The H6P2W18O62/TiO2composite catalyst was prepared by the combination of nonionic surfactant C18H37(OCH2CH2)10OH(Brij-76)as the template and the sol-gel method.As-synthesized composite was characterized by FT-TR,SEM,N...The H6P2W18O62/TiO2composite catalyst was prepared by the combination of nonionic surfactant C18H37(OCH2CH2)10OH(Brij-76)as the template and the sol-gel method.As-synthesized composite was characterized by FT-TR,SEM,N2 absorption-desorption and NH3-TPD.The results showed that the composite H6P2W18O62/TiO2 was mesoporous material(ca.3.3 nm),and large surface area(99.78 m2/g).Additionally,the aggregation of TiO2 particles was effectively inhibited,and the surface acidity was increased substantially.The photocatalytic elimination of monochlorobenzene was used as model reaction to evaluate the photocatalytic activity of the composite catalyst under visible light separately.Photocatalytic experimental results showed that the composite H6P2W18O62/TiO2 can effectively degradate monochlorobenzene.展开更多
目的:探讨6p21.1 rs2494938和7p15.3 rs2285947基因多态性与宫颈癌发生风险的关联性。方法:以571例宫颈癌患者和657例非宫颈癌患者(对照组)为研究对象,用Taq Man MGB(minor grove binder)探针对6p21.1 rs2494938多态位点和7p15.3 rs2285...目的:探讨6p21.1 rs2494938和7p15.3 rs2285947基因多态性与宫颈癌发生风险的关联性。方法:以571例宫颈癌患者和657例非宫颈癌患者(对照组)为研究对象,用Taq Man MGB(minor grove binder)探针对6p21.1 rs2494938多态位点和7p15.3 rs2285947多态位点进行基因分型,分析不同基因型与宫颈癌发生风险的关联性。采用非条件Logistic回归分析统计该多态位点与宫颈癌遗传易感的关联性,计算相对危险度的比值比(OR)及95%置信区间(CI)。结果:rs2494938多态位点突变型GA和AA基因型频率在病例组和对照组的分布无显著差异(P=0.848)。rs2285947多态位点突变型GA和AA基因型频率在病例组和对照组的分布有显著差异(P=0.028);合并突变基因型(GA+AA)与野生型GG相比宫颈癌发生风险显著下降(OR=0.77,95%CI:0.62~0.97,P=0.025)。结论:rs2494938多态性与宫颈癌发生风险无显著关联,rs2285947多态性与宫颈癌发生风险有显著关联。展开更多
文摘目的从轮状病毒阳性的牛粪便标本中,分离出一株G6P[1]型牛轮状病毒(Bovine Rotavirus,BRV),对其进行培养和鉴定。方法用PBS溶液重悬粪便标本并离心,将其上清过滤除菌和胰酶处理后,利用MA104细胞进行分离培养;通过逆转录-聚合酶链式反应(Reverse Transcription-polymerase Chain Reaction,RT-PCR)对样本VP4和VP7基因进行扩增和测序,与GenBank上的参考序列进行同源性分析,构建进化树,分析确定其G/P基因分型。通过聚丙烯酰胺凝胶电泳法(Polyacrylamine Gel Electrophoresis,PAGE)、噬斑实验和电镜(Transmission Electron Microscope,TEM)等技术对分离到的病毒进行鉴定和纯化。绘制病毒生长动力学曲线。结果分离培养了1株BRV毒株,将其命名BLL。VP7和VP4基因测序结果显示此毒株为G6P[1]型轮状病毒。PAGE胶结果显示分离株电泳型为长型,条带呈现A组轮状病毒排列电泳图谱;通过噬斑实验将毒株进行了纯化。电镜检测到典型的轮状病毒颗粒。病毒生长动力学曲线可发现病毒在感染后6 h已经开始复制。结论本研究成功分离到G6P[1]型牛型轮状病毒,为研究G6P[1]型轮状病毒的病原学特征提供实验基础和技术参考。
文摘The H6P2W18O62/TiO2composite catalyst was prepared by the combination of nonionic surfactant C18H37(OCH2CH2)10OH(Brij-76)as the template and the sol-gel method.As-synthesized composite was characterized by FT-TR,SEM,N2 absorption-desorption and NH3-TPD.The results showed that the composite H6P2W18O62/TiO2 was mesoporous material(ca.3.3 nm),and large surface area(99.78 m2/g).Additionally,the aggregation of TiO2 particles was effectively inhibited,and the surface acidity was increased substantially.The photocatalytic elimination of monochlorobenzene was used as model reaction to evaluate the photocatalytic activity of the composite catalyst under visible light separately.Photocatalytic experimental results showed that the composite H6P2W18O62/TiO2 can effectively degradate monochlorobenzene.
基金2013年国家级大学生创新创业训练计划项目(201313256001)湖北师范学院硕士研究生创新科研基金项目(1051320130216)+1 种基金National Undergraduate Training Programs for Innovation and Entrepreneurship(201313256001)Postgraduate Innovation Scientific Research Foundation of Hubei Normal University(1051320130216)
文摘目的:探讨6p21.1 rs2494938和7p15.3 rs2285947基因多态性与宫颈癌发生风险的关联性。方法:以571例宫颈癌患者和657例非宫颈癌患者(对照组)为研究对象,用Taq Man MGB(minor grove binder)探针对6p21.1 rs2494938多态位点和7p15.3 rs2285947多态位点进行基因分型,分析不同基因型与宫颈癌发生风险的关联性。采用非条件Logistic回归分析统计该多态位点与宫颈癌遗传易感的关联性,计算相对危险度的比值比(OR)及95%置信区间(CI)。结果:rs2494938多态位点突变型GA和AA基因型频率在病例组和对照组的分布无显著差异(P=0.848)。rs2285947多态位点突变型GA和AA基因型频率在病例组和对照组的分布有显著差异(P=0.028);合并突变基因型(GA+AA)与野生型GG相比宫颈癌发生风险显著下降(OR=0.77,95%CI:0.62~0.97,P=0.025)。结论:rs2494938多态性与宫颈癌发生风险无显著关联,rs2285947多态性与宫颈癌发生风险有显著关联。
