To the editor,Sir,the emerging influenza infection is the present global concern.There are many new emerging influenza infections at present and those infections are usually problematic.Of several new infections,the m...To the editor,Sir,the emerging influenza infection is the present global concern.There are many new emerging influenza infections at present and those infections are usually problematic.Of several new infections,the most recent emerging ones include to H6N1,H5N6 and H10N8 influenza infections.To learn the natural history of those three new emerging infections is very interesting and can give the data for further referencing.Here,the authors展开更多
In June 2013,the fi rst human H6N1 infl uenza virus infec-tion was confirmed in Taiwan.However,the origin and molecular characterization of this virus,A/Taiwan/2/2013(H6N1),have not been well studied thus far.In the p...In June 2013,the fi rst human H6N1 infl uenza virus infec-tion was confirmed in Taiwan.However,the origin and molecular characterization of this virus,A/Taiwan/2/2013(H6N1),have not been well studied thus far.In the present report,we performed phylogenetic and coalescent analy-ses of this virus and compared its molecular profi le/char-acteristics with other closely related strains.Molecular characterization of H6N1 revealed that it is a typical avian infl uenza virus of low pathogenicity,which might not rep-licate and propagate well in the upper airway in mammals.Phylogenetic analysis revealed that the virus clusters with A/chicken/Taiwan/A2837/2013(H6N1)in seven genes,except PB1.For the PB1 gene,A/Taiwan/2/2013 was clus-tered with a different H6N1 lineage from A/chicken/Taiwan/A2837/2013.Although a previous study demonstrated that the PB2,PA,and M genes of A/Taiwan/2/2013 might be derived from the H5N2 viruses,coalescent analyses revealed that these H5N2 viruses were derived from more recent strains than that of the ancestor of A/Taiwan/2/2013.Therefore,we propose that A/Taiwan/2/2013 is a reassor-tant from different H6N1 lineages circulating in chickens in Taiwan.Furthermore,compared to avian isolates,a sin-gle P186L(H3 numbering)substitution in the hemaggluti-nin H6 of the human isolate might increase the mammali-an receptor binding and,hence,this strain’s pathogenicity in humans.Overall,human infection with this virus seems an accidental event and is unlikely to cause an infl uenza pandemic.However,its co-circulation and potential reas-sortment with other infl uenza subtypes are still worthy of attention.展开更多
根据基因库中H6亚型禽流感病毒（AIV） HA基因、N1亚型AIV NA基因和所有亚型AIV M基因序列,分别设计筛选出3对特异性引物,优化反应条件,建立了H6N1亚型AIV GeXP检测方法。该法对H6亚型AIV的检测出现194 bp （H6）、164 bp （AIV通用检测...根据基因库中H6亚型禽流感病毒（AIV） HA基因、N1亚型AIV NA基因和所有亚型AIV M基因序列,分别设计筛选出3对特异性引物,优化反应条件,建立了H6N1亚型AIV GeXP检测方法。该法对H6亚型AIV的检测出现194 bp （H6）、164 bp （AIV通用检测） 2个目的峰,对N1亚型AIV的检测出现249 bp （N1）、164 bp 2个目的峰,对H6N1亚型AIV的检测出现194 bp、249 bp和164 bp 3个目的峰,对其他亚型AIV的检测只出现164 bp通用检测目的峰,对常见禽病病原体均未出现任何检测峰;该法对H6亚型和N1亚型AIV检测下限为102拷贝/μL。本研究建立的H6亚型和N1亚型AIV高通量GeXP检测方法特异性强、灵敏度高,可同时快速鉴别检测H6亚型、N1亚型和所有亚型AIV,为H6N1亚型AIV的检测提供了一种简便、快速和有效的方法。展开更多
文摘To the editor,Sir,the emerging influenza infection is the present global concern.There are many new emerging influenza infections at present and those infections are usually problematic.Of several new infections,the most recent emerging ones include to H6N1,H5N6 and H10N8 influenza infections.To learn the natural history of those three new emerging infections is very interesting and can give the data for further referencing.Here,the authors
基金the National Basic Research Pro-gram(973 Program)(Nos.2010CB530303,2011CB504703,and 2012CB955501an intramural special grant for influenza virus research from the Chinese Academy of Sciences(KSZD-EW-Z-002)the Doctoral Starting up Foundation of Taishan Medical College.GFG is a leading principal investigator of the Innova-tive Research Group of the National Natural Science Foundation of China(Grant No.81021003)。
文摘In June 2013,the fi rst human H6N1 infl uenza virus infec-tion was confirmed in Taiwan.However,the origin and molecular characterization of this virus,A/Taiwan/2/2013(H6N1),have not been well studied thus far.In the present report,we performed phylogenetic and coalescent analy-ses of this virus and compared its molecular profi le/char-acteristics with other closely related strains.Molecular characterization of H6N1 revealed that it is a typical avian infl uenza virus of low pathogenicity,which might not rep-licate and propagate well in the upper airway in mammals.Phylogenetic analysis revealed that the virus clusters with A/chicken/Taiwan/A2837/2013(H6N1)in seven genes,except PB1.For the PB1 gene,A/Taiwan/2/2013 was clus-tered with a different H6N1 lineage from A/chicken/Taiwan/A2837/2013.Although a previous study demonstrated that the PB2,PA,and M genes of A/Taiwan/2/2013 might be derived from the H5N2 viruses,coalescent analyses revealed that these H5N2 viruses were derived from more recent strains than that of the ancestor of A/Taiwan/2/2013.Therefore,we propose that A/Taiwan/2/2013 is a reassor-tant from different H6N1 lineages circulating in chickens in Taiwan.Furthermore,compared to avian isolates,a sin-gle P186L(H3 numbering)substitution in the hemaggluti-nin H6 of the human isolate might increase the mammali-an receptor binding and,hence,this strain’s pathogenicity in humans.Overall,human infection with this virus seems an accidental event and is unlikely to cause an infl uenza pandemic.However,its co-circulation and potential reas-sortment with other infl uenza subtypes are still worthy of attention.
文摘根据基因库中H6亚型禽流感病毒（AIV） HA基因、N1亚型AIV NA基因和所有亚型AIV M基因序列,分别设计筛选出3对特异性引物,优化反应条件,建立了H6N1亚型AIV GeXP检测方法。该法对H6亚型AIV的检测出现194 bp （H6）、164 bp （AIV通用检测） 2个目的峰,对N1亚型AIV的检测出现249 bp （N1）、164 bp 2个目的峰,对H6N1亚型AIV的检测出现194 bp、249 bp和164 bp 3个目的峰,对其他亚型AIV的检测只出现164 bp通用检测目的峰,对常见禽病病原体均未出现任何检测峰;该法对H6亚型和N1亚型AIV检测下限为102拷贝/μL。本研究建立的H6亚型和N1亚型AIV高通量GeXP检测方法特异性强、灵敏度高,可同时快速鉴别检测H6亚型、N1亚型和所有亚型AIV,为H6N1亚型AIV的检测提供了一种简便、快速和有效的方法。
