目的:观察辛伐他汀对兔腹主动脉支架植入术后平滑肌增殖和和血管平滑肌(V SM C)中α—平滑肌肌动蛋白、P 27k ip1蛋白、增殖细胞核抗原PCNA表达量的变化。方法:45只健康的新西兰雄性大白兔随机分为正常组(n=15),给予正常饮食;对照组(n=...目的:观察辛伐他汀对兔腹主动脉支架植入术后平滑肌增殖和和血管平滑肌(V SM C)中α—平滑肌肌动蛋白、P 27k ip1蛋白、增殖细胞核抗原PCNA表达量的变化。方法:45只健康的新西兰雄性大白兔随机分为正常组(n=15),给予正常饮食;对照组(n=15)和实验组(n=15),采用含1.5%胆固醇的高脂饮食加腹主动脉内皮剥脱术制造兔腹主动脉狭窄模型。内皮剥脱术后第10周实验组和对照组服用阿司匹林25 m g/d,氯吡格雷12.5 m g/d,3 d后,分别行支架植入术。术后实验组继续服用辛伐他汀5 m g/d,服药至第30 d处死动物,取腹主动脉含支架段血管,进行血管组织形态学变化观察和检测P 27k ip1蛋白、PCNA、α-平滑肌肌动蛋白在各组的表达量的变化。结果:血管造影发现内皮剥脱术后第10周实验组和对照组腹主动脉均可见有不同程度的粥样硬化斑块和血管内狭窄(P>0.05)。组织形态学观察发现服用辛伐他汀的实验组兔腹主动脉支架段内的血管膜厚度(P<0.01)、新生内膜面积(P<0.01)均明显降低,而残余管腔面积明显增大(P<0.01),且血管的狭窄程度较轻。W estern b lot结果显示:对照组腹主动脉α-平滑肌肌动蛋白的表达明显比正常组下降55.4%,辛伐他汀治疗组α-平滑肌肌动蛋白的表达比对照组进一步降低了约29.7%(P<0.05)。实验组兔腹主动脉支架段内的血管新生内膜中的V SM C的胞核P 27k ip1蛋白表达量明显升高(P<0.01),而血管新生内膜中V SM C的胞核PCNA表达量明显降低(P<0.01)。结论:辛伐他汀能明显抑制支架植入术后平滑肌细胞增殖,降低α-平滑肌肌动蛋白的表达,其机理可能与抑制平滑肌细胞DNA增殖周期的P 27k ip1蛋白表达量增加和促进DNA增殖标志物PCNA表达量降低有关。展开更多
Ectopic hamartomatous thymoma(EHT )is a rare benign tumor. We present a case of EHT, which was seen as subcutaneous mass on the left supraclavicular area in a 19-year-old man. The tumor consisted of spindle cells, epi...Ectopic hamartomatous thymoma(EHT )is a rare benign tumor. We present a case of EHT, which was seen as subcutaneous mass on the left supraclavicular area in a 19-year-old man. The tumor consisted of spindle cells, epithelial cells, adipose cells, and a small amount of lymphocytes, as described previously. Immunohistochemically, spindle cells were positive for keratin, a-smooth muscle actin, CD34 and vimentin, but negative for desmin and S- 100 protein. Lymphocytes were positive for CD45RO but negative for CD20, CD1a, and CD99. Approximately, 5% of cells were positive for MIB-1 and no cells stained for p53 and bcl-2. Recognition of EHT is important and needs to be differentiated from high-grade sarcomas such as synovial sarcoma or glandular malignant peripheral nerve sheath tumor.展开更多
文摘目的:观察辛伐他汀对兔腹主动脉支架植入术后平滑肌增殖和和血管平滑肌(V SM C)中α—平滑肌肌动蛋白、P 27k ip1蛋白、增殖细胞核抗原PCNA表达量的变化。方法:45只健康的新西兰雄性大白兔随机分为正常组(n=15),给予正常饮食;对照组(n=15)和实验组(n=15),采用含1.5%胆固醇的高脂饮食加腹主动脉内皮剥脱术制造兔腹主动脉狭窄模型。内皮剥脱术后第10周实验组和对照组服用阿司匹林25 m g/d,氯吡格雷12.5 m g/d,3 d后,分别行支架植入术。术后实验组继续服用辛伐他汀5 m g/d,服药至第30 d处死动物,取腹主动脉含支架段血管,进行血管组织形态学变化观察和检测P 27k ip1蛋白、PCNA、α-平滑肌肌动蛋白在各组的表达量的变化。结果:血管造影发现内皮剥脱术后第10周实验组和对照组腹主动脉均可见有不同程度的粥样硬化斑块和血管内狭窄(P>0.05)。组织形态学观察发现服用辛伐他汀的实验组兔腹主动脉支架段内的血管膜厚度(P<0.01)、新生内膜面积(P<0.01)均明显降低,而残余管腔面积明显增大(P<0.01),且血管的狭窄程度较轻。W estern b lot结果显示:对照组腹主动脉α-平滑肌肌动蛋白的表达明显比正常组下降55.4%,辛伐他汀治疗组α-平滑肌肌动蛋白的表达比对照组进一步降低了约29.7%(P<0.05)。实验组兔腹主动脉支架段内的血管新生内膜中的V SM C的胞核P 27k ip1蛋白表达量明显升高(P<0.01),而血管新生内膜中V SM C的胞核PCNA表达量明显降低(P<0.01)。结论:辛伐他汀能明显抑制支架植入术后平滑肌细胞增殖,降低α-平滑肌肌动蛋白的表达,其机理可能与抑制平滑肌细胞DNA增殖周期的P 27k ip1蛋白表达量增加和促进DNA增殖标志物PCNA表达量降低有关。
文摘Ectopic hamartomatous thymoma(EHT )is a rare benign tumor. We present a case of EHT, which was seen as subcutaneous mass on the left supraclavicular area in a 19-year-old man. The tumor consisted of spindle cells, epithelial cells, adipose cells, and a small amount of lymphocytes, as described previously. Immunohistochemically, spindle cells were positive for keratin, a-smooth muscle actin, CD34 and vimentin, but negative for desmin and S- 100 protein. Lymphocytes were positive for CD45RO but negative for CD20, CD1a, and CD99. Approximately, 5% of cells were positive for MIB-1 and no cells stained for p53 and bcl-2. Recognition of EHT is important and needs to be differentiated from high-grade sarcomas such as synovial sarcoma or glandular malignant peripheral nerve sheath tumor.
