In this study,we aimto investigate certain triple integral transformand its application to a class of partial differentialequations.We discuss various properties of the new transformincluding inversion, linearity, exi...In this study,we aimto investigate certain triple integral transformand its application to a class of partial differentialequations.We discuss various properties of the new transformincluding inversion, linearity, existence, scaling andshifting, etc. Then,we derive several results enfolding partial derivatives and establish amulti-convolution theorem.Further, we apply the aforementioned transform to some classical functions and many types of partial differentialequations involving heat equations,wave equations, Laplace equations, and Poisson equations aswell.Moreover,wedraw some figures to illustrate 3-D contour plots for exact solutions of some selected examples involving differentvalues in their variables.展开更多
This year marks the 20th anniversary of the China-Arab States Cooperation Forum(CASCF),and the tenth Ministerial Meeting of the CASCF was held in Beijing at the end of May,ushering in another highlight for China-Arab ...This year marks the 20th anniversary of the China-Arab States Cooperation Forum(CASCF),and the tenth Ministerial Meeting of the CASCF was held in Beijing at the end of May,ushering in another highlight for China-Arab cooperation.展开更多
目的:研制低致敏复合乳酸菌发酵花生乳。方法:利用试剂盒测定不同品种花生中的粗蛋白含量及Ara h 1含量,选取Ara h 1含量最低的花生品种制作发酵乳;以接菌量、接菌种类、发酵时间及糖添加量为考察因素,Ara h 1含量为测定结果,采用响应...目的:研制低致敏复合乳酸菌发酵花生乳。方法:利用试剂盒测定不同品种花生中的粗蛋白含量及Ara h 1含量,选取Ara h 1含量最低的花生品种制作发酵乳;以接菌量、接菌种类、发酵时间及糖添加量为考察因素,Ara h 1含量为测定结果,采用响应面法优化致敏蛋白Ara h 1含量下降最多的发酵花生乳制备工艺;制作复合益生菌发酵花生乳,并对产品的口感、组织状态和风味进行感官评价。结果:当接菌量为4%、接种嗜热链球菌和保加利亚乳杆菌、发酵4 h、糖添加量为6%时,发酵花生乳中的主要过敏原Ara h 1减少70%,含量为48μg/g,且感官评分达到80分。结论:经工艺优化获得了致敏蛋白Ara h 1含量低,凝乳效果好,口味怡人的复合益生菌发酵花生制品。展开更多
Little information was so far available about allergenic mechanism of the roasted peanut allergens during initial stages of allergy.The purpose of this study was to determine the influence of roasting(150℃,20 min)on ...Little information was so far available about allergenic mechanism of the roasted peanut allergens during initial stages of allergy.The purpose of this study was to determine the influence of roasting(150℃,20 min)on biochemical and biological properties of Ara h 3,a major peanut allergen.Allergenicity of roasted peanut emulsion to mice,differences in uptakes between Ara h 3 purified from raw peanuts(named as Ara h 3-Raw)and that purified from roasted peanuts(named as Ara h 3-Roasted)by bone marrow-derived dendritic cells(BMDCs)and the implication of cell surface receptors involving in uptake,and changes in glycosylation and structure of Ara h 3 after roasting were analyzed in this study.This study suggested that roasting increased allergenicity of peanut to BALB/c mice.Maillard reaction and structural changes of Ara h 3 induced by roasting significantly altered the uptake of Ara h 3-Roasted by BMDCs,and modified Ara h 3 fate in processes involved in immunogenicity and hyper allergenicity,indicating that food processing pattern can change food allergenicity.展开更多
为探究湿热处理对花生球蛋白Ara h 3抗原性的影响,采用硫酸铵分级沉淀法从花生脱脂粉中分离纯化出Ara h 3,进行不同温度、时间和质量浓度的湿热处理,通过正交试验确定最优湿热处理条件,采用间接ELISA法检测湿热处理后Ara h 3抗原性变化...为探究湿热处理对花生球蛋白Ara h 3抗原性的影响,采用硫酸铵分级沉淀法从花生脱脂粉中分离纯化出Ara h 3,进行不同温度、时间和质量浓度的湿热处理,通过正交试验确定最优湿热处理条件,采用间接ELISA法检测湿热处理后Ara h 3抗原性变化,测定外源荧光、游离巯基含量以及圆二色光谱,从蛋白结构的变化解释湿热处理对Ara h 3抗原性的影响。研究表明:湿热处理能够降低Ara h 3抗原性,且在质量浓度10 mg/mL、90℃处理30 min时,抗原性降至最低;湿热处理后的Ara h 3荧光强度、表面疏水性增强,三级结构发生变化;游离巯基含量增加、空间结构发生改变;α-螺旋和β-折叠含量降低,无规则卷曲含量增加,二级结构发生变化。湿热处理后Ara h 3空间结构发生改变,部分抗原表位遭到破坏或被包埋,抗原性降低。展开更多
以Ara h 3为免疫原免疫小鼠。选取血清效价高的小鼠再次免疫,制备出脾细胞后与骨髓瘤细胞一起进行细胞融合,得到杂交瘤细胞株,筛选阳性株制备大量单克隆抗体。通过酶联免疫吸附试验(ELISA)对制备的单克隆抗体进行效价测定、敏感性测定...以Ara h 3为免疫原免疫小鼠。选取血清效价高的小鼠再次免疫,制备出脾细胞后与骨髓瘤细胞一起进行细胞融合,得到杂交瘤细胞株,筛选阳性株制备大量单克隆抗体。通过酶联免疫吸附试验(ELISA)对制备的单克隆抗体进行效价测定、敏感性测定和特异性测定。通过蛋白质免疫印迹试验进一步验证其特异性。结果表明:免疫性较好的4B2单克隆抗体经纯化后的效价为1∶5.12×10^(5)。由敏感性测定得知4B2单克隆抗体的半抑制浓度(IC_(50))为389 ng/mL,证明其具有较好的敏感性,交叉反应率小于0.5%,成功制备出纯度高、效价高、敏感性好、特异性强的Ara h 3鼠源单克隆抗体,为建立免疫学快速检测方法奠定了基础。展开更多
Peanut allergy is considered to be a major health issue with global effects.To date,no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut allergens.The accurate a...Peanut allergy is considered to be a major health issue with global effects.To date,no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut allergens.The accurate and effective detection methods for the surveillance of allergens in food are still the primary strategies to avoid allergic diseases.In this study,nanobodies(Nbs)derived from the Heavy-Chain only Antibodies(HCAbs)were selected against the general peanut protein extract through the unbiased strategy to facilitate the development of the sandwich ELISA for the detection and surveillance of peanut allergen contamination.The target antigen of the selected Nb was identified as peanut allergen Ara h 3,and a cross-reaction was observed with the member of Gly 1 from the Ara h 3 family.The applicability of the self-paired Nb P43 on the establishment of the immuno-assay was verified.A sandwich ELISA against peanut allergen was developed,which reached a linear range of 0.2-10.6μg/mL,and a limit of detection of 53.13 ng/mL.展开更多
Electronic wallet(E-wallet),which combines software,hardware,and human interaction,is a modern platform for electronic payment.Financial services managers can create more effective strategies for advancing existing po...Electronic wallet(E-wallet),which combines software,hardware,and human interaction,is a modern platform for electronic payment.Financial services managers can create more effective strategies for advancing existing positions by evaluating the success of e-wallet services.The objective of this research is to create an integrated model for assessing E-wallet services using the Fuzzy Analytic Hierarchy Process(FAHP)in conjunction with a technique for extent analysis and the ARAS approach(Additive Ratio Assessment).In this study,E-wallet service providers are ranked and evaluated using the ARAS approach and the FAHP priority weights.In addition,a case study from Vietnam illustrates how the methodology is used.The data shows that perceived usefulness,perceived usability,perceived simplicity of use,and perceived threat have the least impact on how acceptable an Ewallet is.As a result,the suggested model improves the efficiency of E-wallet service operations and assists in overcoming the challenges associated with the evaluation of E-wallet services.The case finding demonstrates that the suggested model is a reliable and useful tool for making decisions when assessing E-wallet service providers functioning in a complex and changing environment.展开更多
China-Arab States Tour Operators Conference of the Sixth China-Arab States Expo,co-hosted by the Ministry of Culture and Tourism and the People's Government of Ningxia Hui Autonomous Region,and organized by the Ne...China-Arab States Tour Operators Conference of the Sixth China-Arab States Expo,co-hosted by the Ministry of Culture and Tourism and the People's Government of Ningxia Hui Autonomous Region,and organized by the Network of International Culturalink Entities and the Department of Culture and Tourism of Ningxia Hui Autonomous Region..展开更多
Chinese President Xi Jinping made a trip to Saudi Arabia from December 7 to 10, during which Xi attended the first China-Arab States Summit and the China-Gulf Cooperation Council(GCC) Summit, held bilateral meetings w...Chinese President Xi Jinping made a trip to Saudi Arabia from December 7 to 10, during which Xi attended the first China-Arab States Summit and the China-Gulf Cooperation Council(GCC) Summit, held bilateral meetings with nearly 20 Arab leaders, and paid a state visit to Saudi Arabia.展开更多
To thrive in a world that is undergoing changes not seen in a century,China and Arab states must enhance solidarity and collaboration.CHINA and Arab states are all developing countries.Combined,they account for one-si...To thrive in a world that is undergoing changes not seen in a century,China and Arab states must enhance solidarity and collaboration.CHINA and Arab states are all developing countries.Combined,they account for one-sixth of the world’s land mass,one-fourth of the world’s population,and one-eighth of the world economy.展开更多
Immunoglobulin A nephropathy (IgAN) was first identified and described as a disease by Berger and Hinglais in 1968. It is the most common primary glomerulopathy worldwide [1], most prevalent in East Asians and Caucasi...Immunoglobulin A nephropathy (IgAN) was first identified and described as a disease by Berger and Hinglais in 1968. It is the most common primary glomerulopathy worldwide [1], most prevalent in East Asians and Caucasians and rare in black individuals. There are four key elements that contribute to IgAN, which determine the severity, course, and prognosis of the disease: circulating IgA immunocomplexes that favor mesangial deposition, the efficiency of the reticuloendothelial system, mesangial cell affinity and reaction to mesangial accumulation of poorly glycosylated IgA1, and the renal tendency to glomerulosclerosis and interstitial fibrosis. Clinical manifestations among patients with IgAN include hematuria, approximately 40% to 50% of cases present with one or more episodes of hematuria, usually preceded by upper respiratory tract infections. Between 30% and 40% present with hematuria and non-nephrotic range proteinuria that may be associated with arterial hypertension and impaired renal function. The TESTING study reveal a significant decrease in outcomes such as the risk of a 40% decrease in glomerular filtration rate or the need for renal replacement therapy in the group treated with steroids. The decrease in renal function compared to the group treated in the previously mentioned STOP-IgAN trial was 4 times less than in the TESTING study. Are we doing enough? Obviously, more trials are required with the use of adequate nephroprotection measures. We present 3 patients with a diagnosis of IgA nephropathy who attend the follow-up consultation and voluntarily decide to take part in the review.展开更多
文摘In this study,we aimto investigate certain triple integral transformand its application to a class of partial differentialequations.We discuss various properties of the new transformincluding inversion, linearity, existence, scaling andshifting, etc. Then,we derive several results enfolding partial derivatives and establish amulti-convolution theorem.Further, we apply the aforementioned transform to some classical functions and many types of partial differentialequations involving heat equations,wave equations, Laplace equations, and Poisson equations aswell.Moreover,wedraw some figures to illustrate 3-D contour plots for exact solutions of some selected examples involving differentvalues in their variables.
文摘This year marks the 20th anniversary of the China-Arab States Cooperation Forum(CASCF),and the tenth Ministerial Meeting of the CASCF was held in Beijing at the end of May,ushering in another highlight for China-Arab cooperation.
文摘目的:研制低致敏复合乳酸菌发酵花生乳。方法:利用试剂盒测定不同品种花生中的粗蛋白含量及Ara h 1含量,选取Ara h 1含量最低的花生品种制作发酵乳;以接菌量、接菌种类、发酵时间及糖添加量为考察因素,Ara h 1含量为测定结果,采用响应面法优化致敏蛋白Ara h 1含量下降最多的发酵花生乳制备工艺;制作复合益生菌发酵花生乳,并对产品的口感、组织状态和风味进行感官评价。结果:当接菌量为4%、接种嗜热链球菌和保加利亚乳杆菌、发酵4 h、糖添加量为6%时,发酵花生乳中的主要过敏原Ara h 1减少70%,含量为48μg/g,且感官评分达到80分。结论:经工艺优化获得了致敏蛋白Ara h 1含量低,凝乳效果好,口味怡人的复合益生菌发酵花生制品。
基金funded by the National Key Research and Development Program of China(2016YFD0501101)the project of Food Science Discipline Construction of Shanghai University and the National Natural Science Foundation of China(31201306)。
文摘Little information was so far available about allergenic mechanism of the roasted peanut allergens during initial stages of allergy.The purpose of this study was to determine the influence of roasting(150℃,20 min)on biochemical and biological properties of Ara h 3,a major peanut allergen.Allergenicity of roasted peanut emulsion to mice,differences in uptakes between Ara h 3 purified from raw peanuts(named as Ara h 3-Raw)and that purified from roasted peanuts(named as Ara h 3-Roasted)by bone marrow-derived dendritic cells(BMDCs)and the implication of cell surface receptors involving in uptake,and changes in glycosylation and structure of Ara h 3 after roasting were analyzed in this study.This study suggested that roasting increased allergenicity of peanut to BALB/c mice.Maillard reaction and structural changes of Ara h 3 induced by roasting significantly altered the uptake of Ara h 3-Roasted by BMDCs,and modified Ara h 3 fate in processes involved in immunogenicity and hyper allergenicity,indicating that food processing pattern can change food allergenicity.
文摘为探究湿热处理对花生球蛋白Ara h 3抗原性的影响,采用硫酸铵分级沉淀法从花生脱脂粉中分离纯化出Ara h 3,进行不同温度、时间和质量浓度的湿热处理,通过正交试验确定最优湿热处理条件,采用间接ELISA法检测湿热处理后Ara h 3抗原性变化,测定外源荧光、游离巯基含量以及圆二色光谱,从蛋白结构的变化解释湿热处理对Ara h 3抗原性的影响。研究表明:湿热处理能够降低Ara h 3抗原性,且在质量浓度10 mg/mL、90℃处理30 min时,抗原性降至最低;湿热处理后的Ara h 3荧光强度、表面疏水性增强,三级结构发生变化;游离巯基含量增加、空间结构发生改变;α-螺旋和β-折叠含量降低,无规则卷曲含量增加,二级结构发生变化。湿热处理后Ara h 3空间结构发生改变,部分抗原表位遭到破坏或被包埋,抗原性降低。
文摘以Ara h 3为免疫原免疫小鼠。选取血清效价高的小鼠再次免疫,制备出脾细胞后与骨髓瘤细胞一起进行细胞融合,得到杂交瘤细胞株,筛选阳性株制备大量单克隆抗体。通过酶联免疫吸附试验(ELISA)对制备的单克隆抗体进行效价测定、敏感性测定和特异性测定。通过蛋白质免疫印迹试验进一步验证其特异性。结果表明:免疫性较好的4B2单克隆抗体经纯化后的效价为1∶5.12×10^(5)。由敏感性测定得知4B2单克隆抗体的半抑制浓度(IC_(50))为389 ng/mL,证明其具有较好的敏感性,交叉反应率小于0.5%,成功制备出纯度高、效价高、敏感性好、特异性强的Ara h 3鼠源单克隆抗体,为建立免疫学快速检测方法奠定了基础。
基金financially supported by the grants from the National Key R&D Program of China(No.2019YFC1605005)。
文摘Peanut allergy is considered to be a major health issue with global effects.To date,no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut allergens.The accurate and effective detection methods for the surveillance of allergens in food are still the primary strategies to avoid allergic diseases.In this study,nanobodies(Nbs)derived from the Heavy-Chain only Antibodies(HCAbs)were selected against the general peanut protein extract through the unbiased strategy to facilitate the development of the sandwich ELISA for the detection and surveillance of peanut allergen contamination.The target antigen of the selected Nb was identified as peanut allergen Ara h 3,and a cross-reaction was observed with the member of Gly 1 from the Ara h 3 family.The applicability of the self-paired Nb P43 on the establishment of the immuno-assay was verified.A sandwich ELISA against peanut allergen was developed,which reached a linear range of 0.2-10.6μg/mL,and a limit of detection of 53.13 ng/mL.
基金express their gratitude to PTIT,Vietnam,for financial support for this research.(https://www.ptit.edu.vn/)。
文摘Electronic wallet(E-wallet),which combines software,hardware,and human interaction,is a modern platform for electronic payment.Financial services managers can create more effective strategies for advancing existing positions by evaluating the success of e-wallet services.The objective of this research is to create an integrated model for assessing E-wallet services using the Fuzzy Analytic Hierarchy Process(FAHP)in conjunction with a technique for extent analysis and the ARAS approach(Additive Ratio Assessment).In this study,E-wallet service providers are ranked and evaluated using the ARAS approach and the FAHP priority weights.In addition,a case study from Vietnam illustrates how the methodology is used.The data shows that perceived usefulness,perceived usability,perceived simplicity of use,and perceived threat have the least impact on how acceptable an Ewallet is.As a result,the suggested model improves the efficiency of E-wallet service operations and assists in overcoming the challenges associated with the evaluation of E-wallet services.The case finding demonstrates that the suggested model is a reliable and useful tool for making decisions when assessing E-wallet service providers functioning in a complex and changing environment.
文摘China-Arab States Tour Operators Conference of the Sixth China-Arab States Expo,co-hosted by the Ministry of Culture and Tourism and the People's Government of Ningxia Hui Autonomous Region,and organized by the Network of International Culturalink Entities and the Department of Culture and Tourism of Ningxia Hui Autonomous Region..
文摘Chinese President Xi Jinping made a trip to Saudi Arabia from December 7 to 10, during which Xi attended the first China-Arab States Summit and the China-Gulf Cooperation Council(GCC) Summit, held bilateral meetings with nearly 20 Arab leaders, and paid a state visit to Saudi Arabia.
文摘To thrive in a world that is undergoing changes not seen in a century,China and Arab states must enhance solidarity and collaboration.CHINA and Arab states are all developing countries.Combined,they account for one-sixth of the world’s land mass,one-fourth of the world’s population,and one-eighth of the world economy.
文摘Immunoglobulin A nephropathy (IgAN) was first identified and described as a disease by Berger and Hinglais in 1968. It is the most common primary glomerulopathy worldwide [1], most prevalent in East Asians and Caucasians and rare in black individuals. There are four key elements that contribute to IgAN, which determine the severity, course, and prognosis of the disease: circulating IgA immunocomplexes that favor mesangial deposition, the efficiency of the reticuloendothelial system, mesangial cell affinity and reaction to mesangial accumulation of poorly glycosylated IgA1, and the renal tendency to glomerulosclerosis and interstitial fibrosis. Clinical manifestations among patients with IgAN include hematuria, approximately 40% to 50% of cases present with one or more episodes of hematuria, usually preceded by upper respiratory tract infections. Between 30% and 40% present with hematuria and non-nephrotic range proteinuria that may be associated with arterial hypertension and impaired renal function. The TESTING study reveal a significant decrease in outcomes such as the risk of a 40% decrease in glomerular filtration rate or the need for renal replacement therapy in the group treated with steroids. The decrease in renal function compared to the group treated in the previously mentioned STOP-IgAN trial was 4 times less than in the TESTING study. Are we doing enough? Obviously, more trials are required with the use of adequate nephroprotection measures. We present 3 patients with a diagnosis of IgA nephropathy who attend the follow-up consultation and voluntarily decide to take part in the review.
