国内一些研究所和科研机构从国外购入大量Fortran程序源代码,为拓宽其应用领域,需要把Fortran源程序翻译成C/C++程序,即实现Fortran应用程序二次开发利用。针对开发Fortran 95 TO C/C++翻译器的具体功能要求,详细介绍了翻译器的实现方法...国内一些研究所和科研机构从国外购入大量Fortran程序源代码,为拓宽其应用领域,需要把Fortran源程序翻译成C/C++程序,即实现Fortran应用程序二次开发利用。针对开发Fortran 95 TO C/C++翻译器的具体功能要求,详细介绍了翻译器的实现方法,并对翻译器进行了需求及可行性分析,对目标语言的选择、总体设计方案、功能与结构模块划分、软件流程图等进行了分析说明。展开更多
Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was trans...Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C.The experiment group was divided into blank control group,empty vector transfected group and MicroRNA-lOb transfected group.Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4mRNA expression.Proliferations of cells were detected by cell proliferation assay,invasion of the delected the cell Transwell experiments,the expression of KLF4 protein was detected in Western blotting cells.Results:The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection,invasion and migration ability enhancement,by comparison,there are statistically significant differences in the blank control group and negative control group(P<0.05);the expression of MicroRNA-lOb piasmid transfection group KLF4protein decreased,the difference was statistically significant(P<0.05);reduce the expression of MicroRNA-l0b piasmid transfection group KLF4mRNA,but no significant differences(P>0.05).Conclusions:MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.展开更多
目的:研究温胆汤对精神分裂症模型大鼠行为学及其海马神经元突触后致密蛋白95(PSD-95)、生长相关蛋白43(GAP-43)、JNK相互作用蛋白3(JIP-3)、连接蛋白43(Cx-43)表达的影响。方法:将SD大鼠随机分为:正常对照组、模型对照组、氯氮平0.02 g...目的:研究温胆汤对精神分裂症模型大鼠行为学及其海马神经元突触后致密蛋白95(PSD-95)、生长相关蛋白43(GAP-43)、JNK相互作用蛋白3(JIP-3)、连接蛋白43(Cx-43)表达的影响。方法:将SD大鼠随机分为:正常对照组、模型对照组、氯氮平0.02 g/kg组、温胆汤10、20、40 g/kg组。正常对照组和模型对照组分别灌胃生理盐水,各组分别灌胃相应药物,1次/d,连续给药21 d。末次给药2 h后,除正常对照组外,其余各组一次性腹腔注射MK-8010.6 mg/kg,以诱发精神分裂症模型。造模后,观察记录各组大鼠的行为学改变和刻板行为评分,旷场实验评价大鼠滞留时间及穿越次数;RT-q PCR法检测大鼠海马组织Psd95、Gap43、Jip3、Cx43 m RNA表达;WB法检测大鼠海马组织PSD-95、GAP-43、JIP-3、Cx-43蛋白表达。结果:与正常对照组比较,模型对照组大鼠刻板行为评分显著升高,在中央区滞留时间及穿越次数显著下降(P<0.01);其海马组织Psd95、Gap43、Jip3、Cx43 m RNA和蛋白表达显著下调(P<0.01);与模型对照组比较,温胆汤10、20、40 g/kg组均能明显降低大鼠刻板行为评分,明显升高中央区滞留时间及穿越频次(P<0.05或P<0.01);能明显上调海马组织Psd95、Gap43、Jip3、Cx43 m RNA和蛋白的表达(P<0.05或P<0.01)。结论:温胆汤可能通过上调PSD-95、GAP-43、JIP-3、Cx-43等突触相关蛋白的表达,从而调控海马神经元突触可塑性,缓解刻板行为和紧张、焦虑等情绪,增强学习和记忆功能,有效改善精神分裂症的认知障碍。展开更多
文摘国内一些研究所和科研机构从国外购入大量Fortran程序源代码,为拓宽其应用领域,需要把Fortran源程序翻译成C/C++程序,即实现Fortran应用程序二次开发利用。针对开发Fortran 95 TO C/C++翻译器的具体功能要求,详细介绍了翻译器的实现方法,并对翻译器进行了需求及可行性分析,对目标语言的选择、总体设计方案、功能与结构模块划分、软件流程图等进行了分析说明。
基金supported by Sichuan Province Natural Science Foundation of China:(c271628)
文摘Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C.The experiment group was divided into blank control group,empty vector transfected group and MicroRNA-lOb transfected group.Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4mRNA expression.Proliferations of cells were detected by cell proliferation assay,invasion of the delected the cell Transwell experiments,the expression of KLF4 protein was detected in Western blotting cells.Results:The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection,invasion and migration ability enhancement,by comparison,there are statistically significant differences in the blank control group and negative control group(P<0.05);the expression of MicroRNA-lOb piasmid transfection group KLF4protein decreased,the difference was statistically significant(P<0.05);reduce the expression of MicroRNA-l0b piasmid transfection group KLF4mRNA,but no significant differences(P>0.05).Conclusions:MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.
文摘目的:研究温胆汤对精神分裂症模型大鼠行为学及其海马神经元突触后致密蛋白95(PSD-95)、生长相关蛋白43(GAP-43)、JNK相互作用蛋白3(JIP-3)、连接蛋白43(Cx-43)表达的影响。方法:将SD大鼠随机分为:正常对照组、模型对照组、氯氮平0.02 g/kg组、温胆汤10、20、40 g/kg组。正常对照组和模型对照组分别灌胃生理盐水,各组分别灌胃相应药物,1次/d,连续给药21 d。末次给药2 h后,除正常对照组外,其余各组一次性腹腔注射MK-8010.6 mg/kg,以诱发精神分裂症模型。造模后,观察记录各组大鼠的行为学改变和刻板行为评分,旷场实验评价大鼠滞留时间及穿越次数;RT-q PCR法检测大鼠海马组织Psd95、Gap43、Jip3、Cx43 m RNA表达;WB法检测大鼠海马组织PSD-95、GAP-43、JIP-3、Cx-43蛋白表达。结果:与正常对照组比较,模型对照组大鼠刻板行为评分显著升高,在中央区滞留时间及穿越次数显著下降(P<0.01);其海马组织Psd95、Gap43、Jip3、Cx43 m RNA和蛋白表达显著下调(P<0.01);与模型对照组比较,温胆汤10、20、40 g/kg组均能明显降低大鼠刻板行为评分,明显升高中央区滞留时间及穿越频次(P<0.05或P<0.01);能明显上调海马组织Psd95、Gap43、Jip3、Cx43 m RNA和蛋白的表达(P<0.05或P<0.01)。结论:温胆汤可能通过上调PSD-95、GAP-43、JIP-3、Cx-43等突触相关蛋白的表达,从而调控海马神经元突触可塑性,缓解刻板行为和紧张、焦虑等情绪,增强学习和记忆功能,有效改善精神分裂症的认知障碍。
