对圆孢芽孢杆菌（Bacillus globisporus）A95产生的抗菌蛋白进行了分离纯化，并研究了其部分性质。菌株摇瓶发酵液离心后的上清液经硫酸铵40％～60％分级盐析获得粗提物。粗提物溶液经Sephadex G-100柱层析、DEAE-Sepharose Fast Flow...对圆孢芽孢杆菌（Bacillus globisporus）A95产生的抗菌蛋白进行了分离纯化，并研究了其部分性质。菌株摇瓶发酵液离心后的上清液经硫酸铵40％～60％分级盐析获得粗提物。粗提物溶液经Sephadex G-100柱层析、DEAE-Sepharose Fast Flow离子交换层析、Sephadex G-50脱盐纯化获得的抗菌蛋白，通过性质检测、电泳及MALDL-TOF-MS质谱检测，表明为分子量1．464～1．529kD的一组环肽类抗菌蛋白。该抗菌蛋白对多种家蚕病原真菌具有较好的抗菌活性，参照美国国家临床试验标准化委员会（NCCLS）提出的标准，用微量液基稀释法分别测定了对绿僵菌、白僵菌和黄曲霉的最小抑菌浓度（MIC）和最小杀菌浓度（MFC）。该抗菌蛋白对热稳定，对胰蛋白酶、蛋白酶K、氯仿有一定的耐受性，对酸部分敏感，对碱稳定。展开更多
In order to improve the spinnabilities of A95 alumi- na silica sols, the influences of addition and polymeriza- tion degree of polyvinyl alcohol on spinnabilities of alu- mina silica sols were researched. The viscosit...In order to improve the spinnabilities of A95 alumi- na silica sols, the influences of addition and polymeriza- tion degree of polyvinyl alcohol on spinnabilities of alu- mina silica sols were researched. The viscosity and rheo- logical properties of the sols, mierostructure, infrared spectra, average diameter and diameter distribution oJ the gel fibers, BET specific surface area and distribution of pore diameters were measured. The results show that iJ there is no spinning agent, the A95 sols have no spinna- bility ; when PVA 2099 addition is in the range of l. 0% -2.0% （in mass, the same hereinafter） , the prepared sols have good spinnability, the gel fibers produced are thin and uniform, and the shot content is low ; BET spe- cific surface area and gas absorption amount of A95 polycrystalline alumina fibers are the largest when PVA 2099 addition is 2. 0% ; the gel fibers are thick and non-uniform, and the shot content is high when PVA 2099 addition is 4. 0% ; with polymerization degree oJ PVA increasing, the average diameter and diameter dis- tribution range of the gel fibers increase gradually; when using PVA 1799 and PVA 1899, respectively, the spun gel fibers are thin and uniform.展开更多
文摘In order to improve the spinnabilities of A95 alumi- na silica sols, the influences of addition and polymeriza- tion degree of polyvinyl alcohol on spinnabilities of alu- mina silica sols were researched. The viscosity and rheo- logical properties of the sols, mierostructure, infrared spectra, average diameter and diameter distribution oJ the gel fibers, BET specific surface area and distribution of pore diameters were measured. The results show that iJ there is no spinning agent, the A95 sols have no spinna- bility ; when PVA 2099 addition is in the range of l. 0% -2.0% （in mass, the same hereinafter） , the prepared sols have good spinnability, the gel fibers produced are thin and uniform, and the shot content is low ; BET spe- cific surface area and gas absorption amount of A95 polycrystalline alumina fibers are the largest when PVA 2099 addition is 2. 0% ; the gel fibers are thick and non-uniform, and the shot content is high when PVA 2099 addition is 4. 0% ; with polymerization degree oJ PVA increasing, the average diameter and diameter dis- tribution range of the gel fibers increase gradually; when using PVA 1799 and PVA 1899, respectively, the spun gel fibers are thin and uniform.
