To analyze the resistance of Lianjing 7 against rice black-streaked dwarf disease and find the molecular markers closely linked with the resistance gene, an F2：3 population derived from an RBSDD-resistant cultivar Li...To analyze the resistance of Lianjing 7 against rice black-streaked dwarf disease and find the molecular markers closely linked with the resistance gene, an F2：3 population derived from an RBSDD-resistant cultivar Lianjing 7 and an RBSDD-susceptible cultivar Peiai 64, was evaluated using natural infection methods and artificial inoculation by scoring the disease severity rating. The results showed Lianjing 7 was resistant to RBSDD, with the incidence between 5.5% and 12.25% in all tests. For the parents of Lianjing 7, only Zhongjingchuan-2 was resistant to RBSDD. Besides this, Lianjing 7 assumed an antixenosis and antibiosis score similar with susceptible variety Wuyujing 3, which indicates that so RBSDD-resistance of Lianjing 7 is mainly caused by the resistance specifically to RBSDV. In the F2：3 population of Lianjing 7/Peiai 64, the disease severity rates showed a continuous distribution with transgressive segregation, it indicated the resistance of Lianjing 7 was controlled by multi-genes. The marker RM287 was determined closely linked with the resistance locus via BSA method. To conclude, Lianjing 7 has a high resistance to RBSDD, which is conferred with by Zhongjingchuan-2, and the marker RM287 should be useful for breeding novel RBSDD-resistant cultivars via marker-assisted selection （MAS）.展开更多
基金Supported by Natural Science Foundation of Jiangsu Province(SBK201122073)Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province(CX(12)5112,1003-2)+1 种基金Key Science and Technology Program of Lianyungang City(Agriculture,CN1201)National Rice Industry System Development Program(CARS-01-02A)~~
文摘To analyze the resistance of Lianjing 7 against rice black-streaked dwarf disease and find the molecular markers closely linked with the resistance gene, an F2：3 population derived from an RBSDD-resistant cultivar Lianjing 7 and an RBSDD-susceptible cultivar Peiai 64, was evaluated using natural infection methods and artificial inoculation by scoring the disease severity rating. The results showed Lianjing 7 was resistant to RBSDD, with the incidence between 5.5% and 12.25% in all tests. For the parents of Lianjing 7, only Zhongjingchuan-2 was resistant to RBSDD. Besides this, Lianjing 7 assumed an antixenosis and antibiosis score similar with susceptible variety Wuyujing 3, which indicates that so RBSDD-resistance of Lianjing 7 is mainly caused by the resistance specifically to RBSDV. In the F2：3 population of Lianjing 7/Peiai 64, the disease severity rates showed a continuous distribution with transgressive segregation, it indicated the resistance of Lianjing 7 was controlled by multi-genes. The marker RM287 was determined closely linked with the resistance locus via BSA method. To conclude, Lianjing 7 has a high resistance to RBSDD, which is conferred with by Zhongjingchuan-2, and the marker RM287 should be useful for breeding novel RBSDD-resistant cultivars via marker-assisted selection （MAS）.
