AIM:To investigate the effect of zinc finger protein A20 on chronic liver allograft dysfunction in rats.METHODS:Allogeneic liver transplantation from DA rats to Lewis rats was performed.Chronic liver allograft dysfunc...AIM:To investigate the effect of zinc finger protein A20 on chronic liver allograft dysfunction in rats.METHODS:Allogeneic liver transplantation from DA rats to Lewis rats was performed.Chronic liver allograft dysfunction was induced in the rats by administering low-dose tacrolimus at postoperative day (POD) 5.Hepatic overexpression of A20 was achieved by recombinant adenovirus (rAd.)-mediated gene transfer administered intravenously every 10 d starting from POD 10.The recipient rats were injected with physiological saline,rAdEasy-A20 (1 × 109 pfu/30 g weight) or rAdEasy (1 × 109 pfu/30 g weight) every 10 d through the tail vein for 3 mo starting from POD 10.Liver tissue samples were harvested on POD 30 and POD 60.RESULTS:Liver-transplanted rats treated with only tacrolimus showed chronic allograft dysfunction with severe hepatic fibrosis.A20 overexpression ameliorated the effects on liver function,attenuated liver allograft fibrosis and prolonged the survival of the recipient rats.Treatment with A20 suppressed hepatic protein production of tumor growth factor (TGF)1,interleukin1 ,caspase-8,CD40,CD40L,intercellular adhesion molecule-1,vascular cell adhesion molecule-1 and E-selectin.A20 treatment suppressed liver cell apoptosis and inhibited nuclear factorB activation of Kupffer cells (KCs),liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs),and it subsequently decreased cytokine mRNA expression in KCs and LSECs and reduced the production of TGF1 in HSCs.CONCLUSION:A20 might prevent chronic liver allograft dysfunction by re-establishing functional homeostasis of KCs,LSECs and HSCs.展开更多
目的探讨锌指蛋白A20(zinc finger protein A20)对脂多糖(LPS)诱导的大鼠腹膜间皮细胞(RPMCs)炎症效应的影响及可能机制。方法分离及培养RPMCs,常规传代及鉴定。取第2代细胞用于实验研究。将细胞随机分成对照组、LPS组、转染A20组、空...目的探讨锌指蛋白A20(zinc finger protein A20)对脂多糖(LPS)诱导的大鼠腹膜间皮细胞(RPMCs)炎症效应的影响及可能机制。方法分离及培养RPMCs,常规传代及鉴定。取第2代细胞用于实验研究。将细胞随机分成对照组、LPS组、转染A20组、空载体组。脂质体转染A20质粒(pGEM-Teasy-A20)至RPMCs12h后分别在LPS刺激不同时间点收获细胞提取蛋白及细胞上清液。用Western blotting检测细胞TLR4、p-IκBα、IκBα蛋白的表达;用ELISA法检测培养上清液IL-18蛋白水平。结果 LPS刺激8h后,转染A20组RPMCsTLR4蛋白表达水平无明显增高,与对照组相比,P=0.223;与LPS组及空载体组相比,差异有显著性(P=0.003,0.002)。LPS刺激1h后,转染A20组RPMCsp-IκBα蛋白无明显降解,p-IκBα/IκBα比值与对照组相比,P=0.553。与LPS组及空载体组相比,差异有显著性(P=0.001,0.001)。在LPS刺激12h后,转染A20组RPMCsIL-18蛋白分泌水平(479.12±85.79)pg/ml高于对照组(274.34±47.21)pg/ml(P=0.012),但明显低于LPS组(1049.45±185.01)pg/ml及空载体组(1028.77±192.90)pg/ml(P=0.011,0.015)。结论 A20通过对LPS信号通路中多个相关功能蛋白的负性调控作用,阻抑LPS诱导的RPMCs炎症效应。展开更多
基金Supported by The National Natural Science Foundation of China,No.30872529the PhD Program Fund of the Ministry of Education of China,No.20030610078the Chinese Postdoctoral Science Foundation,No.2003033531
文摘AIM:To investigate the effect of zinc finger protein A20 on chronic liver allograft dysfunction in rats.METHODS:Allogeneic liver transplantation from DA rats to Lewis rats was performed.Chronic liver allograft dysfunction was induced in the rats by administering low-dose tacrolimus at postoperative day (POD) 5.Hepatic overexpression of A20 was achieved by recombinant adenovirus (rAd.)-mediated gene transfer administered intravenously every 10 d starting from POD 10.The recipient rats were injected with physiological saline,rAdEasy-A20 (1 × 109 pfu/30 g weight) or rAdEasy (1 × 109 pfu/30 g weight) every 10 d through the tail vein for 3 mo starting from POD 10.Liver tissue samples were harvested on POD 30 and POD 60.RESULTS:Liver-transplanted rats treated with only tacrolimus showed chronic allograft dysfunction with severe hepatic fibrosis.A20 overexpression ameliorated the effects on liver function,attenuated liver allograft fibrosis and prolonged the survival of the recipient rats.Treatment with A20 suppressed hepatic protein production of tumor growth factor (TGF)1,interleukin1 ,caspase-8,CD40,CD40L,intercellular adhesion molecule-1,vascular cell adhesion molecule-1 and E-selectin.A20 treatment suppressed liver cell apoptosis and inhibited nuclear factorB activation of Kupffer cells (KCs),liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs),and it subsequently decreased cytokine mRNA expression in KCs and LSECs and reduced the production of TGF1 in HSCs.CONCLUSION:A20 might prevent chronic liver allograft dysfunction by re-establishing functional homeostasis of KCs,LSECs and HSCs.
