS CSCD 2009年第1期104-109,共6页
Mutagenesis and retrotransposons have a close relationship, but little attention has been paid yet to the activity of retrotransposons produced by physical mutagens. The variation of retrotransposon WIS 2-1A activity ... Mutagenesis and retrotransposons have a close relationship, but little attention has been paid yet to the activity of retrotransposons produced by physical mutagens. The variation of retrotransposon WIS 2-1A activity in wheat (Triticum aestivum L.) embryos at three different growth times (30 h, 45 h and 60 h) was investigated after they had been treated with N^+ implantation in a vacuum of 5× 10^-2 Pa and irradiation by ^60Coγ-ray respectively. For each of the three growth times the expression of WIS 2-1A showed almost entirely a same trend of downregulation, upregulation, then downregulation, and upregulation again with the increase in dose of N^+ implantation, but the expression appeared irregular with the increase in irradiation of ^60Coγ-ray. In conclusion, the acutely activating effect of WIS 2-1A stimulated by vacuum and high dose N^+ implantation within a shorter incubation time may provide a convenient tool to advance the research on mutagenic breeding and function genes. 展开更多
关键词 RETROTRANSPOSON WIS 2-1A transcription activity VACUUM N^+ implantation ^60coγ-ray irradiation
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