Objective： To observe the lethal effect of T cells activated by dendritic cells （DCs） with MUC1 gene transfection （MUC1-DCs） on BIU-87 cells in vitro, and to evaluate the possibility of dendritic cells transfecte...Objective： To observe the lethal effect of T cells activated by dendritic cells （DCs） with MUC1 gene transfection （MUC1-DCs） on BIU-87 cells in vitro, and to evaluate the possibility of dendritic cells transfected by MUC1 gene as a vaccine for bladder cancer immunotherapy. Methods： MUC1 was successfully transfected into cultured human blood-derived dendritic cell with lipofectin. The transfection efficiency and immunocompetence were detected by flow cytometry and MTT colourmetry. T lymphocytes activated by MUC1-DCs were used to kill BIU-87 cell lines and normal bladder epithelium in vitro and the killing rate was evaluated by MTT colourmetry. Results： There was significant lethal effect on the BIU-87 cells and normal bladder epithelium with T cells activated by MUCl-transfected DCs, but the lethal effect on the BIU-87 cells significantly exceeded that on normal bladder epithelium （P〈0.05）. The lethal effect on BIU-87 cells with T cells activated by MUCl-transfected DCs significantly exceeded that with T cells activated by no-transfection DCs （P〈0.01）. Conclusion： T cells activated by MUC1-DCs could induce killing action to BIU-87 cell lines. MUC1 gene could be used as a target for bladder cancer immunotherapy.展开更多
基金This project was supported by a grant from National Natural Sciences Foundation of China (No. 30271300)
文摘Objective： To observe the lethal effect of T cells activated by dendritic cells （DCs） with MUC1 gene transfection （MUC1-DCs） on BIU-87 cells in vitro, and to evaluate the possibility of dendritic cells transfected by MUC1 gene as a vaccine for bladder cancer immunotherapy. Methods： MUC1 was successfully transfected into cultured human blood-derived dendritic cell with lipofectin. The transfection efficiency and immunocompetence were detected by flow cytometry and MTT colourmetry. T lymphocytes activated by MUC1-DCs were used to kill BIU-87 cell lines and normal bladder epithelium in vitro and the killing rate was evaluated by MTT colourmetry. Results： There was significant lethal effect on the BIU-87 cells and normal bladder epithelium with T cells activated by MUCl-transfected DCs, but the lethal effect on the BIU-87 cells significantly exceeded that on normal bladder epithelium （P〈0.05）. The lethal effect on BIU-87 cells with T cells activated by MUCl-transfected DCs significantly exceeded that with T cells activated by no-transfection DCs （P〈0.01）. Conclusion： T cells activated by MUC1-DCs could induce killing action to BIU-87 cell lines. MUC1 gene could be used as a target for bladder cancer immunotherapy.
