Anxa2 is the most studied member of the calcium-mediated phospholipid-binding protein family annexins and is a biomarker in cancers.In this review,we listed clinical findings and confirmed the value of Anxa2 in early ...Anxa2 is the most studied member of the calcium-mediated phospholipid-binding protein family annexins and is a biomarker in cancers.In this review,we listed clinical findings and confirmed the value of Anxa2 in early diagnosis and prognostic prediction due to its overexpression and adverse effect on the outcome in most tumors.Anxa2 plays a pivotal role in cancer cell proliferation,migration,invasion,metastasis,and treatment resistance.Improved understanding of its cancer-promoting function might make it an ideal target for cancer therapy.Here,we systematically summarized the mechanism of Anxa2 in regulating epithelialmesenchymal transition(EMT),cytoskeleton dynamicity,cell cycle,apoptosis,angiogenesis,and immunology by using various tumor models.These data emphasize the potential of Anxa2 for targeted intervention in tumors.Altering Anxa2 expression,neutralizing the cell surface Anxa2,or inhibiting its activation,such as through Tyr23 phosphorylation,could be considered based on the regulatory mechanism of Anxa2 in tumor progression.展开更多
Objective To explore the interaction of Anxa2 with P-Glycoprotein （P-gp） in the migration and invasion of the multidrug-resistant （MDR） human breast cancer cell line MCF-7/ADR. Methods A pair of short hairpin RNA ...Objective To explore the interaction of Anxa2 with P-Glycoprotein （P-gp） in the migration and invasion of the multidrug-resistant （MDR） human breast cancer cell line MCF-7/ADR. Methods A pair of short hairpin RNA （shRNA） targeting P-gp was transfected into MCF-7/ADR cells, and monoclonal cell strains were screened. The expression of P-gp was detected by Western blot. Transwell chambers were used to observe the cell migration capacity and invasion ability. The interaction between P-gp and Anxa2 was examined by immunoprecipitation and immunofluorescence confocal microscopy analyses. Results P-gp expression was significantly knocked down, and there were notable decreasing trends in the migration and invasion capability of MDR breast cancer cells （P〈0.05）. There was a close interaction between Anxa2 and P-gp. Conclusions MCF-7/ADR is an MDR human breast cancer cell line with high migration and invasion abilities. The knockdown of P-gp notably impaired the migration and invasion abilities of the tumor cells. The interaction of Anxa2 with P-pg may play an important role in time enhanced invasiveness of MDR human breast cancer cells.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 81702992)
文摘Anxa2 is the most studied member of the calcium-mediated phospholipid-binding protein family annexins and is a biomarker in cancers.In this review,we listed clinical findings and confirmed the value of Anxa2 in early diagnosis and prognostic prediction due to its overexpression and adverse effect on the outcome in most tumors.Anxa2 plays a pivotal role in cancer cell proliferation,migration,invasion,metastasis,and treatment resistance.Improved understanding of its cancer-promoting function might make it an ideal target for cancer therapy.Here,we systematically summarized the mechanism of Anxa2 in regulating epithelialmesenchymal transition(EMT),cytoskeleton dynamicity,cell cycle,apoptosis,angiogenesis,and immunology by using various tumor models.These data emphasize the potential of Anxa2 for targeted intervention in tumors.Altering Anxa2 expression,neutralizing the cell surface Anxa2,or inhibiting its activation,such as through Tyr23 phosphorylation,could be considered based on the regulatory mechanism of Anxa2 in tumor progression.
基金supported by grants from the National Natural Science Foundation of China(No.81071731 and 81001188)the Changjiang Scholars and Innovative Research Team(No.IRT1076)the Tianjin Higher Education Science & Technology Fund Planning Project(No.20100120)
文摘Objective To explore the interaction of Anxa2 with P-Glycoprotein （P-gp） in the migration and invasion of the multidrug-resistant （MDR） human breast cancer cell line MCF-7/ADR. Methods A pair of short hairpin RNA （shRNA） targeting P-gp was transfected into MCF-7/ADR cells, and monoclonal cell strains were screened. The expression of P-gp was detected by Western blot. Transwell chambers were used to observe the cell migration capacity and invasion ability. The interaction between P-gp and Anxa2 was examined by immunoprecipitation and immunofluorescence confocal microscopy analyses. Results P-gp expression was significantly knocked down, and there were notable decreasing trends in the migration and invasion capability of MDR breast cancer cells （P〈0.05）. There was a close interaction between Anxa2 and P-gp. Conclusions MCF-7/ADR is an MDR human breast cancer cell line with high migration and invasion abilities. The knockdown of P-gp notably impaired the migration and invasion abilities of the tumor cells. The interaction of Anxa2 with P-pg may play an important role in time enhanced invasiveness of MDR human breast cancer cells.
文摘目的 :采用同位素相对标记和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQi)技术分析耳蜗核(cochlear nucleus,CN)区域特异性膜蛋白。方法 :出生60 d的雄性SD大鼠分为4组,分别是CN、下丘(inferior colliculus,IC)、上橄榄核(superior oliver complex,SOC)、大脑其余部位(Rest)。提取这4个部位组织的细胞膜蛋白,再采用iTRAQi技术检测出CN区域特异性蛋白。通过Uniprot检索这些蛋白的功能和参与的生物代谢过程。结果:本实验最终得到17种CN区域特异性膜蛋白。对CN区域蛋白进行基因本体(gene ontology,GO)分析发现,这些蛋白主要参与生物调节与发育。UNIPROT检索发现3种蛋白与神经系统功能相关,即半乳糖凝集素-1(galectin-1,Gal-1)、膜联蛋白A2(annexin-A2,AXNA2)和外周蛋白(peripherin,PRPH)。Gal-1与神经生长发育相关;AXNA2参与多种生物调节过程;PRPH与神经退行性病变相关。其中,Gal-1可促进轴突生长、调节突触可塑性且具有神经保护作用;AXNA2可能参与了神经递质释放过程,且有神经营养作用;PRPH在高表达时具有神经毒性,和神经丝蛋白共同参与神经退行性病变。结论:Gal-1、ANXA2和PRPH在耳蜗核中高度表达,可能与中枢信息处理相关,这为进一步研究中枢听觉处理障碍发病机制提供了一定的理论基础。
