Loquat(Eriobotrya japonica Lindl.),a rare fruit native to China,has a long history of cultivation in China.Low temperature is the key factor restricting loquat growth and severely affects yield.Low temperature induces...Loquat(Eriobotrya japonica Lindl.),a rare fruit native to China,has a long history of cultivation in China.Low temperature is the key factor restricting loquat growth and severely affects yield.Low temperature induces the regeneration and metabolism of reduced glutathione(GSH)to alleviate stress damage via the participation of glu-tathione S-transferases(GSTs)in plants.In this study,16 GSTs were identified from the loquat genome according to their protein sequence similarity with Arabidopsis GSTs.On the basis of domain characteristics and phyloge-netic analysis of AtGSTs,these EjGSTs can be divided into 4 subclasses:Phi,Theta,Tau and Zeta.The basic prop-erties,subcellular localization,structures,motifs,chromosomal distribution and collinearity of the EjGST proteins or genes were further analyzed.Tandem and segmental gene duplications play pivotal roles in EjGST expansion.Cis-elements that respond to various hormones and stresses,especially those associated with low-temperature responsiveness,were predicted to be present in the promoters of EjGSTs.Moreover,analysis of gene expression profiles revealed that 9 of 16 EjGSTs may be involved in the low-temperature responsiveness of loquat leaves.In agriculture,5-aminolevulinic acid(ALA),a potential multifunctional plant growth regulator,can improve the stress response of plants.Among the 9 low-temperature-responsive EjGSTs,the expression of EjGSTU1 and EjGSTF1 significantly differed under cold stress in response to exogenous 5-aminolevulinic acid(ALA)pretreat-ment.The remarkable increase in GST activity and GSH/GSSG ratio reflected the increase in the cold response ability of loquat plants caused by exogenous ALA,thereby alleviating H2O2 accumulation and membrane lipid preoxidation.Overall,this study provides an initial exploration of the cold tolerance function of GSTs in loquat,offering a theoretical foundation for the development of cold-resistant loquat cultivars and new antifreeze agents.展开更多
The Alaskapox virus(AKPV)is a new Orthopoxvirus first identified in 2015 on a woman near Fairbanks,Alaska^([1]).This double-stranded DNA virus,part of the Poxviridae family,shares its genus with notable viruses like s...The Alaskapox virus(AKPV)is a new Orthopoxvirus first identified in 2015 on a woman near Fairbanks,Alaska^([1]).This double-stranded DNA virus,part of the Poxviridae family,shares its genus with notable viruses like smallpox,monkeypox,and cowpox.Despite these genetic ties,AKPV is distinct and potentially represents a new species within the Orthopoxvirus genus,as suggested by its sequenced genome^([2]).展开更多
为了更好地了解数字时代美国年轻人对图书馆和媒体的态度,揭示其行为特征,美国图书馆协会(American Library Association, ALA)以2022年的一项全国性调查为基础,于2023年11月1日发布了一份研究报告《Z世代和千禧一代:他们如何使用公共...为了更好地了解数字时代美国年轻人对图书馆和媒体的态度,揭示其行为特征,美国图书馆协会(American Library Association, ALA)以2022年的一项全国性调查为基础,于2023年11月1日发布了一份研究报告《Z世代和千禧一代:他们如何使用公共图书馆和经由媒体使用定义身份》(Gen Z and Millennials:How They Use Public Libraries and Identify Through Media Use)。这里的Z世代(Gen Z)是指2022年调查时13—25岁的人,千禧一代(Millennials)是指2022年调查时26—40岁的人。展开更多
以盆栽草莓为材料,研究了叶面喷布5-氨基乙酰丙酸(ALA)对草莓植株光合作用、叶绿素荧光特性、抗氧化酶活性和丙二醛(M DA)含量的影响.结果表明,100 m g/L ALA处理显著提高草莓叶片净光合速率(Pn),而且这一效应可能与其促进叶绿素含量和...以盆栽草莓为材料,研究了叶面喷布5-氨基乙酰丙酸(ALA)对草莓植株光合作用、叶绿素荧光特性、抗氧化酶活性和丙二醛(M DA)含量的影响.结果表明,100 m g/L ALA处理显著提高草莓叶片净光合速率(Pn),而且这一效应可能与其促进叶绿素含量和羧化效率(CE)提高,降低光呼吸速率(Rp)有关.叶绿素荧光动力学资料显示,ALA处理降低高光强(1 500μm o l.m-2.-s 1)下草莓叶片的初始荧光(Fo),表明它对光合膜系统有一定保护作用.ALA处理不仅明显提高草莓叶片最大荧光(Fm)和可变荧光(Fv),而且提高PSⅡ实际光化学效率(ΦPSⅡ)、光化学荧光猝灭(qP)、非光化学荧光猝灭系数(N PQ)、表观光合电子传递速率(ETR)、光化学速率(P CR)和天线热耗散(D),而降低了光下相对光合限制值(L(PFD)),表明叶绿素荧光产额提高和天线热耗散是保护光合器官并提高光合效率的两个方面.叶片抗氧化酶活性测定以及超氧化物歧化酶(SOD)活性抑制剂二乙基二硫代氨基甲酸(DDC)处理结果表明,ALA对草莓光合作用的促进作用还与其提高抗氧化酶活性有关.展开更多
基金This research was funded by grants from the Fujian Provincial Science and Technology Project(2021N5014,2022N5006)the Science and Technology Plan Project of Putian(2023GJGZ001).
文摘Loquat(Eriobotrya japonica Lindl.),a rare fruit native to China,has a long history of cultivation in China.Low temperature is the key factor restricting loquat growth and severely affects yield.Low temperature induces the regeneration and metabolism of reduced glutathione(GSH)to alleviate stress damage via the participation of glu-tathione S-transferases(GSTs)in plants.In this study,16 GSTs were identified from the loquat genome according to their protein sequence similarity with Arabidopsis GSTs.On the basis of domain characteristics and phyloge-netic analysis of AtGSTs,these EjGSTs can be divided into 4 subclasses:Phi,Theta,Tau and Zeta.The basic prop-erties,subcellular localization,structures,motifs,chromosomal distribution and collinearity of the EjGST proteins or genes were further analyzed.Tandem and segmental gene duplications play pivotal roles in EjGST expansion.Cis-elements that respond to various hormones and stresses,especially those associated with low-temperature responsiveness,were predicted to be present in the promoters of EjGSTs.Moreover,analysis of gene expression profiles revealed that 9 of 16 EjGSTs may be involved in the low-temperature responsiveness of loquat leaves.In agriculture,5-aminolevulinic acid(ALA),a potential multifunctional plant growth regulator,can improve the stress response of plants.Among the 9 low-temperature-responsive EjGSTs,the expression of EjGSTU1 and EjGSTF1 significantly differed under cold stress in response to exogenous 5-aminolevulinic acid(ALA)pretreat-ment.The remarkable increase in GST activity and GSH/GSSG ratio reflected the increase in the cold response ability of loquat plants caused by exogenous ALA,thereby alleviating H2O2 accumulation and membrane lipid preoxidation.Overall,this study provides an initial exploration of the cold tolerance function of GSTs in loquat,offering a theoretical foundation for the development of cold-resistant loquat cultivars and new antifreeze agents.
文摘The Alaskapox virus(AKPV)is a new Orthopoxvirus first identified in 2015 on a woman near Fairbanks,Alaska^([1]).This double-stranded DNA virus,part of the Poxviridae family,shares its genus with notable viruses like smallpox,monkeypox,and cowpox.Despite these genetic ties,AKPV is distinct and potentially represents a new species within the Orthopoxvirus genus,as suggested by its sequenced genome^([2]).
文摘为了更好地了解数字时代美国年轻人对图书馆和媒体的态度,揭示其行为特征,美国图书馆协会(American Library Association, ALA)以2022年的一项全国性调查为基础,于2023年11月1日发布了一份研究报告《Z世代和千禧一代:他们如何使用公共图书馆和经由媒体使用定义身份》(Gen Z and Millennials:How They Use Public Libraries and Identify Through Media Use)。这里的Z世代(Gen Z)是指2022年调查时13—25岁的人,千禧一代(Millennials)是指2022年调查时26—40岁的人。
