Significant association between ABO blood group and pancreatic cancer

AIM:To evaluate whether the ABO blood group is related to pancreatic cancer risk in the general population of the United States.METHODS:Using the University of Pittsburgh's clinicalpancreatic cancer registry,the blood donor database from our local blood bank (Central Blood Bank),and the blood product recipient database from the regional transfusion service (Centralized Transfusion Service) in Pittsburgh,Pennsylvania,we identified 274 pancreatic cancer patients with previously determined serological ABO blood group information.The ABO blood group frequency was compared between these patients and 708842 individual,community-based blood donors who had made donations to Pittsburgh's Central Blood Bank between 1979 and 2009.RESULTS:The frequency of blood group A was statistically significantly higher amongst pancreatic cancer patients compared to its frequency amongst the regional blood donors [47.63% vs 39.10%,odds ratio (OR)=1.43,P=0.004].Conversely,the frequency of blood group O was significantly lower amongst pancreatic cancer patients relative to the community blood donors (32.12% vs 43.99%,OR=0.60,P=0.00007).There were limited blood group B (n=38) and AB (n=17) pancreatic cancer patients;the overall P trend value comparing patient to donor blood groups was 0.001.CONCLUSION:The ABO blood group is associated with pancreatic cancer risk.Future studies should examine the mechanism linking pancreatic cancer risk to ABO blood group.

Relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhabitants of China

AIM To study the relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhebitants of China, which is a unique Littoral high-risk area of esophageal carcinoma in China. The poor communication and transportation in the psst has made Cheoshan a relatively closed area and kept its culture and costure of old China thousend years ago.``METHODS Data on age, sex, ABO blood type and X-rayor psthological diagnose of the pstients with carcinoma of esophagus or cardia were collected from the Tumor Hospital. First Affiliated Hospital, Second Affiliated Hospital of Shantou University Medical College; and the Central Hospital of Shantou and the Central Hospital of Jieyang. A total of 6685 pstients with esophageal carcinoma (EC) and 2 955 patients with cardiac cancer (CC) in Chaoshen district were retrospectively assessed for their association with ABO blood groups.``RESULTS The distribution of ABO blood groups in patients with EC or CC was similar to the norrnal local population in Chaoshen. However, blood group B in male patients with CC and in the pstients with carcinoma in the upper third esophagus was 2.3% and 4.7% higher than the corresponding controls. The relative risk B: O was 1. 1415 (P＜0.05)and 1 .2696 (P＜0.05), respectively. No relationship was found between ABO blood groups and tumor differentiation.``CONCLTUSION ABO blood group B is associated with the incidence of CC in male individuals and carcinona in the upper third esophagus. The distribution of ABO blood groups varies in the different geographical and ethnic groups. As a result, proper controls are very important for such studies.

ABO blood group is a predictor of survival in patients with laryngeal cancer

Background: Whether the ABO blood group is associated with the survival of patients with laryngeal cancer remains unknown. The purpose of this study was to investigate the association between the ABO blood group and clinico?pathologic characteristics of patients with laryngeal cancer and assess whether the ABO blood group was associated with prognosis.Methods: We analyzed the records of 1260 patients with laryngeal cancer who underwent curative treatment at Sun Yat?sen University Cancer Center between January 1993 and December 2009. The Chi?square test was used to assess the relationship between the ABO blood group and clinicopathologic characteristics. The Kaplan–Meier method was used to estimate 3?, 5?, and 10?year overall survival(OS) rates. The Cox proportional hazards model was used in univariate and multivariate analyses of OS.Results: No signiicant association was found between the ABO blood group and clinicopathologic characteristics except for primary tumor site. The median OS for patients with blood groups A, B, AB, and O were 87.0, 80.0, 90.0, and 72.5 months, respectively. The 3?, 5?, and 10?year OS rates were 82.4%, 76.0%, and 67.5% for patients with blood group A; 77.4%, 69.8%, and 58.4% for patients with blood group B; 82.2%, 73.1%, and 65.6% for patients with blood group AB; and 71.7%, 66.4%, and 55.5% for patients with blood group O, respectively. Univariate and multivariate analyses showed that the ABO blood group had signiicant efects on prognosis in patients with laryngeal cancer.Conclusions: The ABO blood group is associated with survival in patients with laryngeal cancer. Patients with blood group O had signiicantly shorter OS than patients with other ABO blood groups.

ABO Blood Groups and Their Relationship with Coagulation Factor VIII in Healthy Adults

Background: ABO blood group distribution defers with racial and geographic variations. They are related to diseases like cardiovascular diseases, cerebral thromboembolism. ABO blood group system may influence coagulation factor VIII which may increase the future risk of thrombosis. Aim: To assess the relation of ABO blood group with coagulation factor VIII in healthy adults. Material and Methods: A prospective type of analytical cross-sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka from July 2019 to June 2020. After obtaining ethical clearance, a total of 190 healthy adults were selected from different areas of Dhaka city based on inclusion and exclusion criteria, with ages ranging from 18 - 45 years. The subjects were interviewed and detailed history regarding personal, family, medical and drug were taken. Prior to sample collection, informed written consent was taken from the participants. Individuals of blood group A were selected as group A, blood group B as group B, blood group AB as group AB and blood group O as group O. Coagulation factor VIII was measured in the Department of Hematology and BMT Unit, Dhaka Medical College Hospital, Dhaka. Blood grouping was done in the Department of Physiology, Dhaka Medical College, Dhaka. Statistical Analysis: For statistical analysis, ONE way ANOVA followed by Bonferroni test were considered using SPSS 25.0 version. Results: In this study, blood group B was most common (33.2%). Coagulation factor VIII was significantly higher (p < 0.001) in blood group A (105.76% ± 11.82%), B (112.00% ± 15.02%), AB (109.80% ± 11.93%) than blood group O (82.00% ± 12.86%). No significant difference was observed among A, B and AB blood groups regarding coagulation factor VIII. Conclusions: It can be concluded that blood group A, B, AB individuals may have more chance of thrombosis due to significantly higher coagulation factor VIII than blood group O individuals.

ABO血型次要不合的异基因造血干细胞移植合并过客淋巴细胞综合征患者的诊治

目的探讨异基因造血干细胞移植(allogeneic hematopoietic stem cell transplantation,allo-HSCT)患者发生过客淋巴细胞综合征(passenger lymphocyte syndrome,PLS)的临床特点和诊治方法。方法回顾2018年12月至2022年12月在苏州弘慈血液病医院行allo-HSCT的489名患者。分析移植后并发PLS的4名患者的临床过程、诊治措施及预后。结果489名allo-HSCT患者中,4例确诊为PLS,供受体ABO血型均为次要不合(O供A或B)。PLS在allo-HSCT中的总体发生率为0.82%(4/489),在供受体ABO血型次要不合的移植中发生率为2.2%(4/179)。PLS发生在供体干细胞回输6~13 d,临床表现为头晕乏力明显,腰痛、黄疸、尿色加深,实验室检查血红蛋白迅速下降,间接胆红素、乳酸脱氢酶升高,尿胆原阳性、直接抗人球蛋白试验(DAT)阳性,并且血浆中均检测出针对受者红细胞的抗-A或抗-B。治疗上予以输注O型洗涤红细胞,甲强龙,丙种球蛋白、利妥昔单抗等治疗后患者溶血表现改善。所有患者都获得了粒系、血小板植入,并且红细胞脱离输注时间均在3周内。结论PLS是异基因造血干细胞移植少见的并发症,主要发生在ABO血型次要不合的allo-HSCT患者中,及时处置临床预后良好。

先天性腹膜鞘状突未闭疾患与ABO血型的相关性

目的探讨先天性腹膜鞘状突未闭(PPV)疾患与ABO血型的相关性。方法回顾性分析2003年1月至2021年12月该院收治的1128例存在PPV疾患儿童(病例组)的临床资料和同期收治的4208例其他疾病儿童(对照组)的临床资料。病例组根据疾病类型分为腹股沟斜疝组(738例)、精索鞘膜积液组(228例)和隐睾组(162例)。比较各组患儿ABO血型分布情况。结果腹股沟斜疝组患儿中A、B、O、AB型血分别占28.3%(209/738)、28.5%(210/738)、28.7%(212/738)、14.5%(107/738),对照组患儿中A、B、O、AB型血分别占29.9%(1257/4208)、29.4%(1237/4208)、22.6%(949/4208)、18.2%(765/4208),2组患儿血型构成比较,差异有统计学意义(P<0.05)。A、B、AB型血儿童较O型血儿童患腹股沟斜疝风险低[优势比(OR)=0.744、0.760、0.626,95%可信区间(95%CI):0.604~0.917、0.617~0.936、0.487~0.805,P=0.006、0.010、<0.001]。调整年龄、性别、民族等影响因素后O型血儿童患腹股沟斜疝的风险较非O型血高(OR=1.396,95%CI:1.160~1.679,P<0.001),AB型血儿童患腹股沟斜疝的风险较非AB型血低(OR=0.742,95%CI:0.590~0.934,P=0.011)。结论该院所诊治的儿童病例中因先天性PPV发生腹股沟斜疝与ABO血型可能存在相关性,ABO血型为小儿腹股沟斜疝发生的一个影响因素。

ABO血型对健康年轻国人周围神经传导的影响

目的 探讨ABO血型对周围神经传导产生的影响,为周围神经病的预防及治疗提出新思路和方法。方法 选择延边大学医学院在校健康青年志愿者856名,检测所有研究对象ABO血型,及正中、尺、腓、胫神经神经传导参数和F波,分析不同血型间的神经传导差异。结果 与左侧相比,正中神经O型传导机能右侧降低,尺神经B、O型波幅右侧升高,非B型腓神经传导速度右侧升高,胫神经O型远位潜伏期/波幅(基-峰)右侧延长/升高(P均<0.05)。正中和胫神经传导机能AB型优于非AB型;腓神经传导机能非O型优于O型;各神经F波最短潜伏期O型较非O型延长(P均<0.05)。超过可信区上限或低于下限的比率:正中和尺神经AB型小于非AB型,腓和胫神经A型小于非A型(P均<0.05)。神经传导机能显著低下神经数尺、腓、胫神经中O型显著。结论 神经传导机能AB型优于非AB型;神经传导低下比率O型明显,左侧与右侧传导差异O型最明显。

Serum anti‑blood type IgG/IgM binding and cytotoxicity to pig PBMC and RBC

Objective: TO investigate the killing differences of human blood group antibodies IgG and IgM on PBMC and RBC in peripheral blood of genetically modified pigs, and provide theoretical basis for the selection of pig blood group for clinical application of porcine xenotransplantation. Methods: Serum samples were collected from 20 healthy subjects, 20 patients with end‑stage renal disease and 20 brain dead organ donors, and divided into 4 groups on the basis of ABO blood group (A: n=20;B: n=17;AB: n=7;O: n=16). Flow cytometry was used to detect antibody binding or complement dependent cytotoxicity test (CDC) between human serum and O blood group Wild‑type (WT), α1, 3‑galactosyltransferase gene‑knockout(GTKO), cytidine monophosphate‑N‑acetylneuraminic acid hydroxylase gene‑knockout (GTKO/β4GalNT2KO), β‑1, 4N‑acetylgalactosaminyltransferase gene‑knockout(GTKO/CMAHKO) and mononuclear cells (PBMC) and red blood cells (RBC) of Triple knockout (TKO/hCD55) porcine peripheral blood, respectively. Results: There was no significant difference in binding and killing of human serum antibodies with blood group A, B, AB and O on PBMC of WT pigs, GTKO pigs, GTKO/β4GalNT2KO pigs, GTKO/CMAHKO pigs and TKO/hCD55 pigs, respectively. There was no significant difference in RBC binding with RBC of WT pig, GTKO pig, GTKO/β4GalNT2KO pig, GTKO/CMAHKO pig and TKO/hCD55 pig. Conclusion: The selection of recipients of pigs with type O blood group can be done without considering blood group.

肠道菌群与人类ABO血型

肠道菌群因与人类健康和疾病密切相关而成为当前生命科学和医学领域的研究热点。近年来,各国多个权威实验室先后报道了肠道菌群与人类ABO血型之间的相互作用。本文首先将对人类肠道菌群的概念、组成、结构及生理功能进行简要介绍,分析肠道菌群相关研究的趋势。其次,将基于现有的研究成果对肠道菌群与人类血型之间的关系进行简要论述,既包含肠道菌群对于人类血型的改变,也包括人类血型对于肠道菌群组成结构的影响。最后,分析当前关于肠道菌群与人类血型的研究成果如何更好地应用于输血科研和输血治疗领域。

ABO疑难血型的原因及鉴定方法分析

目的分析ABO疑难血型的原因及鉴定方法,确保临床输血安全。方法应用ABO疑难血型三步分析法,对本院2020年6月至2022年12月本院接收的110例ABO正反定型不一致标本进行分析鉴定。结果110例ABO正反定型不一致标本中,人为因素造成的有18例(16.36%);由于疾病本身或治疗因素造成的有92例(83.64%)。人为因素中,人员技术问题占比27.78%、未按规程操作占比22.22%、标本污染占比22.22%、标本标签错误占比11.11%、离心机问题占比11.11%、试剂过期占比5.56%。92份因疾病本身或治疗因素中,抗原因素28例,占比30.43%;抗体因素64例,占比67.57%。结论应用ABO疑难血型三步分析法能快速有效的解决疑难血型的鉴定,确保临床输血安全。

一例ABO血型A抗原减弱的血清学及其基因变异分析

目的分析1例A抗原表达减弱患者的血清学及分子生物学特点,并探讨其分子生物学机制及输血策略。方法应用常规血清学方法进行ABO血型鉴定,测序分型确定ABO基因型。结果血清学格局为A抗原减弱,基因测序表明外显子6存在c.261delG杂合突变、外显子7存在c.467C>T杂合突变、内含子6存在c.374+5G>A杂合突变,患者基因型为A102/AEL04/O01,表型为Ael。结论A等位基因内含子6的c.374+5G>A突变,导致转录过程形中成不同剪接变异体,造成A型糖基转移酶的活性减弱或失活,是本例患者A抗原减弱的主要原因。

ABO血型不合对异基因造血干细胞移植的影响

目的:分析ABO血型不合对异基因造血干细胞移植(allo HSCT)的影响。方法: 对北京大学人民医院血液病研究所 148例ABO血型不合的allo HSCT受者按不同的移植方式进行分析,并选用同期 85例ABO血型相合的受者作比较。结果:主要ABO血型不合骨髓移植(BMT)组红细胞输注量多,红系恢复时间长。ABO血型不合的BMT、外周血干细胞移植(PBSCT)以及BMT+PBSCT患者急性移植物抗宿主病 (aGVHD)的发生率、巨细胞病毒(CMV)发生率及 5年无病存活率与ABO血型相合患者相比差异无统计学意义。结论:ABO血型不合不影响造血干细胞移植的植活、主要合并症及预后。ABO血型不合的PBSCT与BMT相比,红细胞输注量少,红系恢复较快。

ABO血型与胃癌发生风险的病例-对照研究及meta分析

目的通过病例一对照研究及meta分析探讨ABO血型系统与胃癌发生风险的关系。方法收集第二军医大学长海医院930例胃癌患者及8426例非胃癌患者进行病例一对照研究；检索PubMed数据库，检索起始时间不限，截止时间为2013年12月31日。将笔者所做病例一对照研究一并纳入分析，由2名研究者独立筛取文献并提取数据，采用纽卡斯尔-渥太华量表（NOS）进行文献质量评价，采用Meta—Analyst3．13软件进行meta分析。结果病例对照研究提示：血型A是胃癌发生的可能危险因素（OR=1．58，95％CI为1．37～1．81）；meta分析结果显示：血型A较非A血型人群胃癌发生风险有所增加（0R=1．28，95％CI为1．01～1．62），血型O发生胃癌风险低于非O血型人群（OR=0．84，95％CI为0．73～0．96），但亚组分析发现结果在国内外人群中存在差异。结论ABO血型系统与胃癌发生风险存在关联，血型A是胃癌发生的可能危险因素之一。

新生儿ABO溶血病的早期诊断及治疗

目的探讨新生儿ABO溶血病的早期诊断及治疗。方法回顾分析2003年4月～2006年4月收治的新生儿ABO溶血病287例。依据患儿出生后是否即刻行脐血溶血3项检查和是否静脉应用大剂量丙种球蛋白治疗分为4组：行脐血溶血3项检查者，分为常规Ⅰ组（23例）和丙种球蛋白Ⅰ组（丙球I组，92例）；未行脐血溶血3项检查者，分为常规Ⅱ组（43例）和丙球Ⅱ纽（129例）。分析4组基本状况（年龄、性别、出生体质量等），血清总胆红素（TB）、血红蛋白（Hb）水平。结果4组性别、胎龄、出生体质量、血型、黄疸出现时间、发生黄疸高峰时间、光疗持续时间、平均住院时间等均无显著性差异（Pa〉0．05）。与丙球Ⅰ组和常规Ⅰ组比较，丙球Ⅱ组和常规Ⅱ组入院日龄明显较大（P。〈0．01），TB水平明显增高（P。〈0．01）。与常规组比较，丙球组黄疸高峰值明显降低（Pa〈0．01）。人院时Hb水平，除丙球Ⅰ组偏低外（P〈0．01），余3组间均无显著性差异（Pa〉0．05）。住院1周后复查Hb，丙球Ⅰ组Hb水平降低程度较常规Ⅰ组小。结论脐血溶血3项检查对于新生儿ABO溶血病早期诊断具有重要价值；在常规治疗基础上，静脉应用大剂量丙种球蛋白必须早期（出生48h内）进行才能更为有效降低患儿胆红素水平和贫血程度。

孕妇ABO血型抗A(B)抗体效价检测分析

目的了解该地区孕妇ABO血型抗A(B)IgG抗体效价异常率,分析其在新生儿溶血病(HDN)中的意义。方法采用微柱凝胶法对4000例孕周20周以上、O型血孕妇(丈夫为非O型血)进行血清抗A(B)IgG抗体效价检测;对抗体效价大于1:64者所分娩新生儿进行HDN试验检测,分析抗体效价与HDN发病率的关系。结果 4000例孕妇中,血清抗A(B)IgG抗体效价大于1:64者有432例,异常率为10.8%(432/4000);295例血清抗A(B)IgG抗体效价大于1:64者所分娩的新生儿中,HDN发病率为53.9%(159/295)。结论随着血清IgG抗A(B)效价增高,HDN发病率也增高;产前抗体效价检测是可用于判断HDN发病风险的筛查指标。

中国56个民族ABO血型分布

目的了解中国56个民族ABO血型分布特点。方法收集中国56个民族437篇国内外发表的ABO血型资料并进行统计分析。结果除部分云南少数民族有较高的A型频率外,大多南方民族有较高的O型频率;北方民族中B型频率高者居多,O型频率高者较少。结论结果提示56个民族ABO血型分布特点与其地域分布有相关性。

83例疑难ABO血型鉴定及分析

目的检测和鉴定ABO疑难血型,为安全输血提供质量保障。方法采用血型血清学方法进行盐水介质正、反定型;对盐水介质正、反定型不合者使用聚凝胺法检测和不规则抗体检测;采用吸收试验和放散试验进行ABO血型的检测,以准确鉴定ABO疑难血型。结果 83例标本ABO血型检测结果为,AB亚型9例、B亚型1例、弱A抗原7例、弱B抗原6例、低抗-A效价2例、低抗-B效价3例、不规则抗体阳性7例、无抗体O型4例、低抗-B效价O型2例、无抗-B抗体A型5例;37例标本经盐水介质和Polybrine法检测,正、反定型结果符合。结论用聚凝胺法、吸收试验和放散试验进行ABO疑难血型检测,能准确、及时鉴定ABO疑难血型。