Organizations are facing unprecedented challenges in the 21st century and OD plays a significant role in creating effective responses to these changes.Not only has it been applied in the USA but gains popularity in dy...Organizations are facing unprecedented challenges in the 21st century and OD plays a significant role in creating effective responses to these changes.Not only has it been applied in the USA but gains popularity in dynamic Asian region under the megatrend of globalization and information technology.This paper gains insight from Asia Organization Development(OD)Network Summit and discusses future potential application of OD to educational systems.展开更多
目的:研究核因子-кB(N F-кB)反义寡核苷酸(A O D N)对TN F-α表达的影响,探讨N F-кB与TN F-α的关系及反义核酸技术作用的特点。方法:将不同浓度经硫代磷酸化修饰的反义寡核苷酸(PS-A O D N)导入培养的R A W264.7细胞中与脂多糖预孵...目的:研究核因子-кB(N F-кB)反义寡核苷酸(A O D N)对TN F-α表达的影响,探讨N F-кB与TN F-α的关系及反义核酸技术作用的特点。方法:将不同浓度经硫代磷酸化修饰的反义寡核苷酸(PS-A O D N)导入培养的R A W264.7细胞中与脂多糖预孵育,设立空白对照组和错义寡核苷酸M O D N()()组。分别采用免疫细胞化学和酶联免疫吸附法检测细胞中N F-κB和TN F-α表达。结果:A O D N组的N F-кB、TN F-α表达较空白对照组及M O D N组明显降低(P<0.05)。结论:反义寡核苷酸在一定时间内能有效地阻抑N F-кB在脂多糖刺激下的表达,从而抑制TN F-α的表达。展开更多
文摘Organizations are facing unprecedented challenges in the 21st century and OD plays a significant role in creating effective responses to these changes.Not only has it been applied in the USA but gains popularity in dynamic Asian region under the megatrend of globalization and information technology.This paper gains insight from Asia Organization Development(OD)Network Summit and discusses future potential application of OD to educational systems.
文摘目的:研究核因子-кB(N F-кB)反义寡核苷酸(A O D N)对TN F-α表达的影响,探讨N F-кB与TN F-α的关系及反义核酸技术作用的特点。方法:将不同浓度经硫代磷酸化修饰的反义寡核苷酸(PS-A O D N)导入培养的R A W264.7细胞中与脂多糖预孵育,设立空白对照组和错义寡核苷酸M O D N()()组。分别采用免疫细胞化学和酶联免疫吸附法检测细胞中N F-κB和TN F-α表达。结果:A O D N组的N F-кB、TN F-α表达较空白对照组及M O D N组明显降低(P<0.05)。结论:反义寡核苷酸在一定时间内能有效地阻抑N F-кB在脂多糖刺激下的表达,从而抑制TN F-α的表达。