To characterize the patterns of ABR waves in tree shrews, we must understand the hearing sensitivity and auditory function of healthy adult tree shrews. Fifteen tree shrews(30 ears) were stimulated with clicks and ton...To characterize the patterns of ABR waves in tree shrews, we must understand the hearing sensitivity and auditory function of healthy adult tree shrews. Fifteen tree shrews(30 ears) were stimulated with clicks and tone-pips at 11 different frequencies from 1 to 60 kHz. The ABR waves were recorded and analyzed. The ABR consisted of five to seven positive waves in the first 10 ms after a click stimulus, and the average hearing threshold of component III was 27.86 ± 3.78 dB SPL. Wave III was the largest and most clear. The ABR threshold was related to the tone-pip sitmulus by a "U" shaped curve. The sensitive frequency was approximately 8 kHz in tree shrews. The latencies systematically decreased with increasing stimulus frequencies. The ABR amplitudes of wave III increased as the sound pressure level increased. All of these results provide an empirical basis for future studies of hearing diseases in tree shrews.展开更多
The auditory brainstern responses(ABR)and middle latency responses(MLR)were recorded in 24 normal mice with a 7S11A processor,and the normalparameters of ABR and MLR obtained.The results showed that the ABR of mice we...The auditory brainstern responses(ABR)and middle latency responses(MLR)were recorded in 24 normal mice with a 7S11A processor,and the normalparameters of ABR and MLR obtained.The results showed that the ABR of mice werecomposed of 6 waves,waves Ⅰ to Ⅵ.The mean threshold was 35.12±8.03 dB ,whichwas significantly different from the 22.63 dB of ABR found in guinea pigs.Thelatency of ABR waves Ⅰ to Ⅵizn the mice was found to be 1.30±8.03 ms ;2.02±0.24ms ;2.90±0.27 ms ;3.80±0.24 ms ;4.87±0.42 ms and 5.83±0.40 ms respectively.Nosex or strain differences were found.The MLR waveform of the mice were similar tothose of human and the latency was found to be P0 3.94±1.76 ms;P8.38±1.57ms;Na 14.98±2.54 ms;Pa 24.56±3.15 ms rcspectively.It is suggested that mice can beused for hearing research.Since the Na and Pa waves of MLR were unstable under anes-thesia,testing conditions for determining MLR in mice need further study.展开更多
Objective To analyze the characteristics of auditory brainstem response (ABR) in presbycusis patients el-der than 90 years. Methods Fourteen presbycusis patients elder than 90 years (presbycusis group, 91.1.4 ± 1...Objective To analyze the characteristics of auditory brainstem response (ABR) in presbycusis patients el-der than 90 years. Methods Fourteen presbycusis patients elder than 90 years (presbycusis group, 91.1.4 ± 1.3 years, 26 ears) and 9 normal-hearing young adults (control group, 22.7 ± 1.2 years, 18 ears) participated in the study. Alternative click-evoked ABRs were recorded in both groups. The peak latency (PL) of peak I,Ⅲ, and V, and the inter-peak latency (IPI) of I-Ⅲ,Ⅲ-V, and I-V were compared between groups. Results In elder presbycusis patients, the occurrence rate of peak I andⅢwere both 76.9%, and that of peak V was 84.6%. In presbycusis group, the peak latencies of I, Ⅲ, V were significantly longer than that of control group (P<0.001). There was no significant difference between groups in the IPI of peak I-IⅢ (P=0.298, peakⅢ-V (P=0.254) and peak I-V (P=0.364). Conclusions Auditory brainstem responses in presbycusis pa-tients elder than 90 years showed worse wave differentiation.展开更多
Objectives To investigate changes in evoked potentials and structure of the guinea pig cochleae during whole cochlear perfusion with glutamate. Methods CM, CAP, DPOAE, and ABR were recorded as indicators of cochlear f...Objectives To investigate changes in evoked potentials and structure of the guinea pig cochleae during whole cochlear perfusion with glutamate. Methods CM, CAP, DPOAE, and ABR were recorded as indicators of cochlear functions during whole cochlear perfusion. The morphology of the cochlea was studied via transmission electron microscopy. Results There were no significant changes in DPOAE amplitude before and after glutamate perfusion. CM I/O function remained nonlinear during perfusion. ABR latencies were delayed following glutamate perfusion. The average CAP threshold was elevated 35 dB SPL following glutamate perfusion.. The OHCs appeared normal, but the IHCs and afferent dendrites showed cytoplasmic blebs after glutamate perfusion. Conclusions While being a primary amino acid neurotransmitter at the synapses between hair cells and spiral ganglion neurons, excessive glutamate is neurotoxic and can destroy IHCs and spiral ganglion neurons. The technique used in this study can also be used to build an animal model of auditory neuropathy.展开更多
The analysis of the Auditory Brainstem Response (ABR) is of fundamental importance to the investigation of the auditory system behavior, though its interpretation has a subjective nature because of the manual process ...The analysis of the Auditory Brainstem Response (ABR) is of fundamental importance to the investigation of the auditory system behavior, though its interpretation has a subjective nature because of the manual process employed in its study and the clinical experience required for its analysis. When analyzing the ABR, clinicians are often interested in the identification of ABR signal components referred to as Jewett waves. In particular, the detection and study of the time when these waves occur (i.e., the wave latency) is a practical tool for the diagnosis of disorders affecting the auditory system. In this context, the aim of this research is to compare ABR manual/visual analysis provided by different examiners. Methods: The ABR data were collected from 10 normal-hearing subjects (5 men and 5 women, from 20 to 52 years). A total of 160 data samples were analyzed and a pair- wise comparison between four distinct examiners was executed. We carried out a statistical study aiming to identify significant differences between assessments provided by the examiners. For this, we used Linear Regression in conjunction with Bootstrap, as a method for evaluating the relation between the responses given by the examiners. Results: The analysis suggests agreement among examiners however reveals differences between assessments of the variability of the waves. We quantified the magnitude of the obtained wave latency differences and 18% of the investigated waves presented substantial differences (large and moderate) and of these 3.79% were considered not acceptable for the clinical practice. Conclusions: Our results characterize the variability of the manual analysis of ABR data and the necessity of establishing unified standards and protocols for the analysis of these data. These results may also contribute to the validation and development of automatic systems that are employed in the early diagnosis of hearing loss.展开更多
Tinnitus has often been studied using salicylate in animal models as they are capable of inducing tempo-rary hearing loss and tinnitus. Studies have recently observed enhancement of auditory evoked responses of the au...Tinnitus has often been studied using salicylate in animal models as they are capable of inducing tempo-rary hearing loss and tinnitus. Studies have recently observed enhancement of auditory evoked responses of the auditory cortex (AC) post salicylate treatment which is also shown to be related to tinnitus like behavior in rats. The aim of this study was to observe if enhancements of the AC post salicylate treatment are also present at structures in the brainstem. Four male Sprague Dawley rats with AC implanted electrodes were tested for both AC and auditory brainstem response (ABR) recordings pre and post 250 mg/kg intraperitone-al injections of salicylate. The responses were recorded as the peak to trough amplitudes of P1-N1 (AC), ABR wave V, and ABR waveⅡ. AC responses resulted in statistically significant enhancement of ampli-tude at 2 hours post salicylate with 90 dB stimuli tone bursts of 4, 8, 12, and 20 kHz. Wave V of ABR re-sponses at 90 dB resulted in a statistically significant reduction of amplitude 2 hours post salicylate and a mean decrease of amplitude of 31%for 16 kHz. WaveⅡamplitudes at 2 hours post treatment were signifi-cantly reduced for 4, 12, and 20 kHz stimuli at 90 dB SPL. Our results suggest that the enhancement chang-es of the AC related to salicylate induced tinnitus are generated superior to the level of the inferior colliculus and may originate in the AC.展开更多
目的探讨多种听力学检测方法在听性脑干反应(ABR)最大输出未引出患儿的听力学诊断中的应用价值。方法回顾性分析69例(138耳)ABR最大强度未引出患儿的临床资料,年龄42天到5岁,平均1岁6个月,鼓室导抗图均为A型或正向单峰,声反射均未引出,...目的探讨多种听力学检测方法在听性脑干反应(ABR)最大输出未引出患儿的听力学诊断中的应用价值。方法回顾性分析69例(138耳)ABR最大强度未引出患儿的临床资料,年龄42天到5岁,平均1岁6个月,鼓室导抗图均为A型或正向单峰,声反射均未引出,影像学检查内耳无畸形。69例患儿均进行ABR、耳蜗微音电位(CM)、畸变产物耳声发射(DPOAE)和听性稳态反应(ASSR)测试。结果69例138耳中,8例16耳(11.59%)记录到CM,其中10耳(7.25%)记录到DPOAE,0.5、1、2、4 kHz ASSR反应阈值分别为83.2±13.1、82.9±13.0、75.3±12.4、63.1±9.1 dB nHL,结合其他检查结果诊断为听神经病。余61例(122耳)CM和DPOAE均未引出,0.5、1、2、4 kHz的ASSR引出率分别为82.3%、81.9%、76.9%、60.2%,其中20耳ASSR各频率均未引出,102耳至少一个频率引出,0.5、1、2、4 kHz ASSR反应阈分别为93.2±6.1、99.8±7.0、105.4±5.4、108.2±9.8 dB nHL,诊断为极重度感音神经性聋。结论对于ABR最大输出强度未引出的患儿,CM和/或DPOAE引出且ASSR各频率反应阈低于感音神经性聋患儿,有助于听神经病的诊断;CM和DPOAE均未引出有助于极重度感音神经性聋的诊断,ASSR测试有助于评估其残余听力。展开更多
目的获取6月龄内婴儿的听性脑干反应（auditory brainstem response，ABR）正常值范围并分析其特点。方法2006年5月至2008年5月对听力正常、出生40天～7月龄的60例婴儿进行ABR检测，根据平均年龄分为A（6周龄）、B（3月龄）、C（6月龄...目的获取6月龄内婴儿的听性脑干反应（auditory brainstem response，ABR）正常值范围并分析其特点。方法2006年5月至2008年5月对听力正常、出生40天～7月龄的60例婴儿进行ABR检测，根据平均年龄分为A（6周龄）、B（3月龄）、C（6月龄）组，每组20例（40耳）；对照组为听力正常的成人20例（40耳），平均年龄25．2岁。对各组的ABR结果进行统计分析。结果各组100dB nHL ABR的波Ⅰ、Ⅲ、Ⅴ潜伏期的正常值范围分别为：A组：1．49±0．08、4．42±0．16、6．61±0．25ms；B组：1．47±0.07、4．35±0．20、6．50±0．25ms；C组：1．45±0．07、4．17±0．15、6．32±0．22ms；对照组：1．45±0．07、3．79±0．11、5．49±0．20ms；三组婴儿80dB nHL ABR的波Ⅰ、Ⅲ、Ⅴ潜伏期的正常值范围分别为：A组：1．63±0．08、4．52±0．17、6．74±0．26ms；B组：1．64±0．11、4．44±0．20、6．67±0．26ms；C组：1．60±0．11、4．27±0．16、6．43±0．24ms。随刺激声强度降低，ABR各波潜伏期延长。各月龄组婴儿的波Ⅲ、Ⅴ潜伏期、Ⅰ-Ⅲ、Ⅰ—V波间期均比成人延长．且随婴儿月龄增加逐渐缩短，但6月龄时仍未达到成人水平。各月龄组ABR反应阈差异无统计学意义。结论不同月龄婴儿的波Ⅰ潜伏期与成人相近；各月龄婴儿的ABR反应阈无明显差异，且与成人相近。展开更多
基金supported by Natural Science Foundation of China[grant numbers 81760188]Scientific Research Fund of Guangxi Provincial Education Department [grant numbers 2017KY0103 (02601217023C)]
文摘To characterize the patterns of ABR waves in tree shrews, we must understand the hearing sensitivity and auditory function of healthy adult tree shrews. Fifteen tree shrews(30 ears) were stimulated with clicks and tone-pips at 11 different frequencies from 1 to 60 kHz. The ABR waves were recorded and analyzed. The ABR consisted of five to seven positive waves in the first 10 ms after a click stimulus, and the average hearing threshold of component III was 27.86 ± 3.78 dB SPL. Wave III was the largest and most clear. The ABR threshold was related to the tone-pip sitmulus by a "U" shaped curve. The sensitive frequency was approximately 8 kHz in tree shrews. The latencies systematically decreased with increasing stimulus frequencies. The ABR amplitudes of wave III increased as the sound pressure level increased. All of these results provide an empirical basis for future studies of hearing diseases in tree shrews.
文摘The auditory brainstern responses(ABR)and middle latency responses(MLR)were recorded in 24 normal mice with a 7S11A processor,and the normalparameters of ABR and MLR obtained.The results showed that the ABR of mice werecomposed of 6 waves,waves Ⅰ to Ⅵ.The mean threshold was 35.12±8.03 dB ,whichwas significantly different from the 22.63 dB of ABR found in guinea pigs.Thelatency of ABR waves Ⅰ to Ⅵizn the mice was found to be 1.30±8.03 ms ;2.02±0.24ms ;2.90±0.27 ms ;3.80±0.24 ms ;4.87±0.42 ms and 5.83±0.40 ms respectively.Nosex or strain differences were found.The MLR waveform of the mice were similar tothose of human and the latency was found to be P0 3.94±1.76 ms;P8.38±1.57ms;Na 14.98±2.54 ms;Pa 24.56±3.15 ms rcspectively.It is suggested that mice can beused for hearing research.Since the Na and Pa waves of MLR were unstable under anes-thesia,testing conditions for determining MLR in mice need further study.
基金supported by grants from the Science and Technology Innovation Nursery Foundation of PLA General Hospital(12KMM29)the military medical metrology research project(2012-JL1-028)+1 种基金Clinical Research Supporting Foundation of PLA General Hospital(2012FC-TSYS-3056)the Military Medical and Sanitation Research Project(11BJZ33)
文摘Objective To analyze the characteristics of auditory brainstem response (ABR) in presbycusis patients el-der than 90 years. Methods Fourteen presbycusis patients elder than 90 years (presbycusis group, 91.1.4 ± 1.3 years, 26 ears) and 9 normal-hearing young adults (control group, 22.7 ± 1.2 years, 18 ears) participated in the study. Alternative click-evoked ABRs were recorded in both groups. The peak latency (PL) of peak I,Ⅲ, and V, and the inter-peak latency (IPI) of I-Ⅲ,Ⅲ-V, and I-V were compared between groups. Results In elder presbycusis patients, the occurrence rate of peak I andⅢwere both 76.9%, and that of peak V was 84.6%. In presbycusis group, the peak latencies of I, Ⅲ, V were significantly longer than that of control group (P<0.001). There was no significant difference between groups in the IPI of peak I-IⅢ (P=0.298, peakⅢ-V (P=0.254) and peak I-V (P=0.364). Conclusions Auditory brainstem responses in presbycusis pa-tients elder than 90 years showed worse wave differentiation.
文摘Objectives To investigate changes in evoked potentials and structure of the guinea pig cochleae during whole cochlear perfusion with glutamate. Methods CM, CAP, DPOAE, and ABR were recorded as indicators of cochlear functions during whole cochlear perfusion. The morphology of the cochlea was studied via transmission electron microscopy. Results There were no significant changes in DPOAE amplitude before and after glutamate perfusion. CM I/O function remained nonlinear during perfusion. ABR latencies were delayed following glutamate perfusion. The average CAP threshold was elevated 35 dB SPL following glutamate perfusion.. The OHCs appeared normal, but the IHCs and afferent dendrites showed cytoplasmic blebs after glutamate perfusion. Conclusions While being a primary amino acid neurotransmitter at the synapses between hair cells and spiral ganglion neurons, excessive glutamate is neurotoxic and can destroy IHCs and spiral ganglion neurons. The technique used in this study can also be used to build an animal model of auditory neuropathy.
文摘The analysis of the Auditory Brainstem Response (ABR) is of fundamental importance to the investigation of the auditory system behavior, though its interpretation has a subjective nature because of the manual process employed in its study and the clinical experience required for its analysis. When analyzing the ABR, clinicians are often interested in the identification of ABR signal components referred to as Jewett waves. In particular, the detection and study of the time when these waves occur (i.e., the wave latency) is a practical tool for the diagnosis of disorders affecting the auditory system. In this context, the aim of this research is to compare ABR manual/visual analysis provided by different examiners. Methods: The ABR data were collected from 10 normal-hearing subjects (5 men and 5 women, from 20 to 52 years). A total of 160 data samples were analyzed and a pair- wise comparison between four distinct examiners was executed. We carried out a statistical study aiming to identify significant differences between assessments provided by the examiners. For this, we used Linear Regression in conjunction with Bootstrap, as a method for evaluating the relation between the responses given by the examiners. Results: The analysis suggests agreement among examiners however reveals differences between assessments of the variability of the waves. We quantified the magnitude of the obtained wave latency differences and 18% of the investigated waves presented substantial differences (large and moderate) and of these 3.79% were considered not acceptable for the clinical practice. Conclusions: Our results characterize the variability of the manual analysis of ABR data and the necessity of establishing unified standards and protocols for the analysis of these data. These results may also contribute to the validation and development of automatic systems that are employed in the early diagnosis of hearing loss.
文摘Tinnitus has often been studied using salicylate in animal models as they are capable of inducing tempo-rary hearing loss and tinnitus. Studies have recently observed enhancement of auditory evoked responses of the auditory cortex (AC) post salicylate treatment which is also shown to be related to tinnitus like behavior in rats. The aim of this study was to observe if enhancements of the AC post salicylate treatment are also present at structures in the brainstem. Four male Sprague Dawley rats with AC implanted electrodes were tested for both AC and auditory brainstem response (ABR) recordings pre and post 250 mg/kg intraperitone-al injections of salicylate. The responses were recorded as the peak to trough amplitudes of P1-N1 (AC), ABR wave V, and ABR waveⅡ. AC responses resulted in statistically significant enhancement of ampli-tude at 2 hours post salicylate with 90 dB stimuli tone bursts of 4, 8, 12, and 20 kHz. Wave V of ABR re-sponses at 90 dB resulted in a statistically significant reduction of amplitude 2 hours post salicylate and a mean decrease of amplitude of 31%for 16 kHz. WaveⅡamplitudes at 2 hours post treatment were signifi-cantly reduced for 4, 12, and 20 kHz stimuli at 90 dB SPL. Our results suggest that the enhancement chang-es of the AC related to salicylate induced tinnitus are generated superior to the level of the inferior colliculus and may originate in the AC.
文摘目的探讨多种听力学检测方法在听性脑干反应(ABR)最大输出未引出患儿的听力学诊断中的应用价值。方法回顾性分析69例(138耳)ABR最大强度未引出患儿的临床资料,年龄42天到5岁,平均1岁6个月,鼓室导抗图均为A型或正向单峰,声反射均未引出,影像学检查内耳无畸形。69例患儿均进行ABR、耳蜗微音电位(CM)、畸变产物耳声发射(DPOAE)和听性稳态反应(ASSR)测试。结果69例138耳中,8例16耳(11.59%)记录到CM,其中10耳(7.25%)记录到DPOAE,0.5、1、2、4 kHz ASSR反应阈值分别为83.2±13.1、82.9±13.0、75.3±12.4、63.1±9.1 dB nHL,结合其他检查结果诊断为听神经病。余61例(122耳)CM和DPOAE均未引出,0.5、1、2、4 kHz的ASSR引出率分别为82.3%、81.9%、76.9%、60.2%,其中20耳ASSR各频率均未引出,102耳至少一个频率引出,0.5、1、2、4 kHz ASSR反应阈分别为93.2±6.1、99.8±7.0、105.4±5.4、108.2±9.8 dB nHL,诊断为极重度感音神经性聋。结论对于ABR最大输出强度未引出的患儿,CM和/或DPOAE引出且ASSR各频率反应阈低于感音神经性聋患儿,有助于听神经病的诊断;CM和DPOAE均未引出有助于极重度感音神经性聋的诊断,ASSR测试有助于评估其残余听力。
文摘目的获取6月龄内婴儿的听性脑干反应（auditory brainstem response，ABR）正常值范围并分析其特点。方法2006年5月至2008年5月对听力正常、出生40天～7月龄的60例婴儿进行ABR检测，根据平均年龄分为A（6周龄）、B（3月龄）、C（6月龄）组，每组20例（40耳）；对照组为听力正常的成人20例（40耳），平均年龄25．2岁。对各组的ABR结果进行统计分析。结果各组100dB nHL ABR的波Ⅰ、Ⅲ、Ⅴ潜伏期的正常值范围分别为：A组：1．49±0．08、4．42±0．16、6．61±0．25ms；B组：1．47±0.07、4．35±0．20、6．50±0．25ms；C组：1．45±0．07、4．17±0．15、6．32±0．22ms；对照组：1．45±0．07、3．79±0．11、5．49±0．20ms；三组婴儿80dB nHL ABR的波Ⅰ、Ⅲ、Ⅴ潜伏期的正常值范围分别为：A组：1．63±0．08、4．52±0．17、6．74±0．26ms；B组：1．64±0．11、4．44±0．20、6．67±0．26ms；C组：1．60±0．11、4．27±0．16、6．43±0．24ms。随刺激声强度降低，ABR各波潜伏期延长。各月龄组婴儿的波Ⅲ、Ⅴ潜伏期、Ⅰ-Ⅲ、Ⅰ—V波间期均比成人延长．且随婴儿月龄增加逐渐缩短，但6月龄时仍未达到成人水平。各月龄组ABR反应阈差异无统计学意义。结论不同月龄婴儿的波Ⅰ潜伏期与成人相近；各月龄婴儿的ABR反应阈无明显差异，且与成人相近。
