以人血清载脂蛋白AⅡ为抗原,免疫BALB/C小鼠,将脾细胞与NS-1骨髓瘤细胞融合,经HAT选择性培养,特异性筛选及克隆化,获得1-C1、2-C2两株分泌抗人血清载脂蛋白AⅡ单克隆抗体杂交瘤细胞株。免疫双扩散确定它们分泌抗体的亚型均为IgG1;吸收...以人血清载脂蛋白AⅡ为抗原,免疫BALB/C小鼠,将脾细胞与NS-1骨髓瘤细胞融合,经HAT选择性培养,特异性筛选及克隆化,获得1-C1、2-C2两株分泌抗人血清载脂蛋白AⅡ单克隆抗体杂交瘤细胞株。免疫双扩散确定它们分泌抗体的亚型均为IgG1;吸收试验及ELISA交叉试验表明单克隆抗体的特异性;ELISA测定其培养上清中抗体效价都为1:100,诱生腹水效价分别为1:10000和1:5000;两株杂交瘤细胞染色体均数分别为96.34±6.06、95.01±5.38;采用Protein A Sepharose 4B层析柱纯化了1-C1杂交瘤细胞培养上清中的单克隆抗体。展开更多
To evaluate the effects of left ventricular contractility on the changes of aver age image intensity (AII) of the myocardial integrated backscatter (IB) and cyclic variation in IB (CVIB), 7 adult mongrel dogs were stu...To evaluate the effects of left ventricular contractility on the changes of aver age image intensity (AII) of the myocardial integrated backscatter (IB) and cyclic variation in IB (CVIB), 7 adult mongrel dogs were studied. The magnitude of AII and CVIB were measured from myocardial IB carves before and after dobuta mine or propranolol infusion. Dobutamine or propranolol did not affect the magnitude of AII (13.8±0.7 vs 14.7±0.5, P >0.05 or 14.3±0.5 vs 14.2±0.4, P >0.05). However, dobutamine produced a significant increase in the magnitude of CVIB (6.8±0.3 vs 9.5±0.6, P <0.001) and propranolol induced significant decrease in the magnitude of CVIB (7.1±0.2 vs 5.2±0.3, P <0.001). The changes of the magnitude of AII and CVIB in the myocardium have been demonstrated to reflect different myocardial physiological and pathological changes respectively. The alteration of contractility did not affect the magnitude of AII but induced significant change in CVIB. The increase of left ventricular contractility res ulted in a significant rise of the magnitude of CVIB and the decrease of left ventricular contractility resulted in a significant fall of the magnitude of CVIB.展开更多
文摘以人血清载脂蛋白AⅡ为抗原,免疫BALB/C小鼠,将脾细胞与NS-1骨髓瘤细胞融合,经HAT选择性培养,特异性筛选及克隆化,获得1-C1、2-C2两株分泌抗人血清载脂蛋白AⅡ单克隆抗体杂交瘤细胞株。免疫双扩散确定它们分泌抗体的亚型均为IgG1;吸收试验及ELISA交叉试验表明单克隆抗体的特异性;ELISA测定其培养上清中抗体效价都为1:100,诱生腹水效价分别为1:10000和1:5000;两株杂交瘤细胞染色体均数分别为96.34±6.06、95.01±5.38;采用Protein A Sepharose 4B层析柱纯化了1-C1杂交瘤细胞培养上清中的单克隆抗体。
文摘To evaluate the effects of left ventricular contractility on the changes of aver age image intensity (AII) of the myocardial integrated backscatter (IB) and cyclic variation in IB (CVIB), 7 adult mongrel dogs were studied. The magnitude of AII and CVIB were measured from myocardial IB carves before and after dobuta mine or propranolol infusion. Dobutamine or propranolol did not affect the magnitude of AII (13.8±0.7 vs 14.7±0.5, P >0.05 or 14.3±0.5 vs 14.2±0.4, P >0.05). However, dobutamine produced a significant increase in the magnitude of CVIB (6.8±0.3 vs 9.5±0.6, P <0.001) and propranolol induced significant decrease in the magnitude of CVIB (7.1±0.2 vs 5.2±0.3, P <0.001). The changes of the magnitude of AII and CVIB in the myocardium have been demonstrated to reflect different myocardial physiological and pathological changes respectively. The alteration of contractility did not affect the magnitude of AII but induced significant change in CVIB. The increase of left ventricular contractility res ulted in a significant rise of the magnitude of CVIB and the decrease of left ventricular contractility resulted in a significant fall of the magnitude of CVIB.
