Chronic myeloid leukemia（CML） is characterized by the accumulation of active BCR-ABL protein. Imatinib is the first-line treatment of CML; however, many patients are resistant to this drug. In this study, we aimed t...Chronic myeloid leukemia（CML） is characterized by the accumulation of active BCR-ABL protein. Imatinib is the first-line treatment of CML; however, many patients are resistant to this drug. In this study, we aimed to compare the differences in expression patterns and functions of time-series genes in imatinib-resistant CML cells under different drug treatments. GSE24946 was downloaded from the GEO database, which included 17 samples of K562-r cells with（n=12） or without drug administration（n=5）. Three drug treatment groups were considered for this study： arsenic trioxide（ATO）, AMN107, and ATO＋AMN107. Each group had one sample at each time point（3, 12, 24, and 48 h）. Time-series genes with a ratio of standard deviation/average（coefficient of variation） 〉0.15 were screened, and their expression patterns were revealed based on Short Time-series Expression Miner（STEM）. Then, the functional enrichment analysis of time-series genes in each group was performed using DAVID, and the genes enriched in the top ten functional categories were extracted to detect their expression patterns. Different time-series genes were identified in the three groups, and most of them were enriched in the ribosome and oxidative phosphorylation pathways. Time-series genes in the three treatment groups had different expression patterns and functions. Time-series genes in the ATO group（e.g. CCNA2 and DAB2） were significantly associated with cell adhesion, those in the AMN107 group were related to cellular carbohydrate metabolic process, while those in the ATO＋AMN107 group（e.g. AP2M1） were significantly related to cell proliferation and antigen processing. In imatinib-resistant CML cells, ATO could influence genes related to cell adhesion, AMN107 might affect genes involved in cellular carbohydrate metabolism, and the combination therapy might regulate genes involved in cell proliferation.展开更多
This article shows the quality of services in a wireless swarm of drones that form an ad hoc network between them Fly Ad Hoc Networks(FANET).Each drone has the ability to send and receive information(like a router);an...This article shows the quality of services in a wireless swarm of drones that form an ad hoc network between them Fly Ad Hoc Networks(FANET).Each drone has the ability to send and receive information(like a router);and can behave as a hierarchical node whit the intregration of three protocols:Multiprotocol Label Switch(MPLS),Fast Hierarchical AD Hoc Mobile(FHAM)and Internet Protocol version 6(IPv6),in conclusion MPLS+FHAM+IPv6.The metrics analyzed in the FANET are:delay,jitter,throughput,lost and sent packets/received.Testing process was carried out with swarms composed of 10,20,30 and 40 units;In this work,the stage with 40 droneswas analyzed showing registration processes,and sentmessages sequences between different drones that were part of the same swarm.A special analysis about the traffic between drones(end-to-end)was carried out,as well as the possible security flaws in each drone and the current status and future trends in real services.Regarding future trends,in a real environment,we took as a starting point,metrics results obtained in the simulation(positive according to the obtained results).These results gave us a clear vision of how the network will behave in a real environment with the aim to carry out the experiment on a physical level in the near future.This work also shows the experience quality from the service quality metrics obtained through a mathematical model.This quality of experience model will allow us to use it objectively in the agricultural sector,which is a great interest area and is where we are working with drones.Finally in this article we show our advances for a business model applied to the aforementioned agricultural sector,as well as the data analysis and services available to the end customer.These services available to the end customer have been classified into a basic,medium,advanced and plus level.展开更多
基金supported by Natural Science Foundation of Heilongjiang Province of China(No.D201252)
文摘Chronic myeloid leukemia（CML） is characterized by the accumulation of active BCR-ABL protein. Imatinib is the first-line treatment of CML; however, many patients are resistant to this drug. In this study, we aimed to compare the differences in expression patterns and functions of time-series genes in imatinib-resistant CML cells under different drug treatments. GSE24946 was downloaded from the GEO database, which included 17 samples of K562-r cells with（n=12） or without drug administration（n=5）. Three drug treatment groups were considered for this study： arsenic trioxide（ATO）, AMN107, and ATO＋AMN107. Each group had one sample at each time point（3, 12, 24, and 48 h）. Time-series genes with a ratio of standard deviation/average（coefficient of variation） 〉0.15 were screened, and their expression patterns were revealed based on Short Time-series Expression Miner（STEM）. Then, the functional enrichment analysis of time-series genes in each group was performed using DAVID, and the genes enriched in the top ten functional categories were extracted to detect their expression patterns. Different time-series genes were identified in the three groups, and most of them were enriched in the ribosome and oxidative phosphorylation pathways. Time-series genes in the three treatment groups had different expression patterns and functions. Time-series genes in the ATO group（e.g. CCNA2 and DAB2） were significantly associated with cell adhesion, those in the AMN107 group were related to cellular carbohydrate metabolic process, while those in the ATO＋AMN107 group（e.g. AP2M1） were significantly related to cell proliferation and antigen processing. In imatinib-resistant CML cells, ATO could influence genes related to cell adhesion, AMN107 might affect genes involved in cellular carbohydrate metabolism, and the combination therapy might regulate genes involved in cell proliferation.
基金This research has been funded by Dirección General de Investigaciones of Universidad Santiago de Cali under Call No.01-2021.
文摘This article shows the quality of services in a wireless swarm of drones that form an ad hoc network between them Fly Ad Hoc Networks(FANET).Each drone has the ability to send and receive information(like a router);and can behave as a hierarchical node whit the intregration of three protocols:Multiprotocol Label Switch(MPLS),Fast Hierarchical AD Hoc Mobile(FHAM)and Internet Protocol version 6(IPv6),in conclusion MPLS+FHAM+IPv6.The metrics analyzed in the FANET are:delay,jitter,throughput,lost and sent packets/received.Testing process was carried out with swarms composed of 10,20,30 and 40 units;In this work,the stage with 40 droneswas analyzed showing registration processes,and sentmessages sequences between different drones that were part of the same swarm.A special analysis about the traffic between drones(end-to-end)was carried out,as well as the possible security flaws in each drone and the current status and future trends in real services.Regarding future trends,in a real environment,we took as a starting point,metrics results obtained in the simulation(positive according to the obtained results).These results gave us a clear vision of how the network will behave in a real environment with the aim to carry out the experiment on a physical level in the near future.This work also shows the experience quality from the service quality metrics obtained through a mathematical model.This quality of experience model will allow us to use it objectively in the agricultural sector,which is a great interest area and is where we are working with drones.Finally in this article we show our advances for a business model applied to the aforementioned agricultural sector,as well as the data analysis and services available to the end customer.These services available to the end customer have been classified into a basic,medium,advanced and plus level.
