利用含有苜蓿花叶病毒中国分离株 (AIMV - Ch)复制酶 (P2 亚基 )基因编码区 5’端 c DNA (130 6 bp)的重组质粒 p AM5和编区 3’端及其非编码区 (12 34bp的重组质粒 p AM3构建了含有复制 (P2 亚基 )基因全长 c DNA及其 3’端非编码区的...利用含有苜蓿花叶病毒中国分离株 (AIMV - Ch)复制酶 (P2 亚基 )基因编码区 5’端 c DNA (130 6 bp)的重组质粒 p AM5和编区 3’端及其非编码区 (12 34bp的重组质粒 p AM3构建了含有复制 (P2 亚基 )基因全长 c DNA及其 3’端非编码区的重组质粒 p AM35 ,并将其重组于植物表达载体 p GA6 43,构建其正链 RNA的表达载体 p GA35 。展开更多
To compare the differences between proliferation and cytotoxicity of adherent natural killer （A-NK） cells cultured with serum-free medium AIMV and standard serum-containing medium in vitro, and also observe the assi...To compare the differences between proliferation and cytotoxicity of adherent natural killer （A-NK） cells cultured with serum-free medium AIMV and standard serum-containing medium in vitro, and also observe the assisting effect of IL-12 on the activation and the morphology character of IL-2-treated A-NK cells, cellular proliferation was evaluated by MTT method in vitro. The morphology of the target cells killed by A-NK cells was observed through electroscope. All of the A-NK cells cultured in serum-free medium AIMV could rapidly proliferate and keep high cytotoxicity compared with that in standard serum-containing medium. A-NK cells activated by both moderate-dose IL-2 and IL-12 were superior to the high-dose IL-2-treated A-NK cells. These data indicated that serum-free medium AIMV could replace standard serum-containing medium for culturing A-NK cells, and moderate-dose IL-2 and IL-12 could reduce side effects caused by high-dose IL-2. The study provided a new experimental basis for experimental and clinical preparation of A-NK cells. Cellular ＆ Molecular Immunology.展开更多
文摘利用含有苜蓿花叶病毒中国分离株 (AIMV - Ch)复制酶 (P2 亚基 )基因编码区 5’端 c DNA (130 6 bp)的重组质粒 p AM5和编区 3’端及其非编码区 (12 34bp的重组质粒 p AM3构建了含有复制 (P2 亚基 )基因全长 c DNA及其 3’端非编码区的重组质粒 p AM35 ,并将其重组于植物表达载体 p GA6 43,构建其正链 RNA的表达载体 p GA35 。
文摘To compare the differences between proliferation and cytotoxicity of adherent natural killer （A-NK） cells cultured with serum-free medium AIMV and standard serum-containing medium in vitro, and also observe the assisting effect of IL-12 on the activation and the morphology character of IL-2-treated A-NK cells, cellular proliferation was evaluated by MTT method in vitro. The morphology of the target cells killed by A-NK cells was observed through electroscope. All of the A-NK cells cultured in serum-free medium AIMV could rapidly proliferate and keep high cytotoxicity compared with that in standard serum-containing medium. A-NK cells activated by both moderate-dose IL-2 and IL-12 were superior to the high-dose IL-2-treated A-NK cells. These data indicated that serum-free medium AIMV could replace standard serum-containing medium for culturing A-NK cells, and moderate-dose IL-2 and IL-12 could reduce side effects caused by high-dose IL-2. The study provided a new experimental basis for experimental and clinical preparation of A-NK cells. Cellular ＆ Molecular Immunology.
