Adipose-derived stromal cells （ASCs） have gained great attention in regenerative medicine. Progress in our understanding of adult neovascularization further suggests the potential of ASCs in promoting vascular regen...Adipose-derived stromal cells （ASCs） have gained great attention in regenerative medicine. Progress in our understanding of adult neovascularization further suggests the potential of ASCs in promoting vascular regeneration, although the specific cues that stimulate their angiogenic behavior remain controversial In this study, we established a three-dimensional （3D） angiogenesis model by co-culturing ASCs and endothelial cells （ECs） in collagen gel and found that ASC-EC-instructed angiogenesis was regulated by the canonical Wnt pathway. Furthermore, the angiogenesis that occurred in implants collected after injections of our collagen gel- based 3D angiogenesis model into nude mice was confirmed to be functional and also regulated by the canonical Wnt pathway. Wnt regulation of angiogenesis involving changes in vessel length, vessel density, vessel sprout, and connection numbers occurred in our system. Wnt signaling was then shown to regulate ASC- mediated paracrine signaling during angiogenesis through the nuclear translocation of β-catenin after its cytoplasmic accumulation in both ASCs and ECs. This translocation enhanced the expression of nuclear cofactor Lef-1 and cyclin D1 and activated the angiogenic transcription of vascular endothelial growth factor A （VEGFA）, basic fibroblast growth factor （bFGF）, and insulin-like growth factor 1 （IGF-1）. The angiogenesis process in the 3D collagen model appeared to follow canonical Wnt signaling, and this model can help us understand the importance of the canonical Wnt pathway in the use of ASCs in vascular regeneration.展开更多
[目的]基于MOD17A3数据研究陕北地区植被净初级生产力(NPP)。[方法]基于利用2000—2010年MOD17A3数据集的年均NPP数据和GIS技术定量分析了陕北植被NPP的时空变化特征。[结果]榆林市东南部及延安市中北部年平均NPP大部在151~250 g C/(m^2...[目的]基于MOD17A3数据研究陕北地区植被净初级生产力(NPP)。[方法]基于利用2000—2010年MOD17A3数据集的年均NPP数据和GIS技术定量分析了陕北植被NPP的时空变化特征。[结果]榆林市东南部及延安市中北部年平均NPP大部在151~250 g C/(m^2·a);延安市中南部年平均NPP大部在300 g C/(m^2·a)以上。延安、榆林市年NPP增加的面积都在90%以上,分别为92.9%、97.8%。2000与2010年的平均NPP相比,大部分地区是增加的,只有少部分地区是减少的。增加幅度在0~50 g C/(m^2·a)的地区为榆林市长城沿线一带,增加幅度在51~90 g C/(m^2·a)的地区为榆林市大部及延安市西北部,增加幅度在91~120 g C/(m^2·a)及>120 g C/(m^2·a)以上的主要分布在榆林市东南部及延安市中部;NPP降低的区域主要分布在延安市南部的桥山林区和黄龙林区。[结论]通过实施退耕还林等生态建设工程,陕北地区植被状况得到较好的改善。展开更多
Treatment of bis（2-dimethylaminoethyl） ether（BDMAE） with nickel acetate afforded a novel 3D nickel（Ⅱ） complex [Ni（BDMAE）（H2O）3·（CH3COO）2·（H2O）2] under solvothermal conditions. Its crystal stru...Treatment of bis（2-dimethylaminoethyl） ether（BDMAE） with nickel acetate afforded a novel 3D nickel（Ⅱ） complex [Ni（BDMAE）（H2O）3·（CH3COO）2·（H2O）2] under solvothermal conditions. Its crystal structure was characterized by elemental analysis, IR spectrum, PXRD and single-crystal X-ray diffraction analysis. The complex belongs to the orthorhombic system, space group C2221 with a=8.823（2）, b=13.932（3）, c=17.563（4） , V=2158.9（8） 3, Z=4, C（12）H（36）N2NiO（10）, Mr=427.14, Dc=1.314 g/cm3, F（000）=920 and μ=0.944 mm（-1）. Single-crystal X-ray diffraction reveals that the mononuclear nickel（Ⅱ） ion is six-coordinated to one oxygen, two nitrogen atoms of the BDMAE ligand and three oxygen atoms of coordinated water molecules. The complex exhibits a 3D supramolecular structure through a variety of intermolecular and intramolecular hydrogen bonding interactions. In addition, the complex has been investigated for catalytic properties towards the Henry reaction of nitromethane with p-nitrobenzaldehyde, and the results indicated that the 1-p-nitrophenyl-2-nitroethanol product was obtained in excellent yield under optimum conditions with the complex as the catalyst.展开更多
Objective To study the effect of using improved 2D computer-assisted fluoroscopic navigation through simulating 3D vertebrae image to guide pedicle screw internal fixation.Methods Posterior pedicle screw internal fixa...Objective To study the effect of using improved 2D computer-assisted fluoroscopic navigation through simulating 3D vertebrae image to guide pedicle screw internal fixation.Methods Posterior pedicle screw internal fixation,distraction展开更多
基金funded by the National Natural Science Foundation of China(81771125,81471803,81671031)the Sichuan Province Youth Science and Technology Innovation Team(2014TD0001)
文摘Adipose-derived stromal cells （ASCs） have gained great attention in regenerative medicine. Progress in our understanding of adult neovascularization further suggests the potential of ASCs in promoting vascular regeneration, although the specific cues that stimulate their angiogenic behavior remain controversial In this study, we established a three-dimensional （3D） angiogenesis model by co-culturing ASCs and endothelial cells （ECs） in collagen gel and found that ASC-EC-instructed angiogenesis was regulated by the canonical Wnt pathway. Furthermore, the angiogenesis that occurred in implants collected after injections of our collagen gel- based 3D angiogenesis model into nude mice was confirmed to be functional and also regulated by the canonical Wnt pathway. Wnt regulation of angiogenesis involving changes in vessel length, vessel density, vessel sprout, and connection numbers occurred in our system. Wnt signaling was then shown to regulate ASC- mediated paracrine signaling during angiogenesis through the nuclear translocation of β-catenin after its cytoplasmic accumulation in both ASCs and ECs. This translocation enhanced the expression of nuclear cofactor Lef-1 and cyclin D1 and activated the angiogenic transcription of vascular endothelial growth factor A （VEGFA）, basic fibroblast growth factor （bFGF）, and insulin-like growth factor 1 （IGF-1）. The angiogenesis process in the 3D collagen model appeared to follow canonical Wnt signaling, and this model can help us understand the importance of the canonical Wnt pathway in the use of ASCs in vascular regeneration.
文摘[目的]基于MOD17A3数据研究陕北地区植被净初级生产力(NPP)。[方法]基于利用2000—2010年MOD17A3数据集的年均NPP数据和GIS技术定量分析了陕北植被NPP的时空变化特征。[结果]榆林市东南部及延安市中北部年平均NPP大部在151~250 g C/(m^2·a);延安市中南部年平均NPP大部在300 g C/(m^2·a)以上。延安、榆林市年NPP增加的面积都在90%以上,分别为92.9%、97.8%。2000与2010年的平均NPP相比,大部分地区是增加的,只有少部分地区是减少的。增加幅度在0~50 g C/(m^2·a)的地区为榆林市长城沿线一带,增加幅度在51~90 g C/(m^2·a)的地区为榆林市大部及延安市西北部,增加幅度在91~120 g C/(m^2·a)及>120 g C/(m^2·a)以上的主要分布在榆林市东南部及延安市中部;NPP降低的区域主要分布在延安市南部的桥山林区和黄龙林区。[结论]通过实施退耕还林等生态建设工程,陕北地区植被状况得到较好的改善。
基金supported by the National Natural Science Foundation of China(No.21271136)the Key Science Foundation of Anhui Provincial Education Department(No.KJ2016A772)the Research Team of Anhui Provincial Education Department(Photoelectric Information Materials and New Energy Devices)
文摘Treatment of bis（2-dimethylaminoethyl） ether（BDMAE） with nickel acetate afforded a novel 3D nickel（Ⅱ） complex [Ni（BDMAE）（H2O）3·（CH3COO）2·（H2O）2] under solvothermal conditions. Its crystal structure was characterized by elemental analysis, IR spectrum, PXRD and single-crystal X-ray diffraction analysis. The complex belongs to the orthorhombic system, space group C2221 with a=8.823（2）, b=13.932（3）, c=17.563（4） , V=2158.9（8） 3, Z=4, C（12）H（36）N2NiO（10）, Mr=427.14, Dc=1.314 g/cm3, F（000）=920 and μ=0.944 mm（-1）. Single-crystal X-ray diffraction reveals that the mononuclear nickel（Ⅱ） ion is six-coordinated to one oxygen, two nitrogen atoms of the BDMAE ligand and three oxygen atoms of coordinated water molecules. The complex exhibits a 3D supramolecular structure through a variety of intermolecular and intramolecular hydrogen bonding interactions. In addition, the complex has been investigated for catalytic properties towards the Henry reaction of nitromethane with p-nitrobenzaldehyde, and the results indicated that the 1-p-nitrophenyl-2-nitroethanol product was obtained in excellent yield under optimum conditions with the complex as the catalyst.
文摘Objective To study the effect of using improved 2D computer-assisted fluoroscopic navigation through simulating 3D vertebrae image to guide pedicle screw internal fixation.Methods Posterior pedicle screw internal fixation,distraction
