Introduction:Among all malignant tumors of the digestive system,pancreatic carcinoma exhibits the highest mortality rate.Currently,prevention and effective treatment are urgent issues that need to be addressed.Methods...Introduction:Among all malignant tumors of the digestive system,pancreatic carcinoma exhibits the highest mortality rate.Currently,prevention and effective treatment are urgent issues that need to be addressed.Methods:The study focused on meiotic nuclear divisions 1(MND1),integrating data from the Gene Expression Profiling Interactive Analysis(GEPIA)database with prognostic survival analysis.Simultaneously,experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC.The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA(siRNA)in Patu-8988 and Panc1 cell lines.Results:The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell proliferation.Wound healing and Transwell assays revealed that MND1 knockdown reduced cell migration and invasion.Flow cytometry revealed that inhibiting MND1 hindered the cell cycle.Furthermore,MND1 could stimulate the proliferation,migration,and invasion of Patu-8988 and Panc1 cells by increasing the expression of MND1.Notably,MND1 had a positive effect on H2AFX expression in PC cells.Elevated MND1 expression suggests the low overall survival rate of individuals diagnosed with PC.Conclusion:These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC.展开更多
二向反射分布函数包含地表反射的方向性特征信息。研究二向反射分布函数BRDF(Bidirectional Reflectance Distribution Function)形状对植被结构参数的敏感性,有助于理解植被的二向性反射规律,进而反演植被参数。本文耦合双冠层反射率...二向反射分布函数包含地表反射的方向性特征信息。研究二向反射分布函数BRDF(Bidirectional Reflectance Distribution Function)形状对植被结构参数的敏感性,有助于理解植被的二向性反射规律,进而反演植被参数。本文耦合双冠层反射率模型和核驱动的罗斯厚核-李氏稀疏互易核模型,利用EFAST全局敏感性分析方法,以各向异性平整指数为BRDF形状变化的衡量指标,研究了不同天空光比例(SKYL)下,各向异性平整指数AFX对植被参数敏感度的变化,以及SKYL=0.1时AFX的敏感性。结果表明:(1)在红波段,上、下层叶面积指数、上层叶绿素含量,以及上层叶倾角分布是AFX的敏感参数,在近红外波段,上、下层叶面积指数LAI是AFX的敏感参数。(2)冠层尺度上的参数敏感度总体大于叶片尺度。(3)晴天时(SKYL=0.1),红波段主敏感度较大的参数分别是上层LAI、上层叶倾角分布和下层叶片结构参数,近红外波段主敏感度较大的参数主要是上、下层LAI。展开更多
聚集指数CI(Clumping Index)是植被冠层的一个重要结构参数,对植被冠层的辐射截获,以及全球碳、水循环的研究均有重要作用。现有星载CI产品的估算主要是基于CI-NDHD(Normalized Difference between Hotspot and Dark spot)线性模型方法...聚集指数CI(Clumping Index)是植被冠层的一个重要结构参数,对植被冠层的辐射截获,以及全球碳、水循环的研究均有重要作用。现有星载CI产品的估算主要是基于CI-NDHD(Normalized Difference between Hotspot and Dark spot)线性模型方法,由于针叶林和阔叶林在叶片尺度上存在聚集层级的差异,该模型对它们分别采用了不同的模型系数。但是,该模型对中粗分辨率的针阔混交林像元通常采用阔叶林的CI反演系数,因此,理论上会导致该类型CI的高估。为此,本文提出了一种动态选取混交林像元端元CI组分的方法,以改进针阔混交林植被聚集指数的估算精度。首先,通过国际地圈—生物圈计划(IGBP)的地表类型和描述二向性反射分布函数BRDF(Bidirectional Reflectance Distribution Function)特征的地表各向异性平整指数AFX(Anisotropic Flat Index)进行双重约束,逐像元地计算端元CI值;然后,结合高分辨率的土地覆盖分类数据确定端元在像元中的面积比例,并估算MODIS针阔混交林像元的聚集指数MFCI(Mixed Forest CI);最后,将方法应用于研究区MODIS数据的MFCI估算,并通过地面实测数据进行精度评价。结果表明:目前的MODIS产品算法高估了针阔混交林像元的CI值,而MFCI估算方法在CI-NDHD算法的基础上,可以较显著地改善该类型聚集指数的估算精度,当针叶林树种成数达到60%时,精度改善可达28.03%,其中,改进结果的均方根误差(RMSE)和偏差(Bias)各降低约84%和175%。研究表明,MFCI方法对针阔混合像元的端元组分的变化敏感,在高分辨率地表分类已知的条件下,MFCI方法为针阔混交林CI产品生产和精度提高提供了可行的解决方案。展开更多
基金supported by grants from National Innovation Program for College Students(202210367076)Graduate Student Research Innovation Program of Bengbu Medical College(Byycxz22016)the National Natural Science Foundation of China(82072585),and the Key Research Project of Bengbu Medical College(No.2020byzd029).
文摘Introduction:Among all malignant tumors of the digestive system,pancreatic carcinoma exhibits the highest mortality rate.Currently,prevention and effective treatment are urgent issues that need to be addressed.Methods:The study focused on meiotic nuclear divisions 1(MND1),integrating data from the Gene Expression Profiling Interactive Analysis(GEPIA)database with prognostic survival analysis.Simultaneously,experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC.The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA(siRNA)in Patu-8988 and Panc1 cell lines.Results:The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell proliferation.Wound healing and Transwell assays revealed that MND1 knockdown reduced cell migration and invasion.Flow cytometry revealed that inhibiting MND1 hindered the cell cycle.Furthermore,MND1 could stimulate the proliferation,migration,and invasion of Patu-8988 and Panc1 cells by increasing the expression of MND1.Notably,MND1 had a positive effect on H2AFX expression in PC cells.Elevated MND1 expression suggests the low overall survival rate of individuals diagnosed with PC.Conclusion:These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC.
文摘二向反射分布函数包含地表反射的方向性特征信息。研究二向反射分布函数BRDF(Bidirectional Reflectance Distribution Function)形状对植被结构参数的敏感性,有助于理解植被的二向性反射规律,进而反演植被参数。本文耦合双冠层反射率模型和核驱动的罗斯厚核-李氏稀疏互易核模型,利用EFAST全局敏感性分析方法,以各向异性平整指数为BRDF形状变化的衡量指标,研究了不同天空光比例(SKYL)下,各向异性平整指数AFX对植被参数敏感度的变化,以及SKYL=0.1时AFX的敏感性。结果表明:(1)在红波段,上、下层叶面积指数、上层叶绿素含量,以及上层叶倾角分布是AFX的敏感参数,在近红外波段,上、下层叶面积指数LAI是AFX的敏感参数。(2)冠层尺度上的参数敏感度总体大于叶片尺度。(3)晴天时(SKYL=0.1),红波段主敏感度较大的参数分别是上层LAI、上层叶倾角分布和下层叶片结构参数,近红外波段主敏感度较大的参数主要是上、下层LAI。
文摘聚集指数CI(Clumping Index)是植被冠层的一个重要结构参数,对植被冠层的辐射截获,以及全球碳、水循环的研究均有重要作用。现有星载CI产品的估算主要是基于CI-NDHD(Normalized Difference between Hotspot and Dark spot)线性模型方法,由于针叶林和阔叶林在叶片尺度上存在聚集层级的差异,该模型对它们分别采用了不同的模型系数。但是,该模型对中粗分辨率的针阔混交林像元通常采用阔叶林的CI反演系数,因此,理论上会导致该类型CI的高估。为此,本文提出了一种动态选取混交林像元端元CI组分的方法,以改进针阔混交林植被聚集指数的估算精度。首先,通过国际地圈—生物圈计划(IGBP)的地表类型和描述二向性反射分布函数BRDF(Bidirectional Reflectance Distribution Function)特征的地表各向异性平整指数AFX(Anisotropic Flat Index)进行双重约束,逐像元地计算端元CI值;然后,结合高分辨率的土地覆盖分类数据确定端元在像元中的面积比例,并估算MODIS针阔混交林像元的聚集指数MFCI(Mixed Forest CI);最后,将方法应用于研究区MODIS数据的MFCI估算,并通过地面实测数据进行精度评价。结果表明:目前的MODIS产品算法高估了针阔混交林像元的CI值,而MFCI估算方法在CI-NDHD算法的基础上,可以较显著地改善该类型聚集指数的估算精度,当针叶林树种成数达到60%时,精度改善可达28.03%,其中,改进结果的均方根误差(RMSE)和偏差(Bias)各降低约84%和175%。研究表明,MFCI方法对针阔混合像元的端元组分的变化敏感,在高分辨率地表分类已知的条件下,MFCI方法为针阔混交林CI产品生产和精度提高提供了可行的解决方案。
