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北方黑土地花生根际丛枝菌根菌(AMF)分离鉴定及应用 被引量:1
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作者 王雪霏 曹哲 +7 位作者 杨钥 周馨瞳 王子郡 邵文杰 周子恺 徐佳璐 孟令波 郑苗苗 《高师理科学刊》 2022年第8期71-76,共6页
采集黑龙江省种植花生区域的黑土土样,通过湿筛法从花生根际土样中分离纯化出AMF菌孢子,根据形态学特征和分子生物学进行鉴定,获得一株AMF菌,为株幼套球囊霉(Glomus etunicatum),通过Single spore isolating方法完成AMF菌培养繁殖,孢子... 采集黑龙江省种植花生区域的黑土土样,通过湿筛法从花生根际土样中分离纯化出AMF菌孢子,根据形态学特征和分子生物学进行鉴定,获得一株AMF菌,为株幼套球囊霉(Glomus etunicatum),通过Single spore isolating方法完成AMF菌培养繁殖,孢子量350个/g,将获得的纯净孢子接种到以花生为宿主植物的盆钵中,进行扩大繁殖.AMF菌能够促进花生根系伸长生长,提高根系对N,P,K无机盐离子的吸收,而且还具有抗倒伏作用,提高了花生产量,同时提高花生的油脂含量,能使花生中油脂含量提高2.3%~2.7%. 展开更多
关键词 amf菌 东北四粒红花生 油脂
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Review of Research Methods on Biodiversity of Arbuscular Mycorrhizal Fungi 被引量:1
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作者 李凌飞 付晓萍 董文明 《Agricultural Science & Technology》 CAS 2012年第3期600-604,共5页
Arbuscular mycorrhizal fungi (AMF) are essential functional microbiology in natural ecosystems. It is very important to research community composition and di- versity of AMF for achieving sustainable development of ... Arbuscular mycorrhizal fungi (AMF) are essential functional microbiology in natural ecosystems. It is very important to research community composition and di- versity of AMF for achieving sustainable development of ecosystems. The paper described several methods of researching the diversity of AMF, especially for molecular techniques, reviewed the application status of these methods in AMF research, and pointed out that the effective combination of morphological and molecular methods could better reveal the biodiversity and ecological functions of AMF in natural ecosystems. 展开更多
关键词 Arbuscular Mycorrhizal Fungi amf BIODIVERSITY Morphological method Phospholipid fatty acid (PLFA) method Molecular biotechnology
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