目的:探讨长链非编码RNA TFAP2A-AS1对子宫内膜癌细胞增殖、侵袭和迁移的影响及机制。方法:选择50例子宫内膜癌组织和对应的癌旁组织。选取子宫内膜癌细胞株(RL95-2、HEC-1A、HHUA、HEC-1B及Ishikawa),子宫内膜上皮细胞hEEC。子宫内膜...目的:探讨长链非编码RNA TFAP2A-AS1对子宫内膜癌细胞增殖、侵袭和迁移的影响及机制。方法:选择50例子宫内膜癌组织和对应的癌旁组织。选取子宫内膜癌细胞株(RL95-2、HEC-1A、HHUA、HEC-1B及Ishikawa),子宫内膜上皮细胞hEEC。子宫内膜癌细胞株Ishikawa转染si-TFAP2A-AS1(si-TFAP2A-AS1组)、si-NC(si-NC组)、miR-9-5p mimics(miR-9-5p组)及miR-NC(miR-NC组);RL95-2细胞转染OE-TFAP2A-AS1(TFAP2A-AS1组)、Vector(Vector组)、sh-miR-9-5p(sh-miR-9-5p组)及sh-NC(sh-NC组)。CCK-8检测细胞增殖能力,Transwell检测细胞侵袭、迁移能力,双荧光素酶实验、pull down实验分析TFAP2A-AS1与miR-9-5p的靶向关系;miR-9-5p与ERK的靶向关系,实时荧光定量PCR(RT-qPCR)检测子宫内膜癌组织、癌旁组织、子宫内膜癌细胞及hEEC细胞TFAP2AAS1、miR-9-5p表达水平,Western blot检测细胞外调节蛋白激酶(ERK)、磷酸化ERK(p-ERK)水平。结果:子宫内膜癌组织中TFAP2A-AS1表达水平低于癌旁组织,miR-9-5p表达水平高于癌旁组织(P<0.05)。子宫内膜癌组织TFAP2A-AS1、miR-9-5p表达水平与分化程度、肿瘤直径、TNM分期及淋巴结转移有关。子宫内膜癌组织中TFAP2A-AS1、miR-9-5p表达水平呈负相关(r=-0.782,P=0.002)。与hEEC细胞比较,RL95-2、HEC-1A、HHUA、HEC-1B及Ishikawa细胞TFAP2A-AS1表达水平降低,miR-9-5p表达水平升高(P<0.05)。与si-NC组比较,si-TFAP2A-AS1组细胞48 h、72 h OD值,侵袭、迁移细胞数均升高(P<0.05);与Vector组比较,TFAP2A-AS1组细胞48 h、72 h OD值,侵袭、迁移细胞数均降低(P<0.05)。与miR-NC组比较,miR-9-5p组细胞48 h、72 h OD值,侵袭、迁移细胞数均升高(P<0.05);与sh-NC组比较,sh-miR-9-5p组细胞48 h、72 h OD值,侵袭、迁移细胞数均降低(P<0.05)。TFAP2A-AS1负性调控miR-9-5p、p-ERK表达水平,miR-9-5p可靶向调控ERK蛋白。结论:过表达TFAP2A-AS1通过靶向下调miR-9-5p,抑制ERK通路,最终抑制子宫内膜癌细胞恶性生物学行为。展开更多
BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of gr...BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of great significance to find reliable prog-nostic indicators to guide the treatment and management of patients with gastric cancer.AIM To explore the relationship between serum levels of five biomarkers[carcinoem-bryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4,CA24-2,and ferritin]and prognosis in patients with gastric cancer.METHODS This study included 200 patients with gastric adenocarcinoma,and conducted an in-depth analysis of their baseline characteristics,relationship between tumor markers and staging,and prognosis.The study found that CA19-9 has a signi-ficant correlation with tumor stage,the average levels of CA24-2,CEA,CA72-4 and ferritin were slightly increased disregarding the stage of tumor.Survival analysis showed that increases in CEA,CA19-9,CA24-2,and ferritin were all associated with shortened overall survival of patients.Further multivariate ana-lysis revealed that elevated serum CA72-4 levels were an inde-pendent adverse prognostic factor.RESULTS This study reveals that there is a significant correlation between the expression levels of serum tumor markers CEA,CA19-9,CA72-4,CA24-2 and ferritin in patients with gastric cancer and prognosis,and can be used as important indicators for prognostic evaluation of gastric cancer.In particular,markers that appear abnormally elevated initially may help identify gastric cancer patients with poor prognosis.CONCLUSION Serum CEA and CA19-9 play an important role in the prognosis assessment of gastric cancer,and are effective tools to guide clinical practice and optimize individualized treatment strategies for gastric cancer patients.展开更多
文摘目的:探讨长链非编码RNA TFAP2A-AS1对子宫内膜癌细胞增殖、侵袭和迁移的影响及机制。方法:选择50例子宫内膜癌组织和对应的癌旁组织。选取子宫内膜癌细胞株(RL95-2、HEC-1A、HHUA、HEC-1B及Ishikawa),子宫内膜上皮细胞hEEC。子宫内膜癌细胞株Ishikawa转染si-TFAP2A-AS1(si-TFAP2A-AS1组)、si-NC(si-NC组)、miR-9-5p mimics(miR-9-5p组)及miR-NC(miR-NC组);RL95-2细胞转染OE-TFAP2A-AS1(TFAP2A-AS1组)、Vector(Vector组)、sh-miR-9-5p(sh-miR-9-5p组)及sh-NC(sh-NC组)。CCK-8检测细胞增殖能力,Transwell检测细胞侵袭、迁移能力,双荧光素酶实验、pull down实验分析TFAP2A-AS1与miR-9-5p的靶向关系;miR-9-5p与ERK的靶向关系,实时荧光定量PCR(RT-qPCR)检测子宫内膜癌组织、癌旁组织、子宫内膜癌细胞及hEEC细胞TFAP2AAS1、miR-9-5p表达水平,Western blot检测细胞外调节蛋白激酶(ERK)、磷酸化ERK(p-ERK)水平。结果:子宫内膜癌组织中TFAP2A-AS1表达水平低于癌旁组织,miR-9-5p表达水平高于癌旁组织(P<0.05)。子宫内膜癌组织TFAP2A-AS1、miR-9-5p表达水平与分化程度、肿瘤直径、TNM分期及淋巴结转移有关。子宫内膜癌组织中TFAP2A-AS1、miR-9-5p表达水平呈负相关(r=-0.782,P=0.002)。与hEEC细胞比较,RL95-2、HEC-1A、HHUA、HEC-1B及Ishikawa细胞TFAP2A-AS1表达水平降低,miR-9-5p表达水平升高(P<0.05)。与si-NC组比较,si-TFAP2A-AS1组细胞48 h、72 h OD值,侵袭、迁移细胞数均升高(P<0.05);与Vector组比较,TFAP2A-AS1组细胞48 h、72 h OD值,侵袭、迁移细胞数均降低(P<0.05)。与miR-NC组比较,miR-9-5p组细胞48 h、72 h OD值,侵袭、迁移细胞数均升高(P<0.05);与sh-NC组比较,sh-miR-9-5p组细胞48 h、72 h OD值,侵袭、迁移细胞数均降低(P<0.05)。TFAP2A-AS1负性调控miR-9-5p、p-ERK表达水平,miR-9-5p可靶向调控ERK蛋白。结论:过表达TFAP2A-AS1通过靶向下调miR-9-5p,抑制ERK通路,最终抑制子宫内膜癌细胞恶性生物学行为。
文摘BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of great significance to find reliable prog-nostic indicators to guide the treatment and management of patients with gastric cancer.AIM To explore the relationship between serum levels of five biomarkers[carcinoem-bryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4,CA24-2,and ferritin]and prognosis in patients with gastric cancer.METHODS This study included 200 patients with gastric adenocarcinoma,and conducted an in-depth analysis of their baseline characteristics,relationship between tumor markers and staging,and prognosis.The study found that CA19-9 has a signi-ficant correlation with tumor stage,the average levels of CA24-2,CEA,CA72-4 and ferritin were slightly increased disregarding the stage of tumor.Survival analysis showed that increases in CEA,CA19-9,CA24-2,and ferritin were all associated with shortened overall survival of patients.Further multivariate ana-lysis revealed that elevated serum CA72-4 levels were an inde-pendent adverse prognostic factor.RESULTS This study reveals that there is a significant correlation between the expression levels of serum tumor markers CEA,CA19-9,CA72-4,CA24-2 and ferritin in patients with gastric cancer and prognosis,and can be used as important indicators for prognostic evaluation of gastric cancer.In particular,markers that appear abnormally elevated initially may help identify gastric cancer patients with poor prognosis.CONCLUSION Serum CEA and CA19-9 play an important role in the prognosis assessment of gastric cancer,and are effective tools to guide clinical practice and optimize individualized treatment strategies for gastric cancer patients.
