[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Meth...[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.展开更多
Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions...Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions of A.arguta were measured,and sensory attributes including olfactive intensity,juiciness,sweetness,sourness,and astringency were assessed.Cluster analysis was carried out based on mineral elements,and principal component analysis(PCA)was used for comprehensive score ranking.The result demonstrated that fruit weight ranged from 3.5 g to 24.2 g,SSC ranged from 11.94%to 20.56%,TAC ranged from 0.15%to 1.20%,and vitamin C ranged from 3.51 g/kg to 230.76 g/kg(fresh weight)among the tested samples.There were significant differences in sourness,sweetness,bitterness,and umami among the 67 accessions based on electronic tongue analysis,and this result was rapidly and objectively characterized by the sensory assessment.The dendrogram was obtained and clustered the accessions into two major clusters,and five principal components were extracted by PCA.Overall,the information provided by this study identified the 10 best germplasm resources for fresh-eating and processing fruits.It can provide theoretical reference for nutritional efficacy evaluation and food development of A.arguta.展开更多
[Objectives] This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No. 1 as the test material.[Methods]Huanyou No. 1 was sprayed with chitin,and the changes of som...[Objectives] This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No. 1 as the test material.[Methods]Huanyou No. 1 was sprayed with chitin,and the changes of some physiological and biochemical indexes in fruit softening were investigated. [Results]Chitin treatment significantly improved single fruit weight,increased soluble solid content,but decreased titratable acid content and significantly reduced the content of cellulose and hemicellulose; and the chitin treatment significantly inhibited the breakdown of pectin,cellulose and hemicellulose,and inhibited the activities of PG enzyme and β-Gal enzyme. [Conclusions] Chitin spray treatment could increase the yield of A. arguta,and improve its quality and storage property.展开更多
[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid rat...[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power as influencing factors.[Results]The optimal extraction process was as follows:solid/liquid ratio 1∶15(g/mL),ultrasonic temperature 50℃,ultrasonic time 50 min and ultrasonic power 90 W.Under the optimal extraction conditions,the extraction rate of polysaccharides reached 2.05%.[Conclusions]The method is simple and feasible,and can be used to optimize the extraction process of polysaccharides in A.arguta,in order to provide a theoretical basis for the further development and utilization of related resources.展开更多
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest (201303093)the Key Project for Agricultural Improved Variety Breeding of Shandong Province (Lunongliang[2016]No.6~~
文摘[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.
基金supported by Special Scientific Research Fund of Agricultural Public Welfare Profession of China[grant number 200903013]Liaoning Province,Shenyang Agricultural University,high-end talent introduction fund project[grant number SYAU20160003]。
文摘Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions of A.arguta were measured,and sensory attributes including olfactive intensity,juiciness,sweetness,sourness,and astringency were assessed.Cluster analysis was carried out based on mineral elements,and principal component analysis(PCA)was used for comprehensive score ranking.The result demonstrated that fruit weight ranged from 3.5 g to 24.2 g,SSC ranged from 11.94%to 20.56%,TAC ranged from 0.15%to 1.20%,and vitamin C ranged from 3.51 g/kg to 230.76 g/kg(fresh weight)among the tested samples.There were significant differences in sourness,sweetness,bitterness,and umami among the 67 accessions based on electronic tongue analysis,and this result was rapidly and objectively characterized by the sensory assessment.The dendrogram was obtained and clustered the accessions into two major clusters,and five principal components were extracted by PCA.Overall,the information provided by this study identified the 10 best germplasm resources for fresh-eating and processing fruits.It can provide theoretical reference for nutritional efficacy evaluation and food development of A.arguta.
文摘[Objectives] This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No. 1 as the test material.[Methods]Huanyou No. 1 was sprayed with chitin,and the changes of some physiological and biochemical indexes in fruit softening were investigated. [Results]Chitin treatment significantly improved single fruit weight,increased soluble solid content,but decreased titratable acid content and significantly reduced the content of cellulose and hemicellulose; and the chitin treatment significantly inhibited the breakdown of pectin,cellulose and hemicellulose,and inhibited the activities of PG enzyme and β-Gal enzyme. [Conclusions] Chitin spray treatment could increase the yield of A. arguta,and improve its quality and storage property.
基金National Undergraduate Innovation and Entrepreneurship Training Program of China(202111430049).
文摘[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power as influencing factors.[Results]The optimal extraction process was as follows:solid/liquid ratio 1∶15(g/mL),ultrasonic temperature 50℃,ultrasonic time 50 min and ultrasonic power 90 W.Under the optimal extraction conditions,the extraction rate of polysaccharides reached 2.05%.[Conclusions]The method is simple and feasible,and can be used to optimize the extraction process of polysaccharides in A.arguta,in order to provide a theoretical basis for the further development and utilization of related resources.
