Remaining true to our original aspiration, PetroChina Research Institute of Petroleum Exploration and Development (RIPED) has been forging ahead, going through a glorious development course of 60 years.Here, let s ext...Remaining true to our original aspiration, PetroChina Research Institute of Petroleum Exploration and Development (RIPED) has been forging ahead, going through a glorious development course of 60 years.Here, let s extend our warmest congratulations.Over the past six decades, generations of petroleum science and technology workers from the展开更多
T he jubilee60th MSV(International Engineering Fair)has showcased cutting-edge technology and the best of the history and present of the Czech and Slovak industry.From1to5October2018,six industrial fairs were held at ...T he jubilee60th MSV(International Engineering Fair)has showcased cutting-edge technology and the best of the history and present of the Czech and Slovak industry.From1to5October2018,six industrial fairs were held at the Brno Exhibition Centre,together with a unique exhibition commemorating the100th anniversary of Czechoslovakia.展开更多
Remaining true to our original aspiration, PetroChina Research Institute of Petroleum Exploration and Development (RIPED) has been forging ahead, going through a glorious development course of 60 years.Here, let s ext...Remaining true to our original aspiration, PetroChina Research Institute of Petroleum Exploration and Development (RIPED) has been forging ahead, going through a glorious development course of 60 years.Here, let s extend, our warmest congratulations.展开更多
It is our great pleasure to put up this special issue in Journal of Control Theory and Applications in honor of the 60th birthday of Professor Frank Lewis, who has made many significant contributions to the field of c...It is our great pleasure to put up this special issue in Journal of Control Theory and Applications in honor of the 60th birthday of Professor Frank Lewis, who has made many significant contributions to the field of control engineering through the years, and who is regarded as a pioneer in many areas in control and automation, which includes applied optimal control and geometric systems theory in his earlier research period, and his recent focus on intelligent nonlinear control,展开更多
To commemorate the 60th anniversary of democratic reform in Tibet, the photo exhibition named'Milestone of Abolishing the Feudal Serfdom-Exhibition for the 60th Anniversary of Democratic Reform in Tibet'was op...To commemorate the 60th anniversary of democratic reform in Tibet, the photo exhibition named'Milestone of Abolishing the Feudal Serfdom-Exhibition for the 60th Anniversary of Democratic Reform in Tibet'was opened on March 29th, 2019at the Tibet Cultural Museum in Beijing.By nearly 400 pictures.展开更多
Objective:To evaluate the immunostimulatory potential of crossreactive molecule heat shock protein 60(HSP60)of filarial parasite Brugia malayi and Leishmania donovani.Methods:HSP60 of Brugia malayi(BmHSP60)was amplifi...Objective:To evaluate the immunostimulatory potential of crossreactive molecule heat shock protein 60(HSP60)of filarial parasite Brugia malayi and Leishmania donovani.Methods:HSP60 of Brugia malayi(BmHSP60)was amplified using gene-specific primer,cloned in p Tri Ex4 vector,expressed in BL21-DE3 cells,and recombinant HSP60(rHSP60)of~65 k Da was purified by affinity chromatography using Ni-NTA column.The recombinant protein was desalted by the dialysis membrane,and the presence of endotoxin level was determined by Limulus amebocyte lysate assay.The recombinant protein was tested for cell proliferation,nitric oxide release,expression of Th1 and Th2 cytokines,and transcription factors(STATs)in vitro using murine macrophage cell line(J774 A.1).Results:Higher cell proliferation indicated that BmHSP60 had immunostimulatory potential.rBmHSP60 exposure upregulated the expression of iNOS,STAT1,STAT4,Th1 cytokines(IFN-γ,TNF-α,IL-12),and nitric oxide release.In addition,no remarkable change was observed in the expression of IL-6,IL-10,and STAT3 in macrophage cell line J774 A.1.The ELISA analysis showed the levels of IFN-γ,TNF-α,and IL-12 were upregulated while IL-10 level was downregulated,revealing that BmHSP60 triggered a Th1 immune response.Conclusions:Our study demonstrates that rBmHSP60 has immunogenic properties which effectively enhances the Th1 type immune responses,and can be used as an immunoprophylactic agent against leishmaniasis.Furthermore,in vivo studies are in progress to determine the protective role of rBmHSP60 against Leishmania donovani infection in a mouse model.展开更多
文摘Remaining true to our original aspiration, PetroChina Research Institute of Petroleum Exploration and Development (RIPED) has been forging ahead, going through a glorious development course of 60 years.Here, let s extend our warmest congratulations.Over the past six decades, generations of petroleum science and technology workers from the
文摘T he jubilee60th MSV(International Engineering Fair)has showcased cutting-edge technology and the best of the history and present of the Czech and Slovak industry.From1to5October2018,six industrial fairs were held at the Brno Exhibition Centre,together with a unique exhibition commemorating the100th anniversary of Czechoslovakia.
文摘Remaining true to our original aspiration, PetroChina Research Institute of Petroleum Exploration and Development (RIPED) has been forging ahead, going through a glorious development course of 60 years.Here, let s extend, our warmest congratulations.
文摘It is our great pleasure to put up this special issue in Journal of Control Theory and Applications in honor of the 60th birthday of Professor Frank Lewis, who has made many significant contributions to the field of control engineering through the years, and who is regarded as a pioneer in many areas in control and automation, which includes applied optimal control and geometric systems theory in his earlier research period, and his recent focus on intelligent nonlinear control,
文摘To commemorate the 60th anniversary of democratic reform in Tibet, the photo exhibition named'Milestone of Abolishing the Feudal Serfdom-Exhibition for the 60th Anniversary of Democratic Reform in Tibet'was opened on March 29th, 2019at the Tibet Cultural Museum in Beijing.By nearly 400 pictures.
基金supported by Department of Science and Technology,Science and Engineering Research Board,New Delhi,India(N-PDF Project No PDF/2016/001487)
文摘Objective:To evaluate the immunostimulatory potential of crossreactive molecule heat shock protein 60(HSP60)of filarial parasite Brugia malayi and Leishmania donovani.Methods:HSP60 of Brugia malayi(BmHSP60)was amplified using gene-specific primer,cloned in p Tri Ex4 vector,expressed in BL21-DE3 cells,and recombinant HSP60(rHSP60)of~65 k Da was purified by affinity chromatography using Ni-NTA column.The recombinant protein was desalted by the dialysis membrane,and the presence of endotoxin level was determined by Limulus amebocyte lysate assay.The recombinant protein was tested for cell proliferation,nitric oxide release,expression of Th1 and Th2 cytokines,and transcription factors(STATs)in vitro using murine macrophage cell line(J774 A.1).Results:Higher cell proliferation indicated that BmHSP60 had immunostimulatory potential.rBmHSP60 exposure upregulated the expression of iNOS,STAT1,STAT4,Th1 cytokines(IFN-γ,TNF-α,IL-12),and nitric oxide release.In addition,no remarkable change was observed in the expression of IL-6,IL-10,and STAT3 in macrophage cell line J774 A.1.The ELISA analysis showed the levels of IFN-γ,TNF-α,and IL-12 were upregulated while IL-10 level was downregulated,revealing that BmHSP60 triggered a Th1 immune response.Conclusions:Our study demonstrates that rBmHSP60 has immunogenic properties which effectively enhances the Th1 type immune responses,and can be used as an immunoprophylactic agent against leishmaniasis.Furthermore,in vivo studies are in progress to determine the protective role of rBmHSP60 against Leishmania donovani infection in a mouse model.
