Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma(DBA/2J)mice

Glaucoma is a leading cause of irreve rsible blindness wo rldwide,and previous studies have shown that,in addition to affecting the eyes,it also causes abnormalities in the brain.However,it is not yet clear how the primary visual cortex(V1)is altered in glaucoma.This study used DBA/2J mice as a model for spontaneous secondary glaucoma.The aim of the study was to compare the electrophysiological and histomorphological chara cteristics of neurons in the V1between 9-month-old DBA/2J mice and age-matched C57BL/6J mice.We conducted single-unit recordings in the V1 of light-anesthetized mice to measure the visually induced responses,including single-unit spiking and gamma band oscillations.The morphology of layerⅡ/Ⅲneurons was determined by neuronal nuclear antigen staining and Nissl staining of brain tissue sections.Eighty-seven neurons from eight DBA/2J mice and eighty-one neurons from eight C57BL/6J mice were examined.Compared with the C57BL/6J group,V1 neurons in the DBA/2J group exhibited weaker visual tuning and impaired spatial summation.Moreove r,fewer neuro ns were observed in the V1 of DBA/2J mice compared with C57BL/6J mice.These findings suggest that DBA/2J mice have fewer neurons in the VI compared with C57BL/6J mice,and that these neurons have impaired visual tuning.Our findings provide a better understanding of the pathological changes that occur in V1 neuron function and morphology in the DBA/2J mouse model.This study might offer some innovative perspectives regarding the treatment of glaucoma.

The Mathematical and Physical Theory of Rational Human Intelligence: Complete Empirical-Digital Properties;Full Electrochemical-Mechanical Model (Part I: Mathematical Foundations)

The design of this paper is to present the first installment of a complete and final theory of rational human intelligence. The theory is mathematical in the strictest possible sense. The mathematics involved is strictly digital—not quantitative in the manner that what is usually thought of as mathematics is quantitative. It is anticipated at this time that the exclusively digital nature of rational human intelligence exhibits four flavors of digitality, apparently no more, and that each flavor will require a lengthy study in its own right. (For more information,please refer to the PDF.)

J^(#)-U_(c)-clean环与强J^(#)-U_(c)-clean环

设R是一个环,如果U(R)=U_(c)(R)+J^(#)(R),则称R是GU_(c)J环;如果对于任意a∈R,都存在g∈U_(c)(R),p^(2)=p∈R,d∈J^(#)(R)使得ag=p+d(且ap=pa),则称R是(强)J^(#)-U_(c)-clean环。GU_(c)J环和J^(#)-U_(c)-clean环分别是GUJ环和GJ-clean环的真推广。文章研究了GU_(c)J环的基本性质,证明了R是GU_(c)J环当且仅当R/J是U_(c)U环且U_(c)(R/J)=(U_(c)(R)+J)/J,R是U_(c)J环当且仅当R是GU_(c)J环且R/J是reduced的。此外,给出了(强)J^(#)-U_(c)-clean环的例子,得到了(强)J^(#)-U_(c)-clean环的性质和一些等价刻画,证明了若R是一个交换环,则R是GJ-clean环当且仅当存在整数n≥1使得T_(n)(R)是GJ-clean环,当且仅当存在整数n≥2使得T_(n)(R)是J^(#)-U_(c)-clean环。进一步地,研究了强J^(#)-U_(c)-clean环的Morita不变性。

Study of Type-B QPOs Observed in Black Hole X-Ray Binary Swift J1728.9-3613

We report the detection of type-B quasi-periodic oscillation(QPO)of the black hole X-ray binary Swift J1728.9-3613 observed by NICER during the 2019 outburst.A type-B QPO was observed for the first two days and it disappeared as flux increased,but again appeared at∼7.70 Hz when flux was dramatically decreased.The source was found in the soft intermediate state during these observations.We further studied the energy dependence of the QPO.We found that QPO was observed only for a higher energy range implying that the origin of QPO is possibly due to the corona emitting higher energy photons by the inverse Compton process.The variation of spectral parameters can be explained with the disk truncation model.The fractional rms was found to be monotonically increased with energy.The phase lag spectrum followed the“U-shaped”curve.The rms and phase lag spectrum are modeled and explained with the single-component Comptonization model vkompthdk.

‘Partly'globalized networks and driving mechanism in resource-based state-owned enterprises:A case study of J Group

In the context of economic globalization,while multinational enterprises from developed countries occupy a high-end position in the global value chain,enterprises from developing countries are often marginalized in the world market.In China,resource-based state-owned enterprises(SOEs)are tasked with the mission of safeguarding resource security,and their internationalization development ideas and strategic deployment are significantly and fundamentally different from those of other non-state-owned enterprises and large multinational corporations.This study provides ideas for the globalization policies of enterprises in developing countries.We consider J Group in western China as a case and discuss its productive investment and global production network development from 2010 to 2019.We found that J Group was‘Partly'globalized,and there are multiple core nodes with the characteristics of centralized and decentralized coexistence in the production network;in addition,the overall layout centre shifted to Southeast Asia and China;however,its global production was restricted by the enterprise's investment security considerations,support and restrictions of the home country,political security risk of the host country,and sanctions from the West.These findings provide insights for future research:under the wave of anti-globalization and'internal circulation as the main body',resource SOEs should consider the potential risk of investment,especially keeping the middle and downstream industrial chain in China as much as possible.

A Corpus-Based Comparative Multidimensional Analysis of the Two English Translations of Luoyang Jialan Ji

By using the MD approach, this study aims at making a comparison between the lexical features in W. J. F. Jenner’s and Wang Yitong’s translations of Luoyang Jialan Ji (547 AD) from a multidimensional perspective. Statistics show that both the two translations belong to the General Narrative Exposition Register. However, there are significant discrepancies between the two versions in Dimension 1 “Involved versus Informational Production”, Dimension 4 “Overt Expression of Persuasion” and Dimension 5 “Abstract versus Non-Abstract Information”. The differences in Dimension 2 “Narrative versus Non-Narrative Concerns” and Dimension 3 “Explicit versus Situation-Dependent Reference” are not that significant. It is found that in Dimension 1, the information density of Wang’s version is much higher than that of Jenner’s. In Dimension 4, Wang’s version contains more overt persuasive effort than Jenner’s. In Dimension 5, the positive and negative contrast between the two versions indicates that Wang’s version provides information in a more abstract way, whereas Jenner’s in a relatively non-abstract way. Combined with typical examples in the two translation versions, this study is hoped to help better understand the translations of Luoyang Jialan Ji and conduct future studies on the translation styles of the two translators.

Spin Evolution of the Magnetar SGR J1935+2154

Fast radio bursts(FRBs)are short pulses observed in radio frequencies usually originating from cosmological distances.The discovery of FRB 200428 and its X-ray counterpart from the Galactic magnetar SGR J1935+2154suggests that at least some FRBs can be generated by magnetars.However,the majority of X-ray bursts from magnetars are not associated with radio emission.The fact that only in rare cases can an FRB be generated raises the question regarding the special triggering mechanism of FRBs.Here we report long time spin evolution of SGR J1935+2154 until the end of 2022.According to v and v,the spin evolution of SGR J1935+2154 could be divided into two stages.The first stage evolves relatively steady evolution until 2020 April 27.After the burst activity in2020,the spin of SGR J1935+2154 shows strong variations,especially for v.After the burst activity in 2022October,a new spin-down glitch with△v/v=(-7.2±0.6)×10^(-6)is detected around MJD 59876,which is the second event in SGR J1935+2154.At the end,spin frequency and pulse profile do not show variations around the time of FRB 200428 and radio bursts 221014 and 221021,which supply strong clues to constrain the trigger mechanism of FRBs or radio bursts.

Experiencing the Abject Female Body and Writing the Female Self:Body Narrative of In the Heart of the Country

In the Heart of the Country,the second novel by J.M.Coetzee,has been traditionally read as a disembodied writing that focuses on the problem of writing per se instead of the reality.This paper contends that the work is actually a body narrative that explores the visceral pain suffered by Magda whose infertile body impedes her being a qualified subject.As the heroine in a postcolonial novel,Magda is not just a body that is restricted and constructed by the politics of the body,but also a thinking and writing body that consciously questions and resists the gendered bodily norms under whose yardstick her subjecthood is barred.Written in the metafictional manner,Magda’s narrative of the body is not just a record of her corporeal experience,but also a self-conscious negotiation with,and challenge of,the bodily norms under whose yardstick her body has been debased or,in terms of Judith Butler,abjected.Taking her female bodily experience as the starting point,Magda writes a feminine text that values passion,fluidity and non-linearity to disrupt the patriarchal discourse underpinned with logical reasoning.The feminine body narrative endeavors to achieve a new way of communication through which a reciprocal cross race/gender relationship might be established.

A Possible γ-Ray Pulsation from PSR J1740-5340B in the Globular Cluster NGC 6397

Recently, a new radio millisecond pulsar(MSP) J1740-5340B, hosted in the globular cluster(GC) NGC 6397,was reported with a 5.78 ms spin period in an eclipsing binary system with a 1.97 days orbital period. Based on a modified radio ephemeris updated by tool tempo2, we analyze the ~15 yr γ-ray data obtained from the Large Area Telescope on board the Fermi Gamma-ray Space Telescope and detect PSR J1740-5340B's γ-ray pulsation at a confidence level of ~4σ with a weighted H-test value of ~26. By performing a phase-resolved analysis, the γ-ray luminosity in on-pulse interval of PSR J1740-5340B is L_(γ)~ 3.8 × 10^(33) erg s^(-1) using NGC 6397's distance of 2.48 kpc. And γ-rays from the on-pulse part of PSR J1740-5340B contribute ~90% of the total observed γ-ray emissions from NGC 6397. No significant γ-ray pulsation of another MSP J1740-5340A in the GC is detected.Considering that the previous four cases of MSPs in GCs, more data in γ-ray, X-ray, and radio are encouraged to finally confirm the γ-ray emissions from MSP J1740-5340B, especially starving for a precise ephemeris.

基于PACAP-cAMP-PKA信号通路探讨J型针刀调节神经源性膀胱逼尿肌的效应机制

目的:探讨J型针刀通过PACAP-cAMP-PKA信号通路改善脊髓损伤后神经源性膀胱大鼠逼尿肌的作用机制。方法:36只SD雄性大鼠随机分为6组:空白组、假手术组、模型组、J型针刀治疗组、治疗+Bupivacaine抑制剂组、治疗+H-89抑制剂组,各6只。采用脊髓横断法制作神经源性膀胱模型。空白组为正常SD大鼠;假手术组为切开相关部位组织,无脊髓切断;其余4组予以手术脊髓切断造模。J型针刀治疗组造模后第19天予J型针刀针刺大鼠次髎穴,2 d 1次,共治疗7次。干预结束后各组行HE染色观察膀胱逼尿肌组织形态变化,Elisa检测膀胱逼尿肌组织中c AMP、PKA含量,免疫组化检测膀胱逼尿肌组织中PACAP38蛋白表达,Western blot检测膀胱逼尿肌组织中PKA、p-MLC蛋白表达。结果:HE染色结果显示,与空白组相比,模型组中膀胱逼尿肌组织严重坏死,可见大量炎症细胞浸润;与模型组相比,J型针刀治疗组、治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组均有不同程度坏死组织修复情况。免疫组化检测PACAP38蛋白表达在模型组中表达量最低,针刀治疗之后,PACAP38表达量均不同程度上调;与J型针刀治疗组相比,治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组PACAP38蛋白表达均不同程度下调(P<0.05),但高于模型组(P<0.05)。Elisa检测结果显示,cAMP、PKA表达量模型组显著下调(P<0.05),J型针刀治疗组与模型组比显著上调(P<0.05),治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组比无显著差异(P>0.05)。WB结果显示:与空白组相比,模型组PKA、p-MLC蛋白表达上调;针刀治疗组表达量下调,其中PKA蛋白表达有显著性(P<0.05),p-MLC蛋白表达无显著性(P>0.05)。与J型针刀治疗组比,PKA蛋白表达量在治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组中无显著差异(P>0.05),p-MLC蛋白表达量显著下降(P<0.05)。结论:J型针刀针刺次髎穴可改善脊髓损伤后神经源性膀胱大鼠膀胱功能,其机制与J型针刀上调膀胱逼尿肌中PACAP38、cAMP、PKA表达,激活PACAP-cAMP-PKA信号通路,从而促进逼尿肌舒张有关。

Adaptive Features of the Leaf of <i>Juno vicaria</i>(Vved.) T. Hall &Seisums in Different Ecological Conditions of Uzbekistan

The anatomical structure of the leaf of the Central Asian endemic species of Juno vicaria, growing in two different ecological conditions (Baysun Mountains of Surkhandary region and Tashkent Botanical Garden of Uzbekistan) was analyzed and diagnostic signs were determined. Also identified are adaptive mesomorphic and xeromorphic signs from an environmental point of view. A species that grows in natural habitat is dominated by more xeromorphic signs: a thickened outer wall of the epidermis;numerous epidermal, spongy cells;submerged stomata;small, multi-row sclerenchymal and collenchymal cells, which indicate a more xeromorphic and adaptability, as well as the wide distribution of this species to the natural growing conditions. In a species growing under the conditions of introduction of the Tashkent Botanical Garden, more mesomorphic features predominate: thin-walled, large, few epidermal, spongy cells;not immersed stomata;large non multi-row sclerenchymal and collenchymal cells, which show that the species is adapted to altered habitat conditions.

鸡DCAF1生物信息学分析及对J亚群禽白血病病毒复制的影响

【目的】为分析鸡损伤特异性DNA结合蛋白和泛素连接酶复合物骨架蛋白库林4相关因子1(DDB1and CUL4associatedfactor1,DCAF1)蛋白的生物学特性,构建DCAF1基因的真核表达载体,并初步探究其对J亚型禽白血病病毒(ALV-J)复制的影响。【方法】在NCBI中下载相关物种DCAF1蛋白氨基酸序列,采用Mega11.0软件构建系统进化树;利用ProtParam、ProtScale、TMHMM等在线程序分析鸡DCAF1蛋白的氨基酸序列,预测其理化性质、保守结构域及二、三级结构和互作蛋白等生物信息;构建鸡DCAF1基因重组真核表达载体,通过实时荧光定量PCR和Westernblotting技术检测ALV-J感染对DCAF1mRNA和ALV-JEnv蛋白表达水平的影响及过表达DCAF1对ALV-J复制的影响。【结果】鸡DCAF1蛋白由1496个氨基酸组成,其分子质量约为170ku;系统进化树结果表明,鸡与红鸭亲缘关系最近,与斑马鱼亲缘关系最远。生物信息学预测结果表明,鸡DCAF1蛋白无跨膜结构域,无信号肽表达,包含ARM折叠、LisH和WD40重复3个主要结构域与末尾1个酸性结构域,其互作蛋白为DDB1、CUL4A、SAMHD1等。鸡DCAF1蛋白二级结构主要由无规则卷曲和α-螺旋组成,分别占比44.85%和41.18%,延伸链和β-折叠占比次之,分别是9.76%和4.21%。三级结构与二级结构基本一致,建模一致性为93.79%。ALV-J感染24、48h后,DCAF1基因和蛋白表达水平均极显著高于感染0h时(P<0.01);过表达DCAF1后,与感染对照组相比,ALV-JEnv蛋白显著升高(P<0.05)。【结论】鸡DCAF1蛋白由1496个氨基酸组成,分子质量约170ku,属于稳定的亲水蛋白,不含跨膜结构域。本研究成功构建了DCAF1基因真核表达载体。在ALV-J感染的细胞中,DCAF1蛋白和基因表达水平极显著升高,而过表达DCAF1则显著促进ALV-J复制。研究结果为进一步阐明鸡DCAF1的生物学功能及其与ALV-J互作的分子机制奠定了基础。

Searching for the Highest Energy of Pulsation and Critical Luminosity of Swift J0243.6+6124 Observed by Insight-HXMT

Owing to the broad energy coverage of Insight-HXMT in the hard X-ray band,we detected the highest energy of pulsation exceeding 200 keV around the 2017–2018 outburst peak of the first Galactic pulsating ultraluminous X-ray source (PULX) Swift J0243.6+6124,which is the highest energy detected from PULXs to date.We also obtained the highest energy of pulsation of every exposure during the outburst in 2017–2018,and found the highest energy is roughly positively correlated with luminosity.Using our newly developed method,we identified the critical luminosity being 4×10^(38)erg s^(-1) when the main peaks of the low and high energy pulse profiles became aligned,which separates the fan-beam dominated and pencil-beam dominated accretion regimes.Above the critical luminosity,the phase of the main peak shifted gradually from 0.5 to 0.8 until the outburst peak in al energy bands is reached,which is in agreement with the phase shift found previously at low energies.Our result is consistent with what is derived from spectral analysis.

基于SAE J1772协议的车载取电模块设计

阐述一款包含控制与通信的车载取电模块设计,该模块集成电源、采集、驱动及通信的硬件电路,采用AC7811QBFE单片机控制,符合SAE J1772标准,可通过蓝牙实时监控数据。

ATP5J通过TOMM20调节线粒体功能并促进人肝细胞癌细胞转移

目的:探讨ATP合成酶H+转运线粒体F0复合体亚基F6(ATP5J)通过调节肝癌细胞线粒体功能介导细胞骨架重塑影响肝癌细胞转移的作用及其机制。方法:培养人肝细胞癌Li-7细胞株,基因修饰ATP5J表达(过表达和敲减)。使用JC-1染色检测每组细胞线粒体膜电位情况;利用DCFH-DA荧光探针检测Li-7细胞的活性氧(ROS)含量;线粒体ATP荧光探针检测线粒体功能;微丝绿色荧光探针(Actin-Tracker Green-488)检测细胞骨架重塑情况;Transwell检测细胞侵袭能力;Western blot检测ATP5J和线粒体外膜转位酶20(TOMM20)的表达水平。结果:过表达ATP5J可上调线粒体膜电位水平和线粒体ATP荧光强度、诱导细胞骨架重塑、促进细胞侵袭和TOMM20蛋白表达,抑制ROS生成(P<0.01)。相反,敲减ATP5J显著降低线粒体膜电位和线粒体ATP荧光强度,显著降低细胞侵袭能力和TOMM20表达,促进ROS产生,阻断细胞骨架重塑(P<0.01)。结论:ATP5J可调节肝癌细胞线粒体能量转化,其通过TOMM20调节线粒体膜电位水平和线粒体ATP产生介导细胞骨架重塑影响肝癌细胞转移。

Residual Stress and Fracture Toughness Study in A516 Gr70 Steel Joints Welded and Repaired by Arc Processes

Structural components made of steel are used in several areas and require welding for assembly. In some situations, repair of the weld bead, also performed by electric arc welding, can be used to correct, and eliminate any discontinuities. However, electric arc welding causes the presence of residual stresses in the joint, which can impair its performance and not meet specific design requirements. In this paper, welded joints made of ASTM A 516 GR 70 steel plates, with a thickness of 30.5 mm, welded by the MAG—Metal Active Gas process (20% CO2) and using a “K” groove were analysed. The joints were manufactured with seven welding passes on each side of the groove. After welding, one batch underwent repair of the bead by TIG welding (Tungsten Insert Gas) and another batch underwent two repairs by TIG welding. Were presented results of the behaviour of the residual stress profile measured by X-ray diffraction and the Vickers microhardness profile in the joints as well the fracture toughness in the conditions only welded and submitted to repairs. The results indicated that the greater number of repair passes reduced the residual compressive stress values obtained in the material manufacturing process and caused a stabilization on the Vickers hardness values. It was concluded that compressive residual stresses did not play a major role in the R-curve results. The presence of discontinuities in the welded joint caused greater influence on the behaviour of the R curve.

Dietary Green Tea Extract and Antioxidants Improve Insulin Secretory Functions of Pancreatic β-Cells in Mild and Severe Experimental Rodent Model of Chronic Pancreatitis

Chronic pancreatitis (CP) is a progressive inflammatory disorder of the pancreas. It is predominantly idiopathic (with an unknown cause) in India and mostly due to alcohol in the West. Diabetes that occur secondary to chronic pancreatitis (T3c Diabetes) is often brittle, and is difficult to attain normoglycemia with conventional treatment requiring multiple doses of insulin. Mild and severe model of CP was induced in mice by repeated intraperitoneal injections of cerulein and L-arginine respectively with an intent to study islet dysfunction and develop therapeutic strategy in animal models of CP. Dietary intervention of epigallocatechin-3-gallate (EGCG) was tested in both the models of CP for its beneficial effects on insulin secretory functions. Pancreata collected upon euthanasia were used to study alterations in the morphology of pancreatic parenchyma and inflammation by staining with H&E and fibrotic changes by Masson’s trichrome and picrosirius staining. Insulin secretory functions of islets were evaluated to test the efficacy of the dietary intervention on β-cell functions. Intraperitoneal glucose tolerance test was performed to monitor the glucose homeostasis before and after the dietary intervention. Both the models resulted in CP with dispersed acini, inflammation and fibrosis. The loss of acini and extent of fibrosis was more in L-arginine model. 2-fold improvement in glucose-stimulated insulin secretory functions of islets was observed with 0.5% EGCG dietary intervention in cerulein model of CP and 1.6-fold in L-arginine model of CP. A further improvement in insulin secretion by 3.2-fold was observed with additional dietary supplements like N-acetyl cysteine, curcumin in combination with EGCG. Our results thus demonstrate and highlight the therapeutic potential of dietary green tea (EGCG) supplementation in reversing islet dysfunction and improving glucose homeostasis in experimental chronic pancreatitis in mice.

一种基于Davinci和Neo4j图数据库的微博水军快速识别方法研究

研究探讨了大语言模型和图数据库技术在微博水军识别领域的应用。提出了一种基于Davinci模型的微博水军快速识别方法,并利用提示词工程识别水军账号概率及线索,同时运用Neo4j图数据库实施社群分析,绘制热点事件水军传播网络,识别水军团伙。结果表明,该方法能较好地识别微博水军账号,且速度快,结合人工研判效果明显。探讨了大语言模型在水军识别中的应用前景,研究分析了水军识别过程中基于标签数据集的模型微调、提示词构建、用户社群分析等关键技术。为相关部门提供了一种利用大语言模型工具支持分析工作的新思路,对未来水军监测工作具有一定参考价值。

OPTIMAL ERROR ESTIMATE IN L~∞(J;L^2(Ω)) NORM OF FEM FOR A MODEL FOR COMPRESSIBLE MISCIBLE DISPLACEMENT IN POROUS MEDIA

We’ll study the FEM for a model for compressible miscible displacement in porous media which includes molecular diffusion and mechanical dispersion in one-dimensional space.A class of vertices-edges-elements interpolation operator ink is introduced.With the help of ink(not elliptic projection),the optimal error estimate in L∞(J;L2(Ω)) norm of FEM is proved.

毒素清对肺炎痰热证大鼠肺组织Janus激酶/信号转导和转录激活因子信号通路的影响

目的 观察肺炎痰热证大鼠肺组织Janus激酶/信号转导和转录激活因子(JAK/STAT)转导通路,探讨毒素清治疗肺炎痰热证的作用机制.方法 将Wistar大鼠按随机数字表法分为正常组、肺炎组、肺炎痰热证组、阳性对照组、毒素清组,每组12只.制备细菌性肺炎痰热证大鼠模型,采用免疫组化法测定肺组织p-JAK2、p-STAT1、p-STAT3、细胞信号转导抑制蛋白3(SOCS3)表达,采用逆转录-聚合酶链反应(RT-PCR)测定肺组织SOCS3 mRNA表达.结果 与正常组比较,肺炎组、肺炎痰热证组肺组织p-JAK2、p-STAT1、p-STAT3、SOCS3蛋白及SOCS3 mRNA表达均显著升高(均P【0.01),且肺炎痰热证组较肺炎组升高明显(均P【0.05).与肺炎痰热证组相比,毒素清组、阳性对照组肺组织p-JAK2、p-STAT1、p-STAT3蛋白表达明显减弱(均P【0.01),SOCS3蛋白及mRNA表达明显增强(均P【0.01),其中,毒素清组肺组织p-JAK2、p-STAT1、p-STAT3蛋白表达较阳性对照组减弱尤为明显(均P【0.05).结论 JAK/STAT信号转导通路参与了肺炎痰热证肺组织的病理损伤过程;毒素清治疗肺炎痰热证的作用机制可能与上调SOCS3,阻断JAK/STAT信号通路,继而减少炎症因子的释放有关.