基于AFP法的森林生态旅游风险评估——以黄崖洞国家森林公园为例

在生态旅游及项目风险管理等相关理论的基础上，运用AFP研究方法，构建了森林生态旅游风险评估体系。以黄崖洞国家森林公园为实证研究对象，选取了6个一级指标及22个二级指标，通过模糊综合评价计算出各个指标的风险等级，最后提出了风险防范措施。

制造专家系统的AFP评价法

本文分析了制造专家系统的特征因素，并对这些特征因素进行了权衡分析．在此基础上，对传统的层次分析法的不足做了剖析，建立了制造专家系统的ＡＦＰ评价模型，最后，对ＡＨＰ法评价案例进行了ＡＦＰ法的讨论．

SHCSP与AFP检测肝癌研究

本文用SHCSP与AFP检测原发性肝癌126例,转移性肝癌6例,非肝癌患者141例,SHCSP法诊断敏感性90.48％,特异性90.07％,诊断效率90.26％,阳漏本9.52％,假阳性9.93％,LR(+)=9.11,LR(-)=0.11。而AFP法诊断敏感性70.63％,特异位88.65％,诊断效率80.15％,阳漏率29.37％,假阳性11.35％,LR(+)=6.22,LR(-)=0.33,检测SHCSP诊断肝癌比AFP优,且有非常显著性差别(P<0. 01),两法在诊断肝癌和排除肝癌有互补性,应联合检测,以提高肝癌诊断率。

Multimarker Detection of MAGE-1,MAGE-3 and AFP mRNAs by a Real-time Quantitative PCR Assay:a Possible Predictor of Hematogenous Micrometastasis of Hepatocellular Carcinoma

OBJECTIVE To explore the relationship between multimarker detection of MAGE-1,MAGE-3 and AFP mRNAs in the peripheral blood of patients with hepatocellular carcinoma and micrometastasis using a realtime quantitative-PCR(real-time Q-PCR)assay. METHODS Peripheral blood samples were obtained from control subjects and 86 patients with hepatocellular carcinoma (HCC).Real-time Q-PCR was used to detect MAGE-1,MAGE-3, and AFP mRNAs in the blood cells. RESULTS In 86 tumor specimens,the positivity for MAGE-1, MAGE-3,and AFP genes was respectively 34.9%(30/86),60.5% (52/86)and 69.8%(60/86).All specimens expressed at least one marker.MAGE-1,MAGE-3,and AFP transcripts were detected respectively in 12(14.0%),18(20.1%)and 29(33.7%)of the 86 blood specimens from hepatocellular carcinoma patients,while 45 specimens(52.3%)were positive for at least one marker.In addition,MAGE-1,MAGE-3 and AFP gene transcripts were not detected in any peripheral blood specimens from 25 chronic liver disease patients and 28 normal healthy volunteers.The positive rate correlated with the TNM clinical stages,extrahepatic metastasis and portal vein carcinothrombosis(P<0.05).No correlation was found between tumor size,tumor number, differentiation,serum a-fetoprotein(AFP)and the positive rate. CONCLUSION Our results indicate that a multimarker real- time Q-PCR assay with cancer-specific markers such as MAGE-1 and MAGE-3 in combination with a hepatocyte-specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity.

三磷酸腺苷法在指导膀胱移行细胞癌术后膀胱灌注化疗中的体外敏感性研究

目的：探讨应用三磷酸腺苷生物荧光抗癌药物敏感性测验（ATP—TCA）技术指导膀胱癌进行化疗。方法：采用ATP对19例病人进行肿瘤药敏试验，根据实验结果进行个体化膀胱灌注治疗。并与同期26例使用比柔比星进行膀胱灌注化疗的病人作为对照组，观察两组病人术后肿瘤的复发情况。结果：（1）药敏组和对照组术后肿瘤的复发率差异具有统计学意义（2）药敏组中出现多重耐药相关性具有统计学意义。结论：采用ATP—TCA进行体外药物敏感性实验后采取个体化灌注化疗能够减少TCCB的复发。