Silencing MTA1 by RNAi Reverses Adhesion, Migration and Invasiveness of Cervical Cancer Cells (SiHa) via Altered Expression of p53, and E-cadherin/β-catenin Complex

It has been reported that metastasis-associated gene 1 （Mta1） is overexpressed in many malignant tumors with high metastatic potential. In addition, some studies indicated that MTA1 participated in invasion, metastasis, and survival of cancer cells by regulating cell migration, adhesion and proliferation. But the role of MTA1 is unclear in vitro in the development of cervical cancer cells. This study investigated whether and how MTA1 mediated cell proliferation, migration, invasion and adhesion in cervical cancer. MTA1 expression level was detected by Western blot in two cervical cancer cell lines of different invasion potentials. The effects of MTA1 expression on SiHa cell apoptosis, cycle, proliferation, migration, invasion and adhesion were tested by flow cytometry, MTT, wound-healing assay, Transwell assay and adhesion assay, respectively. The expression levels of p53, E-cadherin, and β-catenin activity were evaluated in untreated and treated cells. The results showed that MTA1 protein expression was significantly higher in SiHa than in HeLa, which was correlated well with the potential of migration and invasion in both cell lines. Furthermore, the cell invasion, migration and adhesion capabilities were decreased after inhibition of MTA1 expression mediated by Mta1-siRNA transfection in SiHa. However, no significant differences were found in cell apoptosis, cycle, and proliferation. In addition, E-cadherin and p53 protein levels were significantly up-regulated, while β-catenin was significantly down-regulated in SiHa transfected with the siRNA. These results demonstrated that MTA1 played an important role in the migration and invasion of cervical cancer cells. It was speculated that the decreased migration and invasion capability by inhibiting the MTA1 expression in the SiHa cell line may be mediated through the altered expression of p53, and E-cadherin/β-catenin complex. MTA1 could serve as a potential therapeutic target in cervical cancer.

Correlation of serum STMN1 and Cath-D levels with the invasive growth of cervical cancer

Objective:To study the correlation of serum stathmin 1 (STMN1) and cathepsin D (Cath-D) levels with the invasive growth of cervical cancer.Methods: The patients with cervical cancer and those with uterine fibroids who underwent surgical resection in the Second People's Hospital of Yichang between January 2013 and November 2017 were selected and enrolled in the malignant group and the benign group of the study respectively;the serum was collected before operation to detect the contents of STMN1 and Cath-D;the cervical cancer lesion tissues were collected from the malignant group and the normal cervical tissues were collected from the benign group to measure the expression of STMN1, Cath-D, invasion genes and invasion signal molecules.Results:STMN1 and Cath-D contents in serum as well as STMN1 and Cath-D mRNA expression in lesion tissue of malignant group were significantly higher than those of benign group, and serum STMN1 and Cath-D contents of malignant group were positively correlated with STMN1 and Cath-D mRNA expression in lesion tissue;RACK1, Twist, MMP9, mTOR, S6K1, Wnt andβ-catenin mRNA expression in lesion tissue of malignant group were significantly higher than those of benign group whereas E-cadherin, GRIM19 and GSK-3β mRNA expression were significantly lower than those of benign group, and serum STMN1 and Cath-D contents of malignant group were positively correlated with RACK1, Twist, MMP9, mTOR, S6K1, Wnt andβ-catenin mRNA expression in lesion tissue, and negatively correlated with E-cadherin, GRIM19 and GSK-3β mRNA expression. Conclusion:The abnormally elevated STMN1 and Cath-D in serum of patients with cervical cancer can evaluate the invasive growth of the lesion.

Expression of MAPK1 in cervical cancer and effect of MAPK1 gene silencing on epithelial-mesenchymal transition,invasion and metastasis

Objective:To discuss the expression of mitogen-activated protein kinase 1(MAPK1) in the cervical cancer and effect of MAPK1 gene silencing on epithelial-mesenchymal transition and invasion and metastasis.Methods:Immunohistoehemistry,western blot and RT-PCR method were employed to detect the expression of MAPKl protein and mRNA in cervical cancer tissue and adjacent normal tissue.The constructed siRNA-MAPKI was transferred into human cervical cancer HeLa cells using Lipofectamine^(?)2000.MTT method was used to detect the cell vitality,transwell method to detect the cell invasion,and western blot to detect the expression of matrix metalloproteinases(MMP)-2,MMP-9,tissue inhibitor of metalloproteinase(TIMP)-1,TIMP-2,zinc finger transcription factor(Snail),epithelialmesenchymal transition related protein(EMT) E-cadherin and vimentin in cells.Results:The expression of MAPKl protein and mRNA in the cervical cancer tissue was significantly higher than the one in the adjacent normal tissue(P<0.01):after transfecting the siRNA-MAPKI into the human cervical cancer HeLa cells through liposome,compared with the control group,its cell vitality was significantly decreased(P<0.01),cell invasion was significantly decreased(P<0.01);expressed of MMP2.MMP-9,Snail and vimentin was significantly decreased(P<0.01),and expression of TIMP-1,TIMP-2 and E-cadherin was significantly increased(/J<0.01).Conclusions:Because of the high expression of MAPKl in the cervical cancer tissue,the interference in the expression of MAPK1 can significantly inhibit the invasion and metastasis of cervical cancer HeLa cells,which is related to the interference in the expression of MMPs/TIMP and Snail-mediated generation of EMT.

Effects of Curcumin on Invasion and Metastasis in the Human Cervical Cancer Cells Caski

Objective： To explore the effects of curcumin on invasion and metastasis in the human cervical cancer cells Caski. Methods： Caski cells were treated with 10, 25, 50tamol/L curcumin for 24, 48, 72 h. Proliferation of Caski cells was measured with MTT assay. When treated with 50pmol/L curcumin for 72 h, the expressions of MMP-2, MT1-MMP and NF-κB of cells were detected by Western-blot, and invasion and metastasis of Caski cells were evaluated with transwell chamber. Results： After being treated with 10μmol/L, 251xmol/L, 50μmol/L curcumin for 24, 48 and 72 h, the proliferation of Caski cells was inhibited in a dose-and time-dependent manner. The expression of MMP-2, MT1-MMP and NF-nB were decreased when being treated with 50μmol/L curcumin for 72 h. After treatment with 50μmol/L curcumin, in invasion assay, the number of cells in curcumin treated group to migrate to filter coated with Matrigel was reduced compared with control group（P〈0.05）. Meanwhile, in migration assay, the number of cells in curcumin treated group to migrate to filter was also decreased compared with control group （P〈0.05）. Conclusion： Curcumin could affect the invasion and metastasis of the human cervical cancer cells Caski. Inhibiting the expression of MMP-2, MT1-MMP and NF-κB was probably one of its molecular mechanisms.

Effects of high-intensity focused ultrasound combined with interventional chemoembolization on the advanced cervical cancer lesion growth and cell invasion

Objective:To study the effects of high-intensity focused ultrasound (HIFU) combined with interventional chemoembolization on the advanced cervical cancer lesion growth and cell invasion.Methods:Patients with stage IIB-IVA cervical cancer treated in Suining Hospital of TCM between May 2014 and October 2016 were selected and randomly divided into two groups, HIFU group received HIFU combined with interventional chemoembolization, and the control group accepted interventional chemoembolization. The levels of tumor markers in serum as well as the expression of tumor suppressor genes and invasion genes in tumor lesions were determined before and after treatment.Results: 2 weeks, 3 weeks and 4 weeks after treatment, serum TK-1, SCC and CA125 levels of both groups were lower than those before treatment, serum TK-1, SCC and CA125 levels of HIFU group 2 weeks after treatment were not different from those of control group, and serum TK-1, SCC and CA125 levels of HIFU group 3 weeks and 4 weeks after treatment were significantly lower than those of control group;4 weeks after treatment, AIF, NDRG4, SARI and eIF4E3 mRNA expression in tumor lesions of both groups were higher than those before treatment while FAK, KGFR and MMP9 mRNA expression were lower than those before treatment, and AIF, NDRG4, SARI and eIF4E3 mRNA expression in tumor lesions of HIFU group were higher than those of control group while FAK, KGFR and MMP9 mRNA expression were lower than those of control group.Conclusion: HIFU combined with interventional chemoembolization can be more effective in suppressing the advanced cervical cancer lesion growth and cell invasion than interventional chemoembolization alone.

Value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer

Objective: To study the value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer. Methods: Patients with cervical cancer who underwent surgical resection in Yan'an University Affiliated Hospital between March 2015 and May 2017 were selected as cervical cancer group of the research, and patients with benign lesions who underwent hysterectomy in Yan'an University Affiliated Hospital during the same period were selected as the control group of the research. Before operation, cervical exfoliated cytology was taken for DNA ploid detection;before operation, the cervical tissue was taken to determine the expression of proliferation genes and invasion genes. Results: The number of exfoliated cells with abnormal DNA ploid in cervical cancer group was significantly higher than that in control group;Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue of cervical cancer group were significantly higher than those of control group whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those of control group;Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue with 3 exfoliated cells with abnormal DNA ploid were significantly higher than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid. Conclusion: DNA ploid detection can be used to evaluate the proliferation and invasion of cancer cells in cervical cancer.

Twist and YB-1 gene expression in cervical cancer and precancerous tissue and their correlation with cell invasion

Objective:To study the correlation of Twist and YB-1 gene expression in cervical cancer and precancerous tissue with cell invasion.Methods:Cervical cancer tissue, precancerous tissue and normal cervical tissue surgically removed in our hospital between May 2013 and April 2015 were collected;immunohistochemical staining kits were used to detect the positive protein expression rate of Twist and YB-1 gene;fluorescence quantitative PCR kits were used to detect Twist, YB-1 and invasion gene mRNA expression.Results:Twist and YB-1 mRNA expression and positive protein expression rate as well as USP22, Rab11, Rac1 and ANXA5 mRNA expression in cervical cancer tissue and precancerous tissue were significantly higher than those in normal cervical tissue, Twist and YB-1 mRNA expression and positive protein expression rate as well as USP22, Rab11, Rac1 and ANXA5 mRNA expression in cervical cancer tissue were significantly higher than those in precancerous tissue;USP22, Rab11, Rac1 and ANXA5 mRNA expression in cervical cancer tissue and precancerous tissue with positive Twist and YB-1 expression were significantly higher than those in cervical cancer tissue and precancerous tissue with negative Twist and YB-1 expression.Conclusion:Highly expressed Twist and YB-1 in cervical cancer and precancerous tissue can promote cell invasion.

Effect of Twist, Snail and YB-1 gene expression in cervical cancer tissue on cell invasion and epithelial-mesenchymal transition

Objective:To study the effect of Twist, Snail and YB-1 gene expression in cervical cancer tissue on cell invasion and epithelial-mesenchymal transition. Methods:Cervical cancer tissue samples and tissue samples adjacent to carcinoma were collected from 138 patients with radical operation for cervical cancer, fluorescence quantitative PCR method was used to detect the mRNA expression of Twist, Snail and YB-1 genes, cell invasion-related genes and epithelial-mesenchymal transition marker genes, the Pearson test was used to analyze the correlation of Twist, Snail and YB-1 gene mRNA expression in cervical cancer tissue with cell invasion and epithelial-mesenchymal transition.Results:Twist, Snail and YB-1 gene mRNA expression in cervical cancer tissue were higher than those in tissue adjacent to carcinoma, the invasion genes STAT3, YAP1, TUG1, FoxM1 and Rab11 mRNA expression were higher than those in tissue adjacent to carcinoma, and the epithelial-mesenchymal transition markers E-cadherin andβ-catenin gene mRNA expression were lower than those in tissue adjacent to carcinoma while vimentin gene mRNA expression was higher than that in tissue adjacent to carcinoma. Pearson test showed that Twist, Snail and YB-1 gene mRNA expression in cervical cancer tissue were directly correlated with cell invasion and epithelial-mesenchymal transition. Conclusion:Twist, Snail and YB-1 genes are highly expressed in cervical cancer tissue, and their abnormal expression directly leads to the increased tumor cell invasion activity and the aggravated epithelial-mesenchymal transition.

Correlation of serum bFGF and CXCL16 levels with cancer cell growth and invasion in patients with cervical cancer

Objective: To investigate the correlation of serum bFGF and CXCL16 levels with cancer cell growth and invasion in patients with cervical cancer. Methods: A total of 119 patients who were diagnosed with cervical cancer in Panyu District Shiqiao Hospital and Guangzhou First People's Hospital between October 2013 and October 2016 were collected as observation group, and 100 patients with cervical polyp (benign) who were treated in the hospitals during the same period were selected as control group. bFGF and CXCL16 levels in serum as well as proliferation and invasion gene expression in cervical lesions were detected, and Pearson test was used to further evaluate the correlation of serum bFGF and CXCL16 levels with cancer cell growth and invasion in patients with cervical cancer. Results: Serum bFGF and CXCL16 levels of observation group were higher than those of control group, and LATS1, SFRP2 and TET1 mRNA expression in cervical tissue were significantly lower than those of control group while HERC4, HMGB-1, HIF-1α, YAP1, Twist and RbAp48 mRNA expression were higher than those of control group;serum bFGF and CXCL16 levels in patients with cervical cancer were negatively correlated with LATS1, SFRP2 and TET1 mRNA expression in cervical tissue, and positively correlated with HERC4, HMGB-1, HIF-1α, YAP1, Twist and RbAp48 mRNA expression. Conclusion: Serum bFGF and CXCL16 levels abnormally increase in patients with cervical cancer, and they are directly correlated with the proliferation and invasion activity of cancer cells.

Expression and Biological Function of N-myc Down-regulated Gene 1 in Human Cervical Cancer

The expression of N-myc down-regulated gene 1 (NDRG1) has previously been reported to be involved in the proliferation,differentiation,invasion and metastasis of cancer cells,but its role in cervical cancer is still unclear.This study aimed to investigate the expression of NDRG1gene in human cervical cancer and its effect on aggressive tumor behaviors.The NDRG1 expression in cervical tissues and cells was detected by RT-PCR.Specific expression plasmid pEGFP-N1-NDRG1-GFP was used to enhance the expression of NDRG1 in human cervical cancer cell lines.The mRNA and protein level of NDRG1 was assessed by RT-PCR and Western blotting,respectively.Its effects on cell proliferation,migration,invasion,cell cycle and apoptosis were detected by MTT,transwell migration assay and flow cytometry (FCM),respectively.The results showed that the expression of NDRG1 in cervical cancer tissues and cells was significantly lower than in normal cervical tissues (P【0.001).After transfection with pEGFP-N1-NDRG1-GFP,the mRNA and protein expression of NDRG1 was up-regulated in Siha cells,which suppressed cell proliferation (P【0.001),induced cell cycle arrest (P【0.05),reduced invasion and migration of Siha cells (P【0.05),but caused no cell apoptosis.Moreover,vascular endothelial growth factor (VEGF),a tumor-induced angiogenesis factor,was markedly reduced and E-cadherin,a cell adhesion molecule,was increased in the cells transfected with pEGFP-N1-NDRG1-GFP.It was concluded that up-regulated NDRG1 may play a role in the suppression of malignant cell growth,invasion and metastasis of human cervical cancer.

Improved Detection of Cervical Cancer and High Grade Neoplastic Lesions by a Combination of Conventional Cytology and DNA Automated Image Cytometer

OBJECTIVE: To reduce false-negative rates of population based cervical screening programs employing conventional cytology in combination with automated DNA image cytometer. METHODS: Involved cervical samples from a total of 3603 women were taken by a cervix brush and then placed into a fixative solution. The cells were separated from mucus by mechanical and chemical treatment after which they were deposited onto microscope slides by a cytospin. Two slides were prepared from each case;one slide was stained by Papanicolaou stain for conventional cytology examination, while the other slide was stained by a DNA specific and stoichiometric stain. The latter slide was used to determine the relative amount of DNA in the cell nuclei in order to assess the ploidy status of the epithelial cells. Enrolled in the study, 157 women were followed by colposcopy examination where punch biopsies were taken from the visible lesions or from suspicious areas. The results of the conventional cytology were then compared to the DNA image cytometer for all samples. RESULTS: Histopathology diagnosed 51 lesions from the 132 biopsied cases as CIN2 or higher, including 27 CIN2, 16 CIN3 and 8 invasive cancers. Conventional cytology correctly identified 29 of the 51 high grade CIN and in-vasive cancer, while DNA image cytometer correctly identified 38 high grade CIN and invasive cancer using the crite-rion that at least three cells were found on the slide that contained DNA amount in excess of 5c. 42 out of 51 high grade CIN and invasive cancer were found by conventional cytology in combination with DNA image cytometer. Sensitivities were 56.8%, 74.5% and 82.4%, while specificities were 86.2%, 81.5% and 81.5% in conventional cytology, DNA image cytometer and combination both cytology and DNA image cytometer respectively. CONCLUSION: The study demon-strated that screening for high grade neoplastic lesions and cervical cancer by DNA image cytometer or combination of conventional cytology and DNA image cytometer is more sensitive than conventional screening approach.

Correlation between the ultrasonic elastography parameters of cervical cancer and the malignant biological behavior of cancer cells

Objective:To investigate the correlation between the ultrasonic elastography parameters of cervical cancer and the malignant biological behavior of cancer cells.Methods: A total of 180 patients with cervical cancer who were diagnosed and treated with radical operation in this hospital were selected as research objects, and the cervical cancer tissue and adjacent tissue samples were collected during operation and stored in liquid nitrogen tanks. The ultrasonic elastography strain ratio of cervical cancer and adjacent tissues was detected before operation, and the differences in the expression of genes associated with proliferation, invasion and autophagy were compared. Pearson test was used to evaluate the correlation between the cervical cancer strain ratio and the malignant biological behavior of cancer cells. Results: The strain ratio level of cervical cancer tissue was higher than that of adjacent tissue;expressions of proliferation-related genes AEG-1, TRAF6 and FoxM1 mRNA were higher than those in adjacent tissue whereas expressions of SFRP2, MST1 and Bmi-1 mRNA were lower than those in adjacent tissue;expressions of invasion-related genes ILK, Sox2, Twist, YB-1 and Rab11 mRNA were higher than those in adjacent tissue;expressions of autophagy-related genes ARHI, Beclin1, PULK and PI3KC3 mRNA were lower than those in adjacent tissue.Conclusions: Ultrasonic strain ratio level of cervical cancer is closely related to the proliferation, invasion and autophagy activity of cancer cells, and is positively correlated with the overall malignancy of the tumor.

Changes of PI3K/AKT/mTOR signaling pathway in the progression of cervical cancer and its target genes

Objective: To study the changes of PI3K/AKT/mTOR signaling pathway in the progression of cervical cancer and explore its target genes. Methods: The patients who underwent surgical resection and were diagnosed with cervical cancer by postoperative pathology in the First People's Hospital of Jiangxia District Wuhan City between June 2014 and December 2017 were selected, and the cervical cancer lesion tissues and lesion tissues adjacent to cervical cancer were kept;patients who underwent conization and were diagnosed with cervical intraepithelial neoplasia by postoperative pathology in the First People's Hospital of Jiangxia District Wuhan City during the same period were selected, and the cervical intraepithelial neoplasia tissues were kept. The protein levels of PI3K/AKT/mTOR signaling molecules as well as the mRNA expression of proliferation genes and invasion genes were determined. Results: p-PI3K, p-AKT, mTOR, p70S6K and p-4EBP1 protein levels as well as CyclinD1, Survivin, Piwil2, RACK1, EFEMP1 and VEGF mRNA expression in cervical cancer lesion tissues were significantly higher than those in adjacent lesion tissues and cervical intraepithelial neoplasia tissues whereas THBS2, Beclin1, E-cadherin, TIMP1 and TIMP2 mRNA expression were significantly lower than those in adjacent lesion tissues and cervical intraepithelial neoplasia tissues;p-PI3K, p-AKT, mTOR, p70S6K and p-4EBP1 protein levels in cervical cancer lesion tissues were positively correlated with CyclinD1, Survivin, Piwil2, RACK1, EFEMP1 and VEGF mRNA expression, and negatively correlated with THBS2, Beclin1, E-cadherin, TIMP1 and TIMP2 mRNA expression. Conclusion: Excessive activation of PI3K/AKT/mTOR signaling pathway during the progression of cervical cancer can change the expression of multiple proliferation and invasion genes to promote the proliferation and invasion of cancer cells.

Changes in three-dimensional power Doppler ultrasound features before and after neoadjuvant chemotherapy for cervical cancer and their relationship with malignant molecule expression

Objective:To study the changes in three-dimensional power Doppler ultrasound features before and after neoadjuvant chemotherapy for cervical cancer and their relationship with malignant molecule expression.Methods: The patients who were diagnosed with cervical cancer and received neoadjuvant chemotherapy in Wuhan Red Cross Hospital between March 2015 and October 2017 were selected as the cervical cancer group, and the patients who received cervical biopsy and were diagnosed with stage I cervical intraepithelial neoplasia by pathological findings during the same period were selected as the control group. Before biopsy, three-dimensional power Doppler ultrasonography was performed to measure VI, FI and VFI;the tissues of cervical cancer group before and after chemotherapy as well as the biopsy tissues of control group were collected to measure the expression of proliferation genes, invasion genes and angiogenesis genes.Results: The VI, FI and VFI levels as well as the Piwil2 gene (Piwil2), CyclinD1, N-Myc downstream regulated gene 3 (NDRG3), CXC chemokine ligand 5 (CXCL5), cathepsin-L (CAT-L), EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), angiotensin (Ang)1, Ang2 and angiopoietin-like protein 4 (ANGPTL4) mRNA expression levels in the tissues of cervical cancer group before and after chemotherapy were all significantly higher than those of control group whereas the thrombospondin 2 (THBS2), Smac gene (Smac), large tumor suppressor kinase 1 (LATS1), reversion-inducing-cysteine-rich protein with kazal motifs (RECK) and plas minogen activator inhibitor 1 (PAI-1) mRNA expression levels in the tissues were all significantly lower than those of control group, and the VI, FI and VFI levels as well as the Piwil2, CyclinD1, NDRG3, CXCL5, CAT-L, EFEMP1, Ang1, Ang2 and ANGPTL4 mRNA expression levels in the tissues of cervical cancer group after chemotherapy were all significantly lower than those before chemotherapy whereas the THBS2, Smac, LATS1, RECK and PAI-1 mRNA expression levels were all significantly higher than those before chemotherapy;the VI, FI and VFI levels in cervical cancer tissues were positively correlated with the Piwil2, CyclinD1, NDRG3, CXCL5, CAT-L, EFEMP1, Ang1, Ang2 and ANGPTL4 mRNA expression levels, and negatively correlated with the THBS2, Smac, LATS1, RECK and PAI-1 mRNA expression levels.Conclusion: The changes in the three-dimensional power Doppler ultrasound parameters before and after neoadjuvant chemotherapy for cervical cancer can reflect the changes in proliferation, invasion and angiogenesis gene expression in the lesions.

Effects of preoperative neoadjuvant chemotherapy on malignant biological characteristics in the lesions of patients with ⅠB-ⅡB cervical cancer

Objective: To investigate the effects of preoperative neoadjuvant chemotherapy on malignant biological characteristics in the lesions of patients with ⅠB-ⅡB cervical cancer. Methods:Patients who were diagnosed with ⅠB-ⅡB cervical cancer and underwent surgical treatment in Navy General Hospital of PLA between February 2015 and May 2017 were selected as the research subjects and randomly divided into the neoadjuvant chemotherapy group who underwent preoperative neoadjuvant chemotherapy and the control group who underwent routine preoperative preparation. After surgical resection, the mRNA expression of tumor suppressor genes, proliferation genes as well as migration and invasion genes in cervical cancer tissue were determined by fluorescence quantitative PCR. Results: After surgical resection, tumor suppressor genes THBS2, PTEN, LAST1 and eIF4E3 mRNA expression in cervical cancer tissue of adjuvant chemotherapy group were significantly higher than those of routine surgery group whereas proliferation genes RUNX2, CyclinD1, ALEX1, ALDH1 and ABCG2 as well as migration and invasion genes CXCL12, CXCR4, MMP9, S100A6 and N-cadherin mRNA expression were significantly lower than those of routine surgery group. Conclusion:Preoperative neoadjuvant chemotherapy can inhibit the proliferation, migration and invasion of cancer cells within the lesions of patients with ⅠB-ⅡB cervical cancer.

Effect of Zhenwu decoction combined with paclitaxel neoadjuvant chemotherapy on the malignant degree of cervical cancer

Objective:To study the effect of Zhenwu decoction combined with paclitaxel neoadjuvant chemotherapy on the malignant degree of cervical cancer.Methods: Patients with cervical cancer who accepted neoadjuvant chemotherapy in Nanchong Central Hospital and Suining Central Hospital between March 2015 and June 2017 were selected and randomly divided into two groups, the observation group received neoadjuvant chemotherapy combined with Zhenwu decoction therapy, and the control group received neoadjuvant chemotherapy. Before chemotherapy and after 2 courses of chemotherapy, the serum was collected to detect the contents of tumor markers;after chemotherapy, the surgically removed cervical cancer lesion was collected to detect the mRNA expression of proliferation genes, tumor suppressor genes and invasion genes.Results: After 2 course of chemotherapy, CA12-5, bFGF and SCC-Ag levels in serum of both groups were lower than those before treatment, and CA12-5, bFGF and SCC-Ag levels in serum as well as Ki-67, TK-1, SOX-2, CyclinD1, S100A6, N-cadherin, Gal-3, CatL and CatD mRNA expression in cervical cancer lesion of observation group were lower than those of control group whereas p53, p16, FHIT, NF2-1 and LATS1 mRNA expression in cervical cancer lesions were higher than those of control group.Conclusion:Zhenwu decoction combined with paclitaxel neoadjuvant chemotherapy can be more effective than neoadjuvant chemotherapy alone to kill cervical cancer cells and reduce the malignancy of cervical cancer.

Effect of preoperative hyperselective uterine arterial chemoembolization on the infiltrative growth of cancer cells in locally advanced cervical cancer

Objective:To study the effect of preoperative hyperselective uterine arterial chemoembolization on the infiltrative growth of cancer cells in locally advanced cervical cancer.Methods: Patients with locally advanced cervical cancer who underwent surgical resection in Ankang Municipality Maternity and Child Care between February 2015 and October 2017 were selected and randomly divided into the observation group who received preoperative hyperselective uterine arterial chemoembolization and the control group who received routine preoperative preparation. The contents of tumor markers in serum were determined at diagnosis and 1 day before undergoing surgery;the expression levels of tumor suppressor genes and invasion genes were determined after surgical resection.Results: 1 day before undergoing surgery, serum CA125, TSGF, SCC and HE4 levels of observation group were lower than those at diagnosis whereas serum CA125, TSGF, SCC and HE4 levels of control group were not significant different from those at diagnosis;after surgical resection, RASSF2A, FHIT, eIF4E3, RIZ1, DAPK and Syk protein expression in cervical cancer lesions of observation group were significantly higher than those of control group;whereas RbAp48, Vimentin, N-cadherin, Sox2,β-catenin and MMP9 protein expression were significantly lower than those of control group.Conclusion: Preoperative hyperselective uterine arterial chemoembolization can inhibit the infiltrative growth of cancer cells in locally advanced cervical cancer.

Value of serum sE-cadherin and CXCL16 contents for the evaluation of malignant biological behaviors in cervical cancer

Objective: To study the value of serum sE-cadherin and CXCL16 contents for the evaluation of malignant biological behaviors in cervical cancer. Methods: Patients with cervical cancer who accepted surgical treatment in Huanggang Central Hospital between August 2014 and April 2017 were selected as the cervical cancer group of the research and healthy subjects who received physical examination in Huanggang Central Hospital over the same period were selected as the control group. The contents of sE-cadherin and CXCL16 in the serum of cervical cancer group and control group as well as the mRNA expression of proliferation genes and invasion genes in cervical cancer lesion and adjacent lesion of cervical cancer group were determined. Results: Serum sE-cadherin content of cervical cancer group was greatly lower than that of control group whereas CXCL16 content was greatly higher than that of control group;SP2, SND1, ANGPTL4, S6K1, Rab11, S100A6, N-cadherin and MMP9 mRNA expression in cervical cancer lesion were greatly higher than those in adjacent lesion, negatively correlated with serum sE-cadherin content, and positively correlated with serum CXCL16 content whereas TET1, HSG and FHIT mRNA expression were greatly lower than those in adjacent lesion, positively correlated with serum sE-cadherin content, and negatively correlated with serum CXCL16 content. Conclusion: The decreased sE-cadherin and increased CXCL16 in serum of patients with cervical cancer can assess the malignant biological behaviors of proliferation and invasion in the lesion.

Effect of Tegafur Gimeracil Oteracil Potassium Capsule + Kangai injection + intensity-modulated radiation therapy on the cellular malignant biological processes in advanced cervical cancer lesion

Objective:To study the effect of Tegafur Gimeracil Oteracil Potassium Capsule + Kangai injection + intensity-modulated radiation therapy on the cellular malignant biological processes in advanced cervical cancer lesion.Methods: Patients who were diagnosed with advanced cervical cancer in the Second People Hospital of Banan District Chongqing between April 2015 and March 2017 were selected and divided into two groups, group A received Tegafur Gimeracil Oteracil Potassium Capsule + Kangai injection + intensity-modulated radiation therapy, and group B received cisplatin + intensity-modulated radiation therapy. Serum contents of tumor markers, tumor invasion molecules and tumor proliferation molecules of two groups of patients were detected before treatment as well as 2 weeks and 4 weeks after treatment.Results: Serum E-cad, STMN1, Fas and p53 levels of both groups of patients 2 weeks and 4 weeks after treatment were significantly higher than those before treatment while TSGF, TK1, SCC-Ag, CA125, OPN, MMP9, NGAL, CyclinE, CyclinD1 and PCNA levels were significantly lower than those before treatment, and serum E-cad, STMN1, Fas and p53 levels of group A 2 weeks and 4 weeks after treatment were significantly higher than those of group B while TSGF, TK1, SCC-Ag, CA125, OPN, MMP9, NGAL, CyclinE, CyclinD1 and PCNA levels were significantly lower than those of group B.Conclusion: Tegafur Gimeracil Oteracil Potassium Capsule + Kangai injection + intensity-modulated radiation therapy for advanced cervical cancer can induce cancer cell apoptosis and inhibit cancer cell proliferation and invasion.

Serum Spondin-2 expression,tumor invasion,and antitumor immune response in patients with cervical cancer

BACKGROUND Cervical cancer is a gynecological malignancy common in middle-aged and older patients,with a high mortality rate.Spondin-2 is an extracellular matrix protein that involved in innate and acquired immune responses.Herein,we investigated the relationship between serum Spondin-2 expression,tumor invasion and infiltration,and immune response in patients with cervical cancer and provided a theoretical basis for clinical practice.AIM To investigate the relationship between serum Spondin-2 expression and cervical cancer-related indicators.METHODS Overall,147 patients with cervical cancer who were admitted to our institution between January 2019 and August 2019 were assigned to the cervical cancer group,and 92 patients with benign uterine lesions and 86 healthy individuals were assigned to the benign and control groups,respectively.In each group,serum Spondin-2 expression was measured,and the receiver operating characteristic(ROC)curve was determined.Patients with cervical cancer were classified into high or low Spondin-2 groups depending on the Spondin-2 threshold value used for diagnosing cervical cancer.Patient’s clinical data were collected to compare the clinicopathologic characteristics,immune cytokine levels,and prognosis of patients with varying Spondin-2 expression levels.RESULTS The expression level of serum Spondin-2 was significantly higher in the cervical cancer group than in the benign and control groups(P<0.05).According to the ROC curve,the cutoff value of Spondin-2 used in the diagnosis of cervical carcinoma was 25.68±7.11μg/L.The proportion of patients with Federation of Gynecology and Obstetrics stage III,nerve invasion,vascular invasion,and lymph node metastasis was higher in the high Spondin-2 group than in the low Spondin-2 group(P<0.05).Interleukin-5(IL-5)and IL-4 Levels were higher in the high Spondin-2 group than in the low Spondin-2 group.In contrast,IL-2 and tumor necrosis factor-αlevels were lower in the high Spondin-2 group than in the low Spondin-2 group(P<0.05).After 3 years of follow-up,progression-free survival and overall survival were significantly shorter in the high Spondin-2 group than in the low Spondin-2 group(P<0.05).CONCLUSION The expression of serum Spondin-2 is upregulated in patients with cervical carcinoma and is related to tumor invasion and infiltration,antitumor immune response,and prognosis.