拟南芥AtJ3基因的克隆和分析

克隆并分析了拟南芥（Ａｒａｂｉｄｏｐｓｉｓｔｈａｌｉａｎａ（Ｌ．）Ｈｅｙｎｈ．）ＡｔＪ３的ｃＤＮＡ核苷酸序列，并证明其翻译产物与Ｅ．ｃｏｌｉ的ＤｎａＪ蛋白高度同源。ＡｔＪ３蛋白分子中具有全部这类蛋白的典型特征包括Ｊ结构域（ｄｏｍａｉｎ）、Ｇ或ＧＦ结构域、富含半胱氨酸的锌指结构域，Ｃ末端的ＣＡＱＱ是一个蛋白法尼基化信号。用ＡｔＪ３的ｃＤＮＡ序列末端非翻译区作探针，从拟南芥基因组文库中分离获得ＡｔＪ３基因。基因序列分析表明该基因是由被５个内含子分隔的６个外显子组成。根据Ｓｏｕｔｈｅｒｎ杂交分析，ＡｔＪ３基因为单拷贝基因。Ｎｏｒｔｈｅｒｎ分析结果表明，ＡｔＪ３在子叶、叶、根、花及长角果中都表达。

Toll样受体4在胃食管交界部腺癌发展中的作用及与血管密度的关系

目的 :探讨Toll样受体4(TLR4)在胃食管交界部腺癌发展中的作用及其与血管生成之间的关系。方法 :采用回顾分析,通过免疫组化法检测43例(其中23例为随访证实已出现复发转移)经病理证实的胃食管交界部腺癌手术切除标本中TLR4蛋白的表达;采用CD34抗体标记微血管内皮细胞,计算微血管密度(MVD)。分析TLR4蛋白的表达、MVD与胃食管交界部腺癌主要临床病理参数以及预后的关系。结果:TLR4蛋白的阳性表达率为69.8%,其中A组(复发转移组)TLR4蛋白阳性率为82.6%,明显高于对照组B组(55.0%,P<0.05);有淋巴结转移者的TLR4蛋白阳性表达率(79.3%)高于无淋巴结转移者(50.0%,P=0.05);TNM分期为Ⅲ+Ⅳ期者的TLR4蛋白阳性表达率(82.8%),明显高于Ⅰ+Ⅱ期者(42.9%,P<0.01)。TLR4蛋白的表达与MVD呈正相关(P=0.008)。结论:TLR4的高表达与胃食管交界部腺癌的发生发展及患者的预后情况密切相关,可能与TLR4促进肿瘤微血管生成相关。

STAT3在食管胃交界部腺癌中的表达及意义

目的检测信号转导与转录激活因子3(STAT3)在食管胃交界部腺癌中的表达,探讨其在癌症中的作用。方法采用免疫组织化学的方法,检测2015年6月至2016年12月被河南省肿瘤医院确诊为进展期食管胃交界部腺癌的67例癌症患者,及35例癌旁正常对照组织中STAT3及p-STAT3的表达,用t检验的方法分析两组间STAT3及p-STAT3的表达差异。结果 45例(67.2%)食管胃交界部腺癌高表达STAT3,22例(32.8%)低表达STAT3;34(50.7%)例高表达p-STAT3,33例(49.3%)低表达p-STAT3。且癌组织分化程度越高,STAT3及p-STAT的表达水平则越高。结论 STAT3可能与食管胃交界处腺癌的发生、发展有关,或可成为新的治疗靶点之一。

CXCL1在食管胃交界处腺癌中的表达及与患者预后的关系

目的检测趋化因子1(CXCL1)在食管胃交界部腺癌中的表达,探讨其在癌症预后评价中的作用。方法采用免疫组织化学的方法,检测2010年至2012年被河南省肿瘤医院确诊为食管胃交界部腺癌且有组织蜡块的121例癌症患者组织中CXCL1的表达,并经随访统计各位患者生存期情况。用χ2检验的方法比较CXCL1的表达差异,用Kaplan Meier法分析其生存情况,并用Log rank test比较,预后分析采用多因素Cox回归。结果 92例(76.03%)为CXCL1阳性表达,29例(23.97%)为阴性表达,且CXCL1的表达在高中分化、III或IV期及有淋巴结转移的患者中,均显著增高。CXCL1表达阳性的患者,无病生存期及总生存期较表达阴性的患者显著缩短,且经Cox多因素分析,CXCL1的表达、癌症分化程度、分期、淋巴结的转移都是影响无病存活期与总生存期的重要因素。结论 CXCL1可能与食管胃交界处腺癌的发生、发展及预后有关,或可作食管胃交界部腺癌判断预后及治疗的一个新的分子靶点。

Influence of Sea Level Pressure on Inter-Annual Rainfall Variability in Northern Senegal in the Context of Climate Change

This study examines the inter-annual variability of rainfall and Mean Sea Level Pressure (MSLP) over west Africa based on analysis of the Global Precipitation Climatology Project (GPCP) and National Center for Environmental Prediction/National Center for Atmospheric Research (NCEP/NCAR) Reanalysis respectively. An interconnection is found in this region, between Mean Sea Level Pressure (MSLP) anomaly (over Azores and St. Helena High) and monthly mean precipitation during summer (June to September: JJAS). We also found that over northern Senegal (15°N - 17°N;17°W - 13°W) the SLP to the north is strong;the wind converges at 200 hPa corresponding to the position of the African Easterly Jet (AEJ) the rotational wind 700 hPa (corresponding to the position of the African Easterly Jet (AEJ) coming from the north-east is negative. In this region, the precipitation is related to the SLP to the north with the opposite sign. The Empirical Orthogonal Functions (EOF) of SLP is also presented, including the mean spectrum of precipitation and pressures to the north (15°N - 40°N and 50°W - 25°W) and south (40°S - 10°S and 40°W - 0°E). The dominant EOF of Sea Level Pressures north and south of the Atlantic Ocean for GPCP represents about 62.2% and 69.4% of the variance, respectively. The second and third EOFs of the pressure to the north account for 24.0% and 6.5% respectively. The second and third EOFs of the pressure to the south represent 12.5% and 8.9% respectively. Wet years in the north of Senegal were associated with anomalous low-pressure areas over the north Atlantic Ocean as opposed to the dry years which exhibited an anomalous high-pressure area in the same region. On the other hand, over the South Atlantic, an opposition is noted. The wavelet analysis method is applied to the SLP showings to the north, south and precipitation in our study area. The indices prove to be very consistent, especially during intervals of high variance.