期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
拟南芥叶绿体分裂突变体cpd44中ARC6基因的鉴定与分析 被引量:1
1
作者 张琴 贾宁 +1 位作者 李慧 高宏波 《植物生理学报》 CAS CSCD 北大核心 2015年第4期501-508,共8页
叶绿体是光合作用的主要场所,叶绿体的分裂对于维持其正常的功能有着非常重要的作用。本实验从EMS (ethyl methane sulfonate)诱变的拟南芥Columbia生态型(Col)种子中筛选到一个叶绿体分裂突变体cpd44(chloroplast division 44),... 叶绿体是光合作用的主要场所,叶绿体的分裂对于维持其正常的功能有着非常重要的作用。本实验从EMS (ethyl methane sulfonate)诱变的拟南芥Columbia生态型(Col)种子中筛选到一个叶绿体分裂突变体cpd44(chloroplast division 44),其叶绿体的体积显著增大,每个叶肉细胞中的叶绿体数目减少至1~5个。遗传分析显示该表型是单基因控制的隐性突变。运用图位克隆技术和测序分析发现ARC6(accumulation and replication of chloroplasts 6)基因发生突变,第1160个碱基由C突变成T,发生无义突变。半定量RT-PCR分析结果显示cpd44中ARC6的mRNA可能被降解。遗传互补实验进一步证明cpd44的叶绿体异常表型是由ARC6突变造成的。本工作为进一步研究叶绿体分裂机制提供了新材料。 展开更多
关键词 拟南芥 叶绿体分裂 arc6 cpd44
原文传递
The Voltage Distribution Characteristics of a Hybrid Circuit Breaker During High Current Interruption 被引量:7
2
作者 程显 段雄英 +3 位作者 廖敏夫 黄智慧 罗彦 邹积岩 《Plasma Science and Technology》 SCIE EI CAS CSCD 2013年第8期800-806,共7页
Hybrid circuit breaker (HCB) technology based on a vacuum interrupter and a SF6 interrupter in series has become a new research direction because of the low-carbon requirements for high voltage switches. The vacuum ... Hybrid circuit breaker (HCB) technology based on a vacuum interrupter and a SF6 interrupter in series has become a new research direction because of the low-carbon requirements for high voltage switches. The vacuum interrupter has an excellent ability to deal with the steep rising part of the transient recovery voltage (TRV), while the SF6 interrupter can withstand the peak part of the voltage easily. An HCB can take advantage of the interrupters in the current interruption process. In this study, an HCB model based on the vacuum ion diffusion equations, ion density equation, and modified Cassie-Mayr arc equation is explored. A simulation platform is constructed by using a set of software called the alternative transient program (ATP). An HCB prototype is also designed, and the short circuit current is interrupted by the HCB under different action sequences of contacts. The voltage distribution of the HCB is analyzed through simulations and tests. The results demonstrate that if the vacuum interrupter withstands the initial TRV and interrupts the post-arc current first, then the recovery speed of the dielectric strength of the SF6 interrupter will be fast. The voltage distribution between two interrupters is determined by their post-arc resistance, which happens after current-zero, and subsequently, it is determined by the capacitive impedance after the post-arc current decays to zero. 展开更多
关键词 hybrid circuit breaker vacuum arc SF6 arc transient recovery voltage di- electric recovery breaking capacity
下载PDF
Arabidopsis FtsZ2-1 and FtsZ2-2 Are Functionally Redundant, But FtsZ-Based Plastid Division Is Not Essential for Chloroplast Partitioning or Plant Growth and Development 被引量:5
3
作者 Aaron J. Schmitz Jonathan M. Glynn +2 位作者 Bradley J.S.C. Olson Kevin D. Stokes Katherine W. Osteryoung 《Molecular Plant》 SCIE CAS CSCD 2009年第6期1211-1222,共12页
FtsZ1 and FtsZ2 are phylogenetically distinct families of FtsZ in plants that co-localize to mid-plastid rings and facilitate division of chloroplasts. In plants, altered levels of either FtsZ1 or FtsZ2 cause dose-dep... FtsZ1 and FtsZ2 are phylogenetically distinct families of FtsZ in plants that co-localize to mid-plastid rings and facilitate division of chloroplasts. In plants, altered levels of either FtsZ1 or FtsZ2 cause dose-dependent defects in chloroplast division; thus, studies on the functional relationship between FtsZgenes require careful manipulation of FtsZ levels in vivo. To define the functional relationship between the two FtsZ2 genes in Arabidopsis thaliana, FtsZ2-1 and FtsZ2-2, we expressed FtsZ2-1 in an ftsZ2-2 null mutant, and vice versa, and determined whether the chloroplast division defects were rescued in plants expressing different total levels of FtsZ2. Full rescue was observed when either the FtsZ2-1 or FtsZ2-2 level approximated total FtsZ2 levels in wild-type (WT). Additionally, FtsZ2-2 interacts with ARC6, as shown previously for FtsZ2- 1. These data indicate that FtsZ2-1 and FtsZ2-2 are functionally redundant for chloroplast division in Arabidopsis. To rigorously validate the requirement of each FtsZ family for chloroplast division, we replaced FtsZ1 with FtsZ2 in vivo, and vice versa, while maintaining the FtsZ level in the transgenic plants equal to that of the total level in WT. Chloroplast division defects were not rescued, demonstrating conclusively that FtsZ1 and FtsZ2 are non-redundant for maintenance of WT chloroplast numbers. Finally, we generated ftsZtriple null mutants and show that plants completely devoid of FtsZ protein are viable and fertile. As plastids are presumably essential organelles, these findings suggest that an FtsZ-independent mode of plastid partitioning may occur in higher plants. 展开更多
关键词 CHLOROPLAST plastid division FTSZ arc6 PDV1.
原文传递
Microstructure and Tribological Behavior of a TiO_2/hBN Composite Ceramic Coating Formed via Micro-arc Oxidation of Ti–6Al–4V Alloy 被引量:21
4
作者 Ni Ao Daoxin Liu +3 位作者 Shuaixing Wang Qing Zhao Xiaohua Zhang Mengmeng Zhang 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2016年第10期1071-1076,共6页
A composite coating containing hexagonal boron nitride(hBN) particles and titanium oxide(TiO_2) was formed on the surface of Ti-6Al-4V alloy via micro-arc oxidation(MAO). The effect of quantity of the hBN-partic... A composite coating containing hexagonal boron nitride(hBN) particles and titanium oxide(TiO_2) was formed on the surface of Ti-6Al-4V alloy via micro-arc oxidation(MAO). The effect of quantity of the hBN-particles added into electrolyte on microstructure, composition, and wear behavior of the resulting composite coatings was investigated. Microstructure, phase composition, and tribological behavior of the resulting MAO coatings were evaluated via scanning electron microscopy, X-ray diffraction, and ball-on-disc abrasive tests. The results reveal that the TiO_2/hBN composite coating consisting of rutile TiO_2, anatase TiO_2, and an hBN phase was less porous than particle-free coating. Furthermore, the presence of hBN particles in the MAO coating produced an improved anti-friction property. The composite coating produced in the electrolyte containing 2 g/L of hBN particles exhibited the best wear resistance.The outer loose layer of the MAO coatings was removed by a mechanical polishing process, which led to a significant improvement in the wear resistance and anti-friction properties of the MAO coatings and highlighted an essential lubricating role of hBN particles in the composite coatings. However, wear mechanism of the MAO coatings was not relevant to the presence of hBN particles, where fatigue wear dominated the anti-fraction properties of the MAO coatings with and without hBN particles. 展开更多
关键词 Ti-6Al-4V alloy Micro arc oxidation hBN particle Wear
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部