[ Objective] The paper was to study the genetic effects of ANGPTIA gene in Guizhou yellow cattle. [ Method ] The polymorphisms of ANGPT/A gene in 46 individuals of Guarding yellow cattle and Sinan yellow cattle were d...[ Objective] The paper was to study the genetic effects of ANGPTIA gene in Guizhou yellow cattle. [ Method ] The polymorphisms of ANGPT/A gene in 46 individuals of Guarding yellow cattle and Sinan yellow cattle were detected using PCR-RFLP assay. [Result] A fragment of 260 bp was amplified from AN- GPTIA gene. After digestion by restriction endonuclease MluCl, two genotypes, AA and AB, were produced whereas BB genotype was not found. A mutation of 205T→C was observed through sequencing of different genotypes. AA genotype was dominant in both yellow cattle breeds, and A allele frequency was higher than B allele frequency. X2 test results showed that the two cattle breeds were at Hardy-Weinberg equilibrium state in the digestion site （ P 〉 0.05 ）. [Condusion] The effective molecular markers of Guizbou yellow cattle, such as growth trait and intramuscular fat content, are obtained, which will provide a theoretical basis for mo- lecular breeding of meat performance of Guizhou yellow cattle.展开更多
目的观察血管生成素样蛋白-4(Angiopoietin-like protein 4,ANGPTL4)基因在3T3-L1前脂肪细胞诱导分化过程中表达水平的变化,探讨ANGPTL4基因与脂肪细胞分化的关系。方法体外培养3T3-L1前脂肪细胞,在3T3-L1前脂肪细胞分化成熟过程中(0-10...目的观察血管生成素样蛋白-4(Angiopoietin-like protein 4,ANGPTL4)基因在3T3-L1前脂肪细胞诱导分化过程中表达水平的变化,探讨ANGPTL4基因与脂肪细胞分化的关系。方法体外培养3T3-L1前脂肪细胞,在3T3-L1前脂肪细胞分化成熟过程中(0-10 d),采用油红O染色,观察脂肪细胞分化及脂质积聚情况;采用Real-time PCR、逆转录PCR技术检测脂肪细胞分化标志基因(LEPTIN,ADIPONECTIN)和ANGPTL4 mRNA表达;采用Western blot检测ANGPTL4蛋白表达。结果随着脂肪细胞诱导分化逐步深入,油红O染色显示,细胞中脂滴形成逐渐增多,达到占全视野95%以上;同时瘦素(LEPTIN)和脂联素(ADIPONECTIN)基因在诱导后的第2天开始表达并逐步增多,均提示脂肪细胞分化逐步成熟。ANGPTL4 mRNA和蛋白在前脂肪细胞中低表达,分化第2-6天ANGPTL4基因表达显著上调,分化第8-10天ANGPTL4基因表达保持在较高水平并趋于稳定。结论在3T3-L1前脂肪细胞分化过程中ANGPTL4基因表达上调,ANGPTL4基因可能参与了脂肪细胞分化。展开更多
【目的】探究家兔血管生成素样蛋白4(angiopoietin-like protein 4,ANGPTL4)基因第6外显子内的多态性及其与家兔部分生长性状的关联性。【方法】采用PCR产物测序法检测家兔ANGPTL4基因外显子内的单核苷酸多态性(SNPs)位点,并应用PCRRFL...【目的】探究家兔血管生成素样蛋白4(angiopoietin-like protein 4,ANGPTL4)基因第6外显子内的多态性及其与家兔部分生长性状的关联性。【方法】采用PCR产物测序法检测家兔ANGPTL4基因外显子内的单核苷酸多态性(SNPs)位点,并应用PCRRFLP酶切分型技术对3个家兔品种(天府黑兔、伊拉兔和新西兰兔)共495个群体样本进行酶切分型,分析SNPs与3个家兔品种部分生长性状的关联性。【结果】ANGPTL4基因第6外显子中共检测发现3个遗传变异位点(c.823 G>A为非同义突变,c.867 T>C和c.975 T>C均为同义突变),且完全处于连锁状态(r2≥0.75),由此选择c.823 G>A位点作为遗传效应分析位点。关联性分析得出3个家兔群体内AG基因型和GG基因型与3个品种部分生长性状具有较高的遗传效应。【结论】ANPGTL4基因可以是与家兔生长发育相关的候选基因,以此为培育肉兔新品种提供辅助分子遗传选择依据。展开更多
基金Supported by Science and Technology Fund of Guizhou Province[QKH J LNK(2013)No.6]Construction of Beef Cattle Industry Research System of Guizhou Province(GZCYTX-0301)
文摘[ Objective] The paper was to study the genetic effects of ANGPTIA gene in Guizhou yellow cattle. [ Method ] The polymorphisms of ANGPT/A gene in 46 individuals of Guarding yellow cattle and Sinan yellow cattle were detected using PCR-RFLP assay. [Result] A fragment of 260 bp was amplified from AN- GPTIA gene. After digestion by restriction endonuclease MluCl, two genotypes, AA and AB, were produced whereas BB genotype was not found. A mutation of 205T→C was observed through sequencing of different genotypes. AA genotype was dominant in both yellow cattle breeds, and A allele frequency was higher than B allele frequency. X2 test results showed that the two cattle breeds were at Hardy-Weinberg equilibrium state in the digestion site （ P 〉 0.05 ）. [Condusion] The effective molecular markers of Guizbou yellow cattle, such as growth trait and intramuscular fat content, are obtained, which will provide a theoretical basis for mo- lecular breeding of meat performance of Guizhou yellow cattle.
