Objective: Urine 8-hydroxy-deoxyguanosine (U8-OHdG) was a marker of oxidative stress-induced DNA damage;it was increased by psychological stress. Since the menstrual cycle may confound or modify the association betwee...Objective: Urine 8-hydroxy-deoxyguanosine (U8-OHdG) was a marker of oxidative stress-induced DNA damage;it was increased by psychological stress. Since the menstrual cycle may confound or modify the association between psychological stress and U8-OHdG. The aim of this study was to verify the effect of psychological stress from a national license examination on levels of U8-OHdG, which is a biomarker of oxidative stress. And the effects of women’s menstrual cycles, which should be considered in mental and physical assessments, on U8-OHdG, were evaluated. Methods: The subjects were 18 female university students at a medical university in whom Self-rating Depression Scale (SDS) scores, State-Trait Anxiety Inventory (STAI) scores, and U8-OHdG levels were measured in 3 phases of the menstrual cycle, the follicular phase, ovulatory phase, and luteal phase. The mean values were taken for the group during a time of classroom learning. The same measurements were also made one week before and the day after a national license examination and the measurements were compared among the three periods. Results and Conclusion: State anxiety and U8-OHdG levels were significantly higher in those with a week before the national license examination than in those with classroom lecture (State anxiety: p = 0.002, U8-OHdG levels: p = 0.007). The menstrual cycle did not show a significant correlation with U8-OHdG levels. This study demonstrated that levels of the oxidative stress biomarker U8-OHdG are not affected by changes in the menstrual cycle. It also showed that U8-OHdG levels increased with the psychological stress of a national license examination.展开更多
目的:探讨尿液8-OHdG、血清IL-6与糖尿病肾脏病相关性。方法:收集2017年09月—2019年05月就诊于东直门医院糖尿病肾脏病患者123例,收集受试者人口学信息、一般临床资料,进行肾功能、24 h尿蛋白定量等相关检查。依据DKD诊断、CKD分期、Mo...目的:探讨尿液8-OHdG、血清IL-6与糖尿病肾脏病相关性。方法:收集2017年09月—2019年05月就诊于东直门医院糖尿病肾脏病患者123例,收集受试者人口学信息、一般临床资料,进行肾功能、24 h尿蛋白定量等相关检查。依据DKD诊断、CKD分期、Mongensen分期等标准,将糖尿病肾脏病患者分为微量白蛋白尿期、大量蛋白尿期、肾衰竭早期,留取标本,检测尿液8-OHdG、血清IL-6水平。结果:微量白蛋白尿期、大量蛋白尿期、肾衰竭早期患者尿液8-OHdG(P=0.000)、血清IL-6(P=0.000)水平依次升高,差异有统计学意义。采用Spearman相关分析,尿液8-OHdG与24 h UTP呈高度正相关性(r=0.748,P=0.000),与eGFR呈中度负相关性(r=-0.502,P=0.000);血清IL-6水平与24 h UTP呈低度正相关性(r=0.397,P=0.000),与eGFR(CKD-EPI公式)呈低度负相关性(r=-0.330,P=0.000)。结论:尿液8-OHdG与DKD中高度相关,血清IL-6与DKD低度相关性,尿液8-OHdG、血清IL-6代表的氧化应激、炎症为2型糖尿病肾脏疾病的影响因素,尿液8-OHdG有可能成为反应糖尿病肾脏病病情进展及疗效评价的生物学标志物,且有望成为诊疗糖尿病肾脏病潜在的靶标。展开更多
文摘Objective: Urine 8-hydroxy-deoxyguanosine (U8-OHdG) was a marker of oxidative stress-induced DNA damage;it was increased by psychological stress. Since the menstrual cycle may confound or modify the association between psychological stress and U8-OHdG. The aim of this study was to verify the effect of psychological stress from a national license examination on levels of U8-OHdG, which is a biomarker of oxidative stress. And the effects of women’s menstrual cycles, which should be considered in mental and physical assessments, on U8-OHdG, were evaluated. Methods: The subjects were 18 female university students at a medical university in whom Self-rating Depression Scale (SDS) scores, State-Trait Anxiety Inventory (STAI) scores, and U8-OHdG levels were measured in 3 phases of the menstrual cycle, the follicular phase, ovulatory phase, and luteal phase. The mean values were taken for the group during a time of classroom learning. The same measurements were also made one week before and the day after a national license examination and the measurements were compared among the three periods. Results and Conclusion: State anxiety and U8-OHdG levels were significantly higher in those with a week before the national license examination than in those with classroom lecture (State anxiety: p = 0.002, U8-OHdG levels: p = 0.007). The menstrual cycle did not show a significant correlation with U8-OHdG levels. This study demonstrated that levels of the oxidative stress biomarker U8-OHdG are not affected by changes in the menstrual cycle. It also showed that U8-OHdG levels increased with the psychological stress of a national license examination.
文摘目的:探讨尿液8-OHdG、血清IL-6与糖尿病肾脏病相关性。方法:收集2017年09月—2019年05月就诊于东直门医院糖尿病肾脏病患者123例,收集受试者人口学信息、一般临床资料,进行肾功能、24 h尿蛋白定量等相关检查。依据DKD诊断、CKD分期、Mongensen分期等标准,将糖尿病肾脏病患者分为微量白蛋白尿期、大量蛋白尿期、肾衰竭早期,留取标本,检测尿液8-OHdG、血清IL-6水平。结果:微量白蛋白尿期、大量蛋白尿期、肾衰竭早期患者尿液8-OHdG(P=0.000)、血清IL-6(P=0.000)水平依次升高,差异有统计学意义。采用Spearman相关分析,尿液8-OHdG与24 h UTP呈高度正相关性(r=0.748,P=0.000),与eGFR呈中度负相关性(r=-0.502,P=0.000);血清IL-6水平与24 h UTP呈低度正相关性(r=0.397,P=0.000),与eGFR(CKD-EPI公式)呈低度负相关性(r=-0.330,P=0.000)。结论:尿液8-OHdG与DKD中高度相关,血清IL-6与DKD低度相关性,尿液8-OHdG、血清IL-6代表的氧化应激、炎症为2型糖尿病肾脏疾病的影响因素,尿液8-OHdG有可能成为反应糖尿病肾脏病病情进展及疗效评价的生物学标志物,且有望成为诊疗糖尿病肾脏病潜在的靶标。
