目的:研究广西不同地区壮族的mtDNA Region V区的遗传多态性及其遗传结构的相关性。方法:采用PCR-PAGE和直接测序广西地区壮族样本线粒体DNA Region V区进行序列分析。结果:在广西壮族样本中只发现标准型和短型两种多态。广西壮族的9-b...目的:研究广西不同地区壮族的mtDNA Region V区的遗传多态性及其遗传结构的相关性。方法:采用PCR-PAGE和直接测序广西地区壮族样本线粒体DNA Region V区进行序列分析。结果:在广西壮族样本中只发现标准型和短型两种多态。广西壮族的9-bp缺失频率平均为18.94%;其中广西南宁、柳州、百色和河池等地区的壮族9-bp缺失频率分别为22.22%、14.58%、12.50%和22.45%(P>0.05)。结论:来自广西不同地区的壮族人群的9-bp缺失频率虽有所不同,但没有明显差异。展开更多
旨在研究中国广东省部分地区汉族人群线粒体DNA Region Ⅴ 9bp序列缺失情况。采用PCR-PAGE和直接测序法对3个群体144份样本mtDNA Region Ⅴ进行序列分析。结果只检测到标准型和短型(即9bp缺失)两种多态。广东汉族人群的平均缺失频率为21...旨在研究中国广东省部分地区汉族人群线粒体DNA Region Ⅴ 9bp序列缺失情况。采用PCR-PAGE和直接测序法对3个群体144份样本mtDNA Region Ⅴ进行序列分析。结果只检测到标准型和短型(即9bp缺失)两种多态。广东汉族人群的平均缺失频率为21.5%,广州、东莞和湛江汉族人群的缺失频率依次为20.8%、19.2%和25.0%。由此得出,广东汉族人群mtDNA9bp缺失频率较高,与其它地区汉族群体存在一定的差异。展开更多
内脂素(Visfatin)是脂肪细胞因子家族的新成员,主要由内脏脂肪组织产生.研究表明内脂素具有类胰岛素样作用.在检测固始鸡-安卡鸡资源群体3代(亲本,F1,F2)964只鸡Visfatin基因9bp插入/缺失(9 bp 'TAACCTGTG' insertion-deletion...内脂素(Visfatin)是脂肪细胞因子家族的新成员,主要由内脏脂肪组织产生.研究表明内脂素具有类胰岛素样作用.在检测固始鸡-安卡鸡资源群体3代(亲本,F1,F2)964只鸡Visfatin基因9bp插入/缺失(9 bp 'TAACCTGTG' insertion-deletion)多态的过程中,发现其杂合子的变性和非变性聚丙烯酰胺胶上除2条同源双链DNA(282bp和273bp)外有2条未知条带(命名为A和B).A,B条带经回收、二次PCR、再次聚丙烯酰胺凝胶电泳及DNA测序表明:Visfatin基因第10内含子中9bp insertion-deletion突变杂合子的PCR产物中,本身包含2种同源双链DNA片段和2种异源双链DNA片段,不需要经过额外的变性、退火处理,其PCR产物可以直接进行突变检测,在229个杂合突变中异源双链DNA的检出率为100%.因此,通过异源双链DNA这一标示物作为基因分型时的依照或者参考,建立适当的异源双链DNA分析法可进行基因中几个核苷酸插入/缺失多态的检测.展开更多
根据中国西北干旱区巴里坤湖BLK-1剖面沉积物的地化元素分析,采用SPSS因子分析法提取了对沉积环境变化敏感的地化元素和氧化物组分.在R、Q型因子分析的基础上,结合沉积物的硅铁铝率(SiO2/(Al2O3+Fe2O3))、淋失系数(SiO2/(MgO+K2O))、CaO...根据中国西北干旱区巴里坤湖BLK-1剖面沉积物的地化元素分析,采用SPSS因子分析法提取了对沉积环境变化敏感的地化元素和氧化物组分.在R、Q型因子分析的基础上,结合沉积物的硅铁铝率(SiO2/(Al2O3+Fe2O3))、淋失系数(SiO2/(MgO+K2O))、CaO/MgO以及腐殖化度等指标,提取出古气候环境信息.初步研究结果表明:近9.0 cal ka BP以来,巴里坤湖地区气候环境仍以干旱化为主,全新世期间出现过多次不同程度的干湿变化,经历了5个气候阶段:9.0—7.5 cal ka BP期间,气候干旱;7.5—5.8 cal ka BP期间,气候温暖湿润,为研究区全新世最佳适宜期;5.8—3.0 cal ka BP期间,气候干旱;3.0—1.0 cal ka BP期间,气候湿润;1.0—0 cal ka BP期间,气候干旱.展开更多
目的研究中国河南地区汉族人群线粒体DNA Region V区的遗传多态性。方法采用聚合酶链反应-聚丙烯酰胺凝胶电泳(PCR-PAGE)法对277份河南汉族样本的线粒体DNA RegionⅤ进行多态性分析,并随机选取部分DNA扩增产物进行测序予以确认。结果在...目的研究中国河南地区汉族人群线粒体DNA Region V区的遗传多态性。方法采用聚合酶链反应-聚丙烯酰胺凝胶电泳(PCR-PAGE)法对277份河南汉族样本的线粒体DNA RegionⅤ进行多态性分析,并随机选取部分DNA扩增产物进行测序予以确认。结果在河南汉族群体中发现标准型和缺失型2种多态类型,缺失型多态(即9 bp缺失)出现频率为15.88%。结论河南省汉族群体DNA RegionⅤ缺失型多态基因频率较低,与其他民族或人种有一定差异。展开更多
In order to reveal the changes of vegetation in southern China since the Last Glacial Maximum, we have established high-resolution time scales and palynological sequences of borehole profiles by drilling cores in some...In order to reveal the changes of vegetation in southern China since the Last Glacial Maximum, we have established high-resolution time scales and palynological sequences of borehole profiles by drilling cores in some weak areas of the research to restore vegetation changes over the past 20,000 years on the basis of previous work. This paper gives the vegetation zoning maps of 18, 9 and 6 ka BP respectively in southern China, and describes the distribution characteristics of plants in different zones/subzones. The results show that the vegetation zonations around 18 ka BP were significantly different from that at present.It appeared in turn with Cold-temperate coniferous forest and alpine meadow steppe zone, and Temperate mixed coniferous and broad-leaved forest zone/warm temperate deciduous broad-leaved forest zone from northwest to southeast in the west, and Temperate mixed coniferous and broad-leaved forest zone, Warm temperate deciduous broad-leaved forest zone, and Northern subtropical mixed evergreen and deciduous broad-leaved forest zone from north to south in the central and east. The vegetation distribution around 9 ka BP changed distinctively. Except that the northwest part was located in Mountain temperate mixed coniferous and broad-leaved forest zone, the vegetation in other areas occurred in turn with North subtropical mixed evergreen and deciduous broad-leaved forest subzone, Mid-subtropical typical evergreen broad-leaved forest subzone, and South subtropical monsoon evergreen broad-leaved forest subzone/Tropical seasonal rainforest and rainforest zone from north to south.There was little change in the appearance of vegetation zonations between 6 and 9 ka BP, but the northern edge of each vegetation belt moved a little northward, reflecting that the overall climate became warmer around 6 ka BP. The vegetation changes in southern China over the past 20,000 years were largely driven by environmental changes. Climate change was the main factor affecting the vegetation distribution. The impact of human activities became more and more remarkable in the later period. In the lower reaches of the Yangtze River and the delta region, sea level changes also influenced the vegetation distribution.展开更多
文摘目的:研究广西不同地区壮族的mtDNA Region V区的遗传多态性及其遗传结构的相关性。方法:采用PCR-PAGE和直接测序广西地区壮族样本线粒体DNA Region V区进行序列分析。结果:在广西壮族样本中只发现标准型和短型两种多态。广西壮族的9-bp缺失频率平均为18.94%;其中广西南宁、柳州、百色和河池等地区的壮族9-bp缺失频率分别为22.22%、14.58%、12.50%和22.45%(P>0.05)。结论:来自广西不同地区的壮族人群的9-bp缺失频率虽有所不同,但没有明显差异。
文摘旨在研究中国广东省部分地区汉族人群线粒体DNA Region Ⅴ 9bp序列缺失情况。采用PCR-PAGE和直接测序法对3个群体144份样本mtDNA Region Ⅴ进行序列分析。结果只检测到标准型和短型(即9bp缺失)两种多态。广东汉族人群的平均缺失频率为21.5%,广州、东莞和湛江汉族人群的缺失频率依次为20.8%、19.2%和25.0%。由此得出,广东汉族人群mtDNA9bp缺失频率较高,与其它地区汉族群体存在一定的差异。
文摘内脂素(Visfatin)是脂肪细胞因子家族的新成员,主要由内脏脂肪组织产生.研究表明内脂素具有类胰岛素样作用.在检测固始鸡-安卡鸡资源群体3代(亲本,F1,F2)964只鸡Visfatin基因9bp插入/缺失(9 bp 'TAACCTGTG' insertion-deletion)多态的过程中,发现其杂合子的变性和非变性聚丙烯酰胺胶上除2条同源双链DNA(282bp和273bp)外有2条未知条带(命名为A和B).A,B条带经回收、二次PCR、再次聚丙烯酰胺凝胶电泳及DNA测序表明:Visfatin基因第10内含子中9bp insertion-deletion突变杂合子的PCR产物中,本身包含2种同源双链DNA片段和2种异源双链DNA片段,不需要经过额外的变性、退火处理,其PCR产物可以直接进行突变检测,在229个杂合突变中异源双链DNA的检出率为100%.因此,通过异源双链DNA这一标示物作为基因分型时的依照或者参考,建立适当的异源双链DNA分析法可进行基因中几个核苷酸插入/缺失多态的检测.
文摘根据中国西北干旱区巴里坤湖BLK-1剖面沉积物的地化元素分析,采用SPSS因子分析法提取了对沉积环境变化敏感的地化元素和氧化物组分.在R、Q型因子分析的基础上,结合沉积物的硅铁铝率(SiO2/(Al2O3+Fe2O3))、淋失系数(SiO2/(MgO+K2O))、CaO/MgO以及腐殖化度等指标,提取出古气候环境信息.初步研究结果表明:近9.0 cal ka BP以来,巴里坤湖地区气候环境仍以干旱化为主,全新世期间出现过多次不同程度的干湿变化,经历了5个气候阶段:9.0—7.5 cal ka BP期间,气候干旱;7.5—5.8 cal ka BP期间,气候温暖湿润,为研究区全新世最佳适宜期;5.8—3.0 cal ka BP期间,气候干旱;3.0—1.0 cal ka BP期间,气候湿润;1.0—0 cal ka BP期间,气候干旱.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences (Grant Nos. XDA05120100 & XDB26000000)the National Natural Science Foundation of China (Grant Nos. 41771219, 41371207 & 41472010)
文摘In order to reveal the changes of vegetation in southern China since the Last Glacial Maximum, we have established high-resolution time scales and palynological sequences of borehole profiles by drilling cores in some weak areas of the research to restore vegetation changes over the past 20,000 years on the basis of previous work. This paper gives the vegetation zoning maps of 18, 9 and 6 ka BP respectively in southern China, and describes the distribution characteristics of plants in different zones/subzones. The results show that the vegetation zonations around 18 ka BP were significantly different from that at present.It appeared in turn with Cold-temperate coniferous forest and alpine meadow steppe zone, and Temperate mixed coniferous and broad-leaved forest zone/warm temperate deciduous broad-leaved forest zone from northwest to southeast in the west, and Temperate mixed coniferous and broad-leaved forest zone, Warm temperate deciduous broad-leaved forest zone, and Northern subtropical mixed evergreen and deciduous broad-leaved forest zone from north to south in the central and east. The vegetation distribution around 9 ka BP changed distinctively. Except that the northwest part was located in Mountain temperate mixed coniferous and broad-leaved forest zone, the vegetation in other areas occurred in turn with North subtropical mixed evergreen and deciduous broad-leaved forest subzone, Mid-subtropical typical evergreen broad-leaved forest subzone, and South subtropical monsoon evergreen broad-leaved forest subzone/Tropical seasonal rainforest and rainforest zone from north to south.There was little change in the appearance of vegetation zonations between 6 and 9 ka BP, but the northern edge of each vegetation belt moved a little northward, reflecting that the overall climate became warmer around 6 ka BP. The vegetation changes in southern China over the past 20,000 years were largely driven by environmental changes. Climate change was the main factor affecting the vegetation distribution. The impact of human activities became more and more remarkable in the later period. In the lower reaches of the Yangtze River and the delta region, sea level changes also influenced the vegetation distribution.
