The most effective way to clarify the structure-function relationship of insulin at present is by the inference from the detailed analysis of more and less competent insulins and modified insulins.Analogues of insulin...The most effective way to clarify the structure-function relationship of insulin at present is by the inference from the detailed analysis of more and less competent insulins and modified insulins.Analogues of insulin with derivatized or exchanged Al-Glycine reveal the sensitivity of the biological activity to structural modifications of展开更多
We have determined the crystal structure of Al-(D-Trp) insulin and discovered that it belongs to the trigonal system with space group R3. The parameters of the unit cell are a=b=78.6, c=50.0. A set of data for half a ...We have determined the crystal structure of Al-(D-Trp) insulin and discovered that it belongs to the trigonal system with space group R3. The parameters of the unit cell are a=b=78.6, c=50.0. A set of data for half a sphere reciprocal space to a spacing of 2.2 were collected. The model was adjusted and refined by using a step-by-step approach and a stereochemically-restrained least squares program, assisted by manual revision based on the difference Fourier maps, to a final R-factor of 0.218. The main and side chains of both Al-D-Trp residues in the asymmetric unit are well ordered. The packing of Al-(D-Trp) insulin in the unit cell, the conformational differences with other insulin structures and its structure and function relationship bave also been discussed.展开更多
基金Project supported by Academia Sinicathe National Natural Science Foundation of China.
文摘The most effective way to clarify the structure-function relationship of insulin at present is by the inference from the detailed analysis of more and less competent insulins and modified insulins.Analogues of insulin with derivatized or exchanged Al-Glycine reveal the sensitivity of the biological activity to structural modifications of
文摘We have determined the crystal structure of Al-(D-Trp) insulin and discovered that it belongs to the trigonal system with space group R3. The parameters of the unit cell are a=b=78.6, c=50.0. A set of data for half a sphere reciprocal space to a spacing of 2.2 were collected. The model was adjusted and refined by using a step-by-step approach and a stereochemically-restrained least squares program, assisted by manual revision based on the difference Fourier maps, to a final R-factor of 0.218. The main and side chains of both Al-D-Trp residues in the asymmetric unit are well ordered. The packing of Al-(D-Trp) insulin in the unit cell, the conformational differences with other insulin structures and its structure and function relationship bave also been discussed.
