目的探讨尿液中核酸氧化代谢产物8-oxo-Gsn和8-oxo-d Gsn对HBV感染引起肝损伤程度的评估价值。方法收集笔者医院94例慢性乙型肝炎(以下简称慢乙肝)患者行超声引导下肝穿刺活检前的尿液,95例健康者以及25例乙肝表面携带者的随机尿液,采...目的探讨尿液中核酸氧化代谢产物8-oxo-Gsn和8-oxo-d Gsn对HBV感染引起肝损伤程度的评估价值。方法收集笔者医院94例慢性乙型肝炎(以下简称慢乙肝)患者行超声引导下肝穿刺活检前的尿液,95例健康者以及25例乙肝表面携带者的随机尿液,采用同位素稀释高效液相-串联质谱法(ID-LC-MS/MS)分析尿液中DNA氧化标志物8-oxod Gsn和RNA氧化标志物8-oxo-Gsn,并结合病理结果和生化指标(ALT和AST),分析各指标之间的相关性,通过ROC曲线评估二者对肝损伤程度的预测价值,并分析8-oxo-Gsn和8-oxo-d Gsn在不同炎症活动度和不同纤维化程度患者中的水平差异。结果实验组中,88.5%(46/52)的男性8-oxo-Gsn高于正常对照组,85.7%(36/42)的女性8-oxo-Gsn高于正常对照组;肝脏炎症程度高的患者(G_3~G_4)尿液中RNA氧化标志物8-oxo-Gsn显著高于炎症程度低的患者(G_0~G_2)(4.28 vs 3.26,χ~2=11.117,P=0.009),纤维化程度高的患者8-oxo-Gsn水平也高于纤维化程度低的患者(3.66 vs 3.29),但未达到显著性水平(χ~2=3.626,P=0.323)。尿液中RNA氧化标志物8-oxo-Gsn对肝脏炎症程度有较好的预测价值(敏感度为82.4%,特异性为75%,AUC=0.813),对纤维化程度有较好的预测价值(敏感度为83.3%,特异性为77.1%,AUC=0.824)。结论尿液中RNA氧化标志物8-oxo-Gsn可能是HBV感染引起的肝损伤严重程度的评估指标。展开更多
Heat shock protein family B(small)member 8(HSPB8)is a 22 kDa ubiquitously expressed protein belonging to the family of small heat shock proteins.HSPB8 is involved in various cellular mechanisms mainly related to prote...Heat shock protein family B(small)member 8(HSPB8)is a 22 kDa ubiquitously expressed protein belonging to the family of small heat shock proteins.HSPB8 is involved in various cellular mechanisms mainly related to proteotoxic stress response and in other processes such as inflammation,cell division,and migration.HSPB8 binds misfolded clients to prevent their aggregation by assisting protein refolding or degradation through chaperone-assisted selective autophagy.In line with this function,the pro-degradative activity of HSPB8 has been found protective in several neurodegenerative and neuromuscular diseases characterized by protein misfolding and aggregation.In cancer,HSPB8 has a dual role being capable of exerting either a pro-or an anti-tumoral activity depending on the pathways and factors expressed by the model of cancer under investigation.Moreover,HSPB8 exerts a protective function in different diseases by modulating the inflammatory response,which characterizes not only neurodegenerative diseases,but also other chronic or acute conditions affecting the nervous system,such as multiple sclerosis and intracerebellar hemorrhage.Of note,HSPB8 modulation may represent a therapeutic approach in other neurological conditions that develop as a secondary consequence of other diseases.This is the case of cognitive impairment related to diabetes mellitus,in which HSPB8 exerts a protective activity by assuring mitochondrial homeostasis.This review aims to summarize the diverse and multiple functions of HSPB8 in different pathological conditions,focusing on the beneficial effects of its modulation.Drug-based and alternative therapeutic approaches targeting HSPB8 and its regulated pathways will be discussed,emphasizing how new strategies for cell and tissue-specific delivery represent an avenue to advance in disease treatments.展开更多
文摘目的探讨尿液中核酸氧化代谢产物8-oxo-Gsn和8-oxo-d Gsn对HBV感染引起肝损伤程度的评估价值。方法收集笔者医院94例慢性乙型肝炎(以下简称慢乙肝)患者行超声引导下肝穿刺活检前的尿液,95例健康者以及25例乙肝表面携带者的随机尿液,采用同位素稀释高效液相-串联质谱法(ID-LC-MS/MS)分析尿液中DNA氧化标志物8-oxod Gsn和RNA氧化标志物8-oxo-Gsn,并结合病理结果和生化指标(ALT和AST),分析各指标之间的相关性,通过ROC曲线评估二者对肝损伤程度的预测价值,并分析8-oxo-Gsn和8-oxo-d Gsn在不同炎症活动度和不同纤维化程度患者中的水平差异。结果实验组中,88.5%(46/52)的男性8-oxo-Gsn高于正常对照组,85.7%(36/42)的女性8-oxo-Gsn高于正常对照组;肝脏炎症程度高的患者(G_3~G_4)尿液中RNA氧化标志物8-oxo-Gsn显著高于炎症程度低的患者(G_0~G_2)(4.28 vs 3.26,χ~2=11.117,P=0.009),纤维化程度高的患者8-oxo-Gsn水平也高于纤维化程度低的患者(3.66 vs 3.29),但未达到显著性水平(χ~2=3.626,P=0.323)。尿液中RNA氧化标志物8-oxo-Gsn对肝脏炎症程度有较好的预测价值(敏感度为82.4%,特异性为75%,AUC=0.813),对纤维化程度有较好的预测价值(敏感度为83.3%,特异性为77.1%,AUC=0.824)。结论尿液中RNA氧化标志物8-oxo-Gsn可能是HBV感染引起的肝损伤严重程度的评估指标。
基金supported by:Fondazione Telethon-Italy(No.GGP19128 to AP)Fondazione Cariplo-Italy(No.2021-1544 to RC)+14 种基金Fondazione Italiana di Ricerca per la Sclerosi Laterale Amiotrofica(AriSLA)-Italy(No.MLOpathy to APTarget-RAN to AP)Association Française contre les Myopathies-France(AFM Telethon No.23236 to AP)Kennedy’s Disease Association-USA(2018 grant to RC2020 grant to MG)Ministero dell’Universitàe della Ricerca(MIUR)-Italy(PRIN-Progetti di ricerca di interesse nazionale(No.2017F2A2C5 to APNo.2022EFLFL8 to APNo.2020PBS5MJ to VCNo.2022KSJZF5 to VC)PRIN-Progetti di ricerca di interesse nazionale-bando 2022,PNRR finanziato dall’Unione europea-Next Generation EU,componente M4C2,investimento 1.1(No.P2022B5J32 to RC and No.P20225R4Y5 to VC)CN3:RNA-Codice Proposta:CN_00000041Tematica Sviluppo di terapia genica e farmaci con tecnologia a RNA(Centro Nazionale di Ricerca-CN3 National Center for Gene Therapy and Drugs based on RNA Technology to AP)Progetto Dipartimenti di Eccellenza(to DiSFeB)Ministero della Salute,Agenzia Italiana del Farmaco(AIFA)-Italy(Co_ALS to AP)Universitàdegli Studi di Milano(piano di sviluppo della ricerca(PSR)UNIMI-linea B(to RC and BT).
文摘Heat shock protein family B(small)member 8(HSPB8)is a 22 kDa ubiquitously expressed protein belonging to the family of small heat shock proteins.HSPB8 is involved in various cellular mechanisms mainly related to proteotoxic stress response and in other processes such as inflammation,cell division,and migration.HSPB8 binds misfolded clients to prevent their aggregation by assisting protein refolding or degradation through chaperone-assisted selective autophagy.In line with this function,the pro-degradative activity of HSPB8 has been found protective in several neurodegenerative and neuromuscular diseases characterized by protein misfolding and aggregation.In cancer,HSPB8 has a dual role being capable of exerting either a pro-or an anti-tumoral activity depending on the pathways and factors expressed by the model of cancer under investigation.Moreover,HSPB8 exerts a protective function in different diseases by modulating the inflammatory response,which characterizes not only neurodegenerative diseases,but also other chronic or acute conditions affecting the nervous system,such as multiple sclerosis and intracerebellar hemorrhage.Of note,HSPB8 modulation may represent a therapeutic approach in other neurological conditions that develop as a secondary consequence of other diseases.This is the case of cognitive impairment related to diabetes mellitus,in which HSPB8 exerts a protective activity by assuring mitochondrial homeostasis.This review aims to summarize the diverse and multiple functions of HSPB8 in different pathological conditions,focusing on the beneficial effects of its modulation.Drug-based and alternative therapeutic approaches targeting HSPB8 and its regulated pathways will be discussed,emphasizing how new strategies for cell and tissue-specific delivery represent an avenue to advance in disease treatments.
