Background: Protein kinase B (AKT/PKB) family is frequently amplified in ovarian cancer (OC). To the greatest of our knowledge, there is a lack of published reports about the amplification of the genes belonging to th...Background: Protein kinase B (AKT/PKB) family is frequently amplified in ovarian cancer (OC). To the greatest of our knowledge, there is a lack of published reports about the amplification of the genes belonging to the AKT family among Sudanese women with OC. The present study was conducted to detect the AKT1 gene amplification and its association with tumour types, grades, and ages among Sudanese women with OC, bearing in mind the ethnic variation. Methods: This institution-based study included 79 cases of women diagnosed with ovarian cancer (OC) at Omdurman Maternity Hospital in the period 2013-2018. Formalin-fixed, paraffin-embedded (FFPE) tissue sections were used to extract RNA. AKT1 gene amplification was assessed using quantitative real-time PCR. Results: The mean age (±SD) of included women was 49.29 (±13.612). The amplification of AKT1 gene was observed in 18/79 (22.8%) of OC women, with a high frequency in women with undifferentiated 1/2 (50%), clear cell 2/6 (33.3%), mucinous 3/11 (27.3%), endometrioid 3/17 (17.6%), and serous carcinomas 5/30 OC (16.7%). High frequency was seen in women with low (26.3%;n = 10/28) rather than in higher (19.5%;n = 8/33) grade carcinoma, and in older (25.8%;n = 8/23) rather than younger (18.2%;n = 2/9) women. No significant association between AKT1 gene amplification and tumour types, grades, and ages of women was observed (Fisher’s Exact test: p = 0.405, 0.593 and 0.851, respectively). Conclusion: AKT1 gene amplification arises in around one-fifth of Sudanese women with ovarian cancer (OC). It is seen more in undifferentiated, clear cell, and mucinous tumours types, and more frequently in low tumour grade and older women, but not to a statistically significant level. These outcomes sustenance previous studies suggesting that activated AKT genes have a vital role in OC progression and may offer a plan for targeted therapy and prognostic evaluation.展开更多
目的研究微小RNA-34a-5p(miR-34a-5p)靶向AKT1基因对子宫内膜异位症(EM)子宫内膜基质细胞(ESCs)侵袭及自噬的调控作用。方法原代分离、培养EM患者ESCs,构建negative control RNA(NC)及mimic细胞模型,采用双荧光肾素酶报告系统验证miR-34...目的研究微小RNA-34a-5p(miR-34a-5p)靶向AKT1基因对子宫内膜异位症(EM)子宫内膜基质细胞(ESCs)侵袭及自噬的调控作用。方法原代分离、培养EM患者ESCs,构建negative control RNA(NC)及mimic细胞模型,采用双荧光肾素酶报告系统验证miR-34a-5p与AKT1基因存在结合位点,RT-PCR及Western blotting检测并验证2组细胞miR-34a-5p和AKT1基因的表达及调控关系;检测miR-34a-5p对ESCs的增殖、迁移、侵袭、凋亡的影响。结果EM患者ESCs中miR-34a-5p较非EM患者明显降低,而AKT1基因的表达明显升高,且两者变化呈负相关;miR-34a-5p通过特异性结合AKT1基因的3’UTR区对AKT1基因的表达进行调控;转染miR-34a-5p mimic可使ESCs中miR-34a-5p表达升高,AKT1基因表达降低,同时使ESCs的增殖、迁移及侵袭能力增强,而凋亡及自噬能力下降。结论miR-34a-5p靶向AKT1基因促进ESCs的增殖、迁移及侵袭能力,降低其凋亡及自噬能力,可能在EM的发病过程中发挥作用。展开更多
文摘Background: Protein kinase B (AKT/PKB) family is frequently amplified in ovarian cancer (OC). To the greatest of our knowledge, there is a lack of published reports about the amplification of the genes belonging to the AKT family among Sudanese women with OC. The present study was conducted to detect the AKT1 gene amplification and its association with tumour types, grades, and ages among Sudanese women with OC, bearing in mind the ethnic variation. Methods: This institution-based study included 79 cases of women diagnosed with ovarian cancer (OC) at Omdurman Maternity Hospital in the period 2013-2018. Formalin-fixed, paraffin-embedded (FFPE) tissue sections were used to extract RNA. AKT1 gene amplification was assessed using quantitative real-time PCR. Results: The mean age (±SD) of included women was 49.29 (±13.612). The amplification of AKT1 gene was observed in 18/79 (22.8%) of OC women, with a high frequency in women with undifferentiated 1/2 (50%), clear cell 2/6 (33.3%), mucinous 3/11 (27.3%), endometrioid 3/17 (17.6%), and serous carcinomas 5/30 OC (16.7%). High frequency was seen in women with low (26.3%;n = 10/28) rather than in higher (19.5%;n = 8/33) grade carcinoma, and in older (25.8%;n = 8/23) rather than younger (18.2%;n = 2/9) women. No significant association between AKT1 gene amplification and tumour types, grades, and ages of women was observed (Fisher’s Exact test: p = 0.405, 0.593 and 0.851, respectively). Conclusion: AKT1 gene amplification arises in around one-fifth of Sudanese women with ovarian cancer (OC). It is seen more in undifferentiated, clear cell, and mucinous tumours types, and more frequently in low tumour grade and older women, but not to a statistically significant level. These outcomes sustenance previous studies suggesting that activated AKT genes have a vital role in OC progression and may offer a plan for targeted therapy and prognostic evaluation.
文摘目的研究微小RNA-34a-5p(miR-34a-5p)靶向AKT1基因对子宫内膜异位症(EM)子宫内膜基质细胞(ESCs)侵袭及自噬的调控作用。方法原代分离、培养EM患者ESCs,构建negative control RNA(NC)及mimic细胞模型,采用双荧光肾素酶报告系统验证miR-34a-5p与AKT1基因存在结合位点,RT-PCR及Western blotting检测并验证2组细胞miR-34a-5p和AKT1基因的表达及调控关系;检测miR-34a-5p对ESCs的增殖、迁移、侵袭、凋亡的影响。结果EM患者ESCs中miR-34a-5p较非EM患者明显降低,而AKT1基因的表达明显升高,且两者变化呈负相关;miR-34a-5p通过特异性结合AKT1基因的3’UTR区对AKT1基因的表达进行调控;转染miR-34a-5p mimic可使ESCs中miR-34a-5p表达升高,AKT1基因表达降低,同时使ESCs的增殖、迁移及侵袭能力增强,而凋亡及自噬能力下降。结论miR-34a-5p靶向AKT1基因促进ESCs的增殖、迁移及侵袭能力,降低其凋亡及自噬能力,可能在EM的发病过程中发挥作用。
