Coronary arteries supply blood and nutrients to the heart. Coronary Artery Disease (CAD) develops by narrowing of the arteries. Once the inner walls of the arteries are damaged, fatty deposits made of cells, connectiv...Coronary arteries supply blood and nutrients to the heart. Coronary Artery Disease (CAD) develops by narrowing of the arteries. Once the inner walls of the arteries are damaged, fatty deposits made of cells, connective tissue elements, lipids and debris decrease their lumens which block blood flow to distal tissues. This process is called arteriosclerosis. This study intends to detect <span>the composition of human coronary atheroma collected from the arteries during</span> bypass surgeries in Bangladesh. It shows that all lesions are present in the advanced types, namely, type 4, 5 and 6. Infrequently thrombotic materials and necrotic debris were found in type 6 plaque. The mean length of plaque was 3.36 ± 1.71 cm and mean weight was 0.13 ± 0.12 gm. Insufficient lipid was obtained from each atheroma. Few raised areas were lipid laden whereas <span>most of the strands were fibrous. Biochemical assessment was formidable. The</span> cholesterol composition of coronary plaque ranged between 0.20% - 9.83% with mean 3.06% ± 2.09% and total fatty acids ranged between 0.47% - 3.04% with mean 1.31% ± 0.63%. The most abundant fatty acid was oleic acid (unsaturated) closely followed by palmitic acid (saturated). The mean calcium content was 41,180.20 ± 34,918.59 ppm and ranged from 1148.00 - 140,311.00 ppm or, 0.115% - 14.031%. This study was undertaken to detect the composition of human coronary atheroma in Bangladesh which revealed that it depended upon the type of lesion.展开更多
Background Our previous research has suggested that platelet activating factor receptor was related to atherosclerosis. The present study investigated the effect of a platelet activating factor receptor antagonist- WE...Background Our previous research has suggested that platelet activating factor receptor was related to atherosclerosis. The present study investigated the effect of a platelet activating factor receptor antagonist- WEB2086 on angiogenesis in aortal plaque and ischemic hindlimb of apolipoprotein E-deficient mice. Methods Eight-week-old apolipoprotein E-deficient mice were fed with a 0.15% cholesterol diet to develop advanced lesions. At age 32 weeks unilateral hindlimb ischemia was surgically induced and the mice were divided into two groups： with or without WEB2086 mixed with their drinking water （4.3 mg in 100 ml）. At age 40 weeks blood was collected from the orbit for measurement of serum lipids and an enzyme linked immunosorbent assay was used to determine platelet activating factor and oxidized low density lipoprotein in the gastrocnemius and aorta. Whole-Mount CD31 stain and plaque-associated sprouting have been used to estimate angiogenesis in plaque from the aorta and laser Doppler perfusion imaging and immunohistochemical expression of von Willebrand factor have been used to estimate angiogenesis in ischemic hindlimb. Results The lipid composition of serum was not different between the groups. However, the amount of platelet activating factor and oxidized low density lipoprotein detected in the aorta was significantly higher than that in the gastrocnemius of ischemic hindlimb. The ratio of lesion to aorta levels was significantly reduced by administration of WEB2086, （31.52±6.18）% vs （55.58±8.34）%, P〈0.01. The mean density of intimal capillaries in atherosclerotic plaque, （31.13±9.20）% vs （57.74±11.28）%, P〈0.01, and the mean number of sprouts per aorta were significantly reduced, 183.92±34.17 vs 392.54±76.79, P〈0.01, in the WEB2086 group. Blood flow （0.85±0.12 vs 0.45±0.06, P〈0.01） and capillary density of ischemic hindlimb （1.18±0.17 vs 0.53±0.09, P〈0.01） were markedly increased in apolipoprotein E-deficient mice treated with WEB2086 versus controls. Conclusion The study provides evidence that WEB2086 can inhibit angiogenesis in atherosclerotic plaque but promote it in ischemic hindlimb.展开更多
文摘Coronary arteries supply blood and nutrients to the heart. Coronary Artery Disease (CAD) develops by narrowing of the arteries. Once the inner walls of the arteries are damaged, fatty deposits made of cells, connective tissue elements, lipids and debris decrease their lumens which block blood flow to distal tissues. This process is called arteriosclerosis. This study intends to detect <span>the composition of human coronary atheroma collected from the arteries during</span> bypass surgeries in Bangladesh. It shows that all lesions are present in the advanced types, namely, type 4, 5 and 6. Infrequently thrombotic materials and necrotic debris were found in type 6 plaque. The mean length of plaque was 3.36 ± 1.71 cm and mean weight was 0.13 ± 0.12 gm. Insufficient lipid was obtained from each atheroma. Few raised areas were lipid laden whereas <span>most of the strands were fibrous. Biochemical assessment was formidable. The</span> cholesterol composition of coronary plaque ranged between 0.20% - 9.83% with mean 3.06% ± 2.09% and total fatty acids ranged between 0.47% - 3.04% with mean 1.31% ± 0.63%. The most abundant fatty acid was oleic acid (unsaturated) closely followed by palmitic acid (saturated). The mean calcium content was 41,180.20 ± 34,918.59 ppm and ranged from 1148.00 - 140,311.00 ppm or, 0.115% - 14.031%. This study was undertaken to detect the composition of human coronary atheroma in Bangladesh which revealed that it depended upon the type of lesion.
基金This work was supported by Scientific Research Fund of Hunan Provincial Education Department(No.05C457).
文摘Background Our previous research has suggested that platelet activating factor receptor was related to atherosclerosis. The present study investigated the effect of a platelet activating factor receptor antagonist- WEB2086 on angiogenesis in aortal plaque and ischemic hindlimb of apolipoprotein E-deficient mice. Methods Eight-week-old apolipoprotein E-deficient mice were fed with a 0.15% cholesterol diet to develop advanced lesions. At age 32 weeks unilateral hindlimb ischemia was surgically induced and the mice were divided into two groups： with or without WEB2086 mixed with their drinking water （4.3 mg in 100 ml）. At age 40 weeks blood was collected from the orbit for measurement of serum lipids and an enzyme linked immunosorbent assay was used to determine platelet activating factor and oxidized low density lipoprotein in the gastrocnemius and aorta. Whole-Mount CD31 stain and plaque-associated sprouting have been used to estimate angiogenesis in plaque from the aorta and laser Doppler perfusion imaging and immunohistochemical expression of von Willebrand factor have been used to estimate angiogenesis in ischemic hindlimb. Results The lipid composition of serum was not different between the groups. However, the amount of platelet activating factor and oxidized low density lipoprotein detected in the aorta was significantly higher than that in the gastrocnemius of ischemic hindlimb. The ratio of lesion to aorta levels was significantly reduced by administration of WEB2086, （31.52±6.18）% vs （55.58±8.34）%, P〈0.01. The mean density of intimal capillaries in atherosclerotic plaque, （31.13±9.20）% vs （57.74±11.28）%, P〈0.01, and the mean number of sprouts per aorta were significantly reduced, 183.92±34.17 vs 392.54±76.79, P〈0.01, in the WEB2086 group. Blood flow （0.85±0.12 vs 0.45±0.06, P〈0.01） and capillary density of ischemic hindlimb （1.18±0.17 vs 0.53±0.09, P〈0.01） were markedly increased in apolipoprotein E-deficient mice treated with WEB2086 versus controls. Conclusion The study provides evidence that WEB2086 can inhibit angiogenesis in atherosclerotic plaque but promote it in ischemic hindlimb.
