The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised t...The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised tumors, and may be a useful animal model for the evaluation of various therapeutic approaches for gliosarcomas. In this study, the 9L/Wistar rat glioma model was produced by intracerebral implantation of 9L^LUC glioma cells syngenic to Fischer 344 （F344） rats. Bioluminescence imaging showed that tumors progressively grew from day 7 to day 21 in 9L^LUC/F344 rats, and tumor regression was found in some 9L^LUC/Wistar rats. Hematoxylin-eosin staining verified that intracranial tumors were gliomas. Immunohistochemistry results demonstrated that no CD4- and CD8-positive cells were found in the syngeneic 9L^LUC/F344 model. However, many infiltrating CD4- and CD8-positive cells were observed within the tumors of the 9L^LUC/Wistar model. Our data suggests that compared with 9L/F344 rats, 9L glioma Wistar rats may not be suitable for evaluating brain glioma immunotherapies, even though the model induced an immune response and exhibited tumor regression.展开更多
Background Treating intramedullary spinal cord gliomas is a big challenge because of limited options, high recurrence rate and poor prognosis. An intramedullary glioma model is prerequisite for testing new treatments....Background Treating intramedullary spinal cord gliomas is a big challenge because of limited options, high recurrence rate and poor prognosis. An intramedullary glioma model is prerequisite for testing new treatments. This paper describes the establishment of a rodent intramedullary glioma model and presents functional progression, neuroimaging and histopathological characterization of the tumour model. Methods Fischer344 rats （n=24） were randomized into two groups. Group 1 （n=16） received a 5 pl intramedullary implantation of 9L gliosarcomal （105） cells. Group 2 （n=8） received a 5 pl intramedullary injection of Dulbecco＇s modified Eagle medium. The rats were anesthetized, the spinous process of the T10 vertebra and the ligamentum flavum were removed to expose the T10-11 intervertebral space and an intramedullary injection was conducted into the spinal cord. The rats were evaluated preoperatively and daily postoperatively for neurological deficits using the Basso, Beattie and Bresnahan scale. High resolution magnetic resonance images were acquired preoperatively and weekly postoperatively. When score equal to 0, rats were sacrificed for histopathological examination. Results Rats implanted with 9L gliosarcoma cells had a statistically significant median onset of hind limb paraplegia at （16.0±0.4） days, compared with rats in the control group in which neurological deficits were absent. Imaging and pathological cross sections confirmed intramedullary 9L gliosarcoma invading the spinal cord. Rats in the control group showed no significant functional, radiological or histopathological findings of tumour. Conclusions Rats implanted with 9L cells regularly develop paraplegia in a reliable and reproducible manner. The progression of neurological deficits, neuroimaging and histopathological characteristics of intramedullary spinal cord gliomas in rats is comparable with the behaviour of infiltrative intramedullary spinal cord gliomas in patients.展开更多
目的：研究钾离子通道阻滞剂四乙胺（T EA ）在体外是否具有抗胶质瘤作用，对胶质瘤细胞是否具有抑制肿瘤细胞增殖的作用。方法四甲基噻唑蓝比色法（MTT 法）观察 TEA对胶质瘤细胞生长、增殖的影响。结果 MTT法发现 TEA可显著抑制胶质...目的：研究钾离子通道阻滞剂四乙胺（T EA ）在体外是否具有抗胶质瘤作用，对胶质瘤细胞是否具有抑制肿瘤细胞增殖的作用。方法四甲基噻唑蓝比色法（MTT 法）观察 TEA对胶质瘤细胞生长、增殖的影响。结果 MTT法发现 TEA可显著抑制胶质瘤细胞株的生长，且呈时间和剂量依赖性。结论 T EA在体外可显著抑制C6与9L胶质瘤细胞株增殖，其作用机制可能和细胞凋亡有关。展开更多
文摘The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised tumors, and may be a useful animal model for the evaluation of various therapeutic approaches for gliosarcomas. In this study, the 9L/Wistar rat glioma model was produced by intracerebral implantation of 9L^LUC glioma cells syngenic to Fischer 344 （F344） rats. Bioluminescence imaging showed that tumors progressively grew from day 7 to day 21 in 9L^LUC/F344 rats, and tumor regression was found in some 9L^LUC/Wistar rats. Hematoxylin-eosin staining verified that intracranial tumors were gliomas. Immunohistochemistry results demonstrated that no CD4- and CD8-positive cells were found in the syngeneic 9L^LUC/F344 model. However, many infiltrating CD4- and CD8-positive cells were observed within the tumors of the 9L^LUC/Wistar model. Our data suggests that compared with 9L/F344 rats, 9L glioma Wistar rats may not be suitable for evaluating brain glioma immunotherapies, even though the model induced an immune response and exhibited tumor regression.
文摘Background Treating intramedullary spinal cord gliomas is a big challenge because of limited options, high recurrence rate and poor prognosis. An intramedullary glioma model is prerequisite for testing new treatments. This paper describes the establishment of a rodent intramedullary glioma model and presents functional progression, neuroimaging and histopathological characterization of the tumour model. Methods Fischer344 rats （n=24） were randomized into two groups. Group 1 （n=16） received a 5 pl intramedullary implantation of 9L gliosarcomal （105） cells. Group 2 （n=8） received a 5 pl intramedullary injection of Dulbecco＇s modified Eagle medium. The rats were anesthetized, the spinous process of the T10 vertebra and the ligamentum flavum were removed to expose the T10-11 intervertebral space and an intramedullary injection was conducted into the spinal cord. The rats were evaluated preoperatively and daily postoperatively for neurological deficits using the Basso, Beattie and Bresnahan scale. High resolution magnetic resonance images were acquired preoperatively and weekly postoperatively. When score equal to 0, rats were sacrificed for histopathological examination. Results Rats implanted with 9L gliosarcoma cells had a statistically significant median onset of hind limb paraplegia at （16.0±0.4） days, compared with rats in the control group in which neurological deficits were absent. Imaging and pathological cross sections confirmed intramedullary 9L gliosarcoma invading the spinal cord. Rats in the control group showed no significant functional, radiological or histopathological findings of tumour. Conclusions Rats implanted with 9L cells regularly develop paraplegia in a reliable and reproducible manner. The progression of neurological deficits, neuroimaging and histopathological characteristics of intramedullary spinal cord gliomas in rats is comparable with the behaviour of infiltrative intramedullary spinal cord gliomas in patients.
文摘目的：研究钾离子通道阻滞剂四乙胺（T EA ）在体外是否具有抗胶质瘤作用，对胶质瘤细胞是否具有抑制肿瘤细胞增殖的作用。方法四甲基噻唑蓝比色法（MTT 法）观察 TEA对胶质瘤细胞生长、增殖的影响。结果 MTT法发现 TEA可显著抑制胶质瘤细胞株的生长，且呈时间和剂量依赖性。结论 T EA在体外可显著抑制C6与9L胶质瘤细胞株增殖，其作用机制可能和细胞凋亡有关。
