Application progresses of ultrasound for snakebites and relative complications

Snakebite has become a serious public health problem with high mortality and disability rates.Ultrasound can provide imaging basis for diagnosis and treatment of snakebites and relative complications.The application progresses of ultrasound in snakebites and complications were reviewed in this article.

Review on Preparation and Research of Prescriptions for Snake Bites in Fujian Province

The history of research and preparation of prescriptions for snake bites in Fujian Province was reviewed, and the process of incorporating snake doctors into the system and the gradual establishment of snake bite treatment system in Fujian were explored. The composition of several main snake bite preparations in Fujian was preliminarily compared and analyzed to provide reference for the research and development of similar new preparations at present.

Parallels between Russell's viper(Daboia russelii) and hump-nosed viper(Hypnak species) bites in the central hills of Sri Lanka amidst the heavy burden of unidentified snake bites

Objective:To describe the epidemiology of snake bite in the region and attempt to compare proven Russell’s viper with hump-nosed viper bites.Methods:All snake bite admissions to the Toxicology Unit of Teaching Hospital Peradeniya over three year from 2006 were included. Results:Of the 776 snakebites,665(86%) were unidentified and non-envenomed.Humpnosed viper and Russell’s viper accounted for 55(7%) and 40(5%) bites respectively,of them, incriminated snakes were found in 36(65%) and 19(48%) cases.The cobra bites-5,krait bites-0. The median ages:Russell’s viper bites-41(range 16-66),hump-nosed viper bites-42(range 15- 75).The gender incidence,time of bite(】58%daytime) were similar.In hump-nosed viper bite; upper limb involved in 13(36%),happened at home garden in 22(61%),none in paddy fields.In Russell’s viper bite;6(33%) occurred in paddy fields.Dry bites were similar at 5%.In humpnosed viper bite:local effects 94%,coagulopathy 3%,acute renal failure 3%and one patient died.In Russell’s viper bite;local effects 84%,coagulopathy 53%,neurotoxicity 21%.Abdominal pain occurred only in Russell’s viper bites 10(53%).Conclusions:Overwhelming numbers of unidentified,non-envenomed snakebites are common in the central hills.Some distinctive differences were observed between Russell’s viper and hump-nosed viper bites.

Placental Abruption Following Snakebites Envenomation: A Case Report and Literature Review

Background: Snakebite envenomation in pregnancy is uncommon. It can lead to a poor outcome in both the mother and the fetus. We describe our approach to envenomation in pregnancy based on the currently available evidence. Case: We reported two case of snakebite in the third trimester of pregnancy having caused placental abruption with expelling a fresh still born baby and a live baby. In both cases, consumption coagulopathy occurred. managed by polyvalent anti-snake and blood transfusion. Their investigations became normal and they were discharged of hospitalization. Conclusion: Snakebite envenomation in pregnant is a maternal and fetal emergency. Treatment must be quick and well adapted.

Modelling the spatial distribution of snake species in northwestern Tunisia using maximum entropy(Maxent) and Geographic Information System(GIS)

We used GIS and maximum entropy to predict the potential distribution of six snake species belong to three families in Kroumiria（Northwestern Tunisia）： Natricidae（Natrix maura and Natrix astreptophora）, Colubridae（Hemorrhois hippocrepis, Coronella girondica and Macroprotodon mauritanicus）, and Lamprophiidae（Malpolon insignitus）. The suitable habitat for each species was modelled using the maximum entropy algorithm, combining presence field data（collected during 16 years：2000–2015） with a set of seven environmental variables（mean annual precipitation, elevation, slope gradient,aspect, distance to watercourses, land surface temperature and normalized Differential Vegetation Index. The relative importance of these environmental variables was evaluated by jackknife tests and the predictive power of our models was assessed using the area under the receiver operating characteristic. The main explicative variables of the species distribution were distance from streams and elevation, with contributions ranging from 60 to 77 and from 10 to 25%,respectively. Our study provided the first habitat suitability models for snakes in Kroumiria and this information can be used by conservation biologists and land managers concerned with preserving snakes in Kroumiria.

The Analysis of the Treatment of Rhabdomyolysis by Snake Bites

Objective: To explore the treatment measure of rhabdomyolysis caused by snake bites, and provide guidance for further prevention and control of snake bites. Methods: To retrospectively analyze the 841 cases of serpentine bites in our hospital from January 2006 to July 2017;127 cases of patients with rhabdomyolysis syndrome were screened out, and their clinical manifestations, laboratory results, related treatment and prognosis were analyzed. Results: 127 cases patients were rhabdomyolysis out of 841 cases, 28 cases patients developed acute renal failure;treatment measures included local wound treatment, application of tetanus antitoxin, anti-snake venom serum, anti-infection, fluid “hydration” and diuretic, alkaline urine and hybrid renal replacement therapy. 112 cases were cured, improved conditions in 9 cases, 3 cases of death, 3 cases uncured. The improvement rate of is 95.26% after treatment. Conclusion: The rhabdomyolysis had a high incidence in snake bite patients, severe cases often developed acute renal failure. Serum myopsin and related biochemical blood tests could be used to diagnose rhabdomyolysis quickly. Combined treatment methods are the main measure to increase the cured rate and decrease the death rate.

TAT、TM、PIC、t-PAIC与重型血液毒毒蛇咬伤中毒患者DIC的相关性及预测价值

目的评估凝血酶抗凝酶复合物(TAT)、血栓调节蛋白(TM)、纤溶酶-抗纤溶酶复合物(PIC)和组织型纤溶酶原激活抑制复合物(t-PAIC)与重型血液毒毒蛇咬伤中毒后弥散性血管内凝血(DIC)的临床相关性及预测价值。方法连续纳入2019年4月至2023年4月收治的重型血液毒毒蛇咬伤中毒患者作为研究对象,共132例。依据住院期间是否出现DIC分为观察组(发生DIC,n=37)及对照组(未发生DIC,n=95)。检测2组血浆TAT、TM、PIC、t-PAIC浓度。应用二元、无分类协变量的非条件Logistic回归分析TAT、TM、PIC、t-PAIC浓度与重型血液毒毒蛇咬伤中毒后DIC的临床相关性,建立受试者工作特征(ROC)曲线分析TAT、TM、PIC、t-PAIC对重型血液毒毒蛇咬伤中毒后DIC的预测能力。结果观察组TAT、TM、PIC、t-PAIC显著高于对照组(P<0.05)。二元、无分类协变量的非条件Logistic回归分析显示,TAT[OR=1.517(95%CI:1.155,1.879)]、TM[OR=1.647(95%CI:1.108,2.186)]、PIC[OR=3.989(95%CI:2.986,4.992)]、t-PAIC[OR=1.111(95%CI:0.854,1.368)]是重型血液毒毒蛇咬伤中毒患者发生DIC的危险因素(P<0.05)。ROC曲线分析显示,TAT、TM、PIC、t-PAIC是预测重型血液毒毒蛇咬伤中毒患者DIC的有效指标(P<0.05),其曲线下面积(AUC)分别为0.865(95%CI:0.790,0.939)、0.771(95%CI:0.673,0.870)、0.847(95%CI:0.804,0.889)、0.680(95%CI:0.573,0.787),联合预测效能更优异(P<0.001),AUC为0.904(95%CI:0.875,0.933)。结论TAT、TM、PIC和t-PAIC检测对判断重型血液毒毒蛇咬伤中毒患者是否发生DIC有重要参考价值,可以较好地评估患者凝血功能状态,4个指标联合预测DIC的效能更优。

Clinicopathological spectrum of snake bite-induced acute kidney injury from India

AIM To study the clinico-pathological spectrum of snake bite-induced acute kidney injury(AKI).METHODS A retrospective study of patients admitted at Indira Gandhi Medical College Hospital,Shimla with snake bite-induced AKI from July 2003 to June 2016.Medical records were evaluated for patient's information on demographic,clinical characteristics,complications and outcome.Outcomes of duration of hospital stay,requirement for intensive care unit support,treatment with dialysis,survival and mortality were analyzed.The survival and non survival groups were compared to see the difference in the demographic factors,clinical characteristics,laboratory results,and complications.In patients subjected to kidney biopsy,the findings of histopathological examination of the kidney biopsies were also analyzed.RESULTS One hundred and twenty-one patients were diagnosed with snake bite-induced AKI.Mean age was 42.2 ± 15.1 years and majority(58%) were women.Clinical details were available in 88 patients.The mean duration of arrival at hospital was 3.4 ± 3.7 d with a range of 1 to30 d.Eighty percent had oliguria and 55% had history of having passed red or brown colored urine.Coagulation defect was seen in 89% patients.The hematological and biochemical laboratory abnormalities were:Anemia(80.7%),leukocytosis(75%),thrombocytopenia(47.7%),hyperkalemia(25%),severe metabolic acidosis(39.8%),hepatic dysfunction(40.9%),hemolysis(85.2%) and rhabdomyolysis(68.2%).Main complications were:Gastrointestinal bleed(12.5%),seizure/encephalopathy(10.2%),hypertension,pneumonia/acute respiratory distress syndrome(ARDS) and disseminated intravascular coagulation(9.1% each),hypotension and multi organ failure(MOF)(4.5% each).Eighty-two percent patients required renal replacement therapy.One hundred and ten(90.9%) patient survived and 11(9.1%) patients died.As compared to the survival group,the white blood cell count(P = 0.023) and bilirubin levels(P = 0.006) were significant higher and albumin levels were significantly lower(0.005) in patients who died.The proportion of patients with pneumonia/ARDS(P = 0.001),seizure/encephalopathy(P = 0.005),MOF(P = 0.05) and need for intensive care unit support(0.001) was significantly higher and duration of hospital stay was significantly shorter(P = 0.012) in patients who died.Kidney biopsy was done in total of 22 patients.Predominant lesion on kidney biopsy was acute tubular necrosis(ATN) in 20(91%) cases.In 11 cases had severe ATN and in other nine(41%) cases kidney biopsy showed features of ATN associated with mild to moderate acute interstitial nephritis(AIN).One patient only had moderate AIN and one had patchy renal cortical necrosis(RCN).CONCLUSION AKI due to snake bite is severe and a high proportion requires renal replacement therapy.On renal histology ATN and AIN are common,RCN is rare.

A life threatening scratch on little toe-at most clinical suspicion the essential key in management of snake bite

Snake bites are one among the under reported clinical emergencies from tropical countries.There are variations in clinical presentation of snake bites and its toxic features differ with the species and type of bite.There are lots of controversies in the treatment guidelines which often makes it difficult to manage.We report the case of a severe hemotoxic snake bite who presented to the outpatient service of our hospital with a trivial fool injury.Even though snakebites are familiar clinical situations for an emergency physician from tropics,we report this case as mast are under reported.We also intend to emphasize the excellent outcome of appropriately diagnosed and treated cases of snake bite.

A retrospective analysis of snake envenomation in the intensive care unit of a tertiary care hospital in Delhi

Objective: To evaluate the epidemiology, clinical profile and treatment for patients with snake bite in the intensive care unit of our hospital. Methods: A retrospective analysis of patients with snake bite admitted to the intensive care unit of a tertiary care hospital, North Delhi, was conducted between January 2013 and October 2018. Their demographic profile, time and site of bite, clinical manifestations and time interval between the bite and anti-snake venom administration were recorded. Results: A total number of 102 patients with snake bite were studied, of which 54.9% were males and 45.1% were females. Most patients were presented in the monsoon season and snake bites occurred mainly in the night and early morning. A total of 56.3% of the patients with clinical manifestations received anti-snake venom within 3 h after the snake bite. Most patients presented with painless bites and neuroparalytic features with ptosis that was the most common clinical manifestation (50.9%). Anti-snake venom was administered to 55 patients (53.9%) with an average dose of 42 vials per patient and the mortality was 11.7%. Conclusions: Neuroparalytic krait envenomation accounted for the highest incidence of venomous bites in our study. Early medical treatment with judicious anti snake venom administration and related education is crucial.

基于CiteSpace近10年蛇咬伤治疗研究进展的知识图谱可视化分析

为探讨蛇咬伤治疗研究的发展动态及趋势,检索中国知网2014年1月1日—2023年7月30日蛇咬伤治疗相关文献,使用CiteSpace软件对相关文献进行知识图谱绘制,从发文量、作者、机构、关键词等方面进行可视化分析。结果表明,在纳入分析的503篇文献中,近10年整体发文量呈波动式下降,发文量最多的作者是王万春、龚旭初、罗毅等,发文量较多的机构是江西中医药大学附属医院、广州中医药大学附属新会中医院、安徽中医药大学第一附属医院等,虽然医药大学及所属医院内部联系较密切,但院校之间合作不足。关键词中心性排名依次为临床疗效、预后、毒蛇咬伤、严重程度等,研究热点主要集中在机械通气、血液净化、凝血功能、炎症反应。说明蛇咬伤治疗研究日趋完善,但相应机构之间的合作不紧密,治疗存在地域差异,相关热点研究较少。

Two new species of Curcuma (Zingiberaceae) used as cobra-bite antidotes

Two new species of Curcuma, C. sattayasaii A. Chaveerach ＆ R. Sudmoon and C. zedoaroides A. Chaveerach ＆ T. Tanee with rhizomes traditionally used for many decades as cobra-bite antidotes are described and illustrated. Curcuma sattayasaii is similar to C. longa L., but differs in rhizome horizontally branching on ground; coma bracts pinkish-white or pinkish-pale green; corolla pale yellow with orange tip; labellum pale orange with an orange central band; anther crest very short, broadly ovate, wider than long. Curcuma zedoaroides is similar to C. zedoaria （Christm.） Roscoe, but differs in rhizome branching pattern; the protruding secondary rhizomes curved down; blades oblong to oblong-lanceolate; peduncle glabrous; fertile and coma bracts glabrous; corolla lobes pale yellow to white, lateral lobe ovate, dorsal lobe broadly ovate. The new taxa have been found in a village of Khon Kaen Province, Northeastern Thailand.

Hump nosed viper bite in Sri Lanka-descriptive observational study of 1543 cases

Objective:To identify the clinical manifestations of hump nosed viper envenomation and to recognize the available treatment methods to prevent complications.Methods:Descriptive observational study involving a series of 1 543 patients admitted with hump nosed viper bite to 5 major hospitals in Sri Lanka was conducted.Data collection was done consecutively during February 1990 and February 2008.Except Hypnale,identification of the biting snake was made by the corresponding author after visual examination of the dead or live snakes,which were brought to hospital.Results:Sixty seven(4.34%) patients developed systemic effects and two (0.1%) patients died due to effects of envenomation or complications of treatment.Systemic effects varied from coagulopathy,nephropathy to some neurological manifestations.Fifty nine (3.8%) patients had only coagulopathy and they received either,intravenous isotonic saline to ensure adequate urine out put i.e.0.5 mL/kg /hour or 15 mL/kg of fresh frozen plasma(FFP). None of the patients that had coagulopathy developed renal failure.Contamination of the sample by mildly venomous species of Hypnale may have contributed to the low incidence of systemic complications.Conclusions:It is likely that early hydration with normal saline or FFP can prevent acute renal failure.FFP showed a tendency for early correction of coagulopathy.Role of FFP in hump nosed viper envenomation is worth studying in randomized double blind controlled clinical trials.

Synergistic effect of flavonoids combined with antivenom on neutralisation of Naja naja venom

Objective:To evaluate the neutralizing effects of flavonoids on snake venom toxicity by stand-alone and combinatorial approaches.Methods:Synthetic flavonoids were assessed,either individually or in combination with antivenom,for their neutralization of phospholipase A_(2)(PLA_(2)),protease,antioxidant(DPPH)assay and hemotoxic activity.Molecular docking studies were performed to understand possible binding of flavonoids with Naja naja venom PLA_(2).In vivo studies were carried out to confirm the neutralisation effects using a mouse model.Moreover,inhibition of PLA_(2) was monitored using combinatorial approaches.Results:Among the flavonoids used,quercetin and naringenin inhibited PLA_(2)(56%and 45%),protease(71%and 64%),DPPH scavenging(69.0%and 77.5%)and hemotoxic(70%)activities.Molecular docking studies indicated that the flavonoids bind to the substrate-binding site of PLA_(2)(Cys44 and Tyr63).In vivo studies showed a reduction in the venom toxicity level in the presence of naringenin.Additionally,combinatorial studies using the mixture of flavonoid and anti-venom revealed the possibility of synergistic effect(up to 32%enhancement)in neutralising the venom enzymes.Conclusions:These flavonoids can be used as additives for the treatment of snake bites,which may exert synergistic effects in combination with antivenom and decrease the post-therapeutic effects caused by excessive use of antivenom.

Snakebite Epidemiology in Bangladesh—A National Community Based Health and Injury Survey

Insert Snakebite is a global public health issue, and in majority of cases it is undermined. Tropical and subtropical countries are most effected, Bangladesh being one of them. There is scarcity of countries’ epidemiological situation in relation to snakebite poisoning. This study has looked at the epidemiological status of snakebite poisoning from national representative survey findings. Methods: A nationwide cross-sectional survey was conducted in 12 randomly selected districts of Bangladesh in 2003. A total of 171,366 households were surveyed and information was collected from 819,429 populations by face to face interview. Multi-stage cluster sampling methods were used in this survey and covered urban, rural and slum populations. Results: Annually an estimated 15,372 (10.98/100,000) individuals were bitten by snakes and of them 1709 (1.22/100,000) died every year. Males were found to be most vulnerable with a risk of 1.51 times higher than female. Rural populations were also 10.54 times higher at risk than the populations living in the urban areas. Among the victims 94% were from the poor socio economic conditions. Around 43.9% of the incidences occurred during evening to midnight. Home was found the most common area for snake bite (33.6%), and the Leg was found to be the most common site for biting (63.1%). A total of 96.6% victims sought treatment, of them 61% sought immediate treatment from traditional healing methods. Conclusions: Present incidence of snakebite in Bangladesh has clearly shown that there is emerging public health needs for intervention that can reduce the mortality and burden of the disease in the country.

Persistent fibrinogen deficiency after snake bite: A case report

BACKGROUND Venom-induced consumption coagulopathy(VICC)is characterized by coagulation dysfunction accompanied by decreased coagulation factor activity and fibrinogen(FBG)concentrations.We report a patient with VICC caused by snake bite who manifested persistent FBG deficiency without abnormal coagulation factor activity.This information may be helpful in diagnosing and treating VICC.CASE SUMMARY A 49-year-old man who had been bitten by a snake 13 h previously was admitted to the Emergency Department of our hospital with visible swelling of a finger and a bleeding puncture site.The provisional diagnosis was VICC,this being made based on persistent bleeding from the puncture site and subcutaneous hemorrhage.Laboratory evidence of coagulation abnormalities,including fibrinolysis,and findings on thromboelastography confirmed VICC.He had persistent afibrinogenemia requiring intravenous infusions of cryoprecipitate and fresh frozen plasma,together with continuous large doses of human FBG.After this treatment,the patient’s right upper limb swelling improved significantly and his subcutaneous hemorrhage resolved.All of his abnormal laboratory findings returned to normal by day 25.During 6 months’of follow-up,the patient had no further hemorrhagic events.CONCLUSION Hemorrhagic snake venom can result in coagulation dysfunction characterized by persistent FBG deficiency without abnormal coagulation factor activity.

Living with or Eradicating Poisonous Snakes in Densely Populated Caribbean Islands—A Socio-Ecological Challenge for the French West Indies

The lancehead (Bothrops lanceolatus) is a poisonous snake endemic to Martinique, an island in the Lesser Antilles arc. Today, this snake is on the verge of extinction. The recorded number of snakes killed yearly between 1970 and 2002 decreased by 97%. Despite the production of an antivenom in 1993, the local authorities set up a financial reward to encourage the eradication of lancehead snakes. Today the local population still perceives the snake as a threat, due to its fatal venom and its fierce behaviour. The case study of the lancehead in Martinique highlights the need to develop integrated strategies to conserve species of poisonous snakes on densely populated islands. This requires innovative, cross-sectoral strategies that involve decision-makers working along with multi-disciplinary scientists. Approaching the complexity of ecosystems through the socio-ecological prism implies, conversely, linking up the domains of science and technology, life and environmental sciences, and human and social sciences, through interactions based on sharing common

犀角地黄汤治疗五步蛇咬伤的临床效果

目的探讨犀角地黄汤治疗五步蛇咬伤的临床效果。方法选择2020年1月至2023年6月在我院治疗的五步蛇咬伤患者86例作为研究对象。采用随机数字表法将所有患者分为对照组和观察组,每组43例。对照组给予基础治疗及抗蛇毒血清治疗,观察组在对照组治疗基础上给予犀角地黄汤治疗。观察比较两组患者的临床疗效、纤维蛋白原(FIB)、凝血酶时间(TT)、活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、白细胞(WBC)计数、肿瘤坏死因子-a(TNF-a)、高敏C-反应蛋白(hs-CRP)、患肢与健肢周径差、患肢疼痛程度评分及肿胀缓解时间。结果观察组的治疗总有效率明显高于对照组,差异有统计学意义(P<0.05)。两组患者治疗前的FIB、TT、APTT、PT、WBC、TNF-a、hs-CRP、患肢与健肢周径差及患肢疼痛程度评分比较,差异均无统计学意义(均P>0.05)。观察组治疗后的FIB高于对照组,TT、APTT、PT均低于对照组,差异均有统计学意义(均P<0.05)。观察组治疗后的WBC、TNF-a、hs-CRP、患肢与健肢周径差、患肢疼痛程度评分均低于对照组,差异均有统计学意义(均P<0.05)。结论犀角地黄汤治疗五步蛇咬伤可有效缓解患肢肿胀、疼痛程度,减轻炎症反应,改善凝血功能指标,值得临床应用。

细胞外基质:毒蛇咬伤致局部组织损伤的治疗新靶点

毒蛇咬伤具有发病急、病情变化迅速、致残率及死亡率高等特点。毒素螯入对人体的影响分为全身性和局部毒化作用,目前抗蛇毒血清的普及大大降低了毒蛇咬伤的死亡率,但局部组织损伤及遗留的永久性功能障碍仍是亟待解决的问题。研究发现,蛇毒金属蛋白酶、透明质酸酶、磷脂酶等毒素通过干扰细胞外基质(ECM)的降解和重塑参与多种局部病理效应的发生。本文对ECM与毒蛇咬伤局部组织损伤发展过程的相关机制进行综述,以期寻找有效的治疗靶点,为毒蛇咬伤所致局部组织损伤的临床研究和防治工作提供参考和思路。

蛇伤膏外敷治疗毒蛇咬伤火毒证的疗效观察与网络药理学机制研究

目的:观察蛇伤膏外敷治疗毒蛇咬伤火毒证患者的临床疗效,运用网络药理学方法及分子对接技术分析蛇伤膏治疗毒蛇咬伤潜在的作用机制。方法:选取安徽中医药大学第一附属医院入诊的毒蛇咬伤火毒证患者60例,随机分为对照组和试验组,每组30例。对照组采用常规中医适宜技术治疗,试验组在对照组治疗基础上采用蛇伤膏外敷治疗。观察两组患者临床疗效、症状与体征积分、疼痛与肿胀程度评分、中性粒细胞百分比(NEUT%)、凝血酶原时间(PT)、C反应蛋白(CRP)、白细胞介素-6(IL-6)水平;基于药物与疾病数据库筛选蛇伤膏-毒蛇咬伤靶点及共有靶点,利用DAVID平台进行基因本体(GO)功能分析和京都基因与基因组百科全书(KEGG)通路富集分析,运用Cytoscape构建蛋白相互作用网络(PPI)并筛选关键基因与靶点,利用AutoDockPools1.5.7进行分子对接及PyMol可视化。结果:两组患者临床疗效有效率均为100%,试验组治愈率(67%,20/30例)高于对照组(27%,8/30例),差异有统计学意义(P<0.01);与治疗前比较,试验组治疗后症状与体征积分、疼痛与肿胀程度评分、NEUT、PT、CRP、IL-6水平均下降,差异有统计学意义(P<0.01);两组患者治疗后比较,试验组肿胀程度评分、NEUT均低于对照组,差异有统计学意义(P<0.05),临床疗效、疼痛评分、肢体症状与体征评分、PT、CRP、IL-6指标均明显低于对照组,差异有统计学意义(P<0.01);网络药理学分析显示,蛇伤膏-毒蛇咬伤的核心靶点为表皮生长因子(EGF)、表皮生长因子受体(EGFR)、纤连蛋白1(FN1)、白细胞介素-6(IL-6)、基质金属蛋白酶9(MMP9)、转化生长因子β1(TGF-β1)、肿瘤坏死因子(TNF),核心成分包括槲皮素、黄芩素、木犀草素、汉黄芩素等,主要涉及MAP激酶活性的正调节、细胞外空间、蛋白酶结合、AGE/RAGE通路、TNF等生物功能与信号通路,分子对接结果良好。结论:蛇伤膏治疗毒蛇咬伤火毒证效果显著;网络药理学初步探究了蛇伤膏通过多靶点、多通路途径治疗毒蛇咬伤,与临床相关指标具有联系性,为后续研究提供理论与依据。