A disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13(ADAMTS13) specifically cleaves unusually-large von Willebrand factor(VWF) multimers under high shear stress,and down-regulates VWF function...A disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13(ADAMTS13) specifically cleaves unusually-large von Willebrand factor(VWF) multimers under high shear stress,and down-regulates VWF function to form platelet thrombi.Deficiency of plasma ADAMTS13 activity induces a life-threatening systemic disease,termed thrombotic microangiopathy(TMA) including thrombotic thrombocytopenic purpura(TTP).Children with advanced biliary cirrhosis due to congenital biliary atresia sometimes showed pathological features of TMA,with a concomitant decrease of plasma ADAMTS13 activity.Disappearance of their clinical findings of TTP after successful liver transplantation suggested that the liver is a major organ producing plasma ADAMTS13.In situ hybridization analysis showed that ADAMTS13 was produced by hepatic stellate cells.Subsequently,it was found that ADADTS13 was not merely responsible to development of TMA and TTP,but also related to some kinds of liver dysfunction after liver transplantation.Ischemia-reperfusion injury and acute rejection in liver transplant recipients were often associated with marked decrease of ADAMTS13 and concomitant formation of unusually large VWF multimers without findings of TMA/TTP.The similar phenomenon was observed also in patients who underwent hepatectomy for liver tumors.Imbalance between ADAMTS13 and VWF in the hepatic sinusoid might cause liver damage due to microcirculatory disturbance.It can be called as "local TTP like mechanism" which plays a crucial role in liver dysfunction after liver transplantation and surgery.展开更多
目的:检测含Ⅰ型凝血酶敏感蛋白模体的解整合素样金属蛋白酶(a disintegrin and metalloprotease with thrombospondin motif,ADAMTS)-2及转化生长因子(transforming growth factor,TGF)-β1在肝硬化组织中的表达,探讨ADAMTS-2与TGF-β...目的:检测含Ⅰ型凝血酶敏感蛋白模体的解整合素样金属蛋白酶(a disintegrin and metalloprotease with thrombospondin motif,ADAMTS)-2及转化生长因子(transforming growth factor,TGF)-β1在肝硬化组织中的表达,探讨ADAMTS-2与TGF-β1在肝硬化发生和发展中的作用。方法:收集武汉总医院2010年3月至6月肝硬化门静脉高压症患者的肝组织16例及外伤性肝破裂正常肝组织8例作为正常对照组。采用免疫组织化学及Western印迹法检测ADAMTS-2及TGF-β1在肝硬化组织及正常组织中的表达。结果:免疫组织化学结果显示肝硬化组织中ADAMTS-2和TGF-β1表达较正常肝组织明显增高(P<0.05)。Western印迹分析亦显示ADAMTS-2及TGF-β1在肝硬化组织中表达强度明显高于正常组织(P<0.05),而且两者的表达呈正相关(r=0.862,P<0.01)。结论:ADAMTS-2和TGF-β1可能存在协同致肝纤维化作用。展开更多
目的:通过系统评价与Meta分析探索Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶7(ADAMTS7)基因rs3825807位点单核苷酸的多态性与冠心病发病风险的关联。方法:计算机检索PubMed, Web of Science, Cochrane Library,中国知网,万方,维普...目的:通过系统评价与Meta分析探索Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶7(ADAMTS7)基因rs3825807位点单核苷酸的多态性与冠心病发病风险的关联。方法:计算机检索PubMed, Web of Science, Cochrane Library,中国知网,万方,维普和中国生物医学数据库,以获取ADAMTS7基因rs3825807多态性与冠心病易感性的原始研究。检索时限均为建库至2019年12月6日。由两位研究者独立筛选文献、提取数据并评价纳入研究的偏倚风险后,采用RevMan 5.3软件进行Meta分析。结果:共纳入6个病例-对照研究,观察组包括4 989例病例,对照组包含5 471例。Meta分析结果显示,患者ADAMTS7基因rs3825807多态性与冠心病的发病风险增加有相关性(AA vs, GG:OR=21.07,95%CI:1.61~275.95,P=0.02;AG vs. GG:OR=6.80,95%CI:0.77~60.11,P=0.08;AA+AG vs. GG:OR=13.19,95%CI:1.09~158.97,P=0.04;AA vs. AG+GG:OR=2.39,95%CI:1.44~3.96,P=0.0007;A vs. G:OR=23.44,95%CI:8.19~67.10,P<0.00001)。结论:患者ADAMTS7基因rs3825807多态性是冠心病的发病风险因素之一。受纳入研究数量和质量限制,本研究需更多的高质量临床研究予以验证。展开更多
目的探讨心绞痛患者血清白细胞介素-6受体(interleukin-6 receptor,IL-6R)及含N型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-1(a disintegrin and metalloprotease with thrombospondin type 1 motifs,ADAMTS-1)浓度与冠状动脉粥样硬...目的探讨心绞痛患者血清白细胞介素-6受体(interleukin-6 receptor,IL-6R)及含N型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-1(a disintegrin and metalloprotease with thrombospondin type 1 motifs,ADAMTS-1)浓度与冠状动脉粥样硬化病变程度及冠状动脉斑块稳定性的关系。方法本实验共选取患者223例:心绞痛患者167例,其中不稳定型心绞痛(unstable angina,UA)126例,稳定型心绞痛(stable angina,SA)41例;对照组56例。依据心绞痛患者冠状动脉造影结果进行Gensini评分,分为4个亚组(冠状动脉病变轻度、中度、重度、极重度亚组)。应用酶联免疫吸附试验(ELISA)法检测血清IL-6R、ADAMTS-1浓度,并测定血清总胆固醇、三酰甘油、低密度脂蛋白浓度。结果 UA组IL-6R与ADAMTS-1浓度较SA组明显增高,差异有统计学意义(P<0.05)。心绞痛不同冠状动脉病变程度4个亚组中血清IL-6R、ADAMTS-1浓度比较,差异无统计学意义(P>0.05)。结论外周血IL-6R与ADAMTS-1浓度与冠状动脉病变的严重程度无明显相关性,与冠状动脉粥样硬化斑块的稳定性相关。展开更多
文摘A disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13(ADAMTS13) specifically cleaves unusually-large von Willebrand factor(VWF) multimers under high shear stress,and down-regulates VWF function to form platelet thrombi.Deficiency of plasma ADAMTS13 activity induces a life-threatening systemic disease,termed thrombotic microangiopathy(TMA) including thrombotic thrombocytopenic purpura(TTP).Children with advanced biliary cirrhosis due to congenital biliary atresia sometimes showed pathological features of TMA,with a concomitant decrease of plasma ADAMTS13 activity.Disappearance of their clinical findings of TTP after successful liver transplantation suggested that the liver is a major organ producing plasma ADAMTS13.In situ hybridization analysis showed that ADAMTS13 was produced by hepatic stellate cells.Subsequently,it was found that ADADTS13 was not merely responsible to development of TMA and TTP,but also related to some kinds of liver dysfunction after liver transplantation.Ischemia-reperfusion injury and acute rejection in liver transplant recipients were often associated with marked decrease of ADAMTS13 and concomitant formation of unusually large VWF multimers without findings of TMA/TTP.The similar phenomenon was observed also in patients who underwent hepatectomy for liver tumors.Imbalance between ADAMTS13 and VWF in the hepatic sinusoid might cause liver damage due to microcirculatory disturbance.It can be called as "local TTP like mechanism" which plays a crucial role in liver dysfunction after liver transplantation and surgery.
文摘目的:检测含Ⅰ型凝血酶敏感蛋白模体的解整合素样金属蛋白酶(a disintegrin and metalloprotease with thrombospondin motif,ADAMTS)-2及转化生长因子(transforming growth factor,TGF)-β1在肝硬化组织中的表达,探讨ADAMTS-2与TGF-β1在肝硬化发生和发展中的作用。方法:收集武汉总医院2010年3月至6月肝硬化门静脉高压症患者的肝组织16例及外伤性肝破裂正常肝组织8例作为正常对照组。采用免疫组织化学及Western印迹法检测ADAMTS-2及TGF-β1在肝硬化组织及正常组织中的表达。结果:免疫组织化学结果显示肝硬化组织中ADAMTS-2和TGF-β1表达较正常肝组织明显增高(P<0.05)。Western印迹分析亦显示ADAMTS-2及TGF-β1在肝硬化组织中表达强度明显高于正常组织(P<0.05),而且两者的表达呈正相关(r=0.862,P<0.01)。结论:ADAMTS-2和TGF-β1可能存在协同致肝纤维化作用。
文摘目的:通过系统评价与Meta分析探索Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶7(ADAMTS7)基因rs3825807位点单核苷酸的多态性与冠心病发病风险的关联。方法:计算机检索PubMed, Web of Science, Cochrane Library,中国知网,万方,维普和中国生物医学数据库,以获取ADAMTS7基因rs3825807多态性与冠心病易感性的原始研究。检索时限均为建库至2019年12月6日。由两位研究者独立筛选文献、提取数据并评价纳入研究的偏倚风险后,采用RevMan 5.3软件进行Meta分析。结果:共纳入6个病例-对照研究,观察组包括4 989例病例,对照组包含5 471例。Meta分析结果显示,患者ADAMTS7基因rs3825807多态性与冠心病的发病风险增加有相关性(AA vs, GG:OR=21.07,95%CI:1.61~275.95,P=0.02;AG vs. GG:OR=6.80,95%CI:0.77~60.11,P=0.08;AA+AG vs. GG:OR=13.19,95%CI:1.09~158.97,P=0.04;AA vs. AG+GG:OR=2.39,95%CI:1.44~3.96,P=0.0007;A vs. G:OR=23.44,95%CI:8.19~67.10,P<0.00001)。结论:患者ADAMTS7基因rs3825807多态性是冠心病的发病风险因素之一。受纳入研究数量和质量限制,本研究需更多的高质量临床研究予以验证。
文摘目的探讨心绞痛患者血清白细胞介素-6受体(interleukin-6 receptor,IL-6R)及含N型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-1(a disintegrin and metalloprotease with thrombospondin type 1 motifs,ADAMTS-1)浓度与冠状动脉粥样硬化病变程度及冠状动脉斑块稳定性的关系。方法本实验共选取患者223例:心绞痛患者167例,其中不稳定型心绞痛(unstable angina,UA)126例,稳定型心绞痛(stable angina,SA)41例;对照组56例。依据心绞痛患者冠状动脉造影结果进行Gensini评分,分为4个亚组(冠状动脉病变轻度、中度、重度、极重度亚组)。应用酶联免疫吸附试验(ELISA)法检测血清IL-6R、ADAMTS-1浓度,并测定血清总胆固醇、三酰甘油、低密度脂蛋白浓度。结果 UA组IL-6R与ADAMTS-1浓度较SA组明显增高,差异有统计学意义(P<0.05)。心绞痛不同冠状动脉病变程度4个亚组中血清IL-6R、ADAMTS-1浓度比较,差异无统计学意义(P>0.05)。结论外周血IL-6R与ADAMTS-1浓度与冠状动脉病变的严重程度无明显相关性,与冠状动脉粥样硬化斑块的稳定性相关。
