目的:探讨去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)蛋白在人乳腺癌细胞系中的表达。方法:提取人正常乳腺上皮细胞HBL100,人乳腺癌细胞系MCF-7,ZR-75-1,MDA-MB-231,HCC1937和Hs578T,小鼠乳腺癌细胞系4T1的总...目的:探讨去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)蛋白在人乳腺癌细胞系中的表达。方法:提取人正常乳腺上皮细胞HBL100,人乳腺癌细胞系MCF-7,ZR-75-1,MDA-MB-231,HCC1937和Hs578T,小鼠乳腺癌细胞系4T1的总蛋白和总mRNA,提取细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测ADAM9的表达。结果:在人乳腺癌细胞系MDA-MB-231中ADAM9蛋白表达最高,在小鼠乳腺癌4T1细胞中ADAM9表达较低。结论:ADAM9在高侵袭的MDA-MB-231乳腺癌细胞中高表达。展开更多
去整合素–金属蛋白酶家族(adisintegrin and metalloproteinase, ADAMs)是一类锌依赖的跨膜糖蛋白,ADAMs家族分子具有相似的分子结构包括:结构域(单肽)末端金属域、整合素域、富含半胱氨酸的序列、膜近端上皮生长因子样序列。ADAM9在...去整合素–金属蛋白酶家族(adisintegrin and metalloproteinase, ADAMs)是一类锌依赖的跨膜糖蛋白,ADAMs家族分子具有相似的分子结构包括:结构域(单肽)末端金属域、整合素域、富含半胱氨酸的序列、膜近端上皮生长因子样序列。ADAM9在胶质瘤、黑色素瘤、前列腺癌、胰腺导管腺癌、胃癌、乳腺癌、肺癌和肝癌等实体肿瘤中均有过表达。免疫组织化学分析强调了ADAM9在实际癌细胞中的表达,并将其丰富的表达与临床病理特征联系起来,如总体生存期缩短、肿瘤分级较差、去分化、治疗耐药性和转移形成。本文综述了ADAM9在肿瘤中的研究现状,以及它在推动肿瘤进展方面的不同机制。展开更多
目的观察去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)在小鼠肾发育中的时空表达,从而探讨ADAM9与肾发育的关系。方法采用免疫组织化学结合体视学方法和免疫印迹法,测定胚龄11、14、16、18d及生后1、3、7、14、28...目的观察去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)在小鼠肾发育中的时空表达,从而探讨ADAM9与肾发育的关系。方法采用免疫组织化学结合体视学方法和免疫印迹法,测定胚龄11、14、16、18d及生后1、3、7、14、28、40d小鼠肾组织内ADAM9的表达以及含量变化。结果免疫组织化学显示ADAM9在输尿管芽以及各期肾小体、皮质肾小管均有表达,生后1d后髓质小血管有表达,集合管表达始终较弱;细胞图像分析和体视学测量显示随着胚日龄的增加,ADAM9在肾小体的表达逐渐增强,后趋于稳定;肾小管的表达则呈先增后减的趋势;免疫印迹显示ADAM9在肾的表达量在生后7d达到高峰,随后逐渐减弱。结论 ADAM9对肾的早期发育起重要作用。展开更多
The 'a disintegrin and metalloprotease' (ADAM) family contributes to regulation of the cell-cell and cell-matrix interactions that are critical determinants of malignancy. To determine the relationship between...The 'a disintegrin and metalloprotease' (ADAM) family contributes to regulation of the cell-cell and cell-matrix interactions that are critical determinants of malignancy. To determine the relationship between metastasis and ADAM proteins, we compared the mRNA levels of ADAM9,-10,-12,-15, and-17 in sublines of an EBC-1 lung cancer cell line that were highly metastatic to either brain or bone. ADAM9 mRNA levels were significantly higher in highly brain-metastatic sublines than in the parent or highly bonemetastalic sublines. To elucidate the role ofADAM9 in brain metastasis, we stably transfected A549 and EBC-1 cells with a fulllength ADAM9 expression vector. Compared with mock-transfectants, ADAM9 overexpression resulted in increased invasive capacity展开更多
文摘目的:探讨去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)蛋白在人乳腺癌细胞系中的表达。方法:提取人正常乳腺上皮细胞HBL100,人乳腺癌细胞系MCF-7,ZR-75-1,MDA-MB-231,HCC1937和Hs578T,小鼠乳腺癌细胞系4T1的总蛋白和总mRNA,提取细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测ADAM9的表达。结果:在人乳腺癌细胞系MDA-MB-231中ADAM9蛋白表达最高,在小鼠乳腺癌4T1细胞中ADAM9表达较低。结论:ADAM9在高侵袭的MDA-MB-231乳腺癌细胞中高表达。
文摘去整合素–金属蛋白酶家族(adisintegrin and metalloproteinase, ADAMs)是一类锌依赖的跨膜糖蛋白,ADAMs家族分子具有相似的分子结构包括:结构域(单肽)末端金属域、整合素域、富含半胱氨酸的序列、膜近端上皮生长因子样序列。ADAM9在胶质瘤、黑色素瘤、前列腺癌、胰腺导管腺癌、胃癌、乳腺癌、肺癌和肝癌等实体肿瘤中均有过表达。免疫组织化学分析强调了ADAM9在实际癌细胞中的表达,并将其丰富的表达与临床病理特征联系起来,如总体生存期缩短、肿瘤分级较差、去分化、治疗耐药性和转移形成。本文综述了ADAM9在肿瘤中的研究现状,以及它在推动肿瘤进展方面的不同机制。
文摘The 'a disintegrin and metalloprotease' (ADAM) family contributes to regulation of the cell-cell and cell-matrix interactions that are critical determinants of malignancy. To determine the relationship between metastasis and ADAM proteins, we compared the mRNA levels of ADAM9,-10,-12,-15, and-17 in sublines of an EBC-1 lung cancer cell line that were highly metastatic to either brain or bone. ADAM9 mRNA levels were significantly higher in highly brain-metastatic sublines than in the parent or highly bonemetastalic sublines. To elucidate the role ofADAM9 in brain metastasis, we stably transfected A549 and EBC-1 cells with a fulllength ADAM9 expression vector. Compared with mock-transfectants, ADAM9 overexpression resulted in increased invasive capacity
