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Effects of salvianolic acid-A on NIH/3T3 fibroblast proliferation,collagen synthesis and gene expression 被引量:25
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作者 Liu CH Hu YY +2 位作者 Wang XL Liu P Xu LM 《World Journal of Gastroenterology》 SCIE CAS CSCD 2000年第3期361-364,共4页
AIM To investigate the mechanisms ofsalvianolic acid A(SA-A)against liver fibrosisin vitro.METHODS NIH/3T3 fibroblasts were culturedroutinely,and incubated with 10<sup>-4</sup>mol/L-10<sup>-7</s... AIM To investigate the mechanisms ofsalvianolic acid A(SA-A)against liver fibrosisin vitro.METHODS NIH/3T3 fibroblasts were culturedroutinely,and incubated with 10<sup>-4</sup>mol/L-10<sup>-7</sup>mol/L SA-A for 22 h.The cell viability wasassayed by[<sup>3</sup>H]proline incorporation,cellproliferation by[<sup>3</sup>H]TdR incorporation,cellcollagen synthetic rate was measured with[<sup>3</sup>H]proline impulse and collagenase digestionmethod.The total RNA was prepared from thecontrol cells and the drug treated cellsrespectively,and α(1)I pro-collagen mRNAexpression was semi-quantitatively analyzedwith RT-PCR.RESULTS 10<sup>-4</sup>mol/L SA-A decreased cellviability and exerted some cytotoxiciy,while10<sup>-5</sup>mol/L-10<sup>-7</sup>mol/L SA-A did not affect cellviability,but inhibited cell proliferationsignificantly,and 10<sup>-6</sup>mol/L SA-A had the besteffect on cell viability among theseconcentrations of drugs.10<sup>-5</sup>mol/L-10<sup>-6</sup>mol/LSA-A inhibited intracellular collagen syntheticrate,but no significant influence on extracellularcollagen secretion.Both 10<sup>-5</sup>mol/L and10<sup>-6</sup>mol/L SA-A could decrease α(1)I pro-collagen mRNA expression remarkably.CONCLUSION SA-A had potent action againstliver fibrosis.It inhibited NIH/3T3 fibroblastproliferation,intracellular collagen syntheticrate and type I pro-collagen gene expression,which may be one of the main mechanisms of thedrug. 展开更多
关键词 salvianolic acid-a NIH/3T3 FIBROBLAST CELL VIABILITY CELL proliferation COLLAGEN gene expression
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STUDIES ON THE SYNTHESIS OF SINULARIOE-D,SINULARIAL-A AND SINULARIC ACID-A
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作者 Yu Lin LI Ya Cheng XING Xiang Jun YUE The State Key Laboratory of Applied Organic Chemistry and Institute of Organic Chemistry,Lanzhou University,Lanzhou 730000. 《Chinese Chemical Letters》 SCIE CAS CSCD 1992年第9期681-682,共2页
A precursor 12 of sinulariol-D,sinularial-A and sinularic acid-A(marine cembranoids)was prepared from geraniol through nine steps
关键词 OH PH ACID PPM STUDIES ON THE SYNTHESIS OF SINULARIOE-D SINULARIAL-A AND SINULARIC acid-a
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非自身标样定量测定DNTF的分析方法 被引量:1
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作者 罗西 刘红妮 +4 位作者 苏鹏飞 张皋 胡银 杨彩宁 刘可 《火炸药学报》 EI CAS CSCD 北大核心 2019年第1期94-96,102,I0006,共5页
为了在标准物质缺乏的情况下能够准确定量3,4-二硝基呋咱基氧化呋咱(DNTF),采用气相色谱-原子发射光谱(GC-AED)联用技术,建立了一种DNTF的非含能非自身标样定量测定方法;通过对比DNTF和5种非含能材料(二苯胺、苯甲酸、乙酰苯胺、DBS、D... 为了在标准物质缺乏的情况下能够准确定量3,4-二硝基呋咱基氧化呋咱(DNTF),采用气相色谱-原子发射光谱(GC-AED)联用技术,建立了一种DNTF的非含能非自身标样定量测定方法;通过对比DNTF和5种非含能材料(二苯胺、苯甲酸、乙酰苯胺、DBS、DBP)的C元素标准曲线,选择相对定量响应因子最接近1的非含能材料作外标物定量DNTF的C元素,再结合分子式对DNTF进行定量。结果表明,苯甲酸的相对定量响应因子最接近1,用苯甲酸的C元素标准曲线定量DNTF的C元素,得到DNTF的C元素线性范围为17.43~426.92μg/mL,相关系数(r)为0.9992;以3倍信噪比计算检测限,得到DNTF的C元素方法检测限为0.0096μg/mL;相同条件下,DNTF在线性范围内高、中、低浓度下的加标回收率分别为104.9%,103.7%和109.4%,相对标准偏差分别为3.3%、1.1%和1.5%;该方法具有较好的准确度和精密度,可以实现在自身标准物质缺乏的情况下用非含能材料作外标物对DNTF的准确定量。 展开更多
关键词 分析化学 高能量密度材料 气相色谱-原子发射光谱 CIC法 3 4-二硝基呋咱基氧化呋咱(DNTF) 苯甲酸
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