[Objectives]To study the antioxidant activity of the ethyl acetate extract of Amomum villosum Lour.[Methods]The removal rate of chelated iron ions,hydroxyl radicals,superoxide anion free radicals and DPPH free radical...[Objectives]To study the antioxidant activity of the ethyl acetate extract of Amomum villosum Lour.[Methods]The removal rate of chelated iron ions,hydroxyl radicals,superoxide anion free radicals and DPPH free radicals by the ethyl acetate extract of A.villosum Lour.was determined by UV spectrophotometer.[Results]0.5000μg/mL ethyl acetate extract of A.villosum Lour.had the strongest ability to chelate with ferrous ions and to remove hydroxyl radicals,superoxide anion free radicals and DPPH free radicals.The ability to chelate with ferrous ions was 95.14%,and the removal rate of the above free radicals was 86.217%,81.44%,and 85.16%.[Conclusions]The ethyl acetate extract of A.villosum Lour.had a strong antioxidant effect,and its antioxidant capacity was related to the sample concentration,which provides a theoretical basis for its application in the development of antioxidant skin care products.展开更多
目的:研究阳春砂仁和化橘红对小鼠肝脏CYP3A以及肠道首过效应的影响,以指导临床精细用药。方法:将小鼠分为纯水对照组(10 m L/kg),酮康唑(1.8 mg/kg)、阳春砂仁和化橘红高、低剂量组(30 m L/kg和10 m L/kg)。灌胃给药2次/d,连续3 d。以...目的:研究阳春砂仁和化橘红对小鼠肝脏CYP3A以及肠道首过效应的影响,以指导临床精细用药。方法:将小鼠分为纯水对照组(10 m L/kg),酮康唑(1.8 mg/kg)、阳春砂仁和化橘红高、低剂量组(30 m L/kg和10 m L/kg)。灌胃给药2次/d,连续3 d。以分光光度法测定血中对乙酰氨基酚浓度;用超速离心法分离小鼠肝微粒体;分光光度法检测微粒体中CYP3A活性。结果:以红霉素和氨基比林为底物测定肝脏CYP3A活性时,阳春砂仁和化橘红高、低剂量组与纯水对照组之间均无显著性差异(P>0.05);血中APAP浓度测定结果显示,阳春砂仁和化橘红高、低剂量组显著高于纯水对照组(P<0.01或P<0.05);P-gp偶联的ATP酶活性测定结果显示,阳春砂仁和化橘红高、低剂量组明显低于纯水对照组(P<0.01或P<0.05)。结论:阳春砂仁和化橘红对小鼠肝脏CYP3A的活性均没有抑制或诱导作用,对小鼠肠道P-gp活性具有不同程度的抑制作用。展开更多
Objective:Amomum villosum(AV)is an herb whose dried fruit has been extensively used in modern medicine to treat digestive system diseases such as dysentery,vomiting and abdominal pain.This paper aims to supplement chl...Objective:Amomum villosum(AV)is an herb whose dried fruit has been extensively used in modern medicine to treat digestive system diseases such as dysentery,vomiting and abdominal pain.This paper aims to supplement chloroplast(cp)genomic resources and to be used in phylogenetic studies and identification of AV related plants.Methods:High-throughput sequencing technology was used to determine the complete sequence of the AV cp genome,and the sequence was then compared with three related species.Results:The genome size of AV we obtained was 163,968 bp with an obvious tetrad structure.The AV cp genome was observed to contain 125 unique genes and 81 simple sequence repeat(SSRs)had been determined and the majority of which were adenine–thymine(AT)-rich.Comparative analysis of genome sequence of four ginger plants showed that the atpF,clpP and rpl32 genes are potential markers for identifying Amomum species.Phylogenetic analysis suggested that AV was closely related to A.kravanh and A.compactum.Conclusion:These results have brought useful genetic resources for further identification researches,DNA barcoding,resolving taxonomy and understanding the evolutionary mode of Zingiberaceae cp genome.展开更多
基金Supported by the Innovation and Entrepreneurship Training Planning Project for University Students in Guangxi Autonomous Region(S202210599128X).
文摘[Objectives]To study the antioxidant activity of the ethyl acetate extract of Amomum villosum Lour.[Methods]The removal rate of chelated iron ions,hydroxyl radicals,superoxide anion free radicals and DPPH free radicals by the ethyl acetate extract of A.villosum Lour.was determined by UV spectrophotometer.[Results]0.5000μg/mL ethyl acetate extract of A.villosum Lour.had the strongest ability to chelate with ferrous ions and to remove hydroxyl radicals,superoxide anion free radicals and DPPH free radicals.The ability to chelate with ferrous ions was 95.14%,and the removal rate of the above free radicals was 86.217%,81.44%,and 85.16%.[Conclusions]The ethyl acetate extract of A.villosum Lour.had a strong antioxidant effect,and its antioxidant capacity was related to the sample concentration,which provides a theoretical basis for its application in the development of antioxidant skin care products.
文摘目的:研究阳春砂仁和化橘红对小鼠肝脏CYP3A以及肠道首过效应的影响,以指导临床精细用药。方法:将小鼠分为纯水对照组(10 m L/kg),酮康唑(1.8 mg/kg)、阳春砂仁和化橘红高、低剂量组(30 m L/kg和10 m L/kg)。灌胃给药2次/d,连续3 d。以分光光度法测定血中对乙酰氨基酚浓度;用超速离心法分离小鼠肝微粒体;分光光度法检测微粒体中CYP3A活性。结果:以红霉素和氨基比林为底物测定肝脏CYP3A活性时,阳春砂仁和化橘红高、低剂量组与纯水对照组之间均无显著性差异(P>0.05);血中APAP浓度测定结果显示,阳春砂仁和化橘红高、低剂量组显著高于纯水对照组(P<0.01或P<0.05);P-gp偶联的ATP酶活性测定结果显示,阳春砂仁和化橘红高、低剂量组明显低于纯水对照组(P<0.01或P<0.05)。结论:阳春砂仁和化橘红对小鼠肝脏CYP3A的活性均没有抑制或诱导作用,对小鼠肠道P-gp活性具有不同程度的抑制作用。
基金supported by the National Key R&D Program of China(2017YFC1701104)Guangdong Province Applied Science and Technology R&D Special Fund Project(2015B020234002)。
文摘Objective:Amomum villosum(AV)is an herb whose dried fruit has been extensively used in modern medicine to treat digestive system diseases such as dysentery,vomiting and abdominal pain.This paper aims to supplement chloroplast(cp)genomic resources and to be used in phylogenetic studies and identification of AV related plants.Methods:High-throughput sequencing technology was used to determine the complete sequence of the AV cp genome,and the sequence was then compared with three related species.Results:The genome size of AV we obtained was 163,968 bp with an obvious tetrad structure.The AV cp genome was observed to contain 125 unique genes and 81 simple sequence repeat(SSRs)had been determined and the majority of which were adenine–thymine(AT)-rich.Comparative analysis of genome sequence of four ginger plants showed that the atpF,clpP and rpl32 genes are potential markers for identifying Amomum species.Phylogenetic analysis suggested that AV was closely related to A.kravanh and A.compactum.Conclusion:These results have brought useful genetic resources for further identification researches,DNA barcoding,resolving taxonomy and understanding the evolutionary mode of Zingiberaceae cp genome.
