Tissue culture of Antirrhium majus was experimented by using the tip of shoots as the explants, and comparing the effects of NAA, IAA and 2, 4-D on the formation of adventitious buds. The results indicated that the ef...Tissue culture of Antirrhium majus was experimented by using the tip of shoots as the explants, and comparing the effects of NAA, IAA and 2, 4-D on the formation of adventitious buds. The results indicated that the effect of NAA was the best for the formation of adventitious buds, 5.0, 7.0, 9.0, 11.0, 13.0 mg/L BA combinatcd with 0.1, 0.5, 1.0, 1.5, 2.0 mg/L NAA respectively. The results show that combinations of the concentrations of BA 7.0-9.0 mg/L and NAA 0.1-0.5 mg/L were advantageous to the regeneration of buds and NAA was very important for callus growth.展开更多
An experiment was carried out to examine the effects of light quality on the growth and development of antirrhinum under three different temperatures 19℃, 24℃ and 27℃ in glasshouses. Five different colour filters (...An experiment was carried out to examine the effects of light quality on the growth and development of antirrhinum under three different temperatures 19℃, 24℃ and 27℃ in glasshouses. Five different colour filters (i.e. 'Red absorbing', 'Blue absorbing', 'Blue and Red absorbing' and two 'partially Blue absorbing' materials) were tested, with one clear polythene as a control. Plant height, internode length and leaf area were significantly affected by the spectral filters as well as the temperature.Analysis of color filter's effect on presumed photoreceptors to exist indicated that antirrhinum plant height was regulated by the action of a blue acting photoreceptor (BAP) and not the phytochrome. There was no evidence for an effect ofphytochrome or BAP on time to flowering, however, increasing temperature levels effectively decreased the time to flowering. To predict the effects of different spectral qualities and temperature, simple models were created from data on plant height, internode length and time to flowering. These models were then applied to simulate the potential benefits of spectral filters and temperature in manipulation of growth control and flowering in antirrhinum.展开更多
Shades of different light intensities (29%, 43%, 54%,60% or 68%) along with control (no shade) were studied to observe their effects on the flowering time and plant quality. A hyperbolic relationship was observed betw...Shades of different light intensities (29%, 43%, 54%,60% or 68%) along with control (no shade) were studied to observe their effects on the flowering time and plant quality. A hyperbolic relationship was observed between different light intensities under shade,and time to flowering.The total number of flower buds showed a curvilinear relationship with light intensities. Growth parameters related to the plant characteristics such as plant height,leaf area and plant fresh weight were improved under shading treatments at the expense of flowering time and number of flower buds.However,both linear and polynomial models applied assumed that cultivar Chimes White was equally sensitive to light intensity throughout development.展开更多
Self-incompatibility is an intraspecific reproductive barrier to prevent self-fertilization inthe flowering plants. In many species, self-incompatibility is controlled by a single S locus with multiple alleles. So far...Self-incompatibility is an intraspecific reproductive barrier to prevent self-fertilization inthe flowering plants. In many species, self-incompatibility is controlled by a single S locus with multiple alleles. So far, the only gene known in the S locus of the Solanaceae, Scrophulariaceae and Rosaceae encodes a class of ribonucleases, called self-incompatibility ribonucleases (S RNases), which have been shown to mediate stylar expression of self-incompatible reaction. As the first step to investigate their three-dimensional structure, we successfully expressed three biologically active S RNases of Antirrihnum (S2, S4 and S5) in Escherichia coli (E. coli). Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins. Possible reasons for non-lethality of S RNases on E. coliare discussed.展开更多
为优化温室盆栽金鱼草光温调控精度,以‘红姬’品种为研究对象,通过不同定植期和密度试验,以辐热积(Product of thermal effectiveness and PAR,TEP)为尺度,建立了温室盆栽金鱼草生长发育的模型,并用独立试验数据检验了模型预测精度。...为优化温室盆栽金鱼草光温调控精度,以‘红姬’品种为研究对象,通过不同定植期和密度试验,以辐热积(Product of thermal effectiveness and PAR,TEP)为尺度,建立了温室盆栽金鱼草生长发育的模型,并用独立试验数据检验了模型预测精度。结果表明,模型对萌芽期、展叶期、花蕾期和采收期的时间预测符合度较高,模拟值与实测值基于1:1直线间的决定系数R2为0.95,回归估计标准误差RMSE分别为1.3、1.7、2.3和1.8 d,预测精度明显高于以有效积温为尺度的发育模型(RMSE分别为2.6、2.5、3.9和3.2 d)。模型对株高、茎粗、叶片数、花苞数和干物质量的模拟值与实测值的决定系数R2分别为0.94、0.92、0.96、0.97和0.91,RMSE分别为6.9 cm、0.4 cm、5.8、2.2和1.27 g·株-1。该研究建立的模型能够较准确地预测温室盆栽金鱼草各生育期出现时间、植株形态和干物质生产的动态。建立的模型参数少且易获取,且预测精度较高,可为温室金鱼草生产中种植期、种植密度及环境的优化调控提供理论依据和决策支持。展开更多
文摘Tissue culture of Antirrhium majus was experimented by using the tip of shoots as the explants, and comparing the effects of NAA, IAA and 2, 4-D on the formation of adventitious buds. The results indicated that the effect of NAA was the best for the formation of adventitious buds, 5.0, 7.0, 9.0, 11.0, 13.0 mg/L BA combinatcd with 0.1, 0.5, 1.0, 1.5, 2.0 mg/L NAA respectively. The results show that combinations of the concentrations of BA 7.0-9.0 mg/L and NAA 0.1-0.5 mg/L were advantageous to the regeneration of buds and NAA was very important for callus growth.
文摘An experiment was carried out to examine the effects of light quality on the growth and development of antirrhinum under three different temperatures 19℃, 24℃ and 27℃ in glasshouses. Five different colour filters (i.e. 'Red absorbing', 'Blue absorbing', 'Blue and Red absorbing' and two 'partially Blue absorbing' materials) were tested, with one clear polythene as a control. Plant height, internode length and leaf area were significantly affected by the spectral filters as well as the temperature.Analysis of color filter's effect on presumed photoreceptors to exist indicated that antirrhinum plant height was regulated by the action of a blue acting photoreceptor (BAP) and not the phytochrome. There was no evidence for an effect ofphytochrome or BAP on time to flowering, however, increasing temperature levels effectively decreased the time to flowering. To predict the effects of different spectral qualities and temperature, simple models were created from data on plant height, internode length and time to flowering. These models were then applied to simulate the potential benefits of spectral filters and temperature in manipulation of growth control and flowering in antirrhinum.
文摘Shades of different light intensities (29%, 43%, 54%,60% or 68%) along with control (no shade) were studied to observe their effects on the flowering time and plant quality. A hyperbolic relationship was observed between different light intensities under shade,and time to flowering.The total number of flower buds showed a curvilinear relationship with light intensities. Growth parameters related to the plant characteristics such as plant height,leaf area and plant fresh weight were improved under shading treatments at the expense of flowering time and number of flower buds.However,both linear and polynomial models applied assumed that cultivar Chimes White was equally sensitive to light intensity throughout development.
文摘Self-incompatibility is an intraspecific reproductive barrier to prevent self-fertilization inthe flowering plants. In many species, self-incompatibility is controlled by a single S locus with multiple alleles. So far, the only gene known in the S locus of the Solanaceae, Scrophulariaceae and Rosaceae encodes a class of ribonucleases, called self-incompatibility ribonucleases (S RNases), which have been shown to mediate stylar expression of self-incompatible reaction. As the first step to investigate their three-dimensional structure, we successfully expressed three biologically active S RNases of Antirrihnum (S2, S4 and S5) in Escherichia coli (E. coli). Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins. Possible reasons for non-lethality of S RNases on E. coliare discussed.
