<strong>Background</strong>: Many literature reviews report vitamin E supplementation as a benefit chemopreventive and adjuvant therapy in breast and prostate cancers. We investigated in the present work, ...<strong>Background</strong>: Many literature reviews report vitamin E supplementation as a benefit chemopreventive and adjuvant therapy in breast and prostate cancers. We investigated in the present work, alpha-tocopherol (<span style="white-space:nowrap;">α</span>-T) expression (the current active form of vitamin E) from tissues of Congolese patients neither smokers nor alcohol drinkers and without intake antioxidant vitamins supplement. <strong>Methods</strong>: <span style="white-space:nowrap;">α</span>-T was analysed in one normal placenta of nine weeks of gestation and in nineteen cancerous tissues, including seven breast cancers, six prostate cancers, and six gestational choriocarcinomas. The study was performed by immunohistochemistry method after diagnosis confirmation by histological analysis. <strong>Results</strong>: <span style="white-space:nowrap;">α</span>-T staining in membrane cells and collagen fibers presented a moderate expression in healthy sections of tissues (positive control), but the labelling was strong in breast, prostate adenocarcinomas, and in choriocarcinomas. <strong>Conclusion</strong>: Tumors immunohistochemistry of <span style="white-space:nowrap;">α</span>-tocopherol in breast, prostate cancers and in choriocarcinoma show elevated immunostaining suggesting a probable oncogenic effect of the micronutrient.展开更多
Objective:To observe the effects of vitamin E on post-vitrification preimplantation development,gross morphology as well as mitochondrial distribution and ultrastructure.Methods:Twenty-four female C57BL/6NTac mice,age...Objective:To observe the effects of vitamin E on post-vitrification preimplantation development,gross morphology as well as mitochondrial distribution and ultrastructure.Methods:Twenty-four female C57BL/6NTac mice,aged 12-16 weeks,were randomly divided into four groups.Group A did not receive any treatment and served as the control group.Group B was treated with corn oil stripped of tocopherols and served as the vehicle group.Group C was treated with 60 mg/kg body weight of tocotrienol-rich-fraction with corn oil stripped of tocopherols.Group D was treated with 60 mg/kg body weight of alpha-tocopherol with corn oil stripped of tocopherols.All treatments were administered orally for 7 consecutive days.After superovulation and mating with fertile males,2-cell stage embryos were harvested for vitrification.Post vitrification development in vitro,gross morphology and ultrastructure were compared between groups.Results:The number of 2 and 8-cell embryo,and blastocysts in the treatment and control groups were not significantly different(P>0.05).Following vitrification,all 2-cell embryos had equal-sized blastomeres and intact zona pellucida.Mitochondrial aggregation toward the perinuclear region was seen in all of the treatment groups.Both groups C and D had vacuolated mitochondria,which was reflected in the trend of preimplantation development reduction.Conclusions:Vitamin E supplementation of 60 mg/kg body weight does not improve the viability of healthy embryos according to this study.As a result,the most effective dose of vitamin E supplementation may be determined by the initial quality of the embryos.展开更多
The supplementation of vitamin E on broiler chicken diets is essential to the prevention of lipid oxidation reactions in the meat and improvement of meat quality.The objective of this study was to assess the effect of...The supplementation of vitamin E on broiler chicken diets is essential to the prevention of lipid oxidation reactions in the meat and improvement of meat quality.The objective of this study was to assess the effect of different doses of dietary vitamin E on breast meat quality of broiler chickens in the finishing period.Five doses of vitamin E were used(30,90,150,210,and 270 mg/kg feed)in broilers'diets from 42 to 54 d of age.A completely randomized design was conducted,followed by a split-plot,where the vitamin E dose was considered as the whole plot,and broilers'age at slaughter was the subplot.Breast meat quality was assessed at 4 different ages(45,48,51,and 54 d old),using 50 birds per age,totaling 200 birds.Meat quality characteristics evaluated were:pH at 24 h post mortem,color(brightness,redness,and yellowness),water holding capacity,cooking loss,shear force,and lipid peroxidation.There was no interaction between age and dose of vitamin E for meat quality characteristics(P>0.05).The age at slaughter had a quadratic effect(P<0.05)on pH,brightness,redness,and water holding capacity.Although pH values were higher in the breast meat of older birds(51 and 52 d old),breast meat of younger birds(48 d)had a more reddish aspect.Shear force value was higher in breast meat of birds slaughtered at later ages(P<0.01),as a linear age-effect was observed.Brightness increased linearly(P<0.05)with higher vitamin doses,whereas treatments did not alter yellowness,cooking loss,and lipid peroxidation.In this study,increasing vitamin E doses in the finishing period increased the brightness of broiler breast meat,whereas slaughtering at later ages resulted in greater meat pH and shear force value.展开更多
Targetingmitochondria via nano platform emerged as an attractive anti-tumor pathway due to the central regulation role in cellar apoptosis and drug resistance.Here,a mitochondria-targeting nanoparticle(TOS-PDA-PEG-TPP...Targetingmitochondria via nano platform emerged as an attractive anti-tumor pathway due to the central regulation role in cellar apoptosis and drug resistance.Here,a mitochondria-targeting nanoparticle(TOS-PDA-PEG-TPP)was designed to precisely deliver polydopamine(PDA)as the photothermal agent and alphatocopherol succinate(α-TOS)as the chemotherapeutic drug to the mitochondria of the tumor cells,which inhibits the tumor growth through chemo-and photothermal-synergistic therapies.TOSPDA-PEG-TPP was constructed by coating PDA on the surface of TOS NPs self-assembled byα-TOS,followed by grafting PEGand triphenylphosphonium(TPP)on their surface to prolong the blood circulation time and target delivery of TOS and PDA to the mitochondria of tumor cells.In vitro studies showed that TOS-PDA-PEGTPP could be efficiently internalized by tumor cells and accumulated atmitochondria,resulting in cellular apoptosis and synergistic inhibition of tumor cell proliferation.In vivo studies demonstrated that TOS-PDA-PEG-TPP could be efficiently localized at tumor sites and significantly restrain the tumor growth under NIR irradiation without apparent toxicity or deleterious effects.Conclusively,the combination strategy adopted for functional nanodrugs construction aimed at target-delivering therapeutic agents with different action mechanisms to the same intracellular organelles can be extended to other nanodrugs-dependent therapeutic systems.展开更多
文摘<strong>Background</strong>: Many literature reviews report vitamin E supplementation as a benefit chemopreventive and adjuvant therapy in breast and prostate cancers. We investigated in the present work, alpha-tocopherol (<span style="white-space:nowrap;">α</span>-T) expression (the current active form of vitamin E) from tissues of Congolese patients neither smokers nor alcohol drinkers and without intake antioxidant vitamins supplement. <strong>Methods</strong>: <span style="white-space:nowrap;">α</span>-T was analysed in one normal placenta of nine weeks of gestation and in nineteen cancerous tissues, including seven breast cancers, six prostate cancers, and six gestational choriocarcinomas. The study was performed by immunohistochemistry method after diagnosis confirmation by histological analysis. <strong>Results</strong>: <span style="white-space:nowrap;">α</span>-T staining in membrane cells and collagen fibers presented a moderate expression in healthy sections of tissues (positive control), but the labelling was strong in breast, prostate adenocarcinomas, and in choriocarcinomas. <strong>Conclusion</strong>: Tumors immunohistochemistry of <span style="white-space:nowrap;">α</span>-tocopherol in breast, prostate cancers and in choriocarcinoma show elevated immunostaining suggesting a probable oncogenic effect of the micronutrient.
基金This research was supported financially by Universiti Teknologi MARA Grants[600-IRMI/REI/5/3(022/2019)]and[600-IRMI/MYRA 5/3/MITRA(008/2017)-2].
文摘Objective:To observe the effects of vitamin E on post-vitrification preimplantation development,gross morphology as well as mitochondrial distribution and ultrastructure.Methods:Twenty-four female C57BL/6NTac mice,aged 12-16 weeks,were randomly divided into four groups.Group A did not receive any treatment and served as the control group.Group B was treated with corn oil stripped of tocopherols and served as the vehicle group.Group C was treated with 60 mg/kg body weight of tocotrienol-rich-fraction with corn oil stripped of tocopherols.Group D was treated with 60 mg/kg body weight of alpha-tocopherol with corn oil stripped of tocopherols.All treatments were administered orally for 7 consecutive days.After superovulation and mating with fertile males,2-cell stage embryos were harvested for vitrification.Post vitrification development in vitro,gross morphology and ultrastructure were compared between groups.Results:The number of 2 and 8-cell embryo,and blastocysts in the treatment and control groups were not significantly different(P>0.05).Following vitrification,all 2-cell embryos had equal-sized blastomeres and intact zona pellucida.Mitochondrial aggregation toward the perinuclear region was seen in all of the treatment groups.Both groups C and D had vacuolated mitochondria,which was reflected in the trend of preimplantation development reduction.Conclusions:Vitamin E supplementation of 60 mg/kg body weight does not improve the viability of healthy embryos according to this study.As a result,the most effective dose of vitamin E supplementation may be determined by the initial quality of the embryos.
文摘The supplementation of vitamin E on broiler chicken diets is essential to the prevention of lipid oxidation reactions in the meat and improvement of meat quality.The objective of this study was to assess the effect of different doses of dietary vitamin E on breast meat quality of broiler chickens in the finishing period.Five doses of vitamin E were used(30,90,150,210,and 270 mg/kg feed)in broilers'diets from 42 to 54 d of age.A completely randomized design was conducted,followed by a split-plot,where the vitamin E dose was considered as the whole plot,and broilers'age at slaughter was the subplot.Breast meat quality was assessed at 4 different ages(45,48,51,and 54 d old),using 50 birds per age,totaling 200 birds.Meat quality characteristics evaluated were:pH at 24 h post mortem,color(brightness,redness,and yellowness),water holding capacity,cooking loss,shear force,and lipid peroxidation.There was no interaction between age and dose of vitamin E for meat quality characteristics(P>0.05).The age at slaughter had a quadratic effect(P<0.05)on pH,brightness,redness,and water holding capacity.Although pH values were higher in the breast meat of older birds(51 and 52 d old),breast meat of younger birds(48 d)had a more reddish aspect.Shear force value was higher in breast meat of birds slaughtered at later ages(P<0.01),as a linear age-effect was observed.Brightness increased linearly(P<0.05)with higher vitamin doses,whereas treatments did not alter yellowness,cooking loss,and lipid peroxidation.In this study,increasing vitamin E doses in the finishing period increased the brightness of broiler breast meat,whereas slaughtering at later ages resulted in greater meat pH and shear force value.
基金supported by the Medico-Engineering Cooperation Funds from the University of Electronic Science and Technology of China(ZYGX2021YGCX018)Sichuan Provincial Science Fund for applied basic research of China(2020YJ0108)Sichuan Provincial Science Fund for applied basic research of China(2020YFS0424).
文摘Targetingmitochondria via nano platform emerged as an attractive anti-tumor pathway due to the central regulation role in cellar apoptosis and drug resistance.Here,a mitochondria-targeting nanoparticle(TOS-PDA-PEG-TPP)was designed to precisely deliver polydopamine(PDA)as the photothermal agent and alphatocopherol succinate(α-TOS)as the chemotherapeutic drug to the mitochondria of the tumor cells,which inhibits the tumor growth through chemo-and photothermal-synergistic therapies.TOSPDA-PEG-TPP was constructed by coating PDA on the surface of TOS NPs self-assembled byα-TOS,followed by grafting PEGand triphenylphosphonium(TPP)on their surface to prolong the blood circulation time and target delivery of TOS and PDA to the mitochondria of tumor cells.In vitro studies showed that TOS-PDA-PEGTPP could be efficiently internalized by tumor cells and accumulated atmitochondria,resulting in cellular apoptosis and synergistic inhibition of tumor cell proliferation.In vivo studies demonstrated that TOS-PDA-PEG-TPP could be efficiently localized at tumor sites and significantly restrain the tumor growth under NIR irradiation without apparent toxicity or deleterious effects.Conclusively,the combination strategy adopted for functional nanodrugs construction aimed at target-delivering therapeutic agents with different action mechanisms to the same intracellular organelles can be extended to other nanodrugs-dependent therapeutic systems.
