目的探讨乳腺摄取99mTc-MDP与乳腺癌分子表达产物、分子亚型及患者预后的关系。方法收集161例乳腺癌患者乳腺摄取99mTc-MDP级别、分子表达产物、分子亚型等相关信息,并追踪随访患者预后信息,通过χ^2检验分析乳腺摄取99mTc-MDP与雌激素...目的探讨乳腺摄取99mTc-MDP与乳腺癌分子表达产物、分子亚型及患者预后的关系。方法收集161例乳腺癌患者乳腺摄取99mTc-MDP级别、分子表达产物、分子亚型等相关信息,并追踪随访患者预后信息,通过χ^2检验分析乳腺摄取99mTc-MDP与雌激素受体(ER)、孕激素受体(PR)、表皮生长因子受体(HER)-2的关系,采用单因素及多因素生存分析筛选乳腺癌预后影响因素。结果乳腺区摄取99mTc-MDP程度在不同ER、PR、HER-2、Ki-67、腋窝淋巴结(ALN)表达情况及分子亚型中亦存在差异(P<0.05);ALN及乳腺摄取99mTc-MDP的不同情况其中位无进展生存期存在差异(阳性vs阴性:26 vs 75个月χ^2=14.18,P<0.01;1级vs 2级:75 vs 27个月χ^2=13.94,P<0.01);多因素Cox分析结果表明影响乳腺癌预后的因素有乳腺区放射性高摄取(RR=1.65,P<0.01)及ALN阳性(RR=2.284,P<0.01)。结论乳腺摄取99mTc-MDP的不同级别在分子表达及分子亚型存在差异,该因子可能是判断乳腺癌预后因素之一。全身骨显像除了可提供乳腺癌骨转移信息外,有可能为临床提供附加的预后信息。展开更多
Density functional theory method has been employed to investigate the structures of the prototypical technetium-labeled diphosphonate complex 99mTc-MDP, where MDP represents methylenediphosphonic acid. A total of 14 t...Density functional theory method has been employed to investigate the structures of the prototypical technetium-labeled diphosphonate complex 99mTc-MDP, where MDP represents methylenediphosphonic acid. A total of 14 trial structures were generated by allowing for the geometric, conformational, charge, and spin isomerism. Based on the optimized structures and calculated energies at the B3LYP/LANL2DZ level, two stable isomers were determined for the title complex. And they were further studied systematically in comparison with the experimental structure. The basis sets 6-31G＊（LANL2DZ for Tc）, 6-31G＊（cc-pVDZ-pp for Tc）, and DGDZVP have also been employed in combination with the B3LYP functional to study the basis set effect on the geometries of isomers. The optimized structures agree well with the available experimental data, and the bond lengths are more sensitive to the basis set than the bond angles. The charge distributions were studied by the Mulliken population analysis and natural bond orbital analysis. The results reflect a significant ligand-to-metal electron donation.展开更多
文摘目的探讨乳腺摄取99mTc-MDP与乳腺癌分子表达产物、分子亚型及患者预后的关系。方法收集161例乳腺癌患者乳腺摄取99mTc-MDP级别、分子表达产物、分子亚型等相关信息,并追踪随访患者预后信息,通过χ^2检验分析乳腺摄取99mTc-MDP与雌激素受体(ER)、孕激素受体(PR)、表皮生长因子受体(HER)-2的关系,采用单因素及多因素生存分析筛选乳腺癌预后影响因素。结果乳腺区摄取99mTc-MDP程度在不同ER、PR、HER-2、Ki-67、腋窝淋巴结(ALN)表达情况及分子亚型中亦存在差异(P<0.05);ALN及乳腺摄取99mTc-MDP的不同情况其中位无进展生存期存在差异(阳性vs阴性:26 vs 75个月χ^2=14.18,P<0.01;1级vs 2级:75 vs 27个月χ^2=13.94,P<0.01);多因素Cox分析结果表明影响乳腺癌预后的因素有乳腺区放射性高摄取(RR=1.65,P<0.01)及ALN阳性(RR=2.284,P<0.01)。结论乳腺摄取99mTc-MDP的不同级别在分子表达及分子亚型存在差异,该因子可能是判断乳腺癌预后因素之一。全身骨显像除了可提供乳腺癌骨转移信息外,有可能为临床提供附加的预后信息。
基金This work was supported by the National Natural Science Foundation of China （No.20801024 and No.21001055）, the Natural Science Foundation of Jiangsu Province （No.BK2009077）, and the Science Foundation of Health Department of Jiangsu Province （No.H200963）.
文摘Density functional theory method has been employed to investigate the structures of the prototypical technetium-labeled diphosphonate complex 99mTc-MDP, where MDP represents methylenediphosphonic acid. A total of 14 trial structures were generated by allowing for the geometric, conformational, charge, and spin isomerism. Based on the optimized structures and calculated energies at the B3LYP/LANL2DZ level, two stable isomers were determined for the title complex. And they were further studied systematically in comparison with the experimental structure. The basis sets 6-31G＊（LANL2DZ for Tc）, 6-31G＊（cc-pVDZ-pp for Tc）, and DGDZVP have also been employed in combination with the B3LYP functional to study the basis set effect on the geometries of isomers. The optimized structures agree well with the available experimental data, and the bond lengths are more sensitive to the basis set than the bond angles. The charge distributions were studied by the Mulliken population analysis and natural bond orbital analysis. The results reflect a significant ligand-to-metal electron donation.
