The property of major cellulases from the guts of Anoplophora glabripennis larvae have been characterized. The optimal temperatures of both β 1,4 glucosidase (β glucosidase) and endo β 1,4 endoglucanase (...The property of major cellulases from the guts of Anoplophora glabripennis larvae have been characterized. The optimal temperatures of both β 1,4 glucosidase (β glucosidase) and endo β 1,4 endoglucanase (endoglucanase, Cx) are 40℃. The β glucosidase was optimally active at pH 4\^8, while the optimal activity of the endoglucanase occurred at pH 4 4 5 6 The endoglucanase was active with a wide range of pH and temperature, the levels of activity from 25℃ to 50℃ were more than 80%, and the activity remained 60% between pH 3 2 and pH 7 2. The endoglucanase exhibited higher thermal stability than β glucosidase. Both enzymes lose their activities by heat treatment at 60℃. Two isozymes of endoglucanase were detected in sodium carboxymethylcellulose polyacrymide gels (CMC gel) by chemical colorization, and purified by elution from the gel slices. The molecular weights of the two isozymes were estimated as 26kD and 39kD respectively. Moreover molecular characteristics of the two isozymes are currently underway.展开更多
A new pathogen Nosema glabripennis Zhang parasiting the longhorned beetle Anoplophora glabripennis. According to the field investigation, about 2.8% of the real pathogens for naturally dead longhorned beetles were mic...A new pathogen Nosema glabripennis Zhang parasiting the longhorned beetle Anoplophora glabripennis. According to the field investigation, about 2.8% of the real pathogens for naturally dead longhorned beetles were microsporidiums and the other pathogens were mostly saprophytic or other parasitic organisms, which parasitized the beetles after infection by the microsporidium. The results of bioassey showed that the mortality of the longhorned beetle larvae was 27.4%. The microsporidium possesses the ability of vertical dissemination and was hopeful to be an effective natural organism to control the beetles.展开更多
文摘The property of major cellulases from the guts of Anoplophora glabripennis larvae have been characterized. The optimal temperatures of both β 1,4 glucosidase (β glucosidase) and endo β 1,4 endoglucanase (endoglucanase, Cx) are 40℃. The β glucosidase was optimally active at pH 4\^8, while the optimal activity of the endoglucanase occurred at pH 4 4 5 6 The endoglucanase was active with a wide range of pH and temperature, the levels of activity from 25℃ to 50℃ were more than 80%, and the activity remained 60% between pH 3 2 and pH 7 2. The endoglucanase exhibited higher thermal stability than β glucosidase. Both enzymes lose their activities by heat treatment at 60℃. Two isozymes of endoglucanase were detected in sodium carboxymethylcellulose polyacrymide gels (CMC gel) by chemical colorization, and purified by elution from the gel slices. The molecular weights of the two isozymes were estimated as 26kD and 39kD respectively. Moreover molecular characteristics of the two isozymes are currently underway.
文摘A new pathogen Nosema glabripennis Zhang parasiting the longhorned beetle Anoplophora glabripennis. According to the field investigation, about 2.8% of the real pathogens for naturally dead longhorned beetles were microsporidiums and the other pathogens were mostly saprophytic or other parasitic organisms, which parasitized the beetles after infection by the microsporidium. The results of bioassey showed that the mortality of the longhorned beetle larvae was 27.4%. The microsporidium possesses the ability of vertical dissemination and was hopeful to be an effective natural organism to control the beetles.
