Accurate ultra-short-term prediction of the Earth rotation parameters(ERP)holds paramount impor-tance for real-time applications,particularly in reference frame conversion.Among them,diurnal rota-tion(UT1-UTC)which ca...Accurate ultra-short-term prediction of the Earth rotation parameters(ERP)holds paramount impor-tance for real-time applications,particularly in reference frame conversion.Among them,diurnal rota-tion(UT1-UTC)which cannot be directly estimated through Global Navigation Satellite System(GNSS)techniques,significantly affects the rapid and ultra-rapid orbit determination of GNsS satellites.Pres-ently,the traditional LS(least squares)+AR(autoregressive)and LS+MAR(multivariate autoregressive)hybrid methods stand as primary approaches for UT1-UTC ultra-short-term predictions(1-10 days).The LS+MAR hybrid method relies on the UT1-UTC and LOD(length of day)series.However,the correlation between LOD and first-order-difference UT1-UTC is stronger than that between LOD and UT1-UTC.In light of this,and with the aid of the first-order-difference UT1-UTC,we propose an enhanced LS+MAR hybrid method to UT1-UTC ultra-short-term prediction.By using the UT1-UTC and LOD data series of the IERS(International Earth Rotation and Reference Systems Service)EOP 14 C04 product,we conducted a thorough analysis and evaluation of the improved method's prediction performance compared to the traditional LS+AR and LS+MAR hybrid methods.According to the numerical results over more than 210 days,they demonstrate that,when considering the correlation information between the LoD and the first-order-difference UT1-UTC,the mean absolute errors(MAEs)of the improved LS+MAR hybrid method range from 21 to 934μs in 1-10 days predictions.In comparison to the traditional LS+AR hybrid method,the MAEs show a reduction of 7-53μs in 1-10 days predictions,with corresponding improvement percentages ranging from 1 to 28%.Similarly,when compared to the traditional LS+MAR hybrid method,the MAEs have a reduction of 5-42μs in 1-10 days predictions,with corresponding improvement percentages ranging from 4-20%.Additionally,when aided by GNSS-derived LOD data series,the MAEs of improved LS+MAR hybrid method experience further reduction.展开更多
Angiotensin II (Ang II) is the main mediator of the Renin-Angiotensin-System acting on AT<sub>1</sub> and other AT receptors. It is regarded as a pleiotropic agent that induces many actions, including func...Angiotensin II (Ang II) is the main mediator of the Renin-Angiotensin-System acting on AT<sub>1</sub> and other AT receptors. It is regarded as a pleiotropic agent that induces many actions, including functioning as a growth factor, and as a contractile hormone, among others. The aim of this work was to examine the impact of Ang II on the expression and function of α<sub>1</sub>-adrenergic receptors (α<sub>1</sub>-ARs) in cultured rat aorta, and aorta-derived smooth muscle cells. Isolated Wistar rat aorta was incubated for 24 h in DMEM at 37˚C, then subjected to isometric tension and to the action of added norepinephrine, in concentration-response curves. Ang II was added (1 × 10<sup>−5</sup> M), and in some experiments, 5-Methylurapidil (α<sub>1A</sub>-AR antagonist), AH11110A (α<sub>1B</sub>-AR antagonist), or BMY-7378 (α<sub>1D</sub>-AR antagonist), were used to identify the α<sub>1</sub>-AR involved in the response. Desensitization of the contractile response to norepinephrine was observed due to incubation time, and by the Ang II action. α<sub>1D</sub>-AR was protected from desensitization by BMY-7378;while RS-100329 and prazosin partially mitigated desensitization. In another set of experiments, isolated aorta-derived smooth muscle cells were exposed to Ang II and α<sub>1</sub>-ARs proteins were evaluated. α<sub>1D</sub>-AR increased at 30 and 60 min post Ang II exposure, the α<sub>1A</sub>-AR diminished from 1 to 4 h, while α<sub>1B</sub>-AR remained unchanged over 24 h of Ang II exposure. Ang II induced an increase of α<sub>1D</sub>-AR at short times, and BMY-7378 protected α<sub>1D</sub>-AR from desensitization.展开更多
目的探讨三阴型乳腺癌(triple negative breast cancer,TNBC)中AR、SOX10、EGFR及PD-L1的表达及与预后的关系。方法收集557例TNBC患者的临床病理资料,采用免疫组化法检测TNBC中AR、SOX10、EGFR及PD-L1的表达,并复习相关文献。结果557例T...目的探讨三阴型乳腺癌(triple negative breast cancer,TNBC)中AR、SOX10、EGFR及PD-L1的表达及与预后的关系。方法收集557例TNBC患者的临床病理资料,采用免疫组化法检测TNBC中AR、SOX10、EGFR及PD-L1的表达,并复习相关文献。结果557例TNBC中AR、SOX10和EGFR的阳性率分别为16.0%、63.6%和74.7%,AR阳性患者肿瘤直径较小且不易发生转移(P均<0.005),SOX10与EGFR表达与Ki-67增殖指数、组织学类型、分级相关(P均<0.005)。PD-L1阳性率为37.9%,PD-L1表达与肿瘤大小和Ki-67增殖指数相关(P均<0.005),且阳性者多不表达AR,多伴SOX10和EGFR阳性。PD-L1阴性者的无进展生存期(progression-free survival,PFS)比阳性者长,AR和SOX10的表达与患者生存期相关。结论TNBC中AR通常阴性,SOX10和EGFR阳性,PD-L1表达与AR、SOX10、EGFR相关,PD-L1、AR和SOX10的表达和TNBC的PFS相关,有望成为治疗TNBC的新靶点。展开更多
目的探讨癌症和帕金森病相关蛋白DJ-1(parkinson disease protein 7,oncogene DJ-1,DJ-1)下调磷酸酯酶与张力蛋白同源物(phosphatase and tensin homolog,PTEN)诱导雄激素受体(androgen receptor,AR)表达促进非激素依赖性乳腺癌转移的...目的探讨癌症和帕金森病相关蛋白DJ-1(parkinson disease protein 7,oncogene DJ-1,DJ-1)下调磷酸酯酶与张力蛋白同源物(phosphatase and tensin homolog,PTEN)诱导雄激素受体(androgen receptor,AR)表达促进非激素依赖性乳腺癌转移的机制。方法构建人绿色荧光蛋白表达载体pEGFP/DJ-1;Western blotting对比分析过表达DJ-1基因后MDA-MB-231细胞DJ-1、PTEN、雄激素受体(androgen receptor,AR)的表达;体外迁移与侵袭实验检测过表达DJ-1基因后对MDA-MB-231细胞的迁移与侵袭的影响。收集转移的非激素依赖性乳腺癌和未出现转移的非激素依赖性乳腺癌手术切除标本各40例,采用免疫组织化学检测DJ-1蛋白的表达。结果 Western blotting发现经过转染人DJ-1基因的绿色荧光蛋白表达载体pEGFP/DJ-1后,MDA-MB-231细胞DJ-1的表达水平升高,PTEN的表达水平下降,AR水平升高。体外迁移与侵袭实验发现,转染pEGFP/DJ-1促进SWO-38细胞的迁移和侵袭,与转染空白对照组比较,差异有统计学意义(P<0.05)。发生转移非激素依赖性乳腺癌组织中DJ-1阳性表达率(85.0%)明显高于未发生转移乳腺癌组织(55.0%)(P<0.05)。结论 DJ-1能促进非激素依赖性乳腺癌的迁移与侵袭。这可能与DJ-1抑制PTEN表达,提高AR水平从而促进肿瘤细胞的迁移侵袭能力有关。展开更多
基金supported by China Natural Science Fund,China(No.42004016)the science and technology innovation Program of Hunan Province,China(No.2023RC3217)+1 种基金Research Foundation of the Department of Natural Resources of Hunan Province(Grant No:20240105CH)HuBei Natural Science Fund,China(No.2020CFB329).
文摘Accurate ultra-short-term prediction of the Earth rotation parameters(ERP)holds paramount impor-tance for real-time applications,particularly in reference frame conversion.Among them,diurnal rota-tion(UT1-UTC)which cannot be directly estimated through Global Navigation Satellite System(GNSS)techniques,significantly affects the rapid and ultra-rapid orbit determination of GNsS satellites.Pres-ently,the traditional LS(least squares)+AR(autoregressive)and LS+MAR(multivariate autoregressive)hybrid methods stand as primary approaches for UT1-UTC ultra-short-term predictions(1-10 days).The LS+MAR hybrid method relies on the UT1-UTC and LOD(length of day)series.However,the correlation between LOD and first-order-difference UT1-UTC is stronger than that between LOD and UT1-UTC.In light of this,and with the aid of the first-order-difference UT1-UTC,we propose an enhanced LS+MAR hybrid method to UT1-UTC ultra-short-term prediction.By using the UT1-UTC and LOD data series of the IERS(International Earth Rotation and Reference Systems Service)EOP 14 C04 product,we conducted a thorough analysis and evaluation of the improved method's prediction performance compared to the traditional LS+AR and LS+MAR hybrid methods.According to the numerical results over more than 210 days,they demonstrate that,when considering the correlation information between the LoD and the first-order-difference UT1-UTC,the mean absolute errors(MAEs)of the improved LS+MAR hybrid method range from 21 to 934μs in 1-10 days predictions.In comparison to the traditional LS+AR hybrid method,the MAEs show a reduction of 7-53μs in 1-10 days predictions,with corresponding improvement percentages ranging from 1 to 28%.Similarly,when compared to the traditional LS+MAR hybrid method,the MAEs have a reduction of 5-42μs in 1-10 days predictions,with corresponding improvement percentages ranging from 4-20%.Additionally,when aided by GNSS-derived LOD data series,the MAEs of improved LS+MAR hybrid method experience further reduction.
文摘Angiotensin II (Ang II) is the main mediator of the Renin-Angiotensin-System acting on AT<sub>1</sub> and other AT receptors. It is regarded as a pleiotropic agent that induces many actions, including functioning as a growth factor, and as a contractile hormone, among others. The aim of this work was to examine the impact of Ang II on the expression and function of α<sub>1</sub>-adrenergic receptors (α<sub>1</sub>-ARs) in cultured rat aorta, and aorta-derived smooth muscle cells. Isolated Wistar rat aorta was incubated for 24 h in DMEM at 37˚C, then subjected to isometric tension and to the action of added norepinephrine, in concentration-response curves. Ang II was added (1 × 10<sup>−5</sup> M), and in some experiments, 5-Methylurapidil (α<sub>1A</sub>-AR antagonist), AH11110A (α<sub>1B</sub>-AR antagonist), or BMY-7378 (α<sub>1D</sub>-AR antagonist), were used to identify the α<sub>1</sub>-AR involved in the response. Desensitization of the contractile response to norepinephrine was observed due to incubation time, and by the Ang II action. α<sub>1D</sub>-AR was protected from desensitization by BMY-7378;while RS-100329 and prazosin partially mitigated desensitization. In another set of experiments, isolated aorta-derived smooth muscle cells were exposed to Ang II and α<sub>1</sub>-ARs proteins were evaluated. α<sub>1D</sub>-AR increased at 30 and 60 min post Ang II exposure, the α<sub>1A</sub>-AR diminished from 1 to 4 h, while α<sub>1B</sub>-AR remained unchanged over 24 h of Ang II exposure. Ang II induced an increase of α<sub>1D</sub>-AR at short times, and BMY-7378 protected α<sub>1D</sub>-AR from desensitization.
文摘目的探讨三阴型乳腺癌(triple negative breast cancer,TNBC)中AR、SOX10、EGFR及PD-L1的表达及与预后的关系。方法收集557例TNBC患者的临床病理资料,采用免疫组化法检测TNBC中AR、SOX10、EGFR及PD-L1的表达,并复习相关文献。结果557例TNBC中AR、SOX10和EGFR的阳性率分别为16.0%、63.6%和74.7%,AR阳性患者肿瘤直径较小且不易发生转移(P均<0.005),SOX10与EGFR表达与Ki-67增殖指数、组织学类型、分级相关(P均<0.005)。PD-L1阳性率为37.9%,PD-L1表达与肿瘤大小和Ki-67增殖指数相关(P均<0.005),且阳性者多不表达AR,多伴SOX10和EGFR阳性。PD-L1阴性者的无进展生存期(progression-free survival,PFS)比阳性者长,AR和SOX10的表达与患者生存期相关。结论TNBC中AR通常阴性,SOX10和EGFR阳性,PD-L1表达与AR、SOX10、EGFR相关,PD-L1、AR和SOX10的表达和TNBC的PFS相关,有望成为治疗TNBC的新靶点。
文摘目的探讨癌症和帕金森病相关蛋白DJ-1(parkinson disease protein 7,oncogene DJ-1,DJ-1)下调磷酸酯酶与张力蛋白同源物(phosphatase and tensin homolog,PTEN)诱导雄激素受体(androgen receptor,AR)表达促进非激素依赖性乳腺癌转移的机制。方法构建人绿色荧光蛋白表达载体pEGFP/DJ-1;Western blotting对比分析过表达DJ-1基因后MDA-MB-231细胞DJ-1、PTEN、雄激素受体(androgen receptor,AR)的表达;体外迁移与侵袭实验检测过表达DJ-1基因后对MDA-MB-231细胞的迁移与侵袭的影响。收集转移的非激素依赖性乳腺癌和未出现转移的非激素依赖性乳腺癌手术切除标本各40例,采用免疫组织化学检测DJ-1蛋白的表达。结果 Western blotting发现经过转染人DJ-1基因的绿色荧光蛋白表达载体pEGFP/DJ-1后,MDA-MB-231细胞DJ-1的表达水平升高,PTEN的表达水平下降,AR水平升高。体外迁移与侵袭实验发现,转染pEGFP/DJ-1促进SWO-38细胞的迁移和侵袭,与转染空白对照组比较,差异有统计学意义(P<0.05)。发生转移非激素依赖性乳腺癌组织中DJ-1阳性表达率(85.0%)明显高于未发生转移乳腺癌组织(55.0%)(P<0.05)。结论 DJ-1能促进非激素依赖性乳腺癌的迁移与侵袭。这可能与DJ-1抑制PTEN表达,提高AR水平从而促进肿瘤细胞的迁移侵袭能力有关。
