Human amnion tissue injected with human umbilical cord mesenchymal stem cells repairs damaged sciatic nerves in rats

Human umbilical cord mesenchymal stem cells,incorporated into an amnion carrier tubes,were assessed for nerve regeneration potential in a rat nerve defect model.Damaged nerves were exposed to human amnion carriers containing either human umbilical cord mesenchymal stem cell （cell transplantation group）or saline（control group）.At 8,12,16 and 20 weeks after cell implantation,the sciatic functional index was higher in the cell transplantation group compared with the control group.Furthermore,electrophysiological examination showed that threshold stimulus and maximum stimulus intensity gradually decreased while compound action potential amplitude gradually increased.Hematoxylin-eosin staining showed that regenerating nerve fibers were arranged in nerve tracts in the cell transplantation group and connective tissue between nerve tracts and amnion tissue reduced over time.Gastrocnemius muscle cell diameter,wet weight and restoration ratio were increased.These data indicate that transplanted human umbilical cord mesenchymal stem cells,using the amnion tube connection method,promote restoration of damaged sciatic nerves in rats.

Amnion epithelial cells——a novel therapy for ischemic stroke?

Stroke is a leading cause of death and disability and new therapies are desperately needed. Given the complex nature of ischemic brain injury, it has been postulated that cell-based therapies may be useful. However, cell resources, invasive extraction procedures, immunological rejection, tumorigenesis and ethical challenges make it unlikely that many stem cell types could serve as a practical source for therapy. By contrast, these issues do not pertain to human amnion epithelial cells（h AECs）, which are placenta-derived stem cells. We recently assessed the effects of systemically delivered hAECs on stroke outcome using four animal models of stroke. We demonstrated that when injected intravenously after ischemia onset, hAECs migrate preferentially to the spleen and injured brain to limit apoptosis and inflammation, and attenuate early brain infiltration of immune cells, progression of infarction and systemic immunosuppression and to ultimately ameliorate functional deficits. When administration of hAECs is delayed by 1-3 days poststroke, long-term functional recovery can still be enhanced in young and aged mice of either sex. Moreover, our proof-of-principle findings suggest that h AECs are effective at limiting post-stroke infarct development in non-human primates. Overall, the results suggest that hAECs could be a viable clinical stroke therapy.

Neuronal-like differentiation of single versus multiple treatments with human amnion-derived mesenchymal stem cells induced by basic fibroblast growth factor

BACKGROUND： Cultures from multiple portions of umbilical cord blood mesenchymal stem cells have been shown to undergo more rapid proliferation and attachment than single portions. OBJECTIVE： To observe growth of basic fibroblast growth factor （bFGF）-induced cultures of human amnion-derived mesenchymal stem cells （AMSCs） and differentiation into neuronal-like cells. DESIGN, TIME AND SETTING： Comparative observation. The study was performed at the Laboratory of Microbiology and Immunology, Basic Medical School of Zhengzhou University from January to May 2008. METHODS： Amnia from full-term, uterine-incision delivery were donated by 12 healthy women. AMSCs were obtained by cell separation and culture techniques, and were passaged and induced by bFGF. From the third passage, a total of 1 mLAMSCs, at a density of 1.0 × 10^4/mL, was separately harvested from six samples, which served as group A. A total of 1 mL AMSCs, at a density of 1.0 × 10^4/mL, was harvested separately from the remaining six samples, which served a group B. A total of 0.5 mL from the six samples of group A and 0.5 mL from the six samples of grot, B were combined to form group C. MAIN OUTCOME MEASURES： Differences in cell quantity among the three groups were compare by cell quantification and 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） analysis. Expression of a glial cell marker, neuron-specific enolase, and nestin was detected in the three groups by immunocytochemistry. RESULTS： Cell quantification and MTT analysis of live cells, as well as AMSC absorbance, were significantly greater in group C compared with groups A and B at 18 days of culture （P 〈 0.05）, anc no significant difference was observed between groups A and B. Glial fibrillary acidic protein, neuron-specific enolase, and nestin were expressed in all groups following bFGF induction. CONCLUSION： Mixed AMSC cultures promoted proliferation, and bFGF-induced AMSCs differentiated into neuronal-like cells.

Interaction between porcine granulosa and thecal cells in steroidogenesis in an amnion dual chamber culture system

Objective: To study the interaction between granulosa and thecal cel1s in steroidogenesis in an am-nion dual chamber in comparison with the cellulose dual chamber.Method: A dual chamber culture system was designed and prepared with amnion membrane from human term placenta. The isolated porcine granulosa and thecal cells were grown on both sides of the amnion membrane, with granulosa cells in the inner chamber and thecal cells in the outer chamber. The concentrations of estradiol (E,), progesterone (P) and testosterone (T) in the culture media were mea-sured by radioimmunoassay.Results: The growth of both cells and their steroidogenic function were more active in amnion dual chamber system than in cellulose dual chamber system: (1) more T produced by thecal cells in the outer chamber, passing into inner chamber through the amnion membrane. T was used by granulosa cells as the substrate of aromatization, so that granulosa cells produced more E2 (up to 2 435 pmol/L); (2) the production of P (52. 5 μmol/L) and T (10. 2μmol/L) by granulosa cells cultured in the amnion mem-brane dual chamber system were also higher.Conclusions:The dual chamber system made of amnion mpmbrane showed better effect in studying steroidogenesis than with cellulose dual chamber system, and can be used as a model for studying paracrine regulatory interaction between granulosa and thecal cells in vitro.

Effect of amnion membrane transplantation on corneal neovascularization in 10 patients with alkali burn

By observing clinical cases, we studied the curative effect of amnion membrane transplantation on decreasing corneal neovascularization (CNV). It was a non-randomized retrospective case-control study. Among 17 cases (21 eyes) of third-degree alkali burns from 2007 to 2010, 10 cases (12 eyes) were performed with amnion membrane transplantation operation, and others were not. Amnion membrane transplantation was performed at the 3rd day after burn in the treatment group. Areas of CNV in double groups were measured at the 14th day and 60th day after burn. Area of CNV in the treatment group was (66.207±7.251)mm2 at the 14th day after burn, and was 18.27% lower than that in the control group. Area of CNV in the treatment group was (120.046±13.812)mm2 at the 60th day after burn, and was 11.35% lower than that in the control group. There was both statistical significance (P<0.05). Amnion membrane transplantation operation can inhibit the growth of corneal neovascularization induced by alkali burn.

Characterization of Side Cell Populations Obtained from Human Amnion Mesenchymal Cells

Human amnion mesenchymal cells (AMCs) contain multipotent cells. To enrich such multipotent stem cells, we applied to AMCs the new method for the isolation of side population (SP) cells used for the enrichment of multipotent stem cells from many tissues. We succeeded in obtaining SP cells from AMCs (AMC-SP cells). AMC-SP cells were found in 0.2% of AMCs, irrespective of the length of pregnant period, ranging from 37 to 40 weeks. Cell cycle analyses suggested that AMC-SP cells belonged to a cell population that proliferated very slowly and/or was in a quiescent state in the amniotic membrane. Upon culturing, they proliferated with 40 to 80 cell doublings. However, they did not form colonies in a soft agarose culture, whereas HepG2 cells, representative human hepatoma cells formed many large colonies. These results suggest that AMC-SP cells that have considerable value for the use of regenerative medicine can be managed safely in vitro.

Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing

Complete skin reconstruction is a hierarchical,physiological assembly process involving healing of the epidermis,dermis,vasculature,nerves,and cutaneous appendages.To date,few works have reported complete skin regeneration,particularly lacking vascular structures and hair follicles after full skin defects.In this study,a hydrogel derived from the skin secretion of Andrias davidianus(SSAD)that features adhesiveness was used as a bioactive scaffold to load micronized amnion(MA).The SSAD hydrogel was found to promote the migration and proliferation of amnion stem cells and human keratinocytes,as well as inhibit their apoptosis in vitro.In a rat full-skin defect model,the regeneration of skin appendages was observed at the wound area,achieving scarless healing.Transcriptome analyses further validated that SSAD could positively regulate cell migration,proliferation,and differentiation.These functions might be attributed to the abundant growth factors present in the SSAD.Synergized by the delivery of MA,SSAD loaded with the MA could achieve a significantly better skin regeneration effect than SSAD or MA used alone,providing a simple yet highly effective means to obtain complete,scarless skin regeneration,suggesting favorable potential for clinical translation.

Has_circ_0008717在羊膜间充质干细胞上清促血管生成中的作用研究

目的:探究has_circ_0008717在人羊膜间充质干细胞上清(hAMSC-CM)促进人脐静脉内皮细胞(HUVECs)血管生成过程中的作用。方法:通过划痕实验、Transwell实验及成管实验检测hAMSC-CM对HUVECs迁移及成管能力的影响,实时荧光定量RT-PCR筛选上调的circRNA(has_circ_0008717);小干扰RNA(siRNA)下调HUVECs内has_circ_0008717水平后,实时荧光定量RT-PCR检测血管内皮生长因子A(VEGFA)表达水平变化,划痕实验、Transwell实验及成管实验观察其对hAMSC-CM促进HUVECs迁移及成管的影响。结果:hAMSC-CM可明显促进HUVECs迁移及成管能力(P<0.05),HUVECs中has_circ_0008717升高最为明显(P<0.05),而敲低has_circ_0008717可明显抑制hAMSC-CM促进HUVECs迁移、成管和VEGFA的表达(P<0.05)。结论:hAMSC-CM可通过has_circ_0008717增强HUVECs的成管能力。

The study on the safety and efficacy of amnion graft for preventing the recurrence of moderate to severe intrauterine adhesions

Transcervical resection of adhesion(TCRA)is the standard treatment for the intrauterine adhesions,but the recurrence of adhesions is a tough problem for the gynecologist.In addition,the therapeutic strategy after TCRA about prevention of recurrence remains controversial especially for the patients with moderate to severe intrauterine adhesions(IUAs).Hence,we designed this study to explore the safety and efficacy of fresh amnion grafts for preventing the recurrence after TCRA for patients with moderate to severe IUAs.One hundred patients with moderate to severe IUAs who presented with a history of hypomenorrhea,amenorrhea and infertility were included in the study from January 2015 to December 2017.Patients were divided into amnion group(52 patients)and chitosan group(48 patients).Fresh amnion grafts or intrauterine injections of chitosan were administered after TCRA.Transvaginal ultrasonography(TVUS)and hysteroscopy were performed at the first and third month after the operation.The surgical procedures for all patients were completed successfully without relevant complications.In amnion group,8 patients exhibited relapse in the first month and 2 patients in three months after surgery;in chitosan group,23 women exhibited relapse in the first month and 18 patients in three months after surgery.Statistical analysis revealed that the recurrence rate of adhesion in amnion group was significantly lower than those of chitosan group in the first and three months after surgery(P1=0.000,P2=0.000).After TCRA,fresh amnion graft plays a significant role in preventing further adhesions than injections of chitosan.

A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal stromal keratocytes for stromal cell therapy

Background:Human corneal stromal keratocytes propagated in culture media supplemented with human amnion extract(AME)can correct early corneal haze in an animal model.Clinical application of cultivated keratocytes is limited by infectious disease screening before amnion products can be used in humans.It remains unclear if AME from cryopreserved versus fresh human amnion can support human keratocyte propagation,and which components of the extract promote keratocyte growth.Methods:Three placentas were collected for the preparation of fresh and cryopreserved amnion tissues followed by homogenization and protein extraction.AME protein profiles were studied using isobaric tagging for relative and absolute quantitation(iTRAQ)proteomics.Enriched gene ontology(GO)terms and functional classes were identified.Primary human keratocytes from 4 donor corneas were cultured in media supplemented with fresh AME(F-AME)or cryopreserved AME(C-AME).Cell viability,proliferation and keratocyte marker expression were examined by confocal immunofluorescence and flow cytometry.Results:AME proteomics revealed 1385 proteins with similar expression levels(between 0.5-and 2-fold)between Fand C-AME,while 286 proteins were reduced(less than 0.5-fold)in C-AME.Enriched GO term and biological pathway analysis showed that those proteins with comparable expression between F-AME and C-AME were involved in cell metabolism,epithelial-mesenchymal transition,focal adhesion,cell-extracellular matrix interaction,cell stress regulation and complement cascades.Human corneal stromal keratocytes cultured with F-AME or C-AME showed similar morphology and viability,while cell proliferation was mildly suppressed with C-AME(P>0.05).Expression of aldehyde dehydrogenase 3A1(ALDH3A1)and CD34 was similar in both cultures.Conclusion:AME from cryopreserved amnion had limited influence on keratocyte culture.It is feasible to use protein extract from cryopreserved amnion to propagate human keratocytes for potential translational applications.

生物羊膜在手外科断指再植术中的应用效果分析

目的探讨生物羊膜在手外科断指再植术中的应用效果。方法60例接受手部断指再植术患者为研究对象,按照随机数字表法分为对照组和实验组,每组30例。对照组采用断指再植术治疗,实验组在对照组基础上联合生物羊膜治疗。比较两组术后2、4个月手指总主动活动度(TAM)、断指成活率及肌腱、神经血管粘连情况。结果实验组术后2、4个月手指TAM分别为(185.2±10.6)、(221.4±12.2)°,大于对照组的(174.5±9.7)°、(205.5±15.8)°,差异有统计学意义(P<0.05)。治疗10 d后,两组断指成活率比较差异无统计学意义(P>0.05);实验组肌腱粘连率和神经粘连率分别为3.33%、6.67%,低于对照组的20.00%、26.67%,差异有统计学意义(P<0.05)。结论断指再植术中应用生物羊膜治疗不影响断指成活率,且能有效防止肌腱和神经血管粘连,值得临床推广应用。

人参皂苷Rg1联合人羊膜间充质干细胞移植治疗卵巢功能不全的效用及机制

目的:评估人参皂苷Rg1(Ginsenoside Rg1)联合人羊膜间充质干细胞(hAD-MSCs)移植治疗卵巢功能不全(POI)的疗效优势。方法:共使用40只大鼠,随机分为对照(Control)组、模型(Model)组、单纯hAD-MSCs治疗(hAD-MSCs)组和hAD-MSCs联合Rg1治疗(hAD-MSCs+Rg1)组,每组10只,其中5只用于采集卵巢和血液等组织样本,另5只进行卵母细胞诱导和卵母细胞线粒体功能测定相关实验。模型组构建放疗辐射诱导的POI大鼠模型。结果:与Control组相比,Model组大鼠卵巢存在较大程度的卵泡耗竭和器质性病变,初级卵泡、次级卵泡、窦卵泡和排卵前卵泡均减少,卵巢指数下降,血清FSH升高,E2降低,卵巢组织TUNEL阳性细胞数量增加,ROS水平上升,卵母细胞线粒体膜电势降低,ATP生成量减少(P<0.05)。与单纯hAD-MSCs组相比,hAD-MSCs+Rg1联合组POI大鼠卵巢组织卵泡耗竭和器质性病变明显缓解,初级卵泡、次级卵泡、窦卵泡和排卵前卵泡明显增加,卵巢指数回升显著,血清FSH降低,E2回升,卵巢组织TUNEL阳性细胞数量明显下降,ROS水平明显降低,卵母细胞线粒体膜电势回升,ATP生成量回升显著(P<0.05)。结论:Rg1通过增强hAD-MSCs移植改善卵母细胞线粒体功能的机制并缓解POI大鼠模型疾病进展。

生物衍生羊膜对大鼠跟腱粘连影响的实验研究

目的 研究生物衍生羊膜预防 SD大鼠跟腱粘连的效果 ,并为临床预防术后跟腱粘连提供实验数据。方法 以 2 8只 SD大鼠后肢跟腱为实验模型 ,切断并缝合跟腱 ,实验侧跟腱包裹生物衍生羊膜 ,对侧不包裹作为对照 ,术后 1、2、4、6、8和 12周分别取跟腱 ,大体及组织学观察跟腱的粘连、愈合情况。 结果 术后各时间点两侧跟腱吻合处炎性细胞浸润情况、跟腱愈合进程无明显差别 ,跟腱粘连的半定量评分表明实验侧和对照侧差异有统计学意义 ( P<0 .0 5 )。 结论 生物衍生羊膜能有效预防跟腱粘连 ,是一种较为理想的预防跟腱粘连的生物材料。

新鲜羊膜与脱细胞羊膜修复腱鞘缺损预防肌腱粘连

背景:羊膜独有的结构可阻止某些物质通过,能保证包裹内组织正常营养供应,而且具有抗粘连、组织相容性好、炎性反应轻、纤维包裹少及可降解等特性。目的:比较新鲜羊膜及脱细胞羊膜修复腱鞘缺损,预防肌腱粘连和促进肌腱愈合的作用。方法:取60只雄性来亨鸡,制作双足第三足趾制备肌腱及腱鞘损伤模型,随机分为3组修复,新鲜羊膜组采用新鲜人羊膜修复腱鞘缺损,脱细胞羊膜组采用人脱细胞羊膜修复腱鞘缺损,对照组不做腱鞘修复。修复后进行第三足趾组织学观察及生物力学测试。结果与结论:①组织学观察:修复后2周,3组均存在充血水肿及炎症反应,新鲜羊膜组最轻,对照组最严重,3组水肿及炎症反应随时间延长逐渐减轻。修复12周,各组假鞘较修复后4周明显成熟,新鲜羊膜组及脱细胞羊膜组假鞘表面细胞致密层状排列整齐,表面光滑;对照组假鞘表面细胞排列紊乱,结构松散,可见表面纤维组织突出假鞘表面;②生物力学测试:脱细胞羊膜组、新鲜羊膜组修复后4,8,12周的肌腱滑动距离均大于对照组(P<0.05),前2组间比较差异无显著性意义;脱细胞羊膜组、新鲜羊膜组修复后4,8周的肌腱最大拉伸断裂强度高于对照组(P<0.05),且新鲜羊膜组高于脱细胞羊膜组(P<0.05),3组修复后12周的肌腱最大拉伸断裂强度无差异;③结果表明:新鲜羊膜和脱细胞羊膜均可用于重建腱鞘缺损,预防肌腱粘连,新鲜羊膜在促进早期肌腱愈合方面优于脱细胞羊膜。

胚胎干细胞源性表皮干细胞在肾被囊微环境中分化潜能的研究

目的探讨胚胎干细胞源性表皮干细胞在肾被囊微环境中的分化情况,为研究其在不同微环境中的分化潜能和稳定性及寻找新的皮肤工程种子细胞奠定基础。方法129小鼠E14胚胎干细胞与人羊膜共培养4d,定向诱导其分化为呈β1整合素、CK15和CK19阳性的表皮样干细胞克隆,无菌手术下移植入129小鼠双侧肾被囊内,术后4周、6周和8周取材。对移植后细胞的分化情况进行形态学和CEA和CK18免疫组织化学观察。结果小鼠胚胎干细胞源性表皮干细胞在129小鼠肾被囊内4周,分化为由单层或复层上皮样细胞构成的管状和泡状结构,种植6周和8周,除上述结构外,可见角化复层扁平上皮、汗腺样、皮脂腺样及毛囊样等结构。免疫组化结果汗腺样结构呈CEA和CK18阳性。结论研究结果表明小鼠胚胎干细胞源性表皮干细胞在肾被囊微环境下,可具有分化为角化复层上皮、汗腺样、皮脂腺样及毛囊样结构的潜能。

人羊膜诱导胚胎干细胞向表皮样干细胞的定向分化

【目的】探索体外定向诱导胚胎干细胞 (ES细胞 )分化为表皮样干细胞的条件 ,为研究其分化机理及寻找新的皮肤组织工程种子细胞奠定基础。【方法】将小鼠胚胎干细胞单独 (对照组 )或与人羊膜共培养 4～ 5d ,观察其形态分化 ,分别用β1整合素免疫组化和流式细胞仪检测胚胎干细胞向表皮样干细胞的分化。【结果】与人羊膜共培养 4～ 5d后 ,ES细胞分化为表皮细胞样的单层细胞 ,细胞排列紧密 ,呈多边形 ,免疫组织化学染色后 ,多数细胞呈现 β1整合素阳性 ,对照组大量细胞死亡 ,未见 β1整合素阳性细胞 ;流式细胞仪检测结果显示 :实验组和对照组β1整合素阳性细胞分别为 81 4%和 8 4% ,两者比较差异显著 ,Z检验P <0 0 1。

体外定向诱导人羊膜间充质干细胞向骨、软骨及脂肪细胞的分化

背景:人羊膜间充质干细胞属于成体干细胞,在体外不同条件下诱导分化为骨、软骨及其他结缔组织,较其他类型间充质干细胞,因其来源广泛,分离无创伤,较低的免疫原性、生长周期短等特点,是组织工程种子细胞的重要来源。目的:探讨人羊膜间充质干细胞是否具有间充质干细胞特性及诱导成骨、软骨及脂肪细胞诱导分化的能力。方法:取足月产胎盘羊膜组织,酶消化法分离人羊膜间充质干细胞,取第3代细胞分别加入成骨、成软骨及成脂诱导液的培养基进行诱导。结果与结论:(1)茜素红染色显示,成骨诱导后细胞存在多个矿化结节,细胞中碱性磷酸酶活性明显增加;(2)甲苯胺蓝染色显示,成软骨诱导后细胞基质中分泌大量的糖胺聚糖;(3)油红O染色显示,成脂诱导后,细胞浆内可见脂滴;(4)实时荧光PCR结果显示,成骨、成软骨及成脂诱导21 d后,相关基因表达较7 d时明显增高;(5)结果人羊膜间充质干细胞具有向成骨细胞,软骨细胞及脂肪细胞的潜能,可选作为组织工程学的种子细胞。

脱细胞羊膜与医用膜修复腱鞘缺损防治肌腱粘连的比较

背景:实验证实生物膜可用于腱鞘缺损的重建,抑制肌腱外源性愈合,为肌腱滑动提供良好的基床,减轻肌腱粘连。目的:比较脱细胞羊膜与医用膜修复腱鞘缺损中防治肌腱粘连的作用。方法:于66只来亨鸡双足第Ⅲ趾制备肌腱损伤、腱鞘缺损模型,随机分为3组,每组22只,羊膜组采用脱细胞羊膜修复腱鞘缺损,医用膜组采用可吸收医用膜修复腱鞘缺损,对照组不修复腱鞘。术后2,4,8,12周取各组标本,进行大体、组织学及生物力学检测。结果与结论:(1)大体观察:术后12周,羊膜组、医用膜组腱鞘完整,肌腱愈合良好,无粘连;对照组肌腱粘连严重。(2)组织学:术后8周,羊膜组假鞘中滑膜细胞数最多,基质内粗面内质网高度扩张,分泌旺盛,医用膜组次之,对照组滑膜细胞数最少,分布杂乱,基质内扩张空泡较前两组弱。术后12周羊膜组、医用膜组成纤维细胞层状整齐排列,结构致密;对照组腱鞘组织结构疏松,成纤维细胞分布絮乱。(3)生物力学:羊膜组、医用膜组肌腱滑动距离、总屈趾角度均大于对照组(P<0.05),前两组间比较差异无显著性意义。术后12周,3组间最大拉伸断裂强度比较差异无显著性意义。(4)结果表明,脱细胞羊膜与医用膜重建腱鞘缺损均能明显屏障肌腱外源性愈合,防治肌腱粘连。

牛羊膜在烧伤创面的临床应用研究

目的 观察牛羊膜应用于烧伤创面的临床效果，并筛选最佳制备和储存方法。方法 2004年1月～2005年11月，随机选取58例浅Ⅰ度、深Ⅱ度、Ⅲ度切削痂植皮创面、供皮区及残余创面的患者，采用同体对照，将每例患者相同性质的创面等分为3分，分别贴附经0．1％洗必泰处理（A）、0．4％戊二醛处理（B）的牛羊膜和作为对照的凡士林油纱敷料（C），比较各组的临床效果，并对制备和储存牛羊膜的方法作出评价。结果 材料A较材料B质地柔软，随意性好，应用不需预处理。临床应用见：A、B组材料换药时患者疼痛轻微，C组较明显；A、B组创面愈合时间在各类创面中均低于C组，差异有统计学意义（P〈0．01），A、B组间比较，差异无统计学意义（P〉0．05）。A、B组创面感染率在深Ⅰ度和残余创面明显低于C组，差异有统计学意义（P〈0．05）；在浅Ⅰ度、Ⅲ度切削痂植皮创面和供皮区创面与C组比较，差异无统计学意义（P〉0．05），A、B组间比较，差异无统计学意义（P〉0．05）。结论 牛羊膜可以促进烧伤创面愈合，降低创面感染率，应用于常见的烧伤创面，戊二醛处理和洗必泰处理的牛羊膜疗效相似，但后者更易推广。

羊膜植片在穿透性滤过手术中抑制巩膜瓣下纤维增殖的研究

目的探讨羊膜植片在兔眼穿透性滤过手术中抗纤维增殖情况。方法将羊膜上皮面朝外折叠成双层植入穿透性滤过手术的兔眼浅层巩膜瓣下。于术后不同时间点,行眼球组织学切片及运用显微图像分析系统对滤过道内成纤维细胞进行记数、分析。结果术后2周滤过道内开始出现成纤维细胞,随时间延长成纤维细胞数目逐渐增多,但无统计学差异(P>0.05)。对照组由术后1周开始出现纤维增殖,且逐渐加重,术后2周滤过道大部分封闭,术后3周已有新生血管长入。实验组和对照组的成纤维细胞数在术后1、2、3、4周分别进行比较,有显著性差异(P<0.05)。结论羊膜植片应用于穿透性滤过手术,可明显抑制滤过道使成纤维细胞增殖,滤过道保持通畅。